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Tryptophan 5 hydroxylase 2 (TPH2) (NM_173353) Human Tagged ORF Clone
CAT＃: RG221193
- TrueORF®
TPH2 (tGFP-tagged) - Human tryptophan hydroxylase 2 (TPH2)
ORF Plasmid: DDK
"NM_173353" in other vectors (4)
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CNY 7,088.00
Product images
CNY 1,000.00
Specifications
| Product Data | |
| Type | Human Tagged ORF Clone |
| Tag | TurboGFP |
| Synonyms | ADHD7; NTPH |
| Vector | pCMV6-AC-GFP |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>RG221193 representing NM_173353
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGCAGCCAGCAATGATGATGTTTTCCAGTAAATACTGGGCACGGAGAGGGTTTTCCCTGGATTCAGCAG TGCCCGAAGAGCATCAGCTACTTGGCAGCTCAACACTAAATAAACCTAACTCTGGCAAAAATGACGACAA AGGCAACAAGGGAAGCAGCAAACGTGAAGCTGCTACCGAAAGTGGCAAGACAGCAGTTGTTTTCTCCTTG AAGAATGAAGTTGGTGGATTGGTAAAAGCACTGAGGCTCTTTCAGGAAAAACGTGTCAACATGGTTCATA TTGAATCCAGGAAATCTCGGCGAAGAAGTTCTGAGGTTGAAATCTTTGTGGACTGTGAGTGTGGGAAAAC AGAATTCAATGAGCTCATTCAGTTGCTGAAATTTCAAACCACTATTGTGACGCTGAATCCTCCAGAGAAC ATTTGGACAGAGGAAGAAGAGCTAGAGGATGTGCCCTGGTTCCCTCGGAAGATCTCTGAGTTAGACAAAT GCTCTCACAGAGTTCTCATGTATGGTTCTGAGCTTGATGCTGACCACCCAGGATTTAAGGACAATGTCTA TCGACAGAGAAGAAAGTATTTTGTGGATGTGGCCATGGGTTATAAATATGGTCAGCCCATTCCCAGGGTG GAGTATACTGAAGAAGAAACTAAAACTTGGGGTGTTGTATTCCGGGAGCTCTCCAAACTCTATCCCACTC ATGCTTGCCGAGAGTATTTGAAAAACTTCCCTCTGCTGACTAAATACTGTGGCTACAGAGAGGACAATGT GCCTCAACTCGAAGATGTCTCCATGTTTCTGAAAGAAAGGTCTGGCTTCACGGTGAGGCCGGTGGCTGGA TACCTGAGCCCACGAGACTTTCTGGCAGGACTGGCCTACAGAGTGTTCCACTGTACCCAGTACATCCGGC ATGGCTCAGATCCCCTCTACACCCCGGAACCAGACACATGCCATGAACTCTTGGGACATGTTCCACTACT TGCGGATCCTAAGTTTGCTCAGTTTTCACAAGAAATAGGTCTGGCGTCTCTGGGAGCATCAGATGAAGAT GTTCAGAAACTAGCCACGTGCTATTTCTTCACAATCGAGTTTGGCCTTTGCAAGCAAGAAGGGCAACTGC GGGCTTATGGAGCAGGACTCCTTTCCTCCATTGGAGAATTAAAGCACGCCCTTTCTGACAAGGCATGTGT GAAAGCCTTTGACCCAAAGACAACTTGCTTACAGGAATGCCTTATCACCACCTTCCAGGAAGCCTACTTT GTTTCAGAAAGTTTTGAAGAAGCCAAAGAAAAGATGAGGGACTTTGCAAAGTCAATTACCCGTCCCTTCT CAGTATACTTCAATCCCTACACACAGAGTATTGAAATTCTGAAAGACACCAGAAGTATTGAAAATGTGGT GCAGGACCTTCGCAGCGACTTGAATACAGTGTGTGATGCTTTAAACAAAATGAACCAATATCTGGGGATT ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA >RG221193 representing NM_173353
Red=Cloning site Green=Tags(s) MQPAMMMFSSKYWARRGFSLDSAVPEEHQLLGSSTLNKPNSGKNDDKGNKGSSKREAATESGKTAVVFSL KNEVGGLVKALRLFQEKRVNMVHIESRKSRRRSSEVEIFVDCECGKTEFNELIQLLKFQTTIVTLNPPEN IWTEEEELEDVPWFPRKISELDKCSHRVLMYGSELDADHPGFKDNVYRQRRKYFVDVAMGYKYGQPIPRV EYTEEETKTWGVVFRELSKLYPTHACREYLKNFPLLTKYCGYREDNVPQLEDVSMFLKERSGFTVRPVAG YLSPRDFLAGLAYRVFHCTQYIRHGSDPLYTPEPDTCHELLGHVPLLADPKFAQFSQEIGLASLGASDED VQKLATCYFFTIEFGLCKQEGQLRAYGAGLLSSIGELKHALSDKACVKAFDPKTTCLQECLITTFQEAYF VSESFEEAKEKMRDFAKSITRPFSVYFNPYTQSIEILKDTRSIENVVQDLRSDLNTVCDALNKMNQYLGI TRTRPLE - GFP Tag - V |
| Chromatograms |
CHROMATOGRAMS
 Sequencher program is needed, download here. |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_173353 |
| ORF Size | 1470 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq | NM_173353.3, NP_775489.2 |
| RefSeq Size | 2360 bp |
| RefSeq ORF | 1473 bp |
| Locus ID | 121278 |
| UniProt ID | Q8IWU9 |
| Protein Families | Druggable Genome |
| Protein Pathways | Metabolic pathways, Tryptophan metabolism |
| Gene Summary | This gene encodes a member of the pterin-dependent aromatic acid hydroxylase family. The encoded protein catalyzes the first and rate limiting step in the biosynthesis of serotonin, an important hormone and neurotransmitter. Mutations in this gene may be associated with psychiatric diseases such as bipolar affective disorder and major depression. [provided by RefSeq, Feb 2016] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC221193 | TPH2 (Myc-DDK-tagged)-Human tryptophan hydroxylase 2 (TPH2) |
CNY 5,488.00 |
|
| RC221193L3 | Lenti-ORF clone of TPH2 (Myc-DDK-tagged)-Human tryptophan hydroxylase 2 (TPH2) |
CNY 7,888.00 |
|
| RC221193L4 | Lenti-ORF clone of TPH2 (mGFP-tagged)-Human tryptophan hydroxylase 2 (TPH2) |
CNY 5,890.00 |
|
| SC313700 | TPH2 (untagged)-Human tryptophan hydroxylase 2 (TPH2) |
CNY 5,488.00 |
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