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AMPK gamma 3 (PRKAG3) (NM_017431) Human Tagged ORF Clone
CAT＃: RG222787
- TrueORF®
PRKAG3 (tGFP-tagged) - Human protein kinase, AMP-activated, gamma 3 non-catalytic subunit (PRKAG3)
ORF Plasmid: DDK
"NM_017431" in other vectors (6)
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CNY 5,256.00
Product images
CNY 1,000.00
热销推荐Specifications
| Product Data | |
| Type | Human Tagged ORF Clone |
| Tag | TurboGFP |
| Synonyms | AMPKG3; SMGMQTL |
| Vector | pCMV6-AC-GFP |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>RG222787 representing NM_017431
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGAGCCCGGGCTGGAGCACGCACTGCGCAGGACCCCTTCCTGGAGCAGCCTTGGGGGTTCTGAGCATC AAGAGATGAGCTTCCTAGAGCAAGAAAACAGCAGCTCATGGCCATCACCAGCTGTGACCAGCAGCTCAGA AAGAATCCGTGGGAAACGGAGGGCCAAAGCCTTGAGATGGACAAGGCAGAAGTCGGTGGAGGAAGGGGAG CCACCAGGTCAGGGGGAAGGTCCCCGGTCCAGGCCAGCTGCTGAGTCCACCGGGCTGGAGGCCACATTCC CCAAGACCACACCCTTGGCTCAAGCTGATCCTGCCGGGGTGGGCACTCCACCAACAGGGTGGGACTGCCT CCCCTCTGACTGTACAGCCTCAGCTGCAGGCTCCAGCACAGATGATGTGGAGCTGGCCACGGAGTTCCCA GCCACAGAGGCCTGGGAGTGTGAGCTAGAAGGCCTGCTGGAAGAGAGGCCTGCCCTGTGCCTGTCCCCGC AGGCCCCATTTCCCAAGCTGGGCTGGGATGACGAACTGCGGAAACCCGGCGCCCAGATCTACATGCGCTT CATGCAGGAGCACACCTGCTACGATGCCATGGCAACTAGCTCCAAGCTAGTCATCTTCGACACCATGCTG GAGATCAAGAAGGCCTTCTTTGCTCTGGTGGCCAACGGTGTGCGGGCAGCCCCTCTATGGGACAGCAAGA AGCAGAGCTTTGTGGGGATGCTGACCATCACTGACTTCATCCTGGTGCTGCATCGCTACTACAGGTCCCC CCTGGTCCAGATCTATGAGATTGAACAACATAAGATTGAGACCTGGAGGGAGATCTACCTGCAAGGCTGC TTCAAGCCTCTGGTCTCCATCTCTCCTAATGATAGCCTGTTTGAAGCTGTCTACACCCTCATCAAGAACC GGATCCATCGCCTGCCTGTTCTTGACCCGGTGTCAGGCAACGTACTCCACATCCTCACACACAAACGCCT GCTCAAGTTCCTGCACATCTTTGGTTCCCTGCTGCCCCGGCCCTCCTTCCTCTACCGCACTATCCAAGAT TTGGGCATCGGCACATTCCGAGACTTGGCTGTGGTGCTGGAGACAGCACCCATCCTGACTGCACTGGACA TCTTTGTGGACCGGCGTGTGTCTGCACTGCCTGTGGTCAACGAATGTGGTCAGGTCGTGGGCCTCTATTC CCGCTTTGATGTGATTCACCTGGCTGCCCAGCAAACCTACAACCACCTGGACATGAGTGTGGGAGAAGCC CTGAGGCAGAGGACACTATGTCTGGAGGGAGTCCTTTCCTGCCAGCCCCACGAGAGCTTGGGGGAAGTGA TCGACAGGATTGCTCGGGAGCAGGTACACAGGCTGGTGCTAGTGGACGAGACCCAGCATCTCTTGGGCGT GGTCTCCCTCTCCGACATCCTTCAGGCACTGGTGCTCAGCCCTGCTGGCATCGATGCCCTCGGGGCC ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA >RG222787 representing NM_017431
Red=Cloning site Green=Tags(s) MEPGLEHALRRTPSWSSLGGSEHQEMSFLEQENSSSWPSPAVTSSSERIRGKRRAKALRWTRQKSVEEGE PPGQGEGPRSRPAAESTGLEATFPKTTPLAQADPAGVGTPPTGWDCLPSDCTASAAGSSTDDVELATEFP ATEAWECELEGLLEERPALCLSPQAPFPKLGWDDELRKPGAQIYMRFMQEHTCYDAMATSSKLVIFDTML EIKKAFFALVANGVRAAPLWDSKKQSFVGMLTITDFILVLHRYYRSPLVQIYEIEQHKIETWREIYLQGC FKPLVSISPNDSLFEAVYTLIKNRIHRLPVLDPVSGNVLHILTHKRLLKFLHIFGSLLPRPSFLYRTIQD LGIGTFRDLAVVLETAPILTALDIFVDRRVSALPVVNECGQVVGLYSRFDVIHLAAQQTYNHLDMSVGEA LRQRTLCLEGVLSCQPHESLGEVIDRIAREQVHRLVLVDETQHLLGVVSLSDILQALVLSPAGIDALGA TRTRPLE - GFP Tag - V |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_017431 |
| ORF Size | 1467 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq | NM_017431.3 |
| RefSeq Size | 2299 bp |
| RefSeq ORF | 1470 bp |
| Locus ID | 53632 |
| UniProt ID | Q9UGI9 |
| Domains | CBS |
| Protein Families | Druggable Genome |
| Protein Pathways | Adipocytokine signaling pathway, Hypertrophic cardiomyopathy (HCM), Insulin signaling pathway |
| Gene Summary | The protein encoded by this gene is a regulatory subunit of the AMP-activated protein kinase (AMPK). AMPK is a heterotrimer consisting of an alpha catalytic subunit, and non-catalytic beta and gamma subunits. AMPK is an important energy-sensing enzyme that monitors cellular energy status. In response to cellular metabolic stresses, AMPK is activated, and thus phosphorylates and inactivates acetyl-CoA carboxylase (ACC) and beta-hydroxy beta-methylglutaryl-CoA reductase (HMGCR), key enzymes involved in regulating de novo biosynthesis of fatty acid and cholesterol. This subunit is one of the gamma regulatory subunits of AMPK. It is dominantly expressed in skeletal muscle. Studies of the pig counterpart suggest that this subunit may play a key role in the regulation of energy metabolism in skeletal muscle. [provided by RefSeq, Jul 2008] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC222787 | PRKAG3 (Myc-DDK-tagged)-Human protein kinase, AMP-activated, gamma 3 non-catalytic subunit (PRKAG3) |
CNY 3,656.00 |
|
| RC222787L1 | Lenti ORF clone of Human protein kinase, AMP-activated, gamma 3 non-catalytic subunit (PRKAG3), Myc-DDK-tagged |
CNY 6,056.00 |
|
| RC222787L2 | Lenti ORF clone of Human protein kinase, AMP-activated, gamma 3 non-catalytic subunit (PRKAG3), mGFP tagged |
CNY 6,056.00 |
|
| RC222787L3 | Lenti ORF clone of Human protein kinase, AMP-activated, gamma 3 non-catalytic subunit (PRKAG3), Myc-DDK-tagged |
CNY 6,056.00 |
|
| RC222787L4 | Lenti ORF clone of Human protein kinase, AMP-activated, gamma 3 non-catalytic subunit (PRKAG3), mGFP tagged |
CNY 6,056.00 |
|
| SC114107 | PRKAG3 (untagged)-Human protein kinase, AMP-activated, gamma 3 non-catalytic subunit (PRKAG3) |
CNY 3,656.00 |
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