MIB1 (NM_020774) Human Tagged ORF Clone
CAT#: RG221377
- TrueORF®
MIB1 (tGFP-tagged) - Human mindbomb homolog 1 (Drosophila) (MIB1)
ORF Plasmid: DDK
"NM_020774" in other vectors (6)
Need custom modification / cloning service?
Get a free quote
CNY 8,608.00
Specifications
Product Data | |
Type | Human Tagged ORF Clone |
Tag | TurboGFP |
Synonyms | DIP-1; DIP1; LVNC7; MIB; ZZANK2; ZZZ6 |
Vector | pCMV6-AC-GFP |
E. coli Selection | Ampicillin (100 ug/mL) |
Mammalian Cell Selection | Neomycin |
Sequence Data |
>RG221377 representing NM_020774
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGAGTAACTCCCGGAATAACCGGGTGATGGTGGAAGGGGTTGGCGCTCGGGTAGTGCGCGGCCCGGACT GGAAGTGGGGGAAGCAGGACGGCGGCGAGGGCCATGTGGGCACCGTCCGGAGCTTCGAGAGCCCCGAGGA GGTGGTGGTAGTGTGGGACAACGGCACAGCTGCCAACTACCGCTGCTCCGGGGCTTACGACCTCCGCATC CTGGACAGCGCGCCCACCGGCATCAAGCATGATGGAACCATGTGTGATACCTGCCGCCAGCAACCAATCA TTGGCATTCGATGGAAGTGTGCAGAGTGTACAAATTATGATTTGTGCACAGTGTGTTATCATGGAGATAA ACATCATTTAAGACATCGCTTTTACCGAATTACTACACCGGGAAGTGAGAGGGTTCTGTTAGAGTCTCGT AGGAAATCTAAGAAGATTACAGCCAGAGGAATCTTTGCAGGTGCCAGAGTGGTGCGAGGAGTGGACTGGC AGTGGGAAGATCAAGATGGAGGAAATGGACGTAGGGGAAAGGTAACAGAAATCCAGGACTGGAGTGCATC AAGCCCACATAGCGCAGCATATGTCCTCTGGGATAATGGTGCTAAGAACCTTTACAGAGTTGGCTTTGAG GGCATGTCTGATCTGAAATGTGTCCAGGATGCCAAGGGAGGTTCTTTCTACAGAGATCACTGCCCTGTGC TAGGTGAGCAGAATGGCAACAGGAATCCTGGTGGATTGCAGATTGGTGACCTGGTAAATATAGATCTCGA CCTCGAAATTGTACAGTCTTTGCAGCATGGTCATGGAGGATGGACTGATGGAATGTTTGAGACTTTAACT ACAACTGGAACTGTTTGTGGCATTGATGAAGATCATGACATTGTAGTACAGTATCCAAGTGGCAATAGGT GGACCTTCAATCCTGCTGTTCTCACTAAAGCGAACATTGTCCGAAGTGGAGATGCTGCTCAGGGTGCAGA AGGAGGCACCTCGCAGTTTCAAGTGGGTGATCTTGTACAAGTTTGTTATGACCTGGAACGAATTAAACTT CTACAAAGAGGACATGGAGAATGGGCTGAAGCGATGCTTCCAACTTTAGGTAAAGTTGGCCGAGTACAAC AGATTTATTCAGACAGTGATTTAAAGGTGGAAGTTTGTGGAACATCTTGGACATACAATCCAGCAGCAGT TTCCAAGGTGGCATCTGCAGGATCAGCCATTAGCAATGCATCTGGTGAAAGACTCTCACAACTCCTGAAG AAATTATTTGAAACCCAAGAATCTGGTGACCTCAATGAAGAATTAGTTAAGGCTGCTGCCAATGGAGATG TTGCTAAAGTGGAAGATTTGCTTAAAAGACCAGATGTGGATGTAAATGGGCAATGTGCTGGCCACACAGC TATGCAAGCTGCTAGTCAGAATGGACATGTTGACATTTTGAAGTTACTTTTGAAGCAAAACGTGGATGTC GAAGCAGAGGATAAAGATGGTGATAGAGCAGTTCACCATGCAGCTTTTGGAGATGAAGGCGCTGTTATAG AAGTACTACATCGAGGTAGTGCTGATTTGAATGCTCGAAACAAGCGCCGACAGACACCACTTCATATTGC TGTCAATAAAGGTCATCTTCAAGTTGTGAAGACTTTATTGGACTTTGGCTGTCATCCCAGTCTCCAGGAT TCTGAAGGTGATACCCCTCTTCATGATGCAATAAGTAAGAAACGTGATGATATCCTAGCAGTTCTTTTGG AAGCTGGAGCAGATGTTACCATCACAAACAATAATGGATTTAATGCTCTGCATCATGCTGCACTAAGGGG AAATCCCAGTGCAATGCGTGTTTTACTATCTAAATTACCAAGACCATGGATTGTGGATGAGAAGAAAGAT GATGGTTATACTGCCTTACATCTGGCTGCCCTTAATAATCACGTAGAAGTGGCTGAACTGTTGGTACATC AGGGTAATGCAAACCTGGATATCCAGAATGTGAACCAACAAACTGCCCTACACCTTGCTGTTGAACGACA GCATACCCAGATTGTTAGGCTTTTGGTCCGTGCAGGTGCCAAGCTTGATATTCAGGATAAGGATGGGGAT ACTCCTTTGCATGAAGCTCTAAGGCATCACACTTTGTCTCAGCTACGTCAGCTCCAAGATATGCAAGATG TGGGGAAGGTGGATGCTGCCTGGGAGCCATCCAAAAACACGTTAATAATGGGACTTGGTACCCAGGGGGC AGAGAAGAAGAGTGCAGCATCTATTGCCTGTTTCTTGGCAGCCAATGGTGCTGACCTGAGCATTCGAAAT AAGAAGGGTCAATCGCCACTTGATCTCTGTCCTGATCCGAATCTCTGCAAAGCACTGGCAAAGTGTCATA AGGAAAAAGTCAGTGGTCAAGTGGGTTCTCGGAGTCCTTCTATGATTAGTAATGATTCTGAAACCTTAGA AGAGTGTATGGTGTGCTCAGATATGAAGAGAGATACTCTTTTTGGTCCATGTGGACATATTGCTACCTGT TCTTTATGTTCTCCACGTGTCAAGAAATGCCTCATCTGTAAAGAACAGGTTCAATCCAGGACAAAGATTG AAGAATGTGTGGTATGCTCTGACAAGAAAGCAGCTGTTCTTTTTCAACCCTGTGGCCACATGTGTGCTTG TGAGAACTGTGCTAACCTGATGAAAAAGTGTGTGCAGTGTCGAGCAGTAGTTGAACGAAGAGTGCCTTTC ATTATGTGCTGTGGAGGGAAAAGTTCAGAAGATGCCACTGATGATATCTCAAGTGGGAATATTCCAGTAT TACAAAAGGACAAGGATAATACCAATGTCAATGCAGATGTGCAAAAGTTGCAGCAACAGTTACAAGACAT TAAAGAGCAGACAATGTGCCCTGTGTGTCTAGATCGTCTGAAGAATATGATTTTCCTTTGTGGTCACGGA ACCTGTCAACTCTGTGGAGACCGCATGAGTGAATGTCCTATCTGTCGCAAGGCTATTGAACGAAGGATTC TTTTGTAT ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA >RG221377 representing NM_020774
Red=Cloning site Green=Tags(s) MSNSRNNRVMVEGVGARVVRGPDWKWGKQDGGEGHVGTVRSFESPEEVVVVWDNGTAANYRCSGAYDLRI LDSAPTGIKHDGTMCDTCRQQPIIGIRWKCAECTNYDLCTVCYHGDKHHLRHRFYRITTPGSERVLLESR RKSKKITARGIFAGARVVRGVDWQWEDQDGGNGRRGKVTEIQDWSASSPHSAAYVLWDNGAKNLYRVGFE GMSDLKCVQDAKGGSFYRDHCPVLGEQNGNRNPGGLQIGDLVNIDLDLEIVQSLQHGHGGWTDGMFETLT TTGTVCGIDEDHDIVVQYPSGNRWTFNPAVLTKANIVRSGDAAQGAEGGTSQFQVGDLVQVCYDLERIKL LQRGHGEWAEAMLPTLGKVGRVQQIYSDSDLKVEVCGTSWTYNPAAVSKVASAGSAISNASGERLSQLLK KLFETQESGDLNEELVKAAANGDVAKVEDLLKRPDVDVNGQCAGHTAMQAASQNGHVDILKLLLKQNVDV EAEDKDGDRAVHHAAFGDEGAVIEVLHRGSADLNARNKRRQTPLHIAVNKGHLQVVKTLLDFGCHPSLQD SEGDTPLHDAISKKRDDILAVLLEAGADVTITNNNGFNALHHAALRGNPSAMRVLLSKLPRPWIVDEKKD DGYTALHLAALNNHVEVAELLVHQGNANLDIQNVNQQTALHLAVERQHTQIVRLLVRAGAKLDIQDKDGD TPLHEALRHHTLSQLRQLQDMQDVGKVDAAWEPSKNTLIMGLGTQGAEKKSAASIACFLAANGADLSIRN KKGQSPLDLCPDPNLCKALAKCHKEKVSGQVGSRSPSMISNDSETLEECMVCSDMKRDTLFGPCGHIATC SLCSPRVKKCLICKEQVQSRTKIEECVVCSDKKAAVLFQPCGHMCACENCANLMKKCVQCRAVVERRVPF IMCCGGKSSEDATDDISSGNIPVLQKDKDNTNVNADVQKLQQQLQDIKEQTMCPVCLDRLKNMIFLCGHG TCQLCGDRMSECPICRKAIERRILLY TRTRPLE - GFP Tag - V |
Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
![]() |
ACCN | NM_020774 |
ORF Size | 3018 bp |
OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
Reference Data | |
RefSeq | NM_020774.4 |
RefSeq Size | 9308 bp |
RefSeq ORF | 3021 bp |
Locus ID | 57534 |
UniProt ID | Q86YT6 |
Protein Families | Druggable Genome |
Gene Summary | This gene encodes a protein containing multiple ankyrin repeats and RING finger domains that functions as an E3 ubiquitin ligase. The encoded protein positively regulates Notch signaling by ubiquitinating the Notch receptors, thereby facilitating their endocytosis. This protein may also promote the ubiquitination and degradation of death-associated protein kinase 1 (DAPK1). [provided by RefSeq, Jun 2013] |
Documents
Product Manuals |
FAQs |
SDS |
Resources
Other Versions
SKU | Description | Size | Price |
---|---|---|---|
RC221377 | MIB1 (Myc-DDK-tagged)-Human mindbomb homolog 1 (Drosophila) (MIB1) |
CNY 7,008.00 |
|
RC221377L1 | Lenti ORF clone of Human mindbomb homolog 1 (Drosophila) (MIB1), Myc-DDK-tagged |
CNY 9,408.00 |
|
RC221377L2 | Lenti ORF clone of Human mindbomb homolog 1 (Drosophila) (MIB1), mGFP tagged |
CNY 10,260.00 |
|
RC221377L3 | Lenti ORF clone of Human mindbomb homolog 1 (Drosophila) (MIB1), Myc-DDK-tagged |
CNY 10,260.00 |
|
RC221377L4 | Lenti ORF clone of Human mindbomb homolog 1 (Drosophila) (MIB1), mGFP tagged |
CNY 10,260.00 |
|
SC107162 | MIB1 (untagged)-Human mindbomb homolog 1 (Drosophila) (MIB1) |
CNY 7,016.00 |