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EPT1 (SELENOI) (NM_033505) Human Tagged ORF Clone
CAT＃: RG216434
- TrueORF®
EPT1 (GFP-tagged) - Human ethanolaminephosphotransferase 1 (CDP-ethanolamine-specific) (EPT1), (10ug), (Note: selenocysteine protein, Internal stop codon present. see reference data summary below)
ORF Plasmid: DDK
"NM_033505" in other vectors (3)
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CNY 4,370.00
Product images
Specifications
| Product Data | |
| Type | Human Untagged ORF Clone |
| Synonyms | EPT1; SELI; SEPI; SPG81 |
| Vector | pCMV6-AC-GFP |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>RG216434 representing NM_033505
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGCTGGCTACGAATACGTGAGCCCGGAGCAGCTGGCTGGCTTTGATAAGTACAAGTACAGTGCTGTGG ATACCAATCCACTTTCTCTGTATGTCATGCATCCATTCTGGAACACTATAGTAAAGGTATTTCCTACTTG GCTGGCGCCCAATCTGATAACTTTTTCTGGCTTTCTGCTGGTCGTATTCAATTTTCTGCTAATGGCATAC TTTGATCCTGACTTTTATGCCTCAGCACCAGGTCACAAGCACGTGCCTGACTGGGTTTGGATTGTAGTGG GCATCCTCAACTTCGTAGCCTACACTCTAGATGGTGTGGACGGAAAGCAAGCTCGCAGAACCAATTCTAG CACTCCCTTAGGGGAGCTTTTTGATCATGGCCTGGATAGTTGGTCATGTGTTTACTTTGTTGTGACTGTT TATTCCATCTTTGGAAGAGGATCAACTGGTGTCAGTGTTTTTGTTCTTTATCTCCTGCTATGGGTAGTTT TGTTTTCTTTCATCCTGTCCCACTGGGAAAAGTATAACACAGGGATTCTTTTCCTGCCATGGGGATATGA CATTAGCCAGGTGACTATTTCTTTTGTCTACATAGTGACTGCAGTTGTGGGAGTTGAGGCCTGGTATGAA CCTTTCCTGTTTAATTTCTTATATAGAGACCTATTCACTGCAATGATTATTGGTTGTGCATTATGTGTGA CTCTTCCAATGAGTTTATTAAACTTTTTCAGAAGCTATAAAAATAACACCTTGAAACTCAATTCAGTCTA TGAAGCTATGGTTCCCTTATTTTCTCCATGCTTGCTGTTCATTTTGTCTACAGCGTGGATCCTTTGGTCA CCTTCAGATATTTTAGAGCTACATCCTAGAGTATTCTACTTTATGGTTGGAACAGCTTTTGCCAACAGTA CATGTCAGCTGATTGTTTGCCAAATGAGTAGTACCCGGTGTCCAACTTTGAATTGGTTGCTGGTTCCTCT CTTCTTGGTTGTCTTAGTGGTAAACCTAGGAGTAGCCTCTTACGTTGAGAGCATTCTCCTGTATACATTA ACAACTGCTTTTACTCTGGCCCACATCCATTATGGAGTACGAGTGGTAAAGCAGCTGAGCAGCCATTTTC AGATTTACCCCTTCTCATTGAGGAAACCAAACTCAGATTGACTAGGAATGGAAGAAAAGAATATTGGCCT G ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA >RG216434 representing NM_033505
Red=Cloning site Green=Tags(s) MAGYEYVSPEQLAGFDKYKYSAVDTNPLSLYVMHPFWNTIVKVFPTWLAPNLITFSGFLLVVFNFLLMAY FDPDFYASAPGHKHVPDWVWIVVGILNFVAYTLDGVDGKQARRTNSSTPLGELFDHGLDSWSCVYFVVTV YSIFGRGSTGVSVFVLYLLLWVVLFSFILSHWEKYNTGILFLPWGYDISQVTISFVYIVTAVVGVEAWYE PFLFNFLYRDLFTAMIIGCALCVTLPMSLLNFFRSYKNNTLKLNSVYEAMVPLFSPCLLFILSTAWILWS PSDILELHPRVFYFMVGTAFANSTCQLIVCQMSSTRCPTLNWLLVPLFLVVLVVNLGVASYVESILLYTL TTAFTLAHIHYGVRVVKQLSSHFQIYPFSLRKPNSD*LGMEEKNIGL TRTRPLE - GFP Tag - V |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_033505 |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info The expression of this clone is not guaranteed due to the nature of selenoproteins. |
| OTI Annotation | This clone encodes a selenoprotein containing the rare amino acid selenocysteine (Sec). Sec is encoded by UGA codon, which normally signals translational termination. Expression of this clone is not guaranteed due to the nature of selenoproteins. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_033505.4 |
| RefSeq Size | 8091 bp |
| RefSeq ORF | 1194 bp |
| Locus ID | 85465 |
| UniProt ID | Q9C0D9 |
| Gene Summary | The multi-pass transmembrane protein encoded by this gene belongs to the CDP-alcohol phosphatidyltransferase class-I family. It catalyzes the transfer of phosphoethanolamine from CDP-ethanolamine to diacylglycerol to produce phosphatidylethanolamine, which is involved in the formation and maintenance of vesicular membranes, regulation of lipid metabolism, and protein folding. This protein is a selenoprotein, containing the rare selenocysteine (Sec) amino acid at its active site. Sec is encoded by the UGA codon, which normally signals translation termination. The 3' UTRs of selenoprotein mRNAs contain a conserved stem-loop structure, designated the Sec insertion sequence (SECIS) element, that is necessary for the recognition of UGA as a Sec codon rather than as a stop signal. Alternatively spliced transcript variants have been found for this gene. [provided by RefSeq, Jul 2016] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC216434 | EPT1 (Myc-DDK-tagged)-Human ethanolaminephosphotransferase 1 (CDP-ethanolamine-specific) (EPT1), (Note, selenocysteine protein, internal stop codon, see reference data summary) |
CNY 3,656.00 |
|
| RC216434L1 | Lenti-ORF, EPT1 (Myc-DDK-tagged)-Human ethanolaminephosphotransferase 1 (CDP-ethanolamine-specific) (EPT1), (Note, selenocystein protein, internal stop codon, see summary) |
CNY 6,056.00 |
|
| SC305647 | EPT1 (untagged)-Human ethanolaminephosphotransferase 1 (CDP-ethanolamine-specific) (EPT1) (10ug), (Note: selenocysteine protein, Internal stop codon present. see reference data summary below) |
CNY 6,460.00 |
