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MTGR1 (CBFA2T2) (NM_005093) Human Tagged ORF Clone
CAT＃: RG222716
- TrueORF®
CBFA2T2 (tGFP-tagged) - Human core-binding factor, runt domain, alpha subunit 2; translocated to, 2 (CBFA2T2), transcript variant 2
ORF Plasmid: DDK
"NM_005093" in other vectors (4)
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CNY 5,040.00
Product images
CNY 6,281.00
Specifications
| Product Data | |
| Type | Human Tagged ORF Clone |
| Tag | TurboGFP |
| Synonyms | EHT; MTGR1; p85; ZMYND3 |
| Vector | pCMV6-AC-GFP |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>RG222716 representing NM_005093
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGCTAAAGAATCTGGAATAAGCTTGAAAGAAATACAGGTCCTGGCAAGGCAATGGAAAGTTGGTCCTG AGAAAAGGGTGCCAGCGATGCCTGGATCGCCTGTGGAAGTGAAGATACAGTCCAGATCCTCACCTCCCAC CATGCCACCCCTCCCACCAATAAATCCTGGAGGACCGAGGCCAGTGTCCTTCACTCCTACTGCATTAAGC AATGGCATCAACCATTCTCCTCCTACCCTGAATGGTGCCCCATCACCGCCACAGAGATTCAGCAATGGTC CTGCCTCCTCCACATCATCTGCACTCACAAATCAGCAATTGCCAGCCACTTGTGGTGCTCGACAACTCAG CAAGTTGAAACGCTTTCTTACCACTCTGCAACAGTTTGGCAATGACATCTCCCCTGAGATTGGGGAGAAG GTGCGGACTCTTGTTCTTGCACTGGTGAACTCAACAGTGACAATTGAGGAATTCCACTGTAAGCTCCAAG AAGCCACAAACTTTCCCCTTCGTCCTTTTGTGATTCCATTTCTCAAGGCCAACCTGCCCCTGCTGCAGCG GGAACTGCTGCACTGCGCTCGGGCGGCCAAGCAGACCCCATCCCAGTACCTGGCTCAGCACGAACACCTT CTGCTCAACACAAGCATTGCATCGCCTGCTGACTCGTCAGAGTTGCTCATGGAGGTGCACGGAAATGGGA AGAGGCCCAGTCCAGAGAGGAGAGAAGAGAATAGTTTTGATAGAGACACAATTGCTCCTGAGCCTCCTGC CAAGAGAGTATGTACCATCAGCCCTGCTCCTCGGCACAGTCCTGCTCTCACTGTGCCCCTCATGAATCCC GGGGGCCAATTCCATCCTACCCCTCCACCTCTTCAGCATTACACCTTAGAGGATATTGCAACTTCTCACC TGTATCGGGAACCCAACAAGATGCTAGAGCATCGAGAAGTTCGTGATAGACACCACAGTCTTGGTCTAAA TGGAGGCTATCAAGATGAGTTGGTAGATCATCGTTTGACAGAAAGGGAATGGGCTGATGAATGGAAACAT CTTGACCATGCGCTGAATTGCATTATGGAAATGGTAGAGAAAACAAGGCGCTCTATGGCAGTTCTGCGGC GCTGTCAGGAATCAGATCGTGAAGAACTCAACTACTGGAAAAGACGGTACAATGAAAACACAGAGCTGAG GAAAACGGGGACCGAGTTGGTCTCCAGGCAGCACAGCCCTGGGAGTGCAGATTCTCTCAGCAATGATTCT CAGAGAGAGTTCAACAGCAGGCCAGGTACAGGATACGTACCTGTGGAGTTTTGGAAAAAAACAGAAGAAG CTGTGAATAAGGTGAAAATTCAGGCCATGTCAGAAGTACAGAAGGCCGTCGCTGAGGCAGAGCAGAAAGC CTTTGAAGTGATTGCAACAGAGAGAGCACGAATGGAGCAAACCATAGCGGATGTCAAGCGGCAGGCCGCA GAGGATGCTTTCCTCGTCATCAATGAGCAAGAGGAGTCCACGGAGAACTGCTGGAACTGTGGCCGCAAAG CCAGCGAGACATGCAGTGGCTGCAATATCGCGCGATACTGTGGCTCTTTCTGCCAGCACAAGGACTGGGA GCGGCACCACCGCCTCTGTGGTCAGAACCTGCATGGCCAGAGCCCCCACGGCCAGGGCCGGCCGCTGCTT CCTGTAGGCAGGGGCTCCTCTGCCAGGTCCGCCGACTGCAGCGTGCCCAGCCCAGCCCTCGACAAGACCT CGGCAACCACATCGCGTTCCTCAACACCTGCTTCTGTGACAGCTATCGACACCAACGGACTC ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA >RG222716 representing NM_005093
Red=Cloning site Green=Tags(s) MAKESGISLKEIQVLARQWKVGPEKRVPAMPGSPVEVKIQSRSSPPTMPPLPPINPGGPRPVSFTPTALS NGINHSPPTLNGAPSPPQRFSNGPASSTSSALTNQQLPATCGARQLSKLKRFLTTLQQFGNDISPEIGEK VRTLVLALVNSTVTIEEFHCKLQEATNFPLRPFVIPFLKANLPLLQRELLHCARAAKQTPSQYLAQHEHL LLNTSIASPADSSELLMEVHGNGKRPSPERREENSFDRDTIAPEPPAKRVCTISPAPRHSPALTVPLMNP GGQFHPTPPPLQHYTLEDIATSHLYREPNKMLEHREVRDRHHSLGLNGGYQDELVDHRLTEREWADEWKH LDHALNCIMEMVEKTRRSMAVLRRCQESDREELNYWKRRYNENTELRKTGTELVSRQHSPGSADSLSNDS QREFNSRPGTGYVPVEFWKKTEEAVNKVKIQAMSEVQKAVAEAEQKAFEVIATERARMEQTIADVKRQAA EDAFLVINEQEESTENCWNCGRKASETCSGCNIARYCGSFCQHKDWERHHRLCGQNLHGQSPHGQGRPLL PVGRGSSARSADCSVPSPALDKTSATTSRSSTPASVTAIDTNGL TRTRPLE - GFP Tag - V |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_005093 |
| ORF Size | 1812 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_005093.3 |
| RefSeq Size | 6920 bp |
| RefSeq ORF | 1815 bp |
| Locus ID | 9139 |
| UniProt ID | O43439 |
| Domains | zf-MYND, TAFH |
| Protein Families | Transcription Factors |
| Gene Summary | In acute myeloid leukemia, especially in the M2 subtype, the t(8;21)(q22;q22) translocation is one of the most frequent karyotypic abnormalities. The translocation produces a chimeric gene made up of the 5'-region of the RUNX1 (AML1) gene fused to the 3'-region of the CBFA2T1 (MTG8) gene. The chimeric protein is thought to associate with the nuclear corepressor/histone deacetylase complex to block hematopoietic differentiation. The protein encoded by this gene binds to the AML1-MTG8 complex and may be important in promoting leukemogenesis. Several transcript variants are thought to exist for this gene, but the full-length natures of only three have been described. [provided by RefSeq, Jul 2008] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC222716 | CBFA2T2 (Myc-DDK-tagged)-Human core-binding factor, runt domain, alpha subunit 2, translocated to, 2 (CBFA2T2), transcript variant 2 |
CNY 4,496.00 |
|
| RC222716L3 | Lenti-ORF clone of CBFA2T2 (Myc-DDK-tagged)-Human core-binding factor, runt domain, alpha subunit 2; translocated to, 2 (CBFA2T2), transcript variant 2 |
CNY 6,460.00 |
|
| RC222716L4 | Lenti-ORF clone of CBFA2T2 (mGFP-tagged)-Human core-binding factor, runt domain, alpha subunit 2; translocated to, 2 (CBFA2T2), transcript variant 2 |
CNY 6,460.00 |
|
| SC314164 | CBFA2T2 (untagged)-Human core-binding factor, runt domain, alpha subunit 2, translocated to, 2 (CBFA2T2), transcript variant 2 |
CNY 9,790.00 |
