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ch TOG (CKAP5) (NM_014756) Human Tagged ORF Clone
CAT＃: RG221071
- TrueORF®
CKAP5 (tGFP-tagged) - Human cytoskeleton associated protein 5 (CKAP5), transcript variant 2
ORF Plasmid: DDK
"NM_014756" in other vectors (2)
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CNY 20,900.00
货期*
8周
规格
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经常一起买 (5)
Specifications
| Product Data | |
| Type | Human Tagged ORF Clone |
| Tag | TurboGFP |
| Synonyms | ch-TOG; CHTOG; MSPS; TOG; TOGp |
| Vector | pCMV6-AC-GFP |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>RG221071 representing NM_014756
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGGAGATGACAGTGAGTGGTTGAAACTGCCAGTTGATCAGAAATGTGAACACAAGCTGTGGAAAGCAA GGTTAAGTGGGTATGAAGAGGCCCTGAAGATCTTCCAGAAAATAAAGGATGAAAAGAGCCCAGAGTGGTC CAAATTTTTAGGATTGATCAAAAAATTTGTCACTGATTCCAATGCAGTGGTTCAATTGAAAGGATTAGAA GCTGCACTTGTTTATGTTGAAAATGCCCATGTAGCAGGAAAAACCACAGGAGAAGTTGTGTCAGGTGTTG TAAGTAAGGTGTTCAATCAACCTAAAGCTAAAGCCAAGGAGCTGGGCATAGAGATCTGTCTTATGTACAT AGAGATTGAGAAAGGAGAGGCTGTTCAAGAAGAGCTCCTGAAAGGCTTGGACAATAAGAATCCCAAGATC ATAGTGGCCTGTATAGAGACACTGAGGAAAGCCTTAAGTGAATTTGGTTCCAAAATCATCTTGCTTAAGC CAATTATCAAAGTGTTGCCAAAACTCTTTGAGTCTCGAGAGAAGGCTGTTCGAGATGAAGCCAAACTAAT TGCTGTGGAGATTTACAGATGGATTCGGGATGCTCTGAGACCCCCATTACAAAATATAAACTCTGTTCAG TTGAAAGAACTAGAAGAAGAATGGGTCAAACTGCCAACAAGTGCTCCTAGACCTACTCGATTTCTTCGTT CCCAACAAGAACTAGAAGCTAAATTGGAACAACAACAGTCTGCTGGTGGAGATGCTGAAGGAGGTGGTGA TGATGGTGATGAGGTGCCACAAATAGATGCTTATGAGCTTTTAGAAGCTGTAGAAATCCTTTCCAAACTT CCCAAAGACTTTTATGACAAAATTGAGGCAAAAAAATGGCAAGAGAGAAAAGAGGCCCTGGAGTCTGTAG AAGTACTAATAAAAAACCCCAAACTGGAAGCTGGCGATTATGCAGATTTAGTAAAAGCATTAAAGAAGGT TGTTGGAAAGGACACCAATGTCATGTTGGTGGCTTTGGCAGCAAAATGTCTTACTGGCCTGGCTGTTGGG CTAAGGAAGAAATTTGGACAATATGCAGGACATGTTGTGCCAACCATCTTGGAGAAATTCAAAGAGAAGA AACCTCAAGTGGTACAAGCCCTGCAGGAGGCAATTGATGCAATCTTCCTTACTACCACACTACAGAACAT CAGTGAGGATGTTTTAGCAGTAATGGATAATAAAAATCCAACCATCAAGCAGCAGACATCTCTTTTTATT GCAAGAAGTTTCCGCCACTGCACTGCTTCTACCCTGCCAAAGAGCTTGCTAAAGCCCTTTTGTGCTGCAC TACTTAAGCACATCAATGATTCTGCTCCTGAAGTCAGAGATGCCGCATTTGAAGCATTGGGTACTGCTTT GAAGGTGGTTGGCGAGAAAGCAGTAAACCCATTCCTAGCTGATGTGGACAAACTCAAGCTTGATAAGATC AAAGAATGTTCAGAAAAGGTAGAACTGATACATGGTAAGAAAGCTGGACTAGCTGCTGATAAGAAGGAAT TCAAACCTCTGCCTGGAAGGACTGCTGCTTCAGGGGCTGCAGGAGATAAGGACACAAAGGACATTTCTGC ACCCAAACCAGGACCTCTAAAAAAGGCACCTGCTGCTAAGGCTGGTGGGCCACCAAAAAAGGGGAAACCA GCTGCACCAGGAGGCGCAGGGAATACTGGAACCAAGAACAAGAAAGGACTGGAGACTAAAGAAATAGTGG AGCCTGAGCTCTCGATAGAAGTATGTGAAGAAAAAGCTTCAGCTGTTCTTCCCCCTACCTGTATACAGCT TCTTGACAGCAGTAACTGGAAAGAAAGGCTGGCTTGTATGGAAGAGTTCCAGAAGGCTGTTGAGCTAATG GACCGAACTGAAATGCCATGCCAGGCATTAGTGAGGATGCTAGCCAAGAAACCTGGATGGAAAGAAACTA ATTTTCAGGTGATGCAAATGAAGCTTCATATAGTTGCTTTGATTGCCCAGAAGGGAAATTTTTCCAAAAC GTCAGCTCAGGTTGTATTAGATGGCCTTGTGGACAAGATTGGAGATGTGAAATGTGGGAACAATGCAAAA GAAGCTATGACAGCAATAGCCGAAGCCTGTATGTTACCATGGACTGCTGAACAGGTTGTGTCAATGGCTT TCTCACAAAAGAATCCCAAAAATCAGTCAGAAACTCTGAATTGGCTATCAAATGCCATAAAAGAATTTGG TTTTTCTGGGTTGAATGTCAAAGCTTTCATTAGCAATGTGAAGACAGCTCTTGCTGCAACAAACCCAGCT GTGAGGACTGCTGCCATAACCCTGCTTGGCGTGATGTATCTGTATGTTGGTCCCTCTTTGCGAATGTTCT TTGAGGATGAGAAGCCTGCCCTCCTATCCCAGATAGATGCAGAATTTGAGAAGATGCAGGGACAAAGTCC ACCTGCTCCAACCAGAGGAATTTCCAAGCATAGCACAAGTGGTACAGATGAAGGAGAAGATGGAGATGAA CCAGATGACGGGAGCAATGATGTCGTTGATCTTTTGCCGAGGACGGAGATCAGTGATAAAATCACTTCAG AGTTGGTATCTAAGATTGGTGATAAGAATTGGAAGATTAGGAAAGAAGGCCTAGATGAAGTGGCAGGTAT TATTAATGACGCAAAATTTATCCAACCGAATATAGGTGAACTTCCAACTGCCTTGAAGGGTCGACTCAAT GATTCAAATAAAATCTTGGTACAGCAAACGCTGAATATCCTGCAACAACTGGCAGTAGCCATGGGCCCAA ATATTAAGCAACATGTAAAAAATTTAGGCATCCCTATCATCACAGTCCTTGGAGACAGCAAGAACAATGT TCGAGCTGCTGCCCTAGCGACTGTGAATGCTTGGGCAGAACAGACTGGCATGAAGGAATGGCTGGAAGGA GAAGATCTTTCTGAAGAGCTCAAAAAGGAAAATCCTTTCTTGAGGCAAGAGCTTCTGGGCTGGCTGGCTG AGAAACTACCTACTCTTCGTTCCACCCCTACAGACCTTATCCTTTGTGTTCCTCATCTCTACTCCTGCCT AGAAGATCGAAATGGAGATGTGCGAAAGAAGGCCCAAGATGCCTTGCCATTCTTCATGATGCATTTAGGA TATGAAAAAATGGCCAAGGCTACTGGGAAACTAAAGCCAACTTCTAAAGATCAGGTATTGGCCATGCTAG AGAAAGCCAAAGTTAACATGCCAGCCAAGCCTGCTCCACCCACTAAAGCAACTTCTAAACCAATGGGAGG GTCCGCTCCAGCCAAATTCCAGCCTGCATCAGCACCTGCTGAAGATTGTATTTCCAGCAGTACAGAACCC AAACCTGATCCAAAAAAGGCCAAAGCTCCAGGATTATCCTCTAAAGCAAAGAGTGCACAAGGGAAGAAGA TGCCAAGCAAAACCAGCTTAAAGGAGGATGAAGACAAATCCGGGCCTATTTTTATTGTTGTTCCAAATGG AAAAGAGCAAAGGATGAAAGATGAAAAAGGATTGAAGGTGCTAAAGTGGAATTTTACTACCCCACGGGAT GAATACATTGAGCAACTAAAGACTCAAATGTCTAGCTGTGTGGCTAAATGGTTACAAGATGAGATGTTTC ACTCAGACTTTCAGCATCATAACAAAGCCCTTGCTGTTATGGTTGATCACTTGGAGAGTGAAAAAGAAGG AGTTATTGGTTGCCTGGATCTTATCTTAAAGTGGCTTACCCTGAGGTTTTTTGACACCAATACAAGCGTC CTGATGAAAGCACTAGAATATTTAAAATTGCTCTTCACCTTGCTAAGTGAAGAAGAATATCATCTTACTG AGAATGAAGCATCTTCCTTCATCCCCTATCTTGTCGTCAAGGTTGGAGAACCAAAGGATGTCATTCGTAA AGATGTTCGTGCCATCCTGAACCGGATGTGCCTTGTCTACCCAGCTAGCAAGATGTTTCCCTTTATCATG GAAGGAACCAAATCCAAAAACTCTAAGCAGAGAGCAGAGTGCCTGGAAGAGCTGGGATGTCTGGTTGAGT CCTATGGCATGAATGTTTGCCAACCAACCCCAGGAAAAGCCTTAAAGGAAATAGCTGTTCACATAGGAGA CCGTGACAATGCTGTACGCAATGCTGCACTCAACACCATTGTAACGGTGTACAATGTACATGGGGATCAG GTGTTCAAACTGATTGGAAATCTTTCTGAAAAGGATATGAGCATGCTCGAGGAGAGGATTAAGCGGTCAG CAAAGAGACCCTCTGCTGCACCAATAAAACAGGTGGAAGAGAAACCTCAGCGTGCACAGAACATAAGCTC CAATGCCAACATGTTACGCAAGGGACCAGCTGAGGACATGTCTTCCAAACTCAACCAAGCCCGAAGCATG AGTGGGCATCCTGAGGCAGCCCAGATGGTCCGCCGAGAATTCCAGCTGGATCTAGATGAGATTGAGAATG ACAATGGTACAGTCCGATGTGAAATGCCAGAACTTGTTCAGCACAAACTGGATGACATTTTTGAGCCAGT CCTTATTCCTGAACCCAAGATCCGGGCTGTTTCTCCACACTTCGATGACATGCACAGTAATACAGCATCC ACAATCAATTTCATTATCTCCCAAGTAGCCAGTGGTGACATCAACACAAGTATCCAAGCTCTGACACAGC TGTTTCAGATAGAGAGCCTTGCCCGGGAGGCCTCCACTGGAGTACTAAAAGACCTAATGCATGGCCTCAT CACCTTAATGCTGGATTCTCGGATTGAAGATCTTGAGGAAGGACAACAGGTCATCCGCTCTGTGAACCTC TTGGTGGTGAAGGTTCTGGAGAAGTCAGACCAGACCAACATCCTGAGTGCCCTACTTGTTTTGCTCCAAG ACAGCCTGCTAGCAACAGCCAGTTCTCCCAAATTCTCAGAGCTTGTTATGAAGTGTCTCTGGAGAATGGT TCGACTGTTGCCTGATACCATCAATAGCATTAACCTAGACAGAATTCTTCTGGATATCCACATTTTCATG AAGGTCTTCCCCAAAGAGAAACTGAAGCAATGCAAAAGTGAATTTCCCATAAGGACCCTAAAGACCCTGC TACACACCTTATGCAAATTAAAAGGGCCCAAGATCCTGGACCACCTAACGATGATCGACAACAAAAACGA GTCTGAGCTGGAGGCCCATCTCTGCCGGATGATGAAGCACAGTATGGACCAGACTGGGAGCAAGTCTGAT AAGGAAACAGAAAAGGGAGCATCTCGAATAGATGAAAAATCATCAAAGGCCAAAGTGAATGATTTCTTAG CTGAGATTTTTAAGAAGATTGGCTCTAAAGAAAACACTAAAGAGGGACTAGCAGAGTTATATGAATATAA GAAGAAATACTCAGATGCTGACATTGAACCATTTCTGAAAAATTCCTCACAGTTCTTCCAGAGCTATGTC GAAAGAGGCCTTCGGGTGATTGAGATGGAGAGGGAGGGCAAAGGTCGTATTTCCACTTCAACAGGCATCT CCCCTCAGATGGAAGTCACATGTGTGCCCACGCCCACAAGCACAGTGTCCTCCATAGGTAACACAAATGG GGAAGAAGTGGGGCCATCTGTCTACTTGGAAAGGCTAAAGATCCTCCGACAGCGATGTGGTCTGGACAAC ACAAAGCAAGATGACCGACCTCCTTTGACCTCTTTGCTCTCCAAACCAGCAGTTCCTACTGTCGCCTCTT CCACAGACATGCTCCACAGCAAACTCTCTCAGCTCCGGGAGTCACGGGAGCAGCACCAGCATTCAGACCT GGATTCTAACCAGACTCACTCTTCAGGAACTGTGACCTCCTCCTCCTCCACAGCTAACATAGACGACTTG AAAAAAAGACTGGAGAGAATAAAGAGCAGTCGCAAA ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA >RG221071 representing NM_014756
Red=Cloning site Green=Tags(s) MGDDSEWLKLPVDQKCEHKLWKARLSGYEEALKIFQKIKDEKSPEWSKFLGLIKKFVTDSNAVVQLKGLE AALVYVENAHVAGKTTGEVVSGVVSKVFNQPKAKAKELGIEICLMYIEIEKGEAVQEELLKGLDNKNPKI IVACIETLRKALSEFGSKIILLKPIIKVLPKLFESREKAVRDEAKLIAVEIYRWIRDALRPPLQNINSVQ LKELEEEWVKLPTSAPRPTRFLRSQQELEAKLEQQQSAGGDAEGGGDDGDEVPQIDAYELLEAVEILSKL PKDFYDKIEAKKWQERKEALESVEVLIKNPKLEAGDYADLVKALKKVVGKDTNVMLVALAAKCLTGLAVG LRKKFGQYAGHVVPTILEKFKEKKPQVVQALQEAIDAIFLTTTLQNISEDVLAVMDNKNPTIKQQTSLFI ARSFRHCTASTLPKSLLKPFCAALLKHINDSAPEVRDAAFEALGTALKVVGEKAVNPFLADVDKLKLDKI KECSEKVELIHGKKAGLAADKKEFKPLPGRTAASGAAGDKDTKDISAPKPGPLKKAPAAKAGGPPKKGKP AAPGGAGNTGTKNKKGLETKEIVEPELSIEVCEEKASAVLPPTCIQLLDSSNWKERLACMEEFQKAVELM DRTEMPCQALVRMLAKKPGWKETNFQVMQMKLHIVALIAQKGNFSKTSAQVVLDGLVDKIGDVKCGNNAK EAMTAIAEACMLPWTAEQVVSMAFSQKNPKNQSETLNWLSNAIKEFGFSGLNVKAFISNVKTALAATNPA VRTAAITLLGVMYLYVGPSLRMFFEDEKPALLSQIDAEFEKMQGQSPPAPTRGISKHSTSGTDEGEDGDE PDDGSNDVVDLLPRTEISDKITSELVSKIGDKNWKIRKEGLDEVAGIINDAKFIQPNIGELPTALKGRLN DSNKILVQQTLNILQQLAVAMGPNIKQHVKNLGIPIITVLGDSKNNVRAAALATVNAWAEQTGMKEWLEG EDLSEELKKENPFLRQELLGWLAEKLPTLRSTPTDLILCVPHLYSCLEDRNGDVRKKAQDALPFFMMHLG YEKMAKATGKLKPTSKDQVLAMLEKAKVNMPAKPAPPTKATSKPMGGSAPAKFQPASAPAEDCISSSTEP KPDPKKAKAPGLSSKAKSAQGKKMPSKTSLKEDEDKSGPIFIVVPNGKEQRMKDEKGLKVLKWNFTTPRD EYIEQLKTQMSSCVAKWLQDEMFHSDFQHHNKALAVMVDHLESEKEGVIGCLDLILKWLTLRFFDTNTSV LMKALEYLKLLFTLLSEEEYHLTENEASSFIPYLVVKVGEPKDVIRKDVRAILNRMCLVYPASKMFPFIM EGTKSKNSKQRAECLEELGCLVESYGMNVCQPTPGKALKEIAVHIGDRDNAVRNAALNTIVTVYNVHGDQ VFKLIGNLSEKDMSMLEERIKRSAKRPSAAPIKQVEEKPQRAQNISSNANMLRKGPAEDMSSKLNQARSM SGHPEAAQMVRREFQLDLDEIENDNGTVRCEMPELVQHKLDDIFEPVLIPEPKIRAVSPHFDDMHSNTAS TINFIISQVASGDINTSIQALTQLFQIESLAREASTGVLKDLMHGLITLMLDSRIEDLEEGQQVIRSVNL LVVKVLEKSDQTNILSALLVLLQDSLLATASSPKFSELVMKCLWRMVRLLPDTINSINLDRILLDIHIFM KVFPKEKLKQCKSEFPIRTLKTLLHTLCKLKGPKILDHLTMIDNKNESELEAHLCRMMKHSMDQTGSKSD KETEKGASRIDEKSSKAKVNDFLAEIFKKIGSKENTKEGLAELYEYKKKYSDADIEPFLKNSSQFFQSYV ERGLRVIEMEREGKGRISTSTGISPQMEVTCVPTPTSTVSSIGNTNGEEVGPSVYLERLKILRQRCGLDN TKQDDRPPLTSLLSKPAVPTVASSTDMLHSKLSQLRESREQHQHSDLDSNQTHSSGTVTSSSSTANIDDL KKRLERIKSSRK TRTRPLE - GFP Tag - V |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_014756 |
| ORF Size | 5916 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_014756.3, NP_055571.2 |
| RefSeq Size | 6550 bp |
| RefSeq ORF | 5919 bp |
| Locus ID | 9793 |
| UniProt ID | Q14008 |
| Protein Families | Druggable Genome |
| Gene Summary | This gene encodes a cytoskeleton-associated protein which belongs to the TOG/XMAP215 family. The N-terminal half of this protein contains a microtubule-binding domain and the C-terminal half contains a KXGS motif for binding tubulin dimers. This protein has two distinct roles in spindle formation; it protects kinetochore microtubules from depolymerization and plays an essential role in centrosomal microtubule assembly. This protein may be necessary for the proper interaction of microtubules with the cell cortex for directional cell movement. It also plays a role in translation of the myelin basic protein (MBP) mRNA by interacting with heterogeneous nuclear ribonucleoprotein (hnRNP) A2, which associates with MBP. Alternatively spliced transcript variants encoding different isoforms have been identified. [provided by RefSeq, Aug 2011] |
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