PDLIM2 (NM_176871) Human Tagged ORF Clone

CAT#: RG219644

  • TrueORF®

PDLIM2 (tGFP-tagged) - Human PDZ and LIM domain 2 (mystique) (PDLIM2), transcript variant 1

ORF Plasmid: DDK tGFP



  "NM_176871" in other vectors (6)

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CNY 5,256.00


货期*
现货

规格
    • 10 ug

Product images

经常一起买 (5)
pCMV6-AC-GFP, mammalian vector with C-terminal tGFP tag, 10ug
    • 10 ug

CNY 6,080.00


Mouse monoclonal turboGFP antibody, clone OTI2H8
    • 100 ul

CNY 1,000.00


DH5α Chemically Competent Cells (≥10^8 cfu/μg of pUC19 DNA)
    • 5 x 200 ul

CNY 1,280.00


Forward sequencing primer VP1.5, Reverse sequencing primer XL39, 100pmoles each
    • 100 pmol

CNY 480.00


PDLIM2 mouse monoclonal antibody, clone OTI10C4 (formerly 10C4)
    • 100 ul

CNY 1,999.00
CNY 2,700.00

Specifications

Product Data
Type Human Tagged ORF Clone
Tag TurboGFP
Synonyms MYSTIQUE; SLIM
Vector pCMV6-AC-GFP
E. coli Selection Ampicillin (100 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
>RG219644 representing NM_176871
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGGCGTTGACGGTGGATGTGGCCGGGCCAGCGCCCTGGGGCTTCCGTATCACAGGGGGCAGGGATTTCC
ACACGCCCATCATGGTGACTAAGGTGGCCGAGCGGGGCAAAGCCAAGGACGCTGACCTCCGGCCTGGAGA
CATAATCGTGGCCATCAACGGGGAAAGCGCGGAGGGCATGCTGCATGCCGAGGCCCAGAGCAAGATCCGC
CAGAGCCCCTCGCCCCTGCGGCTGCAGCTGGACCGGTCTCAGGCTACGTCTCCAGGGCAGACCAATGGGG
ACAGCTCCTTGGAAGTGCTGGCGACTCGCTTCCAGGGCTCCGTGAGGACATACACTGAGAGTCAGTCCTC
CTTAAGGTCCTCCTACTCCAGCCCAACCTCCCTCAGCCCGAGGGCCGGCAGCCCCTTCTCACCACCACCC
TCTAGCAGCTCCCTCACTGGAGAGGCAGCCATCAGCCGCAGCTTCCAGAGTCTGGCATGTTCCCCGGGCC
TCCCCGCTGCTGACCGCCTGTCCTACTCAGGCCGCCCTGGAAGCCGACAGGCCGGCCTCGGCCGCGCTGG
CGACTCGGCGGTGCTGGTGCTGCCGCCTTCCCCGGGCCCTCGTTCCTCCAGGCCCAGCATGGACTCGGAA
GGGGGAAGCCTCCTCCTGGACGAGGACTCGGAAGTCTTCAAGATGCTGCAGGAAAATCGCGAGGGACGGG
CGGCCCCCCGACAGTCCAGCTCCTTTCGGCTCTTGCAGGAAGCCCTGGAGGCTGAGGAGAGAGGTGGCAC
GCCAGCCTTCTTGCCCAGCTCACTGAGCCCCCAGTCCTCCCTGCCCGCCTCCAGGGCCCTGGCCACCCCT
CCCAAGCTCCACACTTGTGAGAAGTGCAGTACCAGCATCGCGAACCAGGCTGTGCGCATCCAGGAGGGCC
GGTACCGCCACCCCGGCTGCTACACCTGTGCCGACTGTGGGCTGAACCTGAAGATGCGCGGGCACTTCTG
GGAGGACGCTTGTGCTATGGAGGGAATGAGATTGTCACTGGAAGCTTTGGAGGGGATGGTGGAGGGCGCC
AAGCGGAGGGACAGGAGGAAGACCAGGAGACCCATCCAGCCAAGCTGG


ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
>RG219644 representing NM_176871
Red=Cloning site Green=Tags(s)

MALTVDVAGPAPWGFRITGGRDFHTPIMVTKVAERGKAKDADLRPGDIIVAINGESAEGMLHAEAQSKIR
QSPSPLRLQLDRSQATSPGQTNGDSSLEVLATRFQGSVRTYTESQSSLRSSYSSPTSLSPRAGSPFSPPP
SSSSLTGEAAISRSFQSLACSPGLPAADRLSYSGRPGSRQAGLGRAGDSAVLVLPPSPGPRSSRPSMDSE
GGSLLLDEDSEVFKMLQENREGRAAPRQSSSFRLLQEALEAEERGGTPAFLPSSLSPQSSLPASRALATP
PKLHTCEKCSTSIANQAVRIQEGRYRHPGCYTCADCGLNLKMRGHFWEDACAMEGMRLSLEALEGMVEGA
KRRDRRKTRRPIQPSW

TRTRPLE - GFP Tag - V
Restriction Sites SgfI-MluI      Cloning Scheme for this gene      Plasmid Map     
ACCN NM_176871
ORF Size 1098 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq NM_176871.5
RefSeq Size 4611 bp
RefSeq ORF 1101 bp
Locus ID 64236
UniProt ID Q96JY6
Gene Summary This gene encodes a member of the ALP subfamily of PDZ-LIM domain proteins. The encoded protein suppresses anchorage-dependent growth and promotes cell migration and adhesion through interactions with the actin cytoskeleton via the PDZ domain. The encoded protein is also a putative tumor suppressor protein, and decreased expression of this gene is associated with several malignancies including breast cancer and adult T-cell leukemia. Alternatively spliced transcript variants encoding multiple isoforms have been observed for this gene. [provided by RefSeq, Oct 2011]
*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

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