MARCHF5 (NM_017824) Human Untagged Clone

CAT#: SC113977

41338 (untagged)-Human membrane-associated ring finger (C3HC4) 5 (MARCH5)



  "NM_017824" in other vectors (4)

CNY 2,400.00

CNY 6,270.00


货期*
现货

规格
    • 10 ug

Product images

经常一起买 (4)
TurboFectin Transfection Reagent (1 mL in 1 vial)
    • 1 ml in 1 vial

CNY 4,090.00


DH5α Chemically Competent Cells (≥10^8 cfu/μg of pUC19 DNA)
    • 5 x 200 ul

CNY 1,280.00


Forward sequencing primer VP1.5, Reverse sequencing primer XL39, 100pmoles each
    • 100 pmol

CNY 480.00


Rabbit polyclonal anti-MARCH5 antibody
    • 100 ul

CNY 1,999.00
CNY 3,280.00

Specifications

Product Data
Type Human Untagged Clone
Tag Tag Free
Synonyms MARCH-V; MARCH5; MITOL; RNF153
Vector pCMV6-XL5
E. coli Selection Ampicillin (100 ug/mL)
Mammalian Cell Selection None
Sequence Data
>OriGene ORF within SC113977 sequence for NM_017824 edited (data generated by NextGen Sequencing)
ATGCCGGACCAAGCCCTACAGCAGATGCTGGACAGAAGTTGCTGGGTTTGTTTTGCTACT
GATGAAGATGATAGAACAGCTGAATGGGTGAGACCATGCAGGTGCAGAGGATCTACAAAA
TGGGTTCACCAGGCCTGTCTACAACGCTGGGTGGATGAAAAGCAAAGAGGAAACAGTACA
GCCAGAGTGGCATGTCCTCAGTGCAATGCTGAATACCTAATAGTTTTTCCAAAATTGGGT
CCAGTGGTTTACGTCTTGGATCTTGCAGATAGACTGATCTCAAAAGCCTGTCCATTTGCT
GCAGCAGGAATAATGGTCGGCTCTATCTATTGGACAGCTGTGACTTATGGAGCAGTGACA
GTGATGCAGGTTGTAGGTCATAAAGAAGGTCTGGATGTTATGGAGAGAGCTGATCCTTTA
TTCCTTTTAATTGGACTTCCTACTATTCCTGTCATGCTGATATTAGGCAAGATGATTCGC
TGGGAGGACTATGTGCTTAGACTGTGGCGCAAATACTCGAATAAACTACAAATTTTAAAT
AGTATATTTCCAGGGATAGGTTGTCCTGTTCCTCGAATTCCAGCTGAGGCCAATCCTTTA
GCAGATCATGTCTCTGCTACTCGAATCTTGTGTGGAGCCCTTGTCTTTCCTACTATTGCT
ACAATAGTTGGTAAATTGATGTTCAGTAGTGTTAACTCTAATTTACAAAGGACAATCTTG
GGTGGAATTGCGTTTGTTGCCATAAAAGGAGCATTTAAAGTTTACTTCAAACAGCAGCAA
TATTTACGACAGGCACACCGCAAAATTCTGAATTATCCAGAACAAGAAGAAGCATAA

Clone variation with respect to NM_017824.4
>OriGene 5' read for NM_017824 unedited
AACCGCGATTTTGTAAACCCCGACTTCACTATNAGGGCGGCACGCGCAATTCGGCACGAG
GCCGGCAGCAACTCGGCGCCCGCGGTCCATGGACCGGAACCTCGGGCCGACGGACGGGAA
CCCGGGCCGCGATCGCCGCCTCCCCGCCTCAGGCTCCTCCTCCTCGCTCTCCGCCGCCTC
CGCCGGACTCCCGCAGGCCCTGCACCGCCGCCGCCAGGCTAGCTTTATCTGCCCCGGGAA
GCTGGGTGACGGGTTCGCGGCTGCCGCCGGACTGCGGCCTACTCCGCCGCCTCTCAGTGC
TATTGTCCCTGGGCCTGGCCTTGAGCGGGTCCACTGGGGAAGGCCGTGTGCGCCGGCTCC
GCGGAAGATGCCGGACCAAGCCCTACAGCAGATGCTGGACAGAAGTTGCTGGGTTTGTTT
TGCTACTGATGAAGATGATAGAACAGCTGAATGGGTGAGACCATGCAGGTGCAGAGGATC
TACAAAATGGGTTCACCAGGCCTGTCTACAACGCTGGGTGGATGAAAAGCANAGAGGAAA
CAGTACAGCCAGAGTGGCATGTCCTCAGTGCAATGCTGAATACCTAATAGTTTTTCCAAA
ATTGGGTCCAGTGGTTTACGTCTTGGATCTTGCAGATAGACTGATCTCAAAAGCCTGTCC
ATTTGCTGCAGCAGGAATAATGGTCGGCTCTATCTATTGGACAGCTGTGACTTATGGAGC
AGTGACAGTGATGCAGGTTGTAAGTCATAAAAAAGGTCTGGATGTTATGGAGAGAGCTGA
TCCTTTATTCCTTTTAATTGGACTTCCTACTATTCCTGTCATGCTGATATTAAGCAAGAT
GATTCGCTGGGAGGACTATGTGCTTAGACTGTGGCGCNATACTCCGAATAACTACAAATT
TTAATAGTATATTTCCAGGGATAGGTTGTCCCGGTCCTCGAATCN
>OriGene 3' read for NM_017824 unedited
CCGCGGGCCGCAATCTAAAGTCGAGTTTTTTTTTTTTTTTTTTGAGAGTAAAATAACTTT
ATTTTTATTTTTGCAAATCATCCTCTGCTCAAACTACTATAAACTGGCAGCTGTAATTTC
TAGAAATATGGGTTGAAAAAACTCCCAGAGATGGAAGGACCTGATGAAACACTGAAACAC
ACAAATACACACACACACACACACACACACACACACACACACGACTGCATGAGTCAAAAT
TACTTCATGAAGTATGAGATGTCAACATTATGTAAATAAAATTTAATATTGAAAAAGTGA
TCTGGAAGCCCTGTGAATTTTTACCCTACACATTTTGGAGGACATCACATGGGTTAAAGA
AAACACCACAAAATCTCATTTGTTCAGATAATCATTTGAATGTTTACATACATTTTTGGT
TATTTATTTATAACTAATTTAATTTACATTTCCTCATATTAACATATCCTCTTATTTAGT
ATGTAATCAAATTATGTCAGTGAGAAAAATCCTCATAGTACCTTGAGTTGCTGATTCTGA
GAAAACACTAAACATGAACATGTACAAAAGAGGCATTTGAATAAAACAGCATGTGCATTT
TTTTTAATATTTGCACAGTTTGATCTTTGCATTTCTGTCTGGAGAAAATATGAACAAAAC
CCAGGTTTACCATGCTAAGATAATGGTTCATATGACTGCCATANNAGTAGTACATACAAA
CAATGAANNACAATCACATCCTTAAGTTCCATGATTACCGAGATGATTTCCCCTGCCCTA
AGTGCTTTATTATATTTCTTAAAAATATANGAGCAGCAGCTTATCACAAGTCTNCACAAG
TGCATTTATGATGGNATCANNNACACACACAGATTCTAGGATGAGAGACTGGCGACACCA
GAANCAGTTTATGCTTCTCCTGTTCTGATAATCAGAATTTGCGGGTGCC
Restriction Sites NotI-NotI     
ACCN NM_017824
Insert Size 1960 bp
OTI Disclaimer Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq NM_017824.4, NP_060294.1
RefSeq Size 3941 bp
RefSeq ORF 837 bp
Locus ID 54708
UniProt ID Q9NX47
Domains RING
Protein Families Transmembrane
Gene Summary MARCH5 is a ubiquitin ligase of the mitochondrial outer membrane that plays a role in the control of mitochondrial morphology by regulating mitofusin-2 (MFN2; MIM 608507) and DRP1 (DNM1L; MIM 603850) (Nakamura et al., 2006 [PubMed 16936636]).[supplied by OMIM, Mar 2008]
*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

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