PPP2R2B (NM_181677) Human Tagged ORF Clone
CAT#: RG222637
- TrueORF®
PPP2R2B (tGFP-tagged) - Human protein phosphatase 2, regulatory subunit B, beta (PPP2R2B), transcript variant 5
ORF Plasmid: DDK
"NM_181677" in other vectors (4)
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CNY 4,370.00
CNY 1,000.00
Specifications
Product Data | |
Type | Human Tagged ORF Clone |
Tag | TurboGFP |
Synonyms | B55BETA; PP2AB55BETA; PP2ABBETA; PP2APR55B; PP2APR55BETA; PR2AB55BETA; PR2ABBETA; PR2APR55BETA; PR52B; PR55-BETA; PR55BETA; SCA12 |
Vector | pCMV6-AC-GFP |
E. coli Selection | Ampicillin (100 ug/mL) |
Mammalian Cell Selection | Neomycin |
Sequence Data |
>RG222637 representing NM_181677
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGCAGAACTCTGACATTATCTCTACGGTAGAATTCAACCACACGGGAGAATTACTAGCGACAGGGGACA AGGGGGGTCGGGTTGTAATATTTCAACGAGAGCAGGAGAGTAAAAATCAGGTTCATCGTAGGGGTGAATA CAATGTTTACAGCACATTCCAGAGCCATGAACCCGAGTTCGATTACCTGAAGAGTTTAGAAATAGAAGAA AAAATCAATAAAATAAGATGGCTCCCCCAGCAGAATGCAGCTTACTTTCTTCTGTCTACTAATGATAAAA CTGTGAAGCTGTGGAAAGTCAGCGAGCGTGATAAGAGGCCAGAAGGCTACAATCTGAAAGATGAGGAGGG CCGGCTCCGGGATCCTGCCACCATCACAACCCTGCGGGTGCCTGTCCTGAGACCCATGGACCTGATGGTG GAGGCCACCCCACGAAGAGTATTTGCCAACGCACACACATATCACATCAACTCCATATCTGTCAACAGCG ACTATGAAACCTACATGTCCGCTGATGACCTGAGGATTAACCTATGGAACTTTGAAATAACCAATCAAAG TTTTAATATTGTGGACATTAAGCCAGCCAACATGGAGGAGCTCACGGAGGTGATCACAGCAGCCGAGTTC CACCCCCATCATTGCAACACCTTCGTGTACAGCAGCAGCAAAGGGACAATCCGGCTGTGTGACATGCGGG CATCTGCCCTGTGTGACAGGCACACCAAATTTTTTGAAGAGCCGGAAGATCCAAGCAACAGATCATTTTT CTCTGAAATTATCTCTTCGATTTCGGATGTGAAGTTCAGCCACAGTGGGAGGTATATCATGACCAGGGAC TACTTGACCGTCAAAGTCTGGGATCTCAACATGGAAAACCGCCCCATCGAGACTTACCAGGTTCATGACT ACCTCCGCAGCAAGCTGTGTTCCCTCTATGAAAATGACTGCATTTTTGATAAATTTGAGTGTGTGTGGAA TGGGTCAGACAGTGTCATCATGACAGGCTCCTACAACAACTTCTTCAGGATGTTCGACAGAAACACCAAG CGTGATGTGACCCTTGAGGCTTCGAGGGAAAACAGCAAGCCCCGGGCTATCCTCAAACCCCGAAAAGTGT GTGTGGGGGGCAAGCGGAGAAAAGACGAGATCAGTGTCGACAGTCTGGACTTTAGCAAAAAGATCTTGCA TACAGCTTGGCATCCTTCAGAAAATATTATAGCAGTGGCGGCTACAAATAACCTATATATATTCCAGGAC AAGGTTAAC ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA >RG222637 representing NM_181677
Red=Cloning site Green=Tags(s) MQNSDIISTVEFNHTGELLATGDKGGRVVIFQREQESKNQVHRRGEYNVYSTFQSHEPEFDYLKSLEIEE KINKIRWLPQQNAAYFLLSTNDKTVKLWKVSERDKRPEGYNLKDEEGRLRDPATITTLRVPVLRPMDLMV EATPRRVFANAHTYHINSISVNSDYETYMSADDLRINLWNFEITNQSFNIVDIKPANMEELTEVITAAEF HPHHCNTFVYSSSKGTIRLCDMRASALCDRHTKFFEEPEDPSNRSFFSEIISSISDVKFSHSGRYIMTRD YLTVKVWDLNMENRPIETYQVHDYLRSKLCSLYENDCIFDKFECVWNGSDSVIMTGSYNNFFRMFDRNTK RDVTLEASRENSKPRAILKPRKVCVGGKRRKDEISVDSLDFSKKILHTAWHPSENIIAVAATNNLYIFQD KVN TRTRPLE - GFP Tag - V |
Restriction Sites | SgfI-MluI Cloning Scheme for this gene Plasmid Map |
ACCN | NM_181677 |
ORF Size | 1269 bp |
OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
Reference Data | |
RefSeq | NM_181677.2, NP_858063.1 |
RefSeq Size | 2007 bp |
RefSeq ORF | 1272 bp |
Locus ID | 5521 |
UniProt ID | Q00005 |
Protein Families | Druggable Genome, Phosphatase |
Protein Pathways | Tight junction |
Gene Summary | The product of this gene belongs to the phosphatase 2 regulatory subunit B family. Protein phosphatase 2 is one of the four major Ser/Thr phosphatases, and it is implicated in the negative control of cell growth and division. It consists of a common heteromeric core enzyme, which is composed of a catalytic subunit and a constant regulatory subunit, that associates with a variety of regulatory subunits. The B regulatory subunit might modulate substrate selectivity and catalytic activity. This gene encodes a beta isoform of the regulatory subunit B55 subfamily. Defects in this gene cause autosomal dominant spinocerebellar ataxia 12 (SCA12), a disease caused by degeneration of the cerebellum, sometimes involving the brainstem and spinal cord, and in resulting in poor coordination of speech and body movements. Multiple alternatively spliced variants, which encode different isoforms, have been identified for this gene. The 5' UTR of some of these variants includes a CAG trinucleotide repeat sequence (7-28 copies) that can be expanded to 55-78 copies in cases of SCA12. [provided by RefSeq, Jul 2016] |
Documents
Product Manuals |
FAQs |
SDS |
Resources
Other Versions
SKU | Description | Size | Price |
---|---|---|---|
RC222637 | PPP2R2B (Myc-DDK-tagged)-Human protein phosphatase 2, regulatory subunit B, beta (PPP2R2B), transcript variant 5 |
CNY 3,656.00 |
|
RC222637L3 | Lenti-ORF clone of PPP2R2B (Myc-DDK-tagged)-Human protein phosphatase 2, regulatory subunit B, beta (PPP2R2B), transcript variant 5 |
CNY 5,890.00 |
|
RC222637L4 | Lenti-ORF clone of PPP2R2B (mGFP-tagged)-Human protein phosphatase 2, regulatory subunit B, beta (PPP2R2B), transcript variant 5 |
CNY 5,890.00 |
|
SC307342 | PPP2R2B (untagged)-Human protein phosphatase 2, regulatory subunit B, beta (PPP2R2B), transcript variant 5 |
CNY 6,840.00 |
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