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AKAP4 (NM_139289) Human Tagged ORF Clone
CAT＃: RG217793
- TrueORF®
AKAP4 (tGFP-tagged) - Human A kinase (PRKA) anchor protein 4 (AKAP4), transcript variant 2
ORF Plasmid: DDK
"NM_139289" in other vectors (4)
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CNY 9,690.00
Product images
Specifications
| Product Data | |
| Type | Human Tagged ORF Clone |
| Tag | TurboGFP |
| Synonyms | AKAP-4; AKAP 82; AKAP82; CT99; FSC1; hAKAP82; HI; p82; PRKA4 |
| Vector | pCMV6-AC-GFP |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>RG217793 representing NM_139289
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGTCTGATGATATTGACTGGTTACGCAGCCACAGGGGTGTGTGCAAGGTAGATCTCTACAACCCAGAAG GACAGCAAGATCAGGACCGGAAAGTGATATGCTTTGTCGATGTGTCCACCCTGAATGTAGAAGATAAAGA TTACAAGGATGCTGCTAGTTCCAGCTCAGAAGGCAACTTAAACCTGGGAAGTCTGGAAGAAAAAGAGATT ATCGTGATCAAGGACACTGAGAAGAAAGACCAGTCTAAGACAGAGGGATCTGTATGCCTTTTCAAACAAG CTCCCTCTGATCCTGTAAGTGTCCTCAACTGGCTTCTCAGTGATCTCCAGAAGTATGCCTTGGGTTTCCA ACATGCACTGAGCCCCTCAACCTCTACCTGTAAACATAAAGTAGGAGACACAGAGGGCGAATATCACAGA GCATCCTCTGAGAACTGCTACAGTGTCTATGCCGATCAAGTGAACATAGATTATTTGATGAACAGACCTC AAAACCTACGTCTAGAAATGACAGCAGCTAAAAACACCAACAATAATCAAAGTCCTTCAGCTCCTCCAGC CAAACCTCCTAGCACTCAGAGAGCAGTCATTTCCCCTGATGGAGAATGTTCTATAGATGACCTTTCCTTC TACGTCAACCGACTATCTTCTCTGGTAATCCAGATGGCCCATAAGGAAATCAAGGAGAAGTTGGAAGGTA AAAGCAAATGCCTTCATCATTCAATCTGTCCATCCCCTGGGAACAAAGAGAGAATCAGTCCCCGAACTCC TGCGAGCAAGATTGCTTCTGAAATGGCCTATGAAGCTGTGGAACTGACAGCTGCAGAAATGCGTGGCACT GGAGAGGAGTCCAGGGAAGGTGGCCAGAAAAGCTTTCTATATAGCGAATTATCCAACAAGAGCAAAAGTG GAGACAAACAGATGTCCCAGAGAGAGAGCAAAGAATTTGCAGATTCCATCAGCAAGGGGCTCATGGTTTA TGCAAATCAGGTGGCATCTGACATGATGGTCTCTCTCATGAAGACCTTGAAAGTGCACAGCTCTGGGAAG CCAATTCCAGCATCTGTGGTCCTGAAGAGGGTGTTGCTAAGGCACACCAAGGAGATTGTGTCCGATTTGA TTGATTCTTGCATGAAGAACCTGCATAATATTACTGGGGTCCTGATGACTGACTCAGACTTTGTCTCAGC TGTCAAGAGAAATCTGTTCAACCAGTGGAAACAAAATGCTACAGACATCATGGAGGCCATGCTGAAGCGC TTGGTCAGTGCCCTTATAGGTGAGGAGAAGGAGACTAAGTCTCAGAGTCTGTCATATGCATCTTTAAAAG CTGGGTCCCATGATCCCAAATGCAGGAATCAGAGTCTTGAATTCTCCACCATGAAAGCTGAAATGAAAGA GAGGGACAAAGGCAAAATGAAATCAGACCCATGCAAGTCACTGACTAGTGCTGAGAAAGTCGGTGAACAC ATTCTCAAAGAGGGCCTAACCATCTGGAACCAAAAGCAAGGAAACTCATGCAAGGTGGCTACCAAAGCAT GCAGCAATAAAGATGAGAAAGGAGAAAAGATCAATGCTTCCACAGATTCACTGGCCAAGGACCTGATTGT CTCTGCCCTTAAGCTGATCCAGTACCATCTGACCCAGCAGACTAAGGGCAAAGATACATGTGAAGAAGAC TGTCCTGGTTCCACCATGGGCTATATGGCTCAGAGTACTCAATATGAAAAGTGTGGAGGTGGCCAAAGTG CCAAAGCACTTTCAGTGAAACAACTAGAATCTCACAGAGCCCCTGGACCATCCACCTGTCAAAAGGAGAA CCAACACCTGGACTCCCAGAAAATGGATATGTCAAACATCGTTCTAATGCTGATTCAGAAACTGCTTAAT GAGAACCCCTTCAAATGTGAGGATCCATGCGAAGGTGAGAACAAGTGTTCTGAGCCCAGGGCAAGCAAAG CAGCTTCCATGTCCAACAGATCTGACAAAGCGGAAGAACAATGCCAGGAGCATCAAGAACTTGACTGTAC CAGTGGGATGAAGCAAGCGAACGGGCAATTTATAGATAAACTAGTAGAATCTGTGATGAAGCTCTGCCTT ATCATGGCTAAGTATAGCAACGATGGGGCAGCCCTTGCTGAGTTGGAAGAACAAGCAGCCTCGGCAAATA AGCCCAATTTCAGGGGCACCAGATGCATTCACAGTGGTGCAATGCCACAGAACTATCAAGACTCTCTTGG ACATGAAGTAATTGTCAATAATCAGTGCTCTACAAATAGCTTGCAGAAGCAGCTCCAGGCTGTCCTGCAG TGGATTGCAGCCTCCCAGTTTAACGTGCCCATGCTCTACTTCATGGGAGATAAGGATGGACAACTGGAAA AGCTTCCTCAGGTTTCAGCTAAAGCAGCAGAGAAGGGGTACAGTGTAGGAGGTCTTCTTCAAGAGGTCAT GAAGTTTGCCAAGGAACGGCAACCAGATGAAGCTGTGGGAAAGGTGGCCAGGAAACAGTTGCTGGACTGG CTGCTCGCTAACCTG ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA >RG217793 representing NM_139289
Red=Cloning site Green=Tags(s) MSDDIDWLRSHRGVCKVDLYNPEGQQDQDRKVICFVDVSTLNVEDKDYKDAASSSSEGNLNLGSLEEKEI IVIKDTEKKDQSKTEGSVCLFKQAPSDPVSVLNWLLSDLQKYALGFQHALSPSTSTCKHKVGDTEGEYHR ASSENCYSVYADQVNIDYLMNRPQNLRLEMTAAKNTNNNQSPSAPPAKPPSTQRAVISPDGECSIDDLSF YVNRLSSLVIQMAHKEIKEKLEGKSKCLHHSICPSPGNKERISPRTPASKIASEMAYEAVELTAAEMRGT GEESREGGQKSFLYSELSNKSKSGDKQMSQRESKEFADSISKGLMVYANQVASDMMVSLMKTLKVHSSGK PIPASVVLKRVLLRHTKEIVSDLIDSCMKNLHNITGVLMTDSDFVSAVKRNLFNQWKQNATDIMEAMLKR LVSALIGEEKETKSQSLSYASLKAGSHDPKCRNQSLEFSTMKAEMKERDKGKMKSDPCKSLTSAEKVGEH ILKEGLTIWNQKQGNSCKVATKACSNKDEKGEKINASTDSLAKDLIVSALKLIQYHLTQQTKGKDTCEED CPGSTMGYMAQSTQYEKCGGGQSAKALSVKQLESHRAPGPSTCQKENQHLDSQKMDMSNIVLMLIQKLLN ENPFKCEDPCEGENKCSEPRASKAASMSNRSDKAEEQCQEHQELDCTSGMKQANGQFIDKLVESVMKLCL IMAKYSNDGAALAELEEQAASANKPNFRGTRCIHSGAMPQNYQDSLGHEVIVNNQCSTNSLQKQLQAVLQ WIAASQFNVPMLYFMGDKDGQLEKLPQVSAKAAEKGYSVGGLLQEVMKFAKERQPDEAVGKVARKQLLDW LLANL TRTRPLE - GFP Tag - V |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_139289 |
| ORF Size | 2535 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_139289.2 |
| RefSeq Size | 2867 bp |
| RefSeq ORF | 2538 bp |
| Locus ID | 8852 |
| UniProt ID | Q5JQC9 |
| Protein Families | Druggable Genome |
| Gene Summary | The A-kinase anchor proteins (AKAPs) are a group of structurally diverse proteins, which have the common function of binding to the regulatory subunit of protein kinase A (PKA) and confining the holoenzyme to discrete locations within the cell. This gene encodes a member of the AKAP family. The encoded protein is localized to the sperm flagellum and may be involved in the regulation of sperm motility. Alternative splicing of this gene results in two transcript variants encoding different isoforms. [provided by RefSeq, Jul 2008] |
Documents
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| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC217793 | AKAP4 (Myc-DDK-tagged)-Human A kinase (PRKA) anchor protein 4 (AKAP4), transcript variant 2 |
CNY 6,192.00 |
|
| RC217793L3 | Lenti-ORF clone of AKAP4 (Myc-DDK-tagged)-Human A kinase (PRKA) anchor protein 4 (AKAP4), transcript variant 2 |
CNY 10,740.00 |
|
| RC217793L4 | Lenti-ORF clone of AKAP4 (mGFP-tagged)-Human A kinase (PRKA) anchor protein 4 (AKAP4), transcript variant 2 |
CNY 10,740.00 |
|
| SC306141 | AKAP4 (untagged)-Human A kinase (PRKA) anchor protein 4 (AKAP4), transcript variant 2 |
CNY 16,340.00 |
