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TMEM132A (NM_178031) Human Tagged ORF Clone
CAT＃: RG221527
- TrueORF®
TMEM132A (tGFP-tagged) - Human transmembrane protein 132A (TMEM132A), transcript variant 2
ORF Plasmid: DDK
"NM_178031" in other vectors (4)
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CNY 11,210.00
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Specifications
| Product Data | |
| Type | Human Tagged ORF Clone |
| Tag | TurboGFP |
| Synonyms | GBP; HSPA5BP1 |
| Vector | pCMV6-AC-GFP |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>RG221527 representing NM_178031
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGTGCGCGCGGATGGCCGGTCGCACAACAGCGGCCCCTCGGGGGCCCTACGGCCCCTGGCTCTGCCTCC TGGTGGCCCTCGCCCTGGACGTCGTGAGAGTGGACTGTGGCCAGGCTCCCCTGGACCCTGTCTACCTGCC GGCAGCCCTGGAGCTCCTAGACGCCCCTGAACACTTCCGTGTGCAGCAGGTGGGCCACTACCCACCTGCC AACTCCTCTCTGAGCTCCCGATCTGAGACCTTTCTGCTCCTACAGCCCTGGCCCAGGGCCCAGCCACTTC TCCGGGCCTCCTACCCACCTTTTGCCACTCAGCAGGTGGTCCCCCCTCGAGTCACTGAGCCCCACCAACG GCCAGTCCCATGGGACGTGCGGGCCGTTTCAGTGGAAGCGGCTGTGACTCCAGCAGAGCCCTACGCCCGG GTTCTCTTCCACCTCAAAGGGCAGGATTGGCCACCAGGGTCTGGCAGCCTGCCCTGTGCCCGGCTCCATG CCACACACCCTGCAGGCACTGCTCACCAAGCCTGCCGCTTCCAGCCATCCCTGGGCGCCTGCGTGGTGGA GCTGGAGCTTCCCTCGCACTGGTTCTCACAGGCCTCCACCACACGGGCCGAGCTGGCCTACACGCTTGAG CCTGCAGCTGAGGGCCCTGGGGGCTGTGGCTCCGGCGAGGAGAACGACCCTGGGGAGCAGGCCCTCCCAG TGGGGGGTGTGGAGCTGCGCCCAGCAGACCCCCCGCAGTACCAGGAGGTACCTCTGGACGAGGCTGTGAC TCTGCGGGTGCCTGACATGCCAGTGCGGCCCGGCCAGCTCTTTAGTGCTACCCTCCTGCTTCGGCACAAC TTCACAGCCAGCCTCCTGACCCTGCGGATCAAGGTGAAGAAGGGGCTGCATGTGACAGCCGCCCGCCCAG CCCAGCCCACACTCTGGACTGCCAAGCTAGACCGCTTCAAGGGCTCCAGGCACCACACCACCCTCATCAC CTGCCACCGTGCTGGGCTCACAGAGCCAGATTCCAGTCCCCTTGAACTGTCTGAGTTCCTATGGGTGGAC TTTGTGGTGGAGAATAGCACTGGTGGGGGCGTAGCGGTCACTCGCCCCGTCACGTGGCAGCTGGAGTACC CAGGCCAGGCCCCTGAAGCAGAGAAGGACAAAATGGTGTGGGAAATCCTGGTGTCTGAGCGGGACATCAG AGCCCTTATCCCACTGGCCAAGGCTGAGGAGCTGGTGAATACAGCACCACTGACTGGAGTGCCCCAGCAT GTCCCCGTGCGCCTTGTCACTGTGGACGGCGGGGGGGCCTTGGTGGAGGTGACAGAGCATGTCGGCTGCG AGTCTGCCAACACACAGGTCCTGCAGGTGTCTGAGGCCTGTGATGCCGTGTTCGTGGCTGGCAAGGAGAG CCGGGGCGCCCGGGGGGTGCGAGTGGACTTCTGGTGGCGCCGGCTCCGCGCCTCGCTGCGGCTGACCGTG TGGGCCCCCCTGCTACCGCTGCGTATCGAGCTCACCGACACCACCCTCGAGCAGGTCCGCGGCTGGAGGG TACCTGGCCCTGCTGAAGGGCCTGCGGAACCCGCTGCAGAGGCGTCGGATGAGGCCGAGCGGCGCGCCCG TGGCTGCCACCTGCAGTACCAGCGGGCCGGTGTGCGCTTCCTCGCCCCCTTCGCGGCCCACCCGCTGGAC GGCGGCCGCCGCCTCACGCACCTGCTTGGCCCCGACTGGCTGCTAGACGTGTCCCACCTCGTGGCGCCAC ACGCCCGCGTGCTGGACTCGCGTGTAGCCTCTCTGGAGGGTGGCCGTGTCGTGGTGGGCCGGGAGCCCGG TGTCACCTCCATTGAGGTGCGTTCCCCACTGTCTGACTCCATCCTGGGGGAGCAGGCGCTGGCTGTGACG GACGACAAGGTCTCAGTGCTGGAGCTGAGGGTGCAGCCAGTGATGGGCATCTCGCTGACCTTGAGCCGGG GCACTGCCCACCCCGGGGAGGTCACAGCTACGTGCTGGGCACAGTCAGCCCTTCCCGCCCCAAAGCAGGA GGTGGCCCTCTCCCTATGGCTGTCCTTCTCTGATCACACTGTGGCCCCAGCTGAGCTCTACGACCGCCGT GACCTGGGACTGTCCGTCTCAGCCGAGGAGCCTGGTGCCATCCTGCCAGCTGAGGAGCAGGGTGCCCAGC TCGGGGTGGTGGTGAGTGGGGCAGGCGCCGAGGGGCTGCCGCTGCATGTGGCTCTGCACCCGCCCGAGCC CTGCCGCCGGGGCCGCCACCGTGTGCCTCTGGCCTCTGGCACCGCCTGGCTGGGGCTGCCCCCTGCCTCC ACTCCAGCCCCTGCTCTCCCATCCAGCCCTGCTTGGAGCCCACCAGCCACAGAAGCCACCATGGGTGGTA AACGGCAGGTGGCAGGCAGTGTCGGGGGCAACACAGGTGTGAGGGGCAAGTTTGAGCGGGCAGAGGAGGA GGCCAGGAAGGAGGAGACCGAAGCCAGGGAGGAGGAGGAGGAAGAGGAGGAGGAGATGGTCCCTGCCCCT CAGCATGTCACTGAGCTAGAGCTGGGCATGTACGCCCTGCTGGGAGTCTTCTGCGTGGCCATCTTCATCT TCTTGGTCAATGGTGTGGTCTTCGTCCTGCGCTATCAGCGCAAAGAACCTCCCGACAGTGCCACTGACCC CACCTCCCCCCAGCCCCACAACTGGGTCTGGCTGGGCACTGACCAGGAGGAACTGAGCCGCCAGCTGGAC CGGCAGTCCCCTGGCCCGCCCAAGGGGGAGGGGAGCTGCCCCTGTGAGAGTGGGGGAGGAGGGGAGGCCC CTACCCTGGCCCCTGGCCCTCCTGGGGGCACCACCAGCTCCTCAAGCACCCTGGCCCGAAAGGAGGCTGG GGGGCGGCGGAAGCGAGTAGAGTTTGTGACATTTGCGCCAGCCCCTCCAGCCCAGTCACCTGAGGAGCCT GTAGGGGCCCCTGCTGTGCAGTCCATCCTTGTGGCAGGCGAGGAGGACATCCGCTGGGTGTGTGAGGACA TGGGGCTGAAGGACCCTGAGGAGCTTCGCAACTACATGGAGAGGATCCGGGGCAGCTCC ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA >RG221527 representing NM_178031
Red=Cloning site Green=Tags(s) MCARMAGRTTAAPRGPYGPWLCLLVALALDVVRVDCGQAPLDPVYLPAALELLDAPEHFRVQQVGHYPPA NSSLSSRSETFLLLQPWPRAQPLLRASYPPFATQQVVPPRVTEPHQRPVPWDVRAVSVEAAVTPAEPYAR VLFHLKGQDWPPGSGSLPCARLHATHPAGTAHQACRFQPSLGACVVELELPSHWFSQASTTRAELAYTLE PAAEGPGGCGSGEENDPGEQALPVGGVELRPADPPQYQEVPLDEAVTLRVPDMPVRPGQLFSATLLLRHN FTASLLTLRIKVKKGLHVTAARPAQPTLWTAKLDRFKGSRHHTTLITCHRAGLTEPDSSPLELSEFLWVD FVVENSTGGGVAVTRPVTWQLEYPGQAPEAEKDKMVWEILVSERDIRALIPLAKAEELVNTAPLTGVPQH VPVRLVTVDGGGALVEVTEHVGCESANTQVLQVSEACDAVFVAGKESRGARGVRVDFWWRRLRASLRLTV WAPLLPLRIELTDTTLEQVRGWRVPGPAEGPAEPAAEASDEAERRARGCHLQYQRAGVRFLAPFAAHPLD GGRRLTHLLGPDWLLDVSHLVAPHARVLDSRVASLEGGRVVVGREPGVTSIEVRSPLSDSILGEQALAVT DDKVSVLELRVQPVMGISLTLSRGTAHPGEVTATCWAQSALPAPKQEVALSLWLSFSDHTVAPAELYDRR DLGLSVSAEEPGAILPAEEQGAQLGVVVSGAGAEGLPLHVALHPPEPCRRGRHRVPLASGTAWLGLPPAS TPAPALPSSPAWSPPATEATMGGKRQVAGSVGGNTGVRGKFERAEEEARKEETEAREEEEEEEEEMVPAP QHVTELELGMYALLGVFCVAIFIFLVNGVVFVLRYQRKEPPDSATDPTSPQPHNWVWLGTDQEELSRQLD RQSPGPPKGEGSCPCESGGGGEAPTLAPGPPGGTTSSSSTLARKEAGGRRKRVEFVTFAPAPPAQSPEEP VGAPAVQSILVAGEEDIRWVCEDMGLKDPEELRNYMERIRGSS TRTRPLE - GFP Tag - V |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_178031 |
| ORF Size | 3069 bp |
| OTI Disclaimer | Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery. The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_178031.1, NP_821174.1 |
| RefSeq Size | 3483 bp |
| RefSeq ORF | 3072 bp |
| Locus ID | 54972 |
| UniProt ID | Q24JP5 |
| Protein Families | Transmembrane |
| Gene Summary | This gene encodes a protein that is highly similar to the rat Grp78-binding protein (GBP). Alternatively spliced transcript variants encoding different isoforms have been described. [provided by RefSeq, Jul 2008] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC221527 | TMEM132A (Myc-DDK-tagged)-Human transmembrane protein 132A (TMEM132A), transcript variant 2 |
CNY 10,688.00 |
|
| RC221527L3 | Lenti-ORF clone of TMEM132A (Myc-DDK-tagged)-Human transmembrane protein 132A (TMEM132A), transcript variant 2 |
CNY 12,070.00 |
|
| RC221527L4 | Lenti-ORF clone of TMEM132A (mGFP-tagged)-Human transmembrane protein 132A (TMEM132A), transcript variant 2 |
CNY 12,070.00 |
|
| SC315080 | TMEM132A (untagged)-Human transmembrane protein 132A (TMEM132A), transcript variant 2 |
CNY 7,840.00 |
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