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ELYS (AHCTF1) (NM_015446) Human Tagged ORF Clone
CAT＃: RG215742
- TrueORF®
AHCTF1 (tGFP-tagged) - Human AT hook containing transcription factor 1 (AHCTF1)
ORF Plasmid: DDK
"NM_015446" in other vectors (2)
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CNY 17,860.00
货期*
12周
规格
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经常一起买 (5)
Specifications
| Product Data | |
| Type | Human Tagged ORF Clone |
| Tag | TurboGFP |
| Synonyms | ELYS; MST108; MSTP108; TMBS62 |
| Vector | pCMV6-AC-GFP |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>RG215742 representing NM_015446
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGCGAGACTTAAGAGCTCAAGTGACTAGTGGTCTCCTGCCATTTCCAGAAGTGACTCTTCAAGCCCTTG GAGAAGACGAAATAACATTAGAATCTGTGCTTCGTGGAAAGTTTGCTGCAGGGAAAAATGGACTTGCTTG CTTGGCTTGTGGTCCACAACTTGAGGTAGTAAACTCTATAACAGGAGAGCGATTGTCTGCTTACAGATTC AGTGGAGTCAATGAACAGCCTCCTGTAGTTTTAGCTGTGAAAGAATTCTCTTGGCAGAAGAGAACTGGAT TATTAATAGGATTGGAAGAAACAGAAGGGAGTGTTCTCTGTCTTTATGACCTTGGAATATCAAAAGTAGT TAAAGCAGTTGTTCTTCCTGGAAGGGTAACAGCTATTGAACCTATAATTAATCATGGAGGAGCCAGTGCA AGCACTCAGCATTTACATCCAAGTCTGCGATGGCTTTTTGGAGTGGCAGCTGTGGTCACTGATGTTGGAC AGATCCTTCTTGTTGACCTATGTTTGGATGACTTGTCATGCAATCAAAATGAAGTTGAAGCATCAGATCT TGAAGTTCTAACTGGTATCCCAGCTGAAGTACCACACATTAGAGAAAGTGTAATGAGACAAGGGCGCCAT CTGTGTTTCCAGTTAGTAAGTCCAACAGGAACAGCTGTTTCAACTCTTAGTTACATAAGCAGGACAAATC AGCTTGCTGTAGGTTTTTCTGATGGCTATCTAGCACTTTGGAACATGAAAAGCATGAAAAGAGAATATTA CATACAATTGGAAAGTGGACAAGTTCCTGTATATGCTGTCACTTTTCAAGAACCTGAGAATGATCCTCGG AATTGCTGTTACTTGTGGGCTGTTCAGTCTACACAAGATAGTGAAGGGGATGTTTTGAGTTTGCATCTGC TGCAGCTGGCCTTTGGTAATAGAAAGTGTTTGGCATCAGGACAAATCTTATATGAGGGGTTAGAATACTG TGAAGAAAGATACACCCTGGACCTGACAGGTGGCATGTTCCCTTTGAGGGGACAGACGAGTAATACCAAA TTGTTGGGATGCCAGAGTATAGAGAAATTTCGATCTCATGGTGACAGGGAGGAAGGCGTGAATGAAGCTC TATCGCCTGACACTAGTGTTTCAGTCTTTACCTGGCAGGTGAATATATATGGACAGGGAAAGCCTTCTGT ATATTTGGGGCTTTTTGATATAAATCGTTGGTATCATGCACAAATGCCAGATTCGTTAAGGTCAGGAGAA TATCTACATAATTGCTCTTATTTTGCACTGTGGTCATTGGAGTCTGTTGTAAGTAGGACTTCTCCACATG GCATCTTGGATATATTAGTACATGAGAGAAGTTTAAATAGAGGAGTCCCTCCTTCATATCCACCTCCCGA GCAGTTTTTTAATCCAAGCACTTATAATTTTGATGCCACTTGTTTGTTAAACTCGGGAGTTGTTCATTTA ACTTGTACTGGCTTTCAGAAGGAGACTTTGACTTTTTTAAAGAAATCAGGTCCATCACTCAATGAACTCA TTCCTGATGGTTATAATCGATGTCTTGTAGCTGGCCTTCTTTCCCCAAGATTTGTTGATGTTCAGCCTTC CAGTTTAAGCCAAGAAGAACAGTTAGAAGCTATATTGTCAGCAGCAATTCAGACTAGTTCCCTGGGACTT TTGACTGGTTATATCCGAAGATGGATAACAGAAGAACAACCAAATTCTGCCACTAATTTGCGCTTTGTTC TTGAATGGACGTGGAATAAAGTGGTTCTCACAAAAGAGGAATTTGACAGACTATGTGTGCCATTATTTGA TGGTTCGTGTCATTTCATGGATCCACAAACTATACAGTCTATCCAGCAATGCTATTTGCTTCTTAGCAAT CTTAATATAGTCTTGAGCTGTTTTGCATCAGAAGCCCGAGAGATCACTGAGAGAGGACTGATAGACTTAA GCAATAAGTTTGTGGTTTCCCACCTCATCTGTCAGTATGCACAAGTGGTTCTTTGGTTCTCTCATTCTGG GCTTTTACCAGAAGGCATAGATGATTCTGTGCAGTTGTCAAGGTTATGCTACAACTACCCTGTAATTCAG AACTACTACACCAGTCGTCGACAGAAGTTTGAGCGTTTATCAAGAGGGAAGTGGAATCCCGATTGCTTGA TGATTGATGGACTGGTTTCTCAGTTAGGAGAGCGAATTGAGAAGTTGTGGAAACGAGATGAAGGAGGCAC AGGAAAATATCCTCCTGCTAGTCTGCATGCAGTACTTGATATGTACCTATTAGACGGCGTTACTGAAGCA GCCAAACACTCTATTACCATTTATTTGCTACTTGATATTATGTATTCCTTTCCCAACAAAACAGACACTC CCATTGAATCTTTCCCAACTGTATTTGCCATTTCTTGGGGCCAAGTTAAACTTATTCAGGGGTTTTGGTT GATAGATCATAATGACTATGAGAGTGGTTTGGATCTTTTGTTTCATCCAGCTACTGCAAAACCTTTGTCA TGGCAACATTCAAAGATTATTCAGGCATTCATGAGTCAGGGCGAGCACAGACAAGCCCTCAGATATATTC AGACAATGAAGCCAACAGTGTCCAGTGGTAACGATGTTATCCTTCACCTCACTGTTTTGCTTTTTAATAG GTGTATGGTTGAAGCCTGGAATTTTTTGCGGCAACATTGCAATAGGTTGAATATAGAGGAGTTACTGAAG CACATGTATGAAGTCTGTCAGGAAATGGGCTTGATGGAAGATTTACTGAAGTTACCATTTACAGACACTG AGCAGGAATGTTTAGTGAAATTTTTGCAGTCCAGTGCCAGCGTTCAGAATCATGAATTCCTTTTAGTGCA CCATTTGCAGCGTGCCAATTATGTGCCTGCCTTGAAGCTGAACCAAACTCTGAAGATTAATGTTATGAAT GATCGTGATCCTCGTTTGCGGGAGAGATCACTGGCTCGAAATTCTATATTAGACCAGTATGGAAAAATCC TTCCTAGAGTCCATCGAAAATTAGCCATTGAACGAGCTAAGCCTTATCATCTGTCAACATCATCAGTTTT TCGATTAGTTTCTAGACCCAAACCATTATCAGCAGTTCCAAAGCAAGTTGTAACAGGAACTGTGTTGACA AGATCTGTTTTCATCAACAATGTGTTATCTAAAATTGGAGAAGTTTGGGCAAGCAAAGAACCTATAAATA GCACCACACCTTTCAATAGTTCTAAAATAGAAGAACCATCTCCTATAGTGTATTCGCTCCCAGCTCCAGA GCTGCCTGAGGCATTTTTTGGAACACCAATTTCAAAAGCATCACAAAAAATTTCTAGACTGCTAGATTTG GTTGTTCAGCCTGTCCCCCGGCCTTCTCAGTGTTCGGAGTTTATTCAGCAAAGCTCCATGAAATCTCCTT TGTACCTAGTATCCCGTTCACTGCCCTCAAGTTCGCAATTAAAAGGATCGCCTCAGGCCATCTCCAGGGC TTCAGAATTACATTTGCTTGAAACTCCTCTTGTAGTTAAGAAAGCTAAAAGTTTGGCCATGTCAGTTACT ACTTCTGGATTTTCTGAGTTCACTCCTCAGTCCATCCTGAGGTCTACTCTTCGATCAACACCTTTAGCAT CTCCCTCTCCATCACCTGGAAGGTCTCCTCAACGACTTAAAGAAACTAGAATTTCATTTGTGGAAGAAGA TGTCCACCCAAAATGGATTCCTGGGGCTGCAGATGATAGCAAATTAGAAGTATTTACTACACCTAAAAAA TGTGCAGTTCCAGTGGAAACTGAATGGCTGAAGAGCAAAGATAGGACCACATCTTTTTTCCTGAACAGCC CTGAAAAGGAGCATCAAGAAATGGATGAGGGGTCACAAAGTTTAGAGAAACTGGATGTGAGCAAAGGAAA CAGCAGTGTTTCAATCACATCCGATGAGACTACCTTAGAGTATCAGGATGCACCGTCACCGGAAGACCTT GAAGAGACTGTTTTCACGGCCTCTAAGCCCAAAAGCTCTTCCACTGCACTAACTACTAATGTAACTGAAC AAACTGAAAAGGATGGAGATAAAGATGTATTTGCATCAGAAGTAACTCCTTCAGACCTACAGAAACAAAT GGGCAATTTAGAAGATGCAGAAACAAAGGATCTCTTAGTTGCAGCAGAGGCATTTTCAGAATTGAATCAC TTAAGCCCGGTTCAAGGAACTGAAGCTTCTCTTTGTGCACCATCAGTCTATGAAGGGAAAATCTTCACCC AGAAGTCCAAGGTACCAGTGTTGGACGAAGGATTAACATCTGTTGAAACCTACACCCCTGCAATTAGAGC AAATGACAATAAATCTATGGCTGATGTCCTTGGTGATGGTGGAAACTCCTCGCTCACTATCTCTGAAGGT CCTATTGTCTCTGAGCGCAGGCTTAACCAGGAAGTAGCGCTGAACTTAAAAGAAGATCATGAAGTAGAAG TTGGTGTACTAAAAGAAAGTGTTGACTTACCAGAAGAAAAGCTTCCAATTTCTGACAGCCCTCCTGATAC TCAAGAAATTCATGTGATTGAACAAGAAAAGCTTGAAGCTCAAGATTCAGGAGAAGAGGCTAGGAATCTT TCATTTAATGAGTTATATCCCTCTGGAACACTTAAGCTTCAGTACAATTTTGATACTATTGACCAACAGT TTTGTGACTTAGCTGATAACAAAGACACTGCTGAATGTGACATTGCTGAAGTAGATGGGGAACTTTTTGT GGCTCAAAGCAACTTTACCTTGATATTGGAAGGTGAAGAAGGAGAAGTTGAGCCAGGTGATTTTGCATCA TCTGATGTGTTACCTAAAGCAGCTAACACAGCAACTGAAGAAAAACTTGTATGCAGTGGGGAAAATGATA ATCATGGACAAATTGCAAATTTGCCATCTGCCGTAACTAGTGACCAAAAGTCCCAAAAAGTAGACACTTT ACCATATGTGCCTGAACCTATTAAAGTAGCAATTGCAGAAAATTTACTAGATGTAATTAAAGACACAAGA AGTAAAGAAATTACTTCAGATACAATGGAACAGTCCATTCATGAAACAATACCTTTAGTGAGCCAAAACA TAATGTGTCCCACTAAATTGGTCAAATCTGCATTTAAGACTGCTCAGGAAACAAGCACAATGACTATGAA TGTCAGCCAGGTTGATGACGTGGTTTCCTCCAAAACTCGTACGAGAGGTCAACGTATCCAAAACGTGAAT GTCAAATCAGCACAACAGGAAGCATCAGCAGATGTTGCTACTCCTAAGATGCCAGGGCAGTCAGTCAGGA AGAAAACTAGGAAGGCAAAAGAAATTTCTGAAGCTTCTGAAAACATCTATTCTGATGTCAGAGGACTATC TCAGAACCAGCAAATACCTCAAAATTCTGTTACGCCTAGGAGAGGAAGGAGAAAGAAAGAAGTTAATCAG GACATACTAGAAAACACCAGTTCTGTGGAACAAGAATTACAGATCACTACAGGTAGGGAATCAAAAAGAT TAAAATCATCTCAGCTGTTGGAACCAGCAGTTGAAGAAACTACTAAAAAAGAAGTTAAGGTTTCATCTGT TACAAAAAGGACTCCTAGAAGAATTAAAAGATCTGTAGAAAATCAGGAAAGTGTTGAAATTATAAATGAT CTAAAAGTTAGTACGGTAACAAGTCCTAGCAGAATGATCAGAAAATTGAGAAGTACTAATTTAGATGCTT CTGAAAATACAGGAAATAAGCAAGATGATAAATCCAGTGACAAGCAGCTGCGTATTAAACATGTTAGAAG GGTCAGAGGGAGAGAAGTTAGTCCATCAGATGTGAGAGAAGACTCCAACCTTGAGTCATCTCAGTTGACT GTTCAAGCAGAATTTGATATGTCTGCCATACCTAGAAAACGTGGTAGACCAAGAAAAATCAATCCATCTG AAGATGTAGGATCTAAGGCTGTTAAGGAAGAGAGAAGCCCCAAGAAGAAAGAAGCTCCCAGCATTAGAAG GAGATCTACAAGAAATACCCCAGCTAAAAGTGAAAATGTTGATGTTGGAAAACCAGCTTTAGGAAAATCC ATTTTAGTGCCAAACGAGGAACTTTCGATGGTGATGAGCTCTAAGAAAAAACTTACAAAAAAGACTGAAA GTCAAAGCCAAAAACGTTCATTGCACTCAGTATCAGAAGAACGCACAGATGAAATGACACATAAAGAAAC AAATGAGCAGGAAGAAAGATTGCTCGCCACAGCTTCCTTCACTAAATCATCCCGCAGCAGCAGGACTCGG TCTAGCAAGGCCATCTTGTTGCCGGACCTTTCTGAACCAAACAATGAGCCTTTATTTTCTCCAGCGTCAG AAGTTCCAAGGAAAGCAAAAGCTAAAAAAATAGAGGTTCCTGCACAGCTGAAAGAATTAGTTTCGGATTT ATCTTCTCAGTTTGTCATCTCACCTCCTGCTTTAAGGAGCAGACAAAAAAACACATCCAATAAGAACAAG CTTGAAGATGAACTGAAAGATGATGCACAATCAGTAGAAACTCTGGGAAAGCCAAAAGCGAAACGAATCA GGACGTCAAAAACAAAACAAGCAAGCAAAAACACAGAAAAAGAAAGTGCTTGGTCACCTCCTCCCATAGA AATTCGGCTGATTTCCCCCTTGGCTAGCCCAGCTGACGGAGTCAAGAGCAAACCAAGAAAAACTACAGAA GTGACAGGAACAGGTCTTGGAAGGAACAGAAAGAAACTGTCTTCCTATCCAAAGCAAATTTTACGCAGAA AAATGCTG ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA >RG215742 representing NM_015446
Red=Cloning site Green=Tags(s) MRDLRAQVTSGLLPFPEVTLQALGEDEITLESVLRGKFAAGKNGLACLACGPQLEVVNSITGERLSAYRF SGVNEQPPVVLAVKEFSWQKRTGLLIGLEETEGSVLCLYDLGISKVVKAVVLPGRVTAIEPIINHGGASA STQHLHPSLRWLFGVAAVVTDVGQILLVDLCLDDLSCNQNEVEASDLEVLTGIPAEVPHIRESVMRQGRH LCFQLVSPTGTAVSTLSYISRTNQLAVGFSDGYLALWNMKSMKREYYIQLESGQVPVYAVTFQEPENDPR NCCYLWAVQSTQDSEGDVLSLHLLQLAFGNRKCLASGQILYEGLEYCEERYTLDLTGGMFPLRGQTSNTK LLGCQSIEKFRSHGDREEGVNEALSPDTSVSVFTWQVNIYGQGKPSVYLGLFDINRWYHAQMPDSLRSGE YLHNCSYFALWSLESVVSRTSPHGILDILVHERSLNRGVPPSYPPPEQFFNPSTYNFDATCLLNSGVVHL TCTGFQKETLTFLKKSGPSLNELIPDGYNRCLVAGLLSPRFVDVQPSSLSQEEQLEAILSAAIQTSSLGL LTGYIRRWITEEQPNSATNLRFVLEWTWNKVVLTKEEFDRLCVPLFDGSCHFMDPQTIQSIQQCYLLLSN LNIVLSCFASEAREITERGLIDLSNKFVVSHLICQYAQVVLWFSHSGLLPEGIDDSVQLSRLCYNYPVIQ NYYTSRRQKFERLSRGKWNPDCLMIDGLVSQLGERIEKLWKRDEGGTGKYPPASLHAVLDMYLLDGVTEA AKHSITIYLLLDIMYSFPNKTDTPIESFPTVFAISWGQVKLIQGFWLIDHNDYESGLDLLFHPATAKPLS WQHSKIIQAFMSQGEHRQALRYIQTMKPTVSSGNDVILHLTVLLFNRCMVEAWNFLRQHCNRLNIEELLK HMYEVCQEMGLMEDLLKLPFTDTEQECLVKFLQSSASVQNHEFLLVHHLQRANYVPALKLNQTLKINVMN DRDPRLRERSLARNSILDQYGKILPRVHRKLAIERAKPYHLSTSSVFRLVSRPKPLSAVPKQVVTGTVLT RSVFINNVLSKIGEVWASKEPINSTTPFNSSKIEEPSPIVYSLPAPELPEAFFGTPISKASQKISRLLDL VVQPVPRPSQCSEFIQQSSMKSPLYLVSRSLPSSSQLKGSPQAISRASELHLLETPLVVKKAKSLAMSVT TSGFSEFTPQSILRSTLRSTPLASPSPSPGRSPQRLKETRISFVEEDVHPKWIPGAADDSKLEVFTTPKK CAVPVETEWLKSKDRTTSFFLNSPEKEHQEMDEGSQSLEKLDVSKGNSSVSITSDETTLEYQDAPSPEDL EETVFTASKPKSSSTALTTNVTEQTEKDGDKDVFASEVTPSDLQKQMGNLEDAETKDLLVAAEAFSELNH LSPVQGTEASLCAPSVYEGKIFTQKSKVPVLDEGLTSVETYTPAIRANDNKSMADVLGDGGNSSLTISEG PIVSERRLNQEVALNLKEDHEVEVGVLKESVDLPEEKLPISDSPPDTQEIHVIEQEKLEAQDSGEEARNL SFNELYPSGTLKLQYNFDTIDQQFCDLADNKDTAECDIAEVDGELFVAQSNFTLILEGEEGEVEPGDFAS SDVLPKAANTATEEKLVCSGENDNHGQIANLPSAVTSDQKSQKVDTLPYVPEPIKVAIAENLLDVIKDTR SKEITSDTMEQSIHETIPLVSQNIMCPTKLVKSAFKTAQETSTMTMNVSQVDDVVSSKTRTRGQRIQNVN VKSAQQEASADVATPKMPGQSVRKKTRKAKEISEASENIYSDVRGLSQNQQIPQNSVTPRRGRRKKEVNQ DILENTSSVEQELQITTGRESKRLKSSQLLEPAVEETTKKEVKVSSVTKRTPRRIKRSVENQESVEIIND LKVSTVTSPSRMIRKLRSTNLDASENTGNKQDDKSSDKQLRIKHVRRVRGREVSPSDVREDSNLESSQLT VQAEFDMSAIPRKRGRPRKINPSEDVGSKAVKEERSPKKKEAPSIRRRSTRNTPAKSENVDVGKPALGKS ILVPNEELSMVMSSKKKLTKKTESQSQKRSLHSVSEERTDEMTHKETNEQEERLLATASFTKSSRSSRTR SSKAILLPDLSEPNNEPLFSPASEVPRKAKAKKIEVPAQLKELVSDLSSQFVISPPALRSRQKNTSNKNK LEDELKDDAQSVETLGKPKAKRIRTSKTKQASKNTEKESAWSPPPIEIRLISPLASPADGVKSKPRKTTE VTGTGLGRNRKKLSSYPKQILRRKML TRTRPLE - GFP Tag - V |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_015446 |
| ORF Size | 6798 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_015446.3, NP_056261.3 |
| RefSeq Size | 8526 bp |
| RefSeq ORF | 6828 bp |
| Locus ID | 25909 |
| UniProt ID | Q8WYP5 |
| Domains | AT_hook |
| Protein Families | ES Cell Differentiation/IPS |
| Gene Summary | Required for the assembly of a functional nuclear pore complex (NPC) on the surface of chromosomes as nuclei form at the end of mitosis. May initiate NPC assembly by binding to chromatin and recruiting the Nup107-160 subcomplex of the NPC. Also required for the localization of the Nup107-160 subcomplex of the NPC to the kinetochore during mitosis and for the completion of cytokinesis.[UniProtKB/Swiss-Prot Function] |
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