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MUC4 (NM_138297) Human Tagged ORF Clone
CAT＃: RG222229
- TrueORF®
MUC4 (tGFP-tagged) - Human mucin 4, cell surface associated (MUC4), transcript variant 5
ORF Plasmid: DDK
"NM_138297" in other vectors (4)
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CNY 12,350.00
Product images
Specifications
| Product Data | |
| Type | Human Tagged ORF Clone |
| Tag | TurboGFP |
| Synonyms | ASGP; HSA276359; MUC-4 |
| Vector | pCMV6-AC-GFP |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>RG222229 representing NM_138297
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGAAGGGGGCACGCTGGAGGAGGGTCCCCTGGGTGTCCCTGAGCTGCCTGTGTCTCTGCCTCCTTCCGC ATGTGGTCCCAGGAGTTTCCCTCTTCCCCTATGGGGCAGGCGCCGGGGACCTGGAGTTCGTCAGGAGGAC CGTGGACTTCACCTCCCCACTCTTCAAGCCGGCGACTGGCTTCCCCCTTGGCTCCTCTCTCCGTGATTCC CTCTACTTCACAGACAATGGCCAGATCATCTTCCCAGAGTCAGACTACCAGATTTTCTCCTACCCCAACC CACTCCCAACAGGCTTCACAGGCCGGGACCCTGTGGCCCTGGTGGCTCCGTTCTGGGACGATGCTGACTT CTCCACTGGTCGGGGGACCACATTTTATCAGGAATACGAGACGTTCTATGGTGAACACAGCCTGCTAGTC CAGCAGGCCGAGTCTTGGATTAGAAAGATGACAAACAACGGGGGCTACAAGGCCAGGTGGGCCCTAAAGG TCACGTGGGTCAATGCCCACGCCTATCCTGCCCAGTGGACCCTCGGGAGCAACACCTACCAAGCCATCCT CTCCACGGACGGGAGCAGGTCCTATGCCCTGTTTCTCTACCAGAGCGGTGGGATGCAGTGGGACGTGGCC CAGCGCTCAGGCAACCCGGTGCTCATGGGCTTCTCTAGTGGAGATGGCTATTTCGAAAACAGCCCACTGA TGTCCCAGCCAGTGTGGGAGAGGTATCGCCCTGATAGATTCCTGAATTCCAACTCAGGCCTCCAAGGGCT GCAGTTCTACAGGCTACACCGGGAAGAAAGGCCCAACTACCGTCTCGAGTGCCTGCAGTGGCTGAAGAGC CAGCCTCGGTGGCCCAGCTGGGGCTGGAACCAGGTCTCCTGCCCTTGTTCCTGGCAGCAGGGACGACGGG ACTTACGATTCCAACCCGTCAGCATAGGTCGCTGGGGCCTCGGCAGTAGGCAGCTGTGCAGCTTCACCTC TTGGCGAGGAGGCGTGTGCTGCAGCTACGGGCCCTGGGGAGAGTTTCGTGAAGGCTGGCACGTGCAGCGT CCTTGGCAGTTGGCCCAGGAACTGGAGCCACAGAGCTGGTGCTGCCGCTGGAATGACAAGCCCTACCTCT GTGCCCTGTACCAGCAGAGGCGGCCCCACGTGGGCTGTGCTACATACAGGCCCCCACAGCCCGCCTGGAT GTTCGGGGACCCCCACATCACCACCTTGGATGGTGTCAGTTACACCTTCAATGGGCTGGGGGACTTCCTG CTGGTCGGGGCCCAAGACGGGAACTCCTCCTTCCTGCTTCAGGGCCGCACCGCCCAGACTGGCTCAGCCC AGGCCACCAACTTCATCGCCTTTGCGGCTCAGTACCGCTCCAGCAGCCTGGGCCCCGTCACGGTCCAATG GCTCCTTGAGCCTCACGACGCAATCCGTGTCCTGCTGGATAACCAGACTGTGACATTTCAGCCTGACCAT GAAGACGGCGGAGGCCAGGAGACGTTCAACGCCACCGGAGTCCTCCTGAGCCGCAACGGCTCTGAGGTCT CGGCCAGCTTCGACGGCTGGGCCACCGTCTCGGTGATCGCGCTCTCCAACATCCTCCACGCCTCCGCCAG CCTCCCGCCCGAGTACCAGAACCGCACGGAGGGGCTCCTGGGGGTCTGGAATAACAATCCAGAGGACGAC TTCAGGATGCCCAATGGCTCCACCATTCCCCCAGGGAGCCCTGAGGAGATGCTTTTCCACTTTGGAATGA CCTGGCAGATCAACGGGACAGGCCTCCTTGGCAAGAGGAATGACCAGCTGCCTTCCAACTTCACCCCTGT TTTCTACTCACAACTGCAAAAAAACAGCTCCTGGGCTGAACATTTGATCTCCAACTGTGACGGAGATAGC TCATGCATCTATGACACCCTGGCCCTGCGCAACGCAAGCATCGGACTTCACACGAGGGAAGTCAGTAAAA ACTACGAGCAGGCGAACGCCACCCTCAATCAGTACCCGCCCTCCATCAATGGTGGTCGTGTGATTGAAGC CTACAAGGGGCAGACCACGCTGATTCAGTACACCAGCAATGCTGAGGATGCCAACTTCACGCTCAGAGAC AGCTGCACCGACTTGGAGCTCTTTGAGAATGGGACGTTGCTGTGGACACCCAAGTCGCTGGAGCCATTCA CTCTGGAGATTCTAGCAAGAAGTGCCAAGATTGGCTTGGCATCTGCACTCCAGCCCAGGACTGTGGTCTG CCATTGCAATGCAGAGAGCCAGTGTTTGTACAATCAGACCAGCAGGGTGGGCAACTCCTCCCTGGAGGTG GCTGGCTGCAAGTGTGACGGGGGCACCTTCGGCCGCTACTGCGAGGGCTCCGAGGATGCCTGTGAGGAGC CGTGCTTCCCGAGTGTCCACTGCGTTCCTGGGAAGGGCTGCGAGGCCTGCCCTCCAAACCTGACTGGGGA TGGGCGGCACTGTGCGGCTCTGGGGAGCTCTTTCCTGTGTCAGAACCAGTCCTGCCCTGTGAATTACTGC TACAATCAAGGCCACTGCTACATCTCCCAGACTCTGGGCTGTCAGCCCATGTGCACCTGCCCCCCAGCCT TCACTGACAGCCGCTGCTTCCTGGCTGGGAACAACTTCAGTCCAACTGTCAACCTAGAACTTCCCTTAAG AGTCATCCAGCTCTTGCTCAGTGAAGAGGAAAATGCCTCCATGGCAGAAGTCAACGCCTCGGTGGCATAC AGACTGGGGACCCTGGACATGCGGGCCTTTCTCCGCAACAGCCAAGTGGAACGAATCGATTCTGCAGCAC CGGCCTCGGGAAGCCCCATCCAACACTGGATGGTCATCTCGGAGTTCCAGTACCGCCCTCGGGGCCCGGT CATTGACTTCCTGAACAACCAGCTGCTGGCCGCGGTGGTGGAGGCGTTCTTATACCACGTTCCACGGAGG AGTGAGGAGCCCAGGAACGACGTGGTCTTCCAGCCCATCTCCGGGGAAGACGTGCGCGATGTGACAGCCC TGAACGTGAGCACGCTGAAGGCTTACTTCAGATGCGATGGCTACAAGGGCTACGACCTGGTCTACAGCCC CCAGAGCGGCTTCACCTGCGTGTCCCCGTGCAGTAGGGGCTACTGTGACCATGGAGGCCAGTGCCAGCAC CTGCCCAGTGGGCCCCGCTGCAGCTGTGTGTCCTTCTCCATCTACACGGCCTGGGGCGAGCACTGTGAGC ACCTGAGCATGAAACTCGACGCGTTCTTCGGCATCTTCTTTGGGGCCCTGGGCGGCCTCTTGCTGCTGGG GGTCGGGACGTTCGTGGTCCTGCGCTTCTGGGGTTGCTCCGGGGCCAGGTTCTCCTATTTCCTGAACTCA GCTGAGGCCTTGCCT AGCGGACCGACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA >RG222229 representing NM_138297
Red=Cloning site Green=Tags(s) MKGARWRRVPWVSLSCLCLCLLPHVVPGVSLFPYGAGAGDLEFVRRTVDFTSPLFKPATGFPLGSSLRDS LYFTDNGQIIFPESDYQIFSYPNPLPTGFTGRDPVALVAPFWDDADFSTGRGTTFYQEYETFYGEHSLLV QQAESWIRKMTNNGGYKARWALKVTWVNAHAYPAQWTLGSNTYQAILSTDGSRSYALFLYQSGGMQWDVA QRSGNPVLMGFSSGDGYFENSPLMSQPVWERYRPDRFLNSNSGLQGLQFYRLHREERPNYRLECLQWLKS QPRWPSWGWNQVSCPCSWQQGRRDLRFQPVSIGRWGLGSRQLCSFTSWRGGVCCSYGPWGEFREGWHVQR PWQLAQELEPQSWCCRWNDKPYLCALYQQRRPHVGCATYRPPQPAWMFGDPHITTLDGVSYTFNGLGDFL LVGAQDGNSSFLLQGRTAQTGSAQATNFIAFAAQYRSSSLGPVTVQWLLEPHDAIRVLLDNQTVTFQPDH EDGGGQETFNATGVLLSRNGSEVSASFDGWATVSVIALSNILHASASLPPEYQNRTEGLLGVWNNNPEDD FRMPNGSTIPPGSPEEMLFHFGMTWQINGTGLLGKRNDQLPSNFTPVFYSQLQKNSSWAEHLISNCDGDS SCIYDTLALRNASIGLHTREVSKNYEQANATLNQYPPSINGGRVIEAYKGQTTLIQYTSNAEDANFTLRD SCTDLELFENGTLLWTPKSLEPFTLEILARSAKIGLASALQPRTVVCHCNAESQCLYNQTSRVGNSSLEV AGCKCDGGTFGRYCEGSEDACEEPCFPSVHCVPGKGCEACPPNLTGDGRHCAALGSSFLCQNQSCPVNYC YNQGHCYISQTLGCQPMCTCPPAFTDSRCFLAGNNFSPTVNLELPLRVIQLLLSEEENASMAEVNASVAY RLGTLDMRAFLRNSQVERIDSAAPASGSPIQHWMVISEFQYRPRGPVIDFLNNQLLAAVVEAFLYHVPRR SEEPRNDVVFQPISGEDVRDVTALNVSTLKAYFRCDGYKGYDLVYSPQSGFTCVSPCSRGYCDHGGQCQH LPSGPRCSCVSFSIYTAWGEHCEHLSMKLDAFFGIFFGALGGLLLLGVGTFVVLRFWGCSGARFSYFLNS AEALP SGPTRTRRLE - GFP Tag - V |
| Restriction Sites |
SgfI-RsrII
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_138297 |
| ORF Size | 3375 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_138297.5 |
| RefSeq Size | 3958 bp |
| RefSeq ORF | 3378 bp |
| Locus ID | 4585 |
| UniProt ID | Q99102 |
| Protein Families | Druggable Genome, Transmembrane |
| Gene Summary | The major constituents of mucus, the viscous secretion that covers epithelial surfaces such as those in the trachea, colon, and cervix, are highly glycosylated proteins called mucins. These glycoproteins play important roles in the protection of the epithelial cells and have been implicated in epithelial renewal and differentiation. This gene encodes an integral membrane glycoprotein found on the cell surface, although secreted isoforms may exist. At least two dozen transcript variants of this gene have been found, although for many of them the full-length transcript has not been determined or they are found only in tumor tissues. This gene contains a region in the coding sequence which has a variable number (>100) of 48 nt tandem repeats. [provided by RefSeq, Jul 2008] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC222229 | MUC4 (Myc-DDK-tagged)-Human mucin 4, cell surface associated (MUC4), transcript variant 5 |
CNY 8,616.00 |
|
| RC222229L3 | Lenti-ORF clone of MUC4 (Myc-DDK-tagged)-Human mucin 4, cell surface associated (MUC4), transcript variant 5 |
CNY 13,110.00 |
|
| RC222229L4 | Lenti-ORF clone of MUC4 (mGFP-tagged)-Human mucin 4, cell surface associated (MUC4), transcript variant 5 |
CNY 13,110.00 |
|
| SC306003 | MUC4 (untagged)-Human mucin 4, cell surface associated (MUC4), transcript variant 5 |
CNY 21,760.00 |
