ANKFN1 (NM_153228) Human Tagged ORF Clone

CAT＃: RG217952

TrueORF^®

ANKFN1 (tGFP-tagged) - Human ankyrin-repeat and fibronectin type III domain containing 1 (ANKFN1)

ORF Plasmid: DDK tGFP

Datasheet

SDS

Plasmid Map

"NM_153228" in other vectors (6)

Need custom modification / cloning service?
Get a free quote

CNY 8,930.00

货期*

3周

规格

Product images

经常一起买 (4)

PS100010

pCMV6-AC-GFP, mammalian vector with C-terminal tGFP tag, 10ug

CNY 6,080.00

TA150041

Mouse monoclonal turboGFP antibody, clone OTI2H8

CNY 1,000.00

【特别福利】购一抗正装产品，免费领10ul抗体试用装

CC100003

DH5α Chemically Competent Cells (≥10^8 cfu/μg of pUC19 DNA)

CNY 1,280.00

PRIMER

Forward sequencing primer VP1.5, Reverse sequencing primer XL39, 100pmoles each

CNY 480.00

Specifications

Product Data
Type	Human Tagged ORF Clone
Tag	TurboGFP
Vector	pCMV6-AC-GFP
E. coli Selection	Ampicillin (100 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	ORF Nucleotide Sequence (showhide) >RG217952 representing NM_153228 Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGAGGCGTCTCTAACCAGGAGGCTACTCTTTAAAGACAGGCATTTTACTTGCAGCAAAATAATAGGAA GGAGATTCGCTTGCTTTGCACAGAGGCTGAGCCACAGGAGAAAGCAAAGCCAATGTGATTTATTGAATGA AAGCACTGGACAATTACCAACAACTTGTTCCTCTGCTGCCTCGAACAGCATAAACTGGAATTGTCGTGTG AAAATGACGCAACAAATGCAAAATTTACATCTCTGTCAGTCAAAAAAACATAGTGCTCCCTCATCTCCCA ACGCAGCCAAACGCCTGTACAGGAACCTCTCTGAGAAACTGAAAGGGAGCCACTCTTCCTTCGATGAGGC CTATTTTAGGACAAGAACTGATCGGCTGAGTCTCAGGAAGACCTCGGTGAATTTCCAGGGCAATGAAGCC ATGTTTGAGGCAGTCGAACAGCAGGACATGGATGCTGTGCAGATCCTCCTGTATCAGTACACACCAGAAG AACTTGACCTCAACACACCTAACAGCGAGGGCTTGACACCCCTGGATATTGCCATCATGACCAACAATGT GCCCATTGCAAGGATTCTTCTGAGGACAGGGGCCCGAGAAAGTCCACACTTTGTCAGCCTGGAAAGCCGA GCAATGCACCTAAACACACTGGTCCAGGAAGCCCAGGAGAGGGTGAGTGAACTGTCTGCCCAGGTGGAGA ATGAAGGATTCACTCTGGACAACACAGAGAAAGAGAAACAGCTGAAAGCTTGGGAGTGGAGGTATCGGCT CTACAGACGCATGAAAACAGGCTTTGAGCATGCCAGAGCCCCTGAGATGCCAACCAATGTCTGTCTCATG GTAACCAGCAGCACATCACTCACTGTCAGCTTCCAAGAGCCTCTTAGCGTCAATGCAGCTGTAGTAACCA GGTATAAAGTGGAATGGAGTATGTCCGAAGACTTTTCTCCTTTGGCTGGAGAAATCATCATGGATAATCT GCAGACTCTGAGATGCACAATCACAGGACTTACAATGGGCCAACAGTATTTTGTTCAAGTCTCGGCTTAC AATATGAAAGGATGGGGACCTGCTCAGACCACGACACCGGCATGTGCCTCTCCTTCTAACTGGAAAGACT ATGACGACAGAGAGCCCAGACACAAGGGACAGAGTGAAGTTTTGGAAGGTCTGCTGCAGCAGGTCCGAGC CCTTCATCAGCATTACAGTTGCCGGGAAAGCACAAAATTACAAACCACAGGCCGCAAGCAGTCAGTCTCA AGAAGCCTGAAACACCTGTTCCATTCCTCGAACAAGTTTGTGAAGACCTTAAAACGGGGACTCTACATAG CCATTATATTTTATTACAAAGACAATATCTTAGTCACCAATGAAGATCAAGTACCAATTGTTGAAATAGA TGACTCTCACACCAGTTCTATTACACAAGATTTTCTGTGGTTCACGAAGCTGTCTTGTATGTGGGAAGAT ATAAGGTGGCTGAGGCAAAGCATACCAATATCCTCATCCTCATCCACAGTGCTGCAAACTCGGCAGAAGA TGCTCGCAGCAACAGCACAGCTACAGAATTTACTTGGGACACACAACTTGGGAAGAGTTTACTATGAGCC CATTAAAGATCGACATGGAAACATACTCATAGTCACCATCAGGGAGGTGGAGATGCTTTATTCATTTTTT AATGGCAAATGGATGCAGATCTCAAAGCTGCAAAGCCAGAGAAAGTCTCTATCAACACCTGAGGAGCCAA CAGCTTTAGACATTCTACTGATAACCATCCAGGATATTCTATCCTATCACAAAAGGAGTCATCAGCGTCT CTTTCCTGGATTATATCTGGGTTACCTAAAGCTCTGTAGCTCTGTGGATCAAATCAAAGTTCTTGTTACC CAAAAGTTGCCCAACATTCTCTGCCACGTGAAGATCCGTGAAAACAATAATATTTCTAGAGAGGAATGGG AATGGATCCAAAAGCTTTCTGGCTCTGAATCTATGGAAAGTGTGGATCATACTTCTGACTGCCCCATGCA ATTGTTCTTCTACGAGCTCCAGATGGCAGTGAAAGCTCTCCTTCAGCAGATCAATATACCTCTACACCAG GCAAGGAACTTCCGCCTCTACACACAGGAGGTGTTGGAAATGGGTCACAATGTGTCCTTTCTTCTCCTGC TCCCTGCCTCAGACGACGTCTGTACAGCCCCAGGACAGAATAATCCTTACACCCCACACTCAGGGTTTCT TAACCTCCCTCTTCAGATGTTTGAACTTGGTATAGTAGCTTGTTTCACC ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA Protein Sequence (showhide) >RG217952 representing NM_153228 Red=Cloning site Green=Tags(s) MEASLTRRLLFKDRHFTCSKIIGRRFACFAQRLSHRRKQSQCDLLNESTGQLPTTCSSAASNSINWNCRV KMTQQMQNLHLCQSKKHSAPSSPNAAKRLYRNLSEKLKGSHSSFDEAYFRTRTDRLSLRKTSVNFQGNEA MFEAVEQQDMDAVQILLYQYTPEELDLNTPNSEGLTPLDIAIMTNNVPIARILLRTGARESPHFVSLESR AMHLNTLVQEAQERVSELSAQVENEGFTLDNTEKEKQLKAWEWRYRLYRRMKTGFEHARAPEMPTNVCLM VTSSTSLTVSFQEPLSVNAAVVTRYKVEWSMSEDFSPLAGEIIMDNLQTLRCTITGLTMGQQYFVQVSAY NMKGWGPAQTTTPACASPSNWKDYDDREPRHKGQSEVLEGLLQQVRALHQHYSCRESTKLQTTGRKQSVS RSLKHLFHSSNKFVKTLKRGLYIAIIFYYKDNILVTNEDQVPIVEIDDSHTSSITQDFLWFTKLSCMWED IRWLRQSIPISSSSSTVLQTRQKMLAATAQLQNLLGTHNLGRVYYEPIKDRHGNILIVTIREVEMLYSFF NGKWMQISKLQSQRKSLSTPEEPTALDILLITIQDILSYHKRSHQRLFPGLYLGYLKLCSSVDQIKVLVT QKLPNILCHVKIRENNNISREEWEWIQKLSGSESMESVDHTSDCPMQLFFYELQMAVKALLQQINIPLHQ ARNFRLYTQEVLEMGHNVSFLLLLPASDDVCTAPGQNNPYTPHSGFLNLPLQMFELGIVACFT TRTRPLE - GFP Tag - V
Restriction Sites	SgfI-MluI Cloning Scheme for this gene Plasmid Map
ACCN	NM_153228
ORF Size	2289 bp
OTI Disclaimer	The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation	This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq	NM_153228.1, NP_694960.1
RefSeq Size	2426 bp
RefSeq ORF	2292 bp
Locus ID	162282
UniProt ID	Q8N957
Domains	ANK, FN3
Protein Families	Druggable Genome

*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

Documents

Product Manuals
TrueORFs: Open Reading Frame Clones - 10 ug in PrecisionShuttle Vector System pLenti-ORF destination vector Transfection - TurboFectin 8.0 Transfection Reagent Plasmid Preparation - PowerPrep Plasmid Purification Kits
FAQs
TrueORF Clones/PrecisionShuttle Organelle Marker Transfection Reagent
SDS
TrueORF SDS

Resources

cDNA 克隆相关资源
Video Tutorial: Clone Selection Guide Product Video: What is TrueORF GOLD and why it is the most popular type of clone Video Tutorial: How to make Lentivirus particles Video Tutorial: Is the Lentivirus Safe Product Brochure: cDNA clones $98 Clones. Download the List of 15,000 Protocol: Lentivirus packaging

Other Versions

SKU	Description	Size	Price
RC217952	ANKFN1 (Myc-DDK-tagged)-Human ankyrin-repeat and fibronectin type III domain containing 1 (ANKFN1)	10 ug 产品选项验证失败所有选项都是必需的	CNY 5,592.00 CNY 8,080.00
RC217952L1	Lenti ORF clone of Human ankyrin-repeat and fibronectin type III domain containing 1 (ANKFN1), Myc-DDK-tagged	10 ug 产品选项验证失败所有选项都是必需的	CNY 7,992.00 CNY 9,980.00
RC217952L2	Lenti ORF clone of Human ankyrin-repeat and fibronectin type III domain containing 1 (ANKFN1), mGFP tagged	10 ug 产品选项验证失败所有选项都是必需的	CNY 9,980.00
RC217952L3	Lenti ORF clone of Human ankyrin-repeat and fibronectin type III domain containing 1 (ANKFN1), Myc-DDK-tagged	10 ug 产品选项验证失败所有选项都是必需的	CNY 9,980.00
RC217952L4	Lenti ORF clone of Human ankyrin-repeat and fibronectin type III domain containing 1 (ANKFN1), mGFP tagged	10 ug 产品选项验证失败所有选项都是必需的	CNY 9,980.00
SC314643	ANKFN1 (untagged)-Human ankyrin-repeat and fibronectin type III domain containing 1 (ANKFN1)	10 ug 产品选项验证失败所有选项都是必需的	CNY 6,152.00

You may also need

Empty Vector

tGFP Antibody

其它ANKFN1产品

Expression Plasmids

Lentiviral Particles

CRISPR

RNAi

Proteins

Antibodies