Dopamine Transporter (SLC6A3) (NM_001044) Human Tagged ORF Clone
CAT#: RG219466
- TrueORF®
SLC6A3 (tGFP-tagged) - Human solute carrier family 6 (neurotransmitter transporter, dopamine), member 3 (SLC6A3)
ORF Plasmid: DDK
"NM_001044" in other vectors (6)
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CNY 6,216.00
Cited in 1 publication. |
Specifications
Product Data | |
Type | Human Tagged ORF Clone |
Tag | TurboGFP |
Synonyms | DAT; DAT1; PKDYS; PKDYS1 |
Vector | pCMV6-AC-GFP |
E. coli Selection | Ampicillin (100 ug/mL) |
Mammalian Cell Selection | Neomycin |
Sequence Data |
>RG219466 representing NM_001044
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGAGTAAGAGCAAATGCTCCGTGGGACTCATGTCTTCCGTGGTGGCCCCGGCTAAGGAGCCCAATGCCG TGGGCCCGAAGGAGGTGGAGCTCATCCTTGTCAAGGAGCAGAACGGAGTGCAGCTCACCAGCTCCACCCT CACCAACCCGCGGCAGAGCCCCGTGGAGGCCCAGGATCGGGAGACCTGGGGCAAGAAGATCGACTTTCTC CTGTCCGTCATTGGCTTTGCTGTGGACCTGGCCAACGTCTGGCGGTTCCCCTACCTGTGCTACAAAAATG GTGGCGGTGCCTTCCTGGTCCCCTACCTGCTCTTCATGGTCATTGCTGGGATGCCACTTTTCTACATGGA GCTGGCCCTCGGCCAGTTCAACAGGGAAGGGGCCGCTGGTGTCTGGAAGATCTGCCCCATACTGAAAGGT GTGGGCTTCACGGTCATCCTCATCTCACTGTATGTCGGCTTCTTCTACAACGTCATCATCGCCTGGGCGC TGCACTATCTCTTCTCCTCCTTCACCACGGAGCTCCCCTGGATCCACTGCAACAACTCCTGGAACAGCCC CAACTGCTCGGATGCCCATCCTGGTGACTCCAGTGGAGACAGCTCGGGCCTCAACGACACTTTTGGGACC ACACCTGCTGCCGAGTACTTTGAACGTGGCGTGCTGCACCTCCACCAGAGCCATGGCATCGACGACCTGG GGCCTCCGCGGTGGCAGCTCACAGCCTGCCTGGTGCTGGTCATCGTGCTGCTCTACTTCAGCCTCTGGAA GGGCGTGAAGACCTCAGGGAAGGTGGTATGGATCACAGCCACCATGCCATACGTGGTCCTCACTGCCCTG CTCCTGCGTGGGGTCACCCTCCCTGGAGCCATAGACGGCATCAGAGCATACCTGAGCGTTGACTTCTACC GGCTCTGCGAGGCGTCTGTTTGGATTGACGCGGCCACCCAGGTGTGCTTCTCCCTGGGCGTGGGGTTCGG GGTGCTGATCGCCTTCTCCAGCTACAACAAGTTCACCAACAACTGCTACAGGGACGCGATTGTCACCACC TCCATCAACTCCCTGACGAGCTTCTCCTCCGGCTTCGTCGTCTTCTCCTTCCTGGGGTACATGGCACAGA AGCACAGTGTGCCCATCGGGGACGTGGCCAAGGACGGGCCAGGGCTGATCTTCATCATCTACCCGGAAGC CATCGCCACGCTCCCTCTGTCCTCAGCCTGGGCCGTGGTCTTCTTCATCATGCTGCTCACCCTGGGTATC GACAGCGCCATGGGTGGTATGGAGTCAGTGATCACCGGGCTCATCGATGAGTTCCAGCTGCTGCACAGAC ACCGTGAGCTCTTCACGCTCTTCATCGTCCTGGCGACCTTCCTCCTGTCCCTGTTCTGCGTCACCAACGG TGGCATCTACGTCTTCACGCTCCTGGACCATTTTGCAGCCGGCACGTCCATCCTCTTTGGAGTGCTCATC GAAGCCATCGGAGTGGCCTGGTTCTATGGTGTTGGGCAGTTCAGCGACGACATCCAGCAGATGACCGGGC AGCGGCCCAGCCTGTACTGGCGGCTGTGCTGGAAGCTGGTCAGCCCCTGCTTTCTCCTGTTCGTGGTCGT GGTCAGCATTGTGACCTTCAGACCCCCCCACTACGGAGCCTACATCTTCCCCGACTGGGCCAACGCGCTG GGCTGGGTCATCGCCACATCCTCCATGGCCATGGTGCCCATCTATGCGGCCTACAAGTTCTGCAGCCTGC CTGGGTCCTTTCGAGAGAAACTGGCCTACGCCATTGCACCCGAGAAGGACCGTGAGCTGGTGGACAGAGG GGAGGTGCGCCAGTTCACGCTCCGCCACTGGCTCAAGGTG ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA >RG219466 representing NM_001044
Red=Cloning site Green=Tags(s) MSKSKCSVGLMSSVVAPAKEPNAVGPKEVELILVKEQNGVQLTSSTLTNPRQSPVEAQDRETWGKKIDFL LSVIGFAVDLANVWRFPYLCYKNGGGAFLVPYLLFMVIAGMPLFYMELALGQFNREGAAGVWKICPILKG VGFTVILISLYVGFFYNVIIAWALHYLFSSFTTELPWIHCNNSWNSPNCSDAHPGDSSGDSSGLNDTFGT TPAAEYFERGVLHLHQSHGIDDLGPPRWQLTACLVLVIVLLYFSLWKGVKTSGKVVWITATMPYVVLTAL LLRGVTLPGAIDGIRAYLSVDFYRLCEASVWIDAATQVCFSLGVGFGVLIAFSSYNKFTNNCYRDAIVTT SINSLTSFSSGFVVFSFLGYMAQKHSVPIGDVAKDGPGLIFIIYPEAIATLPLSSAWAVVFFIMLLTLGI DSAMGGMESVITGLIDEFQLLHRHRELFTLFIVLATFLLSLFCVTNGGIYVFTLLDHFAAGTSILFGVLI EAIGVAWFYGVGQFSDDIQQMTGQRPSLYWRLCWKLVSPCFLLFVVVVSIVTFRPPHYGAYIFPDWANAL GWVIATSSMAMVPIYAAYKFCSLPGSFREKLAYAIAPEKDRELVDRGEVRQFTLRHWLKV TRTRPLE - GFP Tag - V |
Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
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ACCN | NM_001044 |
ORF Size | 1860 bp |
OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
Reference Data | |
RefSeq | NM_001044.5 |
RefSeq Size | 3945 bp |
RefSeq ORF | 1863 bp |
Locus ID | 6531 |
UniProt ID | Q01959 |
Domains | SNF |
Protein Families | Druggable Genome, Transmembrane |
Protein Pathways | Parkinson's disease |
Gene Summary | This gene encodes a dopamine transporter which is a member of the sodium- and chloride-dependent neurotransmitter transporter family. The 3' UTR of this gene contains a 40 bp tandem repeat, referred to as a variable number tandem repeat or VNTR, which can be present in 3 to 11 copies. Variation in the number of repeats is associated with idiopathic epilepsy, attention-deficit hyperactivity disorder, dependence on alcohol and cocaine, susceptibility to Parkinson disease and protection against nicotine dependence.[provided by RefSeq, Nov 2009] |
Citations (1)
The use of this cDNA Clones has been cited in the following citations: |
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“Ecstasy” to Addiction: Mechanisms and Reinforcing Effects of Three Synthetic Cathinone Analogs of MDMA
,null,
Neuropharmacology
,PubMed ID 29378213
[SLC6A3]
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Documents
Product Manuals |
FAQs |
SDS |
Resources
Other Versions
SKU | Description | Size | Price |
---|---|---|---|
RC219466 | SLC6A3 (Myc-DDK-tagged)-Human solute carrier family 6 (neurotransmitter transporter, dopamine), member 3 (SLC6A3) |
CNY 4,616.00 |
|
RC219466L1 | Lenti ORF clone of Human solute carrier family 6 (neurotransmitter transporter, dopamine), member 3 (SLC6A3), Myc-DDK-tagged |
CNY 7,016.00 |
|
RC219466L2 | Lenti ORF clone of Human solute carrier family 6 (neurotransmitter transporter, dopamine), member 3 (SLC6A3), mGFP tagged |
CNY 6,460.00 |
|
RC219466L3 | Lenti ORF clone of Human solute carrier family 6 (neurotransmitter transporter, dopamine), member 3 (SLC6A3), Myc-DDK-tagged |
CNY 7,016.00 |
|
RC219466L4 | Lenti ORF clone of Human solute carrier family 6 (neurotransmitter transporter, dopamine), member 3 (SLC6A3), mGFP tagged |
CNY 7,016.00 |
|
SC302961 | SLC6A3 (untagged)-Human solute carrier family 6 (neurotransmitter transporter, dopamine), member 3 (SLC6A3) |
CNY 4,624.00 |
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