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Deformed Epidermal Autoregulatory Factor 1 (DEAF1) (NM_021008) Human Tagged ORF Clone
CAT＃: RG216583
- TrueORF®
DEAF1 (tGFP-tagged) - Human deformed epidermal autoregulatory factor 1 (Drosophila) (DEAF1)
ORF Plasmid: DDK
"NM_021008" in other vectors (4)
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CNY 5,040.00
Product images
Specifications
| Product Data | |
| Type | Human Tagged ORF Clone |
| Tag | TurboGFP |
| Synonyms | MRD24; NEDHELS; NUDR; SPN; VSVS; ZMYND5 |
| Vector | pCMV6-AC-GFP |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>RG216583 representing NM_021008
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGAGGACTCGGACTCGGCGGCAAAGCAGCTGGGCCTGGCTGAGGCGGCGGCGGTGGCGGCCGCGGCCG CTGTGGCGGCGGCGGCCGCGGCCGCGGCAGGAGGCGAGGCGGAGGAGCCGGTGCTGAGCAGGGACGAGGA CTCGGAGGAGGACGCAGACTCGGAGGCGGAGCGGGAGACGCCGCGGGTCACGGCAGTGGCGGTGATGGCG GCGGAGCCCGGGCACATGGACATGGGCGCCGAGGCCCTGCCCGGCCCCGACGAGGCCGCCGCTGCCGCAG CCTTCGCAGAGGTGACCACAGTGACAGTGGCCAACGTGGGGGCTGCTGCAGACAATGTCTTCACCACGTC TGTGGCGAACGCGGCATCCATCTCAGGACATGTTCTGTCTGGTAGGACGGCCCTTCAGATCGGGGACAGC CTGAACACCGAAAAAGCGACACTGATTGTCGTCCACACAGATGGGAGCATCGTGGAGACCACCGGGCTGA AAGGCCCGGCAGCTCCCCTCACCCCAGGTCCTCAGTCTCCTCCAACCCCTCTGGCTCCCGGCCAAGAAAA AGGTGGAACTAAATACAACTGGGACCCTTCTGTGTACGACAGTGAGCTGCCCGTACGGTGCCGGAACATC AGCGGCACTCTGTACAAGAACAGGCTCGGCTCAGGCGGCCGGGGACGGTGCATCAAGCAGGGGGAGAACT GGTACAGTCCCACCGAGTTTGAGGCCATGGCAGGAAGAGCCAGCAGTAAGGACTGGAAAAGAAGCATTCG CTACGCGGGCCGACCCTTGCAGTGCCTCATCCAGGATGGGATCTTAAACCCTCACGCTGCCTCTTGCACC TGTGCTGCCTGCTGCGACGACATGACCTTAAGTGGCCCAGTCAGGCTTTTTGTGCCTTACAAAAGGCGCA AGAAGGAGAATGAACTGCCCACAACTCCCGTGAAGAAGGACTCCCCCAAGAACATCACATTGCTTCCAGC CACCGCGGCTACCACCTTCACCGTGACCCCCTCGGGACAGATCACGACCTCGGGGGCACTGACCTTTGAC CGAGCGTCCACGGTAGAGGCCACTGCTGTCATATCAGAGAGTCCGGCCCAGGGCGACGTCTTCGCAGGGG CCACAGTCCAAGAGGCCAGCGTGCAGCCCCCATGCAGGGCCAGCCACCCTGAGCCTCACTACCCCGGCTA TCAGGACAGCTGCCAGATCGCACCGTTTCCAGAAGCTGCGTTGCCAACGTCACATCCCAAAATAGTGTTG ACATCCCTGCCTGCGCTGGCGGTCCCACCCCCGACTCCCACCAAAGCGGCACCTCCCGCGTTGGTCAATG GGCTGGAGCTGTCAGAGCCGCGGAGCTGGCTGTACCTAGAAGAGATGGTCAACTCCTTGCTCAACACAGC GCAGCAGCTGAAGACGCTGTTTGAGCAAGCCAAGCATGCCAGCACCTACCGAGAAGCTGCCACAAACCAG GCCAAGATCCACGCTGACGCAGAGCGGAAGGAGCAGTCCTGCGTTAACTGCGGCCGGGAGGCTATGAGCG AGTGCACCGGCTGCCACAAGGTCAACTACTGCTCCACCTTCTGCCAACGCAAGGACTGGAAGGATCACCA GCACATATGCGGCCAGTCAGCAGCTGTCACCGTCCAGGCAGACGAAGTCCACGTGGCTGAAAGCGTGATG GAGAAGGTGACCGTG ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA >RG216583 representing NM_021008
Red=Cloning site Green=Tags(s) MEDSDSAAKQLGLAEAAAVAAAAAVAAAAAAAAGGEAEEPVLSRDEDSEEDADSEAERETPRVTAVAVMA AEPGHMDMGAEALPGPDEAAAAAAFAEVTTVTVANVGAAADNVFTTSVANAASISGHVLSGRTALQIGDS LNTEKATLIVVHTDGSIVETTGLKGPAAPLTPGPQSPPTPLAPGQEKGGTKYNWDPSVYDSELPVRCRNI SGTLYKNRLGSGGRGRCIKQGENWYSPTEFEAMAGRASSKDWKRSIRYAGRPLQCLIQDGILNPHAASCT CAACCDDMTLSGPVRLFVPYKRRKKENELPTTPVKKDSPKNITLLPATAATTFTVTPSGQITTSGALTFD RASTVEATAVISESPAQGDVFAGATVQEASVQPPCRASHPEPHYPGYQDSCQIAPFPEAALPTSHPKIVL TSLPALAVPPPTPTKAAPPALVNGLELSEPRSWLYLEEMVNSLLNTAQQLKTLFEQAKHASTYREAATNQ AKIHADAERKEQSCVNCGREAMSECTGCHKVNYCSTFCQRKDWKDHQHICGQSAAVTVQADEVHVAESVM EKVTV TRTRPLE - GFP Tag - V |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_021008 |
| ORF Size | 1695 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_021008.4 |
| RefSeq Size | 2737 bp |
| RefSeq ORF | 1698 bp |
| Locus ID | 10522 |
| UniProt ID | O75398 |
| Domains | SAND, zf-MYND |
| Protein Families | Secreted Protein, Transcription Factors, Transmembrane |
| Gene Summary | This gene encodes a zinc finger domain-containing protein that functions as a regulator of transcription. The encoded proteins binds to its own promoter as well as to that of several target genes. Activity of this protein is important in the regulation of embryonic development. Mutations in this gene have been found in individuals with autosomal dominant cognitive disability. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Jun 2014] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC216583 | DEAF1 (Myc-DDK-tagged)-Human deformed epidermal autoregulatory factor 1 (Drosophila) (DEAF1) |
CNY 4,208.00 |
|
| RC216583L3 | Lenti-ORF clone of DEAF1 (Myc-DDK-tagged)-Human deformed epidermal autoregulatory factor 1 (Drosophila) (DEAF1) |
CNY 6,460.00 |
|
| RC216583L4 | Lenti-ORF clone of DEAF1 (mGFP-tagged)-Human deformed epidermal autoregulatory factor 1 (Drosophila) (DEAF1) |
CNY 6,460.00 |
|
| SC108819 | DEAF1 (untagged)-Human deformed epidermal autoregulatory factor 1 (Drosophila) (DEAF1) |
CNY 4,216.00 |
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