ch TOG (CKAP5) (NM_014756) Human Untagged Clone

Specifications

Product Data
Type	Human Untagged Clone
Tag	Tag Free
Synonyms	ch-TOG; CHTOG; MSPS; TOG; TOGp
Vector	pCMV6-XL4
E. coli Selection	Ampicillin (100 ug/mL)
Mammalian Cell Selection	None
Sequence Data	Fully Sequenced ORF (showhide) >NCBI ORF sequence for NM_014756, the custom clone sequence may differ by one or more nucleotides ATGGGAGATGACAGTGAGTGGTTGAAACTGCCAGTTGATCAGAAATGTGAACACAAGCTGTGGAAAGCAA GGTTAAGTGGGTATGAAGAGGCCCTGAAGATCTTCCAGAAAATAAAGGATGAAAAGAGCCCAGAGTGGTC CAAATTTTTAGGATTGATCAAAAAATTTGTCACTGATTCCAATGCAGTGGTTCAATTGAAAGGATTAGAA GCTGCACTTGTTTATGTTGAAAATGCCCATGTAGCAGGAAAAACCACAGGAGAAGTTGTGTCAGGTGTTG TAAGTAAGGTGTTCAATCAACCTAAAGCTAAAGCCAAGGAGCTGGGCATAGAGATCTGTCTTATGTACAT AGAGATTGAGAAAGGAGAGGCTGTTCAAGAAGAGCTCCTGAAAGGCTTGGACAATAAGAATCCCAAGATC ATAGTGGCCTGTATAGAGACACTGAGGAAAGCCTTAAGTGAATTTGGTTCCAAAATCATCTTGCTTAAGC CAATTATCAAAGTGTTGCCAAAACTCTTTGAGTCTCGAGAGAAGGCTGTTCGAGATGAAGCCAAACTAAT TGCTGTGGAGATTTACAGATGGATTCGGGATGCTCTGAGACCCCCATTACAAAATATAAACTCTGTTCAG TTGAAAGAACTAGAAGAAGAATGGGTCAAACTGCCAACAAGTGCTCCTAGACCTACTCGATTTCTTCGTT CCCAACAAGAACTAGAAGCTAAATTGGAACAACAACAGTCTGCTGGTGGAGATGCTGAAGGAGGTGGTGA TGATGGTGATGAGGTGCCACAAATAGATGCTTATGAGCTTTTAGAAGCTGTAGAAATCCTTTCCAAACTT CCCAAAGACTTTTATGACAAAATTGAGGCAAAAAAATGGCAAGAGAGAAAAGAGGCCCTGGAGTCTGTAG AAGTACTAATAAAAAACCCCAAACTGGAAGCTGGCGATTATGCAGATTTAGTAAAAGCATTAAAGAAGGT TGTTGGAAAGGACACCAATGTCATGTTGGTGGCTTTGGCAGCAAAATGTCTTACTGGCCTGGCTGTTGGG CTAAGGAAGAAATTTGGACAATATGCAGGACATGTTGTGCCAACCATCTTGGAGAAATTCAAAGAGAAGA AACCTCAAGTGGTACAAGCCCTGCAGGAGGCAATTGATGCAATCTTCCTTACTACCACACTACAGAACAT CAGTGAGGATGTTTTAGCAGTAATGGATAATAAAAATCCAACCATCAAGCAGCAGACATCTCTTTTTATT GCAAGAAGTTTCCGCCACTGCACTGCTTCTACCCTGCCAAAGAGCTTGCTAAAGCCCTTTTGTGCTGCAC TACTTAAGCACATCAATGATTCTGCTCCTGAAGTCAGAGATGCCGCATTTGAAGCATTGGGTACTGCTTT GAAGGTGGTTGGCGAGAAAGCAGTAAACCCATTCCTAGCTGATGTGGACAAACTCAAGCTTGATAAGATC AAAGAATGTTCAGAAAAGGTAGAACTGATACATGGTAAGAAAGCTGGACTAGCTGCTGATAAGAAGGAAT TCAAACCTCTGCCTGGAAGGACTGCTGCTTCAGGGGCTGCAGGAGATAAGGACACAAAGGACATTTCTGC ACCCAAACCAGGACCTCTAAAAAAGGCACCTGCTGCTAAGGCTGGTGGGCCACCAAAAAAGGGGAAACCA GCTGCACCAGGAGGCGCAGGGAATACTGGAACCAAGAACAAGAAAGGACTGGAGACTAAAGAAATAGTGG AGCCTGAGCTCTCGATAGAAGTATGTGAAGAAAAAGCTTCAGCTGTTCTTCCCCCTACCTGTATACAGCT TCTTGACAGCAGTAACTGGAAAGAAAGGCTGGCTTGTATGGAAGAGTTCCAGAAGGCTGTTGAGCTAATG GACCGAACTGAAATGCCATGCCAGGCATTAGTGAGGATGCTAGCCAAGAAACCTGGATGGAAAGAAACTA ATTTTCAGGTGATGCAAATGAAGCTTCATATAGTTGCTTTGATTGCCCAGAAGGGAAATTTTTCCAAAAC GTCAGCTCAGGTTGTATTAGATGGCCTTGTGGACAAGATTGGAGATGTGAAATGTGGGAACAATGCAAAA GAAGCTATGACAGCAATAGCCGAAGCCTGTATGTTACCATGGACTGCTGAACAGGTTGTGTCAATGGCTT TCTCACAAAAGAATCCCAAAAATCAGTCAGAAACTCTGAATTGGCTATCAAATGCCATAAAAGAATTTGG TTTTTCTGGGTTGAATGTCAAAGCTTTCATTAGCAATGTGAAGACAGCTCTTGCTGCAACAAACCCAGCT GTGAGGACTGCTGCCATAACCCTGCTTGGCGTGATGTATCTGTATGTTGGTCCCTCTTTGCGAATGTTCT TTGAGGATGAGAAGCCTGCCCTCCTATCCCAGATAGATGCAGAATTTGAGAAGATGCAGGGACAAAGTCC ACCTGCTCCAACCAGAGGAATTTCCAAGCATAGCACAAGTGGTACAGATGAAGGAGAAGATGGAGATGAA CCAGATGACGGGAGCAATGATGTCGTTGATCTTTTGCCGAGGACGGAGATCAGTGATAAAATCACTTCAG AGTTGGTATCTAAGATTGGTGATAAGAATTGGAAGATTAGGAAAGAAGGCCTAGATGAAGTGGCAGGTAT TATTAATGACGCAAAATTTATCCAACCGAATATAGGTGAACTTCCAACTGCCTTGAAGGGTCGACTCAAT GATTCAAATAAAATCTTGGTACAGCAAACGCTGAATATCCTGCAACAACTGGCAGTAGCCATGGGCCCAA ATATTAAGCAACATGTAAAAAATTTAGGCATCCCTATCATCACAGTCCTTGGAGACAGCAAGAACAATGT TCGAGCTGCTGCCCTAGCGACTGTGAATGCTTGGGCAGAACAGACTGGCATGAAGGAATGGCTGGAAGGA GAAGATCTTTCTGAAGAGCTCAAAAAGGAAAATCCTTTCTTGAGGCAAGAGCTTCTGGGCTGGCTGGCTG AGAAACTACCTACTCTTCGTTCCACCCCTACAGACCTTATCCTTTGTGTTCCTCATCTCTACTCCTGCCT AGAAGATCGAAATGGAGATGTGCGAAAGAAGGCCCAAGATGCCTTGCCATTCTTCATGATGCATTTAGGA TATGAAAAAATGGCCAAGGCTACTGGGAAACTAAAGCCAACTTCTAAAGATCAGGTATTGGCCATGCTAG AGAAAGCCAAAGTTAACATGCCAGCCAAGCCTGCTCCACCCACTAAAGCAACTTCTAAACCAATGGGAGG GTCCGCTCCAGCCAAATTCCAGCCTGCATCAGCACCTGCTGAAGATTGTATTTCCAGCAGTACAGAACCC AAACCTGATCCAAAAAAGGCCAAAGCTCCAGGATTATCCTCTAAAGCAAAGAGTGCACAAGGGAAGAAGA TGCCAAGCAAAACCAGCTTAAAGGAGGATGAAGACAAATCCGGGCCTATTTTTATTGTTGTTCCAAATGG AAAAGAGCAAAGGATGAAAGATGAAAAAGGATTGAAGGTGCTAAAGTGGAATTTTACTACCCCACGGGAT GAATACATTGAGCAACTAAAGACTCAAATGTCTAGCTGTGTGGCTAAATGGTTACAAGATGAGATGTTTC ACTCAGACTTTCAGCATCATAACAAAGCCCTTGCTGTTATGGTTGATCACTTGGAGAGTGAAAAAGAAGG AGTTATTGGTTGCCTGGATCTTATCTTAAAGTGGCTTACCCTGAGGTTTTTTGACACCAATACAAGCGTC CTGATGAAAGCACTAGAATATTTAAAATTGCTCTTCACCTTGCTAAGTGAAGAAGAATATCATCTTACTG AGAATGAAGCATCTTCCTTCATCCCCTATCTTGTCGTCAAGGTTGGAGAACCAAAGGATGTCATTCGTAA AGATGTTCGTGCCATCCTGAACCGGATGTGCCTTGTCTACCCAGCTAGCAAGATGTTTCCCTTTATCATG GAAGGAACCAAATCCAAAAACTCTAAGCAGAGAGCAGAGTGCCTGGAAGAGCTGGGATGTCTGGTTGAGT CCTATGGCATGAATGTTTGCCAACCAACCCCAGGAAAAGCCTTAAAGGAAATAGCTGTTCACATAGGAGA CCGTGACAATGCTGTACGCAATGCTGCACTCAACACCATTGTAACGGTGTACAATGTACATGGGGATCAG GTGTTCAAACTGATTGGAAATCTTTCTGAAAAGGATATGAGCATGCTCGAGGAGAGGATTAAGCGGTCAG CAAAGAGACCCTCTGCTGCACCAATAAAACAGGTGGAAGAGAAACCTCAGCGTGCACAGAACATAAGCTC CAATGCCAACATGTTACGCAAGGGACCAGCTGAGGACATGTCTTCCAAACTCAACCAAGCCCGAAGCATG AGTGGGCATCCTGAGGCAGCCCAGATGGTCCGCCGAGAATTCCAGCTGGATCTAGATGAGATTGAGAATG ACAATGGTACAGTCCGATGTGAAATGCCAGAACTTGTTCAGCACAAACTGGATGACATTTTTGAGCCAGT CCTTATTCCTGAACCCAAGATCCGGGCTGTTTCTCCACACTTCGATGACATGCACAGTAATACAGCATCC ACAATCAATTTCATTATCTCCCAAGTAGCCAGTGGTGACATCAACACAAGTATCCAAGCTCTGACACAGC TGTTTCAGATAGAGAGCCTTGCCCGGGAGGCCTCCACTGGAGTACTAAAAGACCTAATGCATGGCCTCAT CACCTTAATGCTGGATTCTCGGATTGAAGATCTTGAGGAAGGACAACAGGTCATCCGCTCTGTGAACCTC TTGGTGGTGAAGGTTCTGGAGAAGTCAGACCAGACCAACATCCTGAGTGCCCTACTTGTTTTGCTCCAAG ACAGCCTGCTAGCAACAGCCAGTTCTCCCAAATTCTCAGAGCTTGTTATGAAGTGTCTCTGGAGAATGGT TCGACTGTTGCCTGATACCATCAATAGCATTAACCTAGACAGAATTCTTCTGGATATCCACATTTTCATG AAGGTCTTCCCCAAAGAGAAACTGAAGCAATGCAAAAGTGAATTTCCCATAAGGACCCTAAAGACCCTGC TACACACCTTATGCAAATTAAAAGGGCCCAAGATCCTGGACCACCTAACGATGATCGACAACAAAAACGA GTCTGAGCTGGAGGCCCATCTCTGCCGGATGATGAAGCACAGTATGGACCAGACTGGGAGCAAGTCTGAT AAGGAAACAGAAAAGGGAGCATCTCGAATAGATGAAAAATCATCAAAGGCCAAAGTGAATGATTTCTTAG CTGAGATTTTTAAGAAGATTGGCTCTAAAGAAAACACTAAAGAGGGACTAGCAGAGTTATATGAATATAA GAAGAAATACTCAGATGCTGACATTGAACCATTTCTGAAAAATTCCTCACAGTTCTTCCAGAGCTATGTC GAAAGAGGCCTTCGGGTGATTGAGATGGAGAGGGAGGGCAAAGGTCGTATTTCCACTTCAACAGGCATCT CCCCTCAGATGGAAGTCACATGTGTGCCCACGCCCACAAGCACAGTGTCCTCCATAGGTAACACAAATGG GGAAGAAGTGGGGCCATCTGTCTACTTGGAAAGGCTAAAGATCCTCCGACAGCGATGTGGTCTGGACAAC ACAAAGCAAGATGACCGACCTCCTTTGACCTCTTTGCTCTCCAAACCAGCAGTTCCTACTGTCGCCTCTT CCACAGACATGCTCCACAGCAAACTCTCTCAGCTCCGGGAGTCACGGGAGCAGCACCAGCATTCAGACCT GGATTCTAACCAGACTCACTCTTCAGGAACTGTGACCTCCTCCTCCTCCACAGCTAACATAGACGACTTG AAAAAAAGACTGGAGAGAATAAAGAGCAGTCGCAAATGA 5' Read Nucleotide Sequence (showhide) >OriGene 5' read for NM_014756 unedited GACTCCTATAGGCGGCCGCGAATTCGGCACGAGGAAACTGCTTACAGCAGGCCGCTTGGT TCTGACCCAGCTGAGGAAATACTCTTAATTCTAAGGAAAACCTGGAAGCACAATGGGAGA TGACAGTGAGTGGTTGAAACTGCCAGTTGATCAGAAATGTGAACACAAGCTGTGGAAAGC AAGGTTAAGTGGGTATGAAGAGGCCCTGAAGATCTTCCAGAAAATAAAGGATGAAAAGAG CCCAGAGTGGTCCAAATTTTTAGGATTGATCAAAAAATTTGTCACTGATTCCAATGCAGT GGTTCAATTGAAAGGATTAGAAGCTGCACTTGTTTATGTTGAAAATGCCCATGTAGCAGG AAAAACCACAGGAGAAGTTGTGTCATGTGTTGTAAGTAAGGTGTTCAATCAACCTAAAGC TAAAGCCAAGGAGCTGGGCATAGAGATCTGTCTTATGTACATAGAGATTGAGAAAGGAGA GGCTGTTCAAGAAGAGCTCCTGAAAGGCTTGGACAATAAGAATCCCAAGATCATAGTGGC CTGTATAGAGACACTGAGGAAAGCCTTAAGTGAATTTGGTTCCAAAATCATCTTGCTTAA GCCAATTATCAAAGTGTTGCCAAAACTCTTTGAGTCTCGAGAGAAGGCTGTTCGAGATGA AGCCAAACTAATTGCTGTGGAGATTTACAGATGGATTCGGGATGCTCTGAGACCCCCATT ACAAAATATAAACTCTGTTCAGTTGAAAGAACTAGAAGAAGAATGGGTCAAACTGCCAAC AAGTGCTCCTAGACCTACTCGATTNCTTCGTTCCCCAACAGACTAGAAGCTAAATGGNAA CACACAGTCTGCTGGTGGAATGCTGAANGAGTGGTGATGATGTGATGAGTGCACAAATAG AGCTATGAGCTTTAGAGCTGTAAAATCTTTTCAACTTCCAANGATTTT 3' Read Nucleotide Sequence (showhide) >OriGene 3' read for NM_014756 unedited GAGCACTGGGGCATGGGTCACACGGCATGCCACCCGGGTTCTGATCAGAAAACAGCTATG ACCGCGGCCGCAATCTAGAGACGAGTGTTAATTTTATTTTTTTCTTGACTAACGCAAACT TTATTTATAAAACACTCTTAAATTAATTGTATCATGCCGTGCATTCATACAGAATACAGT GACCCTTCTGGGCCACAGATAAAATCTCACGCACTGAAAGGAAAACTATTAAAGAGTTTC TCATCTTAACTATTAAAAAGCTAGGAAAGCTTATGACAATTTTACAAATGAGCAATTAAA AAGAAAAGAAAAGGGTCTGGGAACTAGACTGATCAGAGAGGGGGCAGCTGTTTACAAGTC TGTACACTAAACTCATCCACGGACAGTCTTCTAAAGCAGCAGGACGCTGACAAAGAGAAG CAGAGTATGTACAAATTCTTGTGCCACACTGACTCCTCCCCCTAGCTGCGCGGCATGATA CATACAACCAGTTTGTATACACTAGGCCTGCTGAGGCCATTTTAAACTATGAGGACTTCT AGTTTATTAAACTAAAGCTGCNGGGTGCCGGGAGAGTGGGGCAACTTCATTTGCCACTGC TCTATATTCTCTCCGTCTTTTTTTCAATCCTCTATGTTAGCTGTGGAGGAGGAAGGAGCC ACACTTCCTGAAGATTGAGTCTGGGTAAAAACCAGGTCGGAACGCTGGCCCCGCTCCCCT GACTTCCCGAACAAAAAGATTTTGCTGGCAAACCTTTCCCTAGAAAAGCCCAACTTTTTT CCTTCTGGTTTTGAAACACAAACACAATATTGGGGGAATCGTCTTCTTCGGTCGTAGTGT CCAACGCACCCATTTTTTCNACGTCN
Restriction Sites	NotI-NotI
ACCN	NM_014756
Insert Size	7000 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	NM_014756.2, NP_055571.2
RefSeq Size	6550 bp
RefSeq ORF	5919 bp
Locus ID	9793
UniProt ID	Q14008
Protein Families	Druggable Genome
Gene Summary	This gene encodes a cytoskeleton-associated protein which belongs to the TOG/XMAP215 family. The N-terminal half of this protein contains a microtubule-binding domain and the C-terminal half contains a KXGS motif for binding tubulin dimers. This protein has two distinct roles in spindle formation; it protects kinetochore microtubules from depolymerization and plays an essential role in centrosomal microtubule assembly. This protein may be necessary for the proper interaction of microtubules with the cell cortex for directional cell movement. It also plays a role in translation of the myelin basic protein (MBP) mRNA by interacting with heterogeneous nuclear ribonucleoprotein (hnRNP) A2, which associates with MBP. Alternatively spliced transcript variants encoding different isoforms have been identified. [provided by RefSeq, Aug 2011] Transcript Variant: This variant (2) lacks an in-frame exon in the 3' coding region, compared to variant 1. The resulting isoform (b) lacks an internal segment, compared to isoform a.

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