PRAMEF20 (NM_001099852) Human Tagged ORF Clone
CAT#: RG223125
- TrueORF®
PRAMEF20 (tGFP-tagged) - Human PRAME family member 20 (PRAMEF20)
ORF Plasmid: DDK
"NM_001099852" in other vectors (4)
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CNY 4,370.00
Specifications
Product Data | |
Type | Human Tagged ORF Clone |
Tag | TurboGFP |
Synonyms | PRAMEF21 |
Vector | pCMV6-AC-GFP |
E. coli Selection | Ampicillin (100 ug/mL) |
Mammalian Cell Selection | Neomycin |
Sequence Data |
>RG223125 representing NM_001099852
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGAGCATCCGGACTCCACCCAGACTCCTGGAGCTGGCGGGGCGGAGCCTGCTGAGGGACGAGGCCTTGG CCATCTCCACCCTGGAGGAGCTGCCCACGGAACTTTTTCCCCCATTGTTCATGGAGGCCTTCAGCAGGAG ACACTGTGAGGCCCTGAAGCTGATGGTGCAGGCCTGGCCTTTCCTCCGCCTTCCTCTGGGGTCTCTGATG AAAAGGCCTTGCCCAGAGACCTTCCAAGCTGTGCTCGATGGGCTTGATGCACTGCTTACCCACAGGGTTC GTCTCAGGAGGTGGAAACTTCAAGTGCTGGATTTACAGGATGTCAGTGAGAACTTCTGGATGGTTTGGTC TGAAGCCATGGCCCGTAGGTGCTTACCAAATGCCATGATGAACAGAAAACCAGTGCAGGACTGTCCAAGG ATGAGAGGACAGCAGCCCTTGACTGTGTTCATAGACCTTTGCCTCAAGAACAGGACTCTGGATGAATACT TCACCTGCCTCTTTCTATGGGTCAAGCAGAGGGAAGGTTTAGTACACCTGTGCTGTAAGAAGCTGAAAAT GTTGGGAATGCTCTTCCACAATATCAGAAACATCCTGAAAACAGTCAACCTAGACTGTATCCAGGAGGTG GAAGTGAATTGCAATTGGACACTGCCCGTCCTGGCAGAGTTTACCCCATACCTCGGCCAGATGAGGAATC TTCGGAAGCTCGTTCTCTCTGACATAGATTCTCGCTACATTTCCCCAGAGCAGAAGAAGGAGTTTGTTAC CCAGTTCACCACTCAGTTCCTCAAGCTGCGCTGCCTCCAAAAGCTTTATATGAACTCTGTTTCTTTCCTC GAAGGCCACCTGGACCAGATGCTCAGCTGTCTAAAGACCTCGTTAAACATCCTCGCAATAACTAACTGTG TGCTTTTGGAATCAGACTTGAAGCATCTGTCCAAGTACCCGAGCATTGGTCAACTAAAGACCCTGGACCT GAGTGGCACCAGACTGGCCAATTTCAGCCTTGTGCCGCTCCAAGTTCTCCTAGAAAAAGTTGCAGCCACC CTTGAGTACCTGGACTTAGATGACTGTGGCATCGTAGACTCCCAAGTCAACGCCATCCTGCCTGCCCTGA GCCGCTGCTTTGAGCTCACCACCTTCAGCTTCCGTGGAAATCCCATCTCCACGGCCACCCTGGAGAACCT GCTGTGCCACACAATCAGACTCAACAACTTATGCCTGGAGCTGTATCCTGCCCCGCGGGAGAGTTATGAT GTTCGTGGTATCGTCTGCCGGAGCAGATTTGCCCAACTTGGGGCTGAGCTGATGGGGAGAGTGAGGGCCT TAAGGGAGCCCGAGAGGATCTTGTTCTGTACCGACTACTGCCCTCAGTGTGGCAACAGGTCACTTTACGA CCTGGAGGTAGATCGGTGTTGCTGT ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA >RG223125 representing NM_001099852
Red=Cloning site Green=Tags(s) MSIRTPPRLLELAGRSLLRDEALAISTLEELPTELFPPLFMEAFSRRHCEALKLMVQAWPFLRLPLGSLM KRPCPETFQAVLDGLDALLTHRVRLRRWKLQVLDLQDVSENFWMVWSEAMARRCLPNAMMNRKPVQDCPR MRGQQPLTVFIDLCLKNRTLDEYFTCLFLWVKQREGLVHLCCKKLKMLGMLFHNIRNILKTVNLDCIQEV EVNCNWTLPVLAEFTPYLGQMRNLRKLVLSDIDSRYISPEQKKEFVTQFTTQFLKLRCLQKLYMNSVSFL EGHLDQMLSCLKTSLNILAITNCVLLESDLKHLSKYPSIGQLKTLDLSGTRLANFSLVPLQVLLEKVAAT LEYLDLDDCGIVDSQVNAILPALSRCFELTTFSFRGNPISTATLENLLCHTIRLNNLCLELYPAPRESYD VRGIVCRSRFAQLGAELMGRVRALREPERILFCTDYCPQCGNRSLYDLEVDRCCC TRTRPLE - GFP Tag - V |
Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
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ACCN | NM_001099852 |
ORF Size | 1425 bp |
OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
Reference Data | |
RefSeq | NM_001099852.1, NP_001093322.1 |
RefSeq Size | 1597 bp |
RefSeq ORF | 1428 bp |
Locus ID | 645425 |
UniProt ID | Q5VT98 |
Documents
Product Manuals |
FAQs |
SDS |
Resources
Other Versions
SKU | Description | Size | Price |
---|---|---|---|
RC223125 | PRAMEF20 (Myc-DDK-tagged)-Human PRAME family member 20 (PRAMEF20) |
CNY 3,656.00 |
|
RC223125L3 | Lenti ORF clone of Human PRAME family member 20 (PRAMEF20), Myc-DDK-tagged |
CNY 5,890.00 |
|
RC223125L4 | Lenti ORF clone of Human PRAME family member 20 (PRAMEF20), mGFP tagged |
CNY 5,890.00 |
|
SC316761 | PRAMEF20 (untagged)-Human PRAME family member 20 (PRAMEF20) |
CNY 7,510.00 |
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