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BFSP2 (NM_003571) Human Tagged ORF Clone
CAT＃: RG219996
- TrueORF®
BFSP2 (tGFP-tagged) - Human beaded filament structural protein 2, phakinin (BFSP2)
ORF Plasmid: DDK
"NM_003571" in other vectors (4)
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CNY 5,256.00
Product images
CNY 1,999.00
CNY 2,700.00
Specifications
| Product Data | |
| Type | Human Tagged ORF Clone |
| Tag | TurboGFP |
| Synonyms | CP47; CP49; CTRCT12; LIFL-L; PHAKOSIN |
| Vector | pCMV6-AC-GFP |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>RG219996 representing NM_003571
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGAGTGAGAGGCGAGTGGTAGTGGACTTGCCCACCAGTGCCAGCTCCAGCATGCCCCTCCAGAGGCGCA GGGCGTCCTTCAGGGGGCCACGGTCATCATCCTCCCTGGAGAGCCCCCCAGCCTCCAGGACCAATGCCAT GAGTGGCCTTGTCCGAGCACCCGGGGTCTATGTAGGAACAGCACCCAGTGGGTGCATAGGTGGCTTGGGT GCCCGTGTGACCCGCCGGGCCCTCGGCATCAGCAGTGTCTTCCTTCAGGGCCTGCGGAGCTCAGGCCTGG CCACCGTGCCGGCTCCAGGTTTGGAGAGGGACCATGGTGCTGTTGAGGACCTAGGGGGCTGCCTGGTGGA ATATATGGCCAAAGTGCACGCCCTTGAGCAAGTCAGTCAGGAGCTGGAAACACAACTGCGGATGCACCTG GAGAGCAAAGCCACACGCTCGGGAAACTGGGGTGCCCTACGGGCTTCCTGGGCCAGCAGCTGCCAGCAGG TGGGTGAGGCAGTCTTGGAAAATGCCCGGCTCATGCTGCAGACAGAAACTATCCAGGCCGGAGCAGATGA CTTTAAAGAGAGATATGAAAATGAGCAGCCATTTCGAAAGGCAGCAGAAGAGGAAATTAACTCTCTGTAT AAAGTCATTGATGAGGCTAATTTGACTAAAATGGACCTGGAGAGTCAAATAGAAAGTCTGAAAGAAGAAC TTGGCTCTCTATCAAGAAACTATGAAGAGGATGTGAAGCTGCTGCACAAACAGTTGGCAGGGTGTGAGCT GGAACAAATGGATGCTCCCATTGGCACTGGTCTGGACGACATCCTTGAGACGATCAGAATTCAGTGGGAG AGAGATGTTGAAAAGAACCGGGTGGAGGCAGGAGCCCTGCTCCAAGCTAAGCAACAGGCGGAGGTGGCCC ACATGTCCCAGACCCAGGAGGAGAAGCTGGCAGCTGCCCTCAGGGTGGAGTTACACAACACTTCGTGCCA AGTCCAGAGCCTCCAGGCTGAGACAGAATCCTTACGTGCCCTGAAACGAGGCCTGGAGAACACCTTGCAC GATGCCAAGCACTGGCATGACATGGAGCTCCAGAACCTGGGCGCTGTGGTCGGCCGGCTGGAGGCGGAGC TCAGGGAAATCCGAGCGGAGGCGGAGCAGCAGCAACAGGAGCGCGCGCATCTGCTGGCCCGCAAGTGCCA GCTGCAGAAGGACGTGGCGTCCTACCACGCCCTGCTGGACAGGGAGGAGAGCGGC ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA >RG219996 representing NM_003571
Red=Cloning site Green=Tags(s) MSERRVVVDLPTSASSSMPLQRRRASFRGPRSSSSLESPPASRTNAMSGLVRAPGVYVGTAPSGCIGGLG ARVTRRALGISSVFLQGLRSSGLATVPAPGLERDHGAVEDLGGCLVEYMAKVHALEQVSQELETQLRMHL ESKATRSGNWGALRASWASSCQQVGEAVLENARLMLQTETIQAGADDFKERYENEQPFRKAAEEEINSLY KVIDEANLTKMDLESQIESLKEELGSLSRNYEEDVKLLHKQLAGCELEQMDAPIGTGLDDILETIRIQWE RDVEKNRVEAGALLQAKQQAEVAHMSQTQEEKLAAALRVELHNTSCQVQSLQAETESLRALKRGLENTLH DAKHWHDMELQNLGAVVGRLEAELREIRAEAEQQQQERAHLLARKCQLQKDVASYHALLDREESG TRTRPLE - GFP Tag - V |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_003571 |
| ORF Size | 1245 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_003571.3, NP_003562.1 |
| RefSeq Size | 1644 bp |
| RefSeq ORF | 1248 bp |
| Locus ID | 8419 |
| UniProt ID | Q13515 |
| Gene Summary | More than 99% of the vertebrate ocular lens is comprised of terminally differentiated lens fiber cells. Two lens-specific intermediate filament-like proteins, the protein product of this gene (phakinin), and filensin, are expressed only after fiber cell differentiation has begun. Both proteins are found in a structurally unique cytoskeletal element that is referred to as the beaded filament (BF). Mutations in this gene have been associated with juvenile-onset, progressive cataracts and Dowling-Meara epidermolysis bullosa simplex. [provided by RefSeq, Jun 2009] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC219996 | BFSP2 (Myc-DDK-tagged)-Human beaded filament structural protein 2, phakinin (BFSP2) |
CNY 3,656.00 |
|
| RC219996L3 | Lenti ORF clone of Human beaded filament structural protein 2, phakinin (BFSP2), Myc-DDK-tagged |
CNY 5,890.00 |
|
| RC219996L4 | Lenti ORF clone of Human beaded filament structural protein 2, phakinin (BFSP2), mGFP tagged |
CNY 5,890.00 |
|
| SC303349 | BFSP2 (untagged)-Human beaded filament structural protein 2, phakinin (BFSP2) |
CNY 7,220.00 |
