MARCHF5 (NM_017824) Human Tagged ORF Clone
CAT#: RG215645
- TrueORF®
41338 (tGFP-tagged) - Human membrane-associated ring finger (C3HC4) 5 (MARCH5)
ORF Plasmid: DDK
"NM_017824" in other vectors (4)
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CNY 4,000.00
CNY 4,370.00
Specifications
Product Data | |
Type | Human Tagged ORF Clone |
Tag | TurboGFP |
Synonyms | MARCH-V; MARCH5; MITOL; RNF153 |
Vector | pCMV6-AC-GFP |
E. coli Selection | Ampicillin (100 ug/mL) |
Mammalian Cell Selection | Neomycin |
Sequence Data |
>RG215645 representing NM_017824
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGCCGGACCAAGCCCTACAGCAGATGCTGGACAGAAGTTGCTGGGTTTGTTTTGCTACTGATGAAGATG ATAGAACAGCTGAATGGGTGAGACCATGCAGGTGCAGAGGATCTACAAAATGGGTTCACCAGGCCTGTCT ACAACGCTGGGTGGATGAAAAGCAAAGAGGAAACAGTACAGCCAGAGTGGCATGTCCTCAGTGCAATGCT GAATACCTAATAGTTTTTCCAAAATTGGGTCCAGTGGTTTACGTCTTGGATCTTGCAGATAGACTGATCT CAAAAGCCTGTCCATTTGCTGCAGCAGGAATAATGGTCGGCTCTATCTATTGGACAGCTGTGACTTATGG AGCAGTGACAGTGATGCAGGTTGTAGGTCATAAAGAAGGTCTGGATGTTATGGAGAGAGCTGATCCTTTA TTCCTTTTAATTGGACTTCCTACTATTCCTGTCATGCTGATATTAGGCAAGATGATTCGCTGGGAGGACT ATGTGCTTAGACTGTGGCGCAAATACTCGAATAAACTACAAATTTTAAATAGTATATTTCCAGGGATAGG TTGTCCTGTTCCTCGAATTCCAGCTGAGGCCAATCCTTTAGCAGATCATGTCTCTGCTACTCGAATCTTG TGTGGAGCCCTTGTCTTTCCTACTATTGCTACAATAGTTGGTAAATTGATGTTCAGTAGTGTTAACTCTA ATTTACAAAGGACAATCTTGGGTGGAATTGCGTTTGTTGCCATAAAAGGAGCATTTAAAGTTTACTTCAA ACAGCAGCAATATTTACGACAGGCACACCGCAAAATTCTGAATTATCCAGAACAAGAAGAAGCA ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA >RG215645 representing NM_017824
Red=Cloning site Green=Tags(s) MPDQALQQMLDRSCWVCFATDEDDRTAEWVRPCRCRGSTKWVHQACLQRWVDEKQRGNSTARVACPQCNA EYLIVFPKLGPVVYVLDLADRLISKACPFAAAGIMVGSIYWTAVTYGAVTVMQVVGHKEGLDVMERADPL FLLIGLPTIPVMLILGKMIRWEDYVLRLWRKYSNKLQILNSIFPGIGCPVPRIPAEANPLADHVSATRIL CGALVFPTIATIVGKLMFSSVNSNLQRTILGGIAFVAIKGAFKVYFKQQQYLRQAHRKILNYPEQEEA TRTRPLE - GFP Tag - V |
Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
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ACCN | NM_017824 |
ORF Size | 834 bp |
OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
Reference Data | |
RefSeq | NM_017824.5 |
RefSeq Size | 3941 bp |
RefSeq ORF | 837 bp |
Locus ID | 54708 |
UniProt ID | Q9NX47 |
Domains | RING |
Protein Families | Transmembrane |
Gene Summary | MARCH5 is a ubiquitin ligase of the mitochondrial outer membrane that plays a role in the control of mitochondrial morphology by regulating mitofusin-2 (MFN2; MIM 608507) and DRP1 (DNM1L; MIM 603850) (Nakamura et al., 2006 [PubMed 16936636]).[supplied by OMIM, Mar 2008] |
Documents
Product Manuals |
FAQs |
SDS |
Resources
Other Versions
SKU | Description | Size | Price |
---|---|---|---|
RC215645 | MARCH5 (Myc-DDK-tagged)-Human membrane-associated ring finger (C3HC4) 5 (MARCH5) |
CNY 2,400.00 |
|
RC215645L3 | Lenti ORF clone of Human membrane-associated ring finger (C3HC4) 5 (MARCH5), Myc-DDK-tagged |
CNY 5,890.00 |
|
RC215645L4 | Lenti ORF clone of Human membrane-associated ring finger (C3HC4) 5 (MARCH5), mGFP tagged |
CNY 5,890.00 |
|
SC113977 | 41338 (untagged)-Human membrane-associated ring finger (C3HC4) 5 (MARCH5) |
CNY 2,400.00 |
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