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DCAMKL2 (DCLK2) (NM_001040261) Human Tagged ORF Clone
CAT＃: RG219994
- TrueORF®
DCLK2 (tGFP-tagged) - Human doublecortin-like kinase 2 (DCLK2), transcript variant 2
ORF Plasmid: DDK
"NM_001040261" in other vectors (4)
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CNY 10,776.00
Product images
Specifications
| Product Data | |
| Type | Human Tagged ORF Clone |
| Tag | TurboGFP |
| Synonyms | CL2; CLICK-II; CLICK2; CLIK2; DCAMKL2; DCDC3; DCDC3B; DCK2 |
| Vector | pCMV6-AC-GFP |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>RG219994 representing NM_001040261
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGCCAGCACCAGGAGTATCGAGCTGGAGCACTTTGAGGAACGGGACAAAAGGCCGCGGCCGGGGTCGC GGAGAGGGGCCCCCAGCTCCTCCGGGGGCAGCAGCAGCTCGGGCCCCAAGGGGAACGGGCTCATCCCCAG TCCGGCGCACAGTGCCCACTGCAGCTTCTACCGCACGCGGACCCTGCAGGCCCTCAGCTCGGAGAAGAAG GCCAAGAAGGCGCGCTTCTACCGGAACGGGGACCGCTACTTCAAGGGCCTGGTGTTTGCCATCTCCAGCG ACCGCTTCCGGTCCTTCGATGCGCTCCTCATAGAGCTCACCCGCTCCCTGTCGGACAACGTGAACCTGCC CCAGGGTGTCCGCACTATCTACACCATCGACGGCAGCCGGAAGGTCACCAGCCTGGACGAGCTGCTGGAA GGTGAGAGTTACGTGTGTGCATCCAATGAACCATTTCGTAAAGTCGATTACACCAAAAATATTAATCCAA ACTGGTCTGTGAACATCAAGGGTGGGACATCCCGAGCGCTGGCTGCTGCCTCCTCTGTGAAAAGTGAAGT AAAAGAAAGTAAAGATTTCATCAAACCCAAGTTAGTGACTGTGATTCGAAGTGGAGTGAAGCCTAGAAAA GCCGTGCGGATCCTTCTGAATAAAAAGACTGCTCATTCCTTTGAACAAGTCTTAACAGATATCACCGAAG CCATTAAACTAGACTCAGGAGTCGTCAAGAGGCTCTGCACCCTGGATGGAAAGCAGGTTACTTGTCTGCA AGACTTTTTTGGTGATGACGATGTTTTTATTGCATGTGGACCAGAAAAATTTCGTTATGCCCAAGATGAC TTTGTCCTGGATCATAGTGAATGTCGTGTCCTGAAGTCATCTTATTCTCGATCCTCAGCTGTTAAGTATT CTGGATCCAAAAGCCCTGGGCCCTCTCGACGCAGCAAATCACCAGCTTCAGTAAAGCGGGGTGGCCACTA CTCCAGTGCCTATTCTACAGCCAAATCCCCAGTTAATGGAACTCCCAGCAGCCAACTTTCTACTCCTAAA TCTACGAAATCCTCCAGTTCCTCTCCAACTAGTCCAGGAAGTTTCAGAGGATTAAAGCAGATTTCTGCTC ATGGCAGATCTTCTTCCAATGTAAACGGTGGACCTGAGCTTGACCGTTGCATAAGTCCTGAAGGTGTGAA TGGAAACAGATGCTCTGAATCATCAACTCTTCTTGAGAAATACAAAATTGGAAAGGTCATTGGTGATGGC AATTTTGCAGTAGTCAAAGAGTGTATAGACAGGTCCACTGGAAAGGAGTTTGCCCTAAAGATTATAGACA AAGCCAAATGTTGTGGAAAGGAACACCTGATTGAGAATGAAGTGTCAATACTGCGCCGAGTGAAACATCC CAATATCATTATGCTGGTCGAGGAGATGGAAACAGCAACTGAGCTCTTTCTGGTGATGGAATTGGTCAAA GGTGGAGATCTCTTTGATGCAATTACTTCGTCGACCAAGTACACTGAGAGAGATGGCAGTGCCATGGTGT ACAACTTAGCCAATGCCCTCAGGTATCTCCATGGCCTCAGCATCGTGCACAGAGACATCAAACCAGAGAA TCTCTTGGTGTGTGAATATCCTGATGGAACCAAGTCTTTGAAACTGGGAGACTTTGGGCTTGCGACTGTG GTAGAAGGCCCTTTATACACAGTCTGTGGCACACCCACTTATGTGGCTCCAGAAATCATTGCTGAAACTG GCTATGGCCTGAAGGTGGACATTTGGGCAGCTGGTGTGATCACATACATACTTCTCTGTGGATTCCCACC ATTCCGAAGTGAGAACAATCTCCAGGAAGATCTCTTCGACCAGATCTTGGCTGGGAAGCTGGAGTTTCCG GCCCCCTACTGGGATAACATCACGGACTCTGCCAAGGAATTAATCAGTCAAATGCTTCAGGTAAATGTTG AAGCTCGGTGTACCGCGGGACAAATCCTGAGTCACCCCTGGGTGTCAGATGATGCCTCCCAGGAGAATAA CATGCAAGCTGAGGTGACAGGTAAACTAAAACAGCACTTTAATAATGCGCTCCCCAAACAGAACAGCACT ACCACCGGGGTCTCCGTCATCATGAACACGGCTCTAGATAAGGAGGGGCAGATTTTCTGCAGCAAGCACT GTCAAGACAGCGGCAGGCCTGGGATGGAGCCCATCTCTCCAGTTCCTCCCTCAGTGGAGGAGATCCCTGT GCCTGGGGAAGCAGTCCCGGCCCCCACCCCTCCGGAATCTCCCACCCCCCACCCTCCTCCCGCTGCCCCG GGTGGTGAGCGGGCAGGAACCTGGCGCCGCCACCGAGAC ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA >RG219994 representing NM_001040261
Red=Cloning site Green=Tags(s) MASTRSIELEHFEERDKRPRPGSRRGAPSSSGGSSSSGPKGNGLIPSPAHSAHCSFYRTRTLQALSSEKK AKKARFYRNGDRYFKGLVFAISSDRFRSFDALLIELTRSLSDNVNLPQGVRTIYTIDGSRKVTSLDELLE GESYVCASNEPFRKVDYTKNINPNWSVNIKGGTSRALAAASSVKSEVKESKDFIKPKLVTVIRSGVKPRK AVRILLNKKTAHSFEQVLTDITEAIKLDSGVVKRLCTLDGKQVTCLQDFFGDDDVFIACGPEKFRYAQDD FVLDHSECRVLKSSYSRSSAVKYSGSKSPGPSRRSKSPASVKRGGHYSSAYSTAKSPVNGTPSSQLSTPK STKSSSSSPTSPGSFRGLKQISAHGRSSSNVNGGPELDRCISPEGVNGNRCSESSTLLEKYKIGKVIGDG NFAVVKECIDRSTGKEFALKIIDKAKCCGKEHLIENEVSILRRVKHPNIIMLVEEMETATELFLVMELVK GGDLFDAITSSTKYTERDGSAMVYNLANALRYLHGLSIVHRDIKPENLLVCEYPDGTKSLKLGDFGLATV VEGPLYTVCGTPTYVAPEIIAETGYGLKVDIWAAGVITYILLCGFPPFRSENNLQEDLFDQILAGKLEFP APYWDNITDSAKELISQMLQVNVEARCTAGQILSHPWVSDDASQENNMQAEVTGKLKQHFNNALPKQNST TTGVSVIMNTALDKEGQIFCSKHCQDSGRPGMEPISPVPPSVEEIPVPGEAVPAPTPPESPTPHPPPAAP GGERAGTWRRHRD TRTRPLE - GFP Tag - V |
| Chromatograms |
CHROMATOGRAMS
 Sequencher program is needed, download here. |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_001040261 |
| ORF Size | 2349 bp |
| OTI Disclaimer | Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery. The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_001040261.5 |
| RefSeq Size | 4316 bp |
| RefSeq ORF | 2352 bp |
| Locus ID | 166614 |
| UniProt ID | Q8N568 |
| Protein Families | Druggable Genome, Protein Kinase |
| Gene Summary | This gene encodes a member of the protein kinase superfamily and the doublecortin family. The protein encoded by this gene contains two N-terminal doublecortin domains, which bind microtubules and regulate microtubule polymerization, a C-terminal serine/threonine protein kinase domain, which shows substantial homology to Ca2+/calmodulin-dependent protein kinase, and a serine/proline-rich domain in between the doublecortin and the protein kinase domains, which mediates multiple protein-protein interactions. The microtubule-polymerizing activity of the encoded protein is independent of its protein kinase activity. Mouse studies show that the DCX gene, another family member, and this gene share function in the establishment of hippocampal organization and that their absence results in a severe epileptic phenotype and lethality, as described in human patients with lissencephaly. Multiple alternatively spliced transcript variants have been identified. [provided by RefSeq, Sep 2010] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC219994 | DCLK2 (Myc-DDK-tagged)-Human doublecortin-like kinase 2 (DCLK2), transcript variant 2 |
CNY 8,080.00 |
|
| RC219994L3 | Lenti-ORF clone of DCLK2 (Myc-DDK-tagged)-Human doublecortin-like kinase 2 (DCLK2), transcript variant 2 |
CNY 9,980.00 |
|
| RC219994L4 | Lenti-ORF clone of DCLK2 (mGFP-tagged)-Human doublecortin-like kinase 2 (DCLK2), transcript variant 2 |
CNY 9,980.00 |
|
| SC310975 | DCLK2 (untagged)-Human doublecortin-like kinase 2 (DCLK2), transcript variant 2 |
CNY 14,820.00 |
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