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PRAMEF20 (NM_001099852) Human Untagged Clone
CAT＃: SC316761
PRAMEF20 (untagged)-Human PRAME family member 20 (PRAMEF20)
CNY 7,510.00
Product images
Specifications
| Product Data | |
| Type | Human Untagged Clone |
| Tag | Tag Free |
| Synonyms | PRAMEF21 |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>SC316761 representing NM_001099852.
Blue=Insert sequence Red=Cloning site Green=Tag(s) GCTCGTTTAGTGAACCGTCAGAATTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTG GATCCGGTACCGAGGAGATCTGCCGCCGCGATCGCC ATGAGCATCCGGACTCCACCCAGACTCCTGGAGCTGGCGGGGCGGAGCCTGCTGAGGGACGAGGCCTTG GCCATCTCCACCCTGGAGGAGCTGCCCACGGAACTTTTTCCCCCATTGTTCATGGAGGCCTTCAGCAGG AGACACTGTGAGGCCCTGAAGCTGATGGTGCAGGCCTGGCCTTTCCTCCGCCTTCCTCTGGGGTCTCTG ATGAAAAGGCCTTGCCCAGAGACCTTCCAAGCTGTGCTCGATGGGCTTGATGCACTGCTTACCCACAGG GTTCGTCTCAGGAGGTGGAAACTTCAAGTGCTGGATTTACAGGATGTCAGTGAGAACTTCTGGATGGTT TGGTCTGAAGCCATGGCCCGTAGGTGCTTACCAAATGCCATGATGAACAGAAAACCACTGCAGGACTGT CCAAGGATGAGAGGACAGCAGCCCTTGACTGTGTTCATAGACCTTTGCCTCAAGAACAGGACTCTGGAT GAATACTTCACCTGCCTCTTTCTATGGGTCAAGCAGAGGGAAGGTTTAGTACACCTGTGCTGTAAGAAG CTGAAAATGTTGGGAATGCTCTTCCACAATATCAGAAACATCCTGAAAACAGTCAACCTAGACTGTATC CAGGAGGTGGAAGTGAATTGCAATTGGACACTGCCCGTCCTGGCAGAGTTTACCCCATACCTCGGCCAG ATGAGGAATCTTCGGAAGCTCGTTCTCTCTGACATAGATTCTCGCTACATTTCCCCAGAGCAGAAGAAG GAGTTTGTTACCCAGTTCACCACTCAGTTCCTCAAGCTGCGCTGCCTCCAAAAGCTTTATATGAACTCT GTTTCTTTCCTCGAAGGCCACCTGGACCAGATGCTCAGCTGTCTAAAGACCTCGTTAAACATCCTCGCA ATAACTAACTGTGTGCTTTTGGAATCAGACTTGAAGCATCTGTCCAAGTACCCGAGCATTGGTCAACTA AAGACCCTGGACCTGAGTGGCACCAGACTGGCCAATTTCAGCCTTGTGCCGCTCCAAGTTCTCCTAGAA AAAGTTGCAGCCACCCTTGAGTACCTGGACTTAGATGACTGTGGCATCGTAGACTCCCAAGTCAACGCC ATCCTGCCTGCCCTGAGCCGCTGCTTTGAGCTCACCACCTTCAGCTTCCGTGGAAATCCCATCTCCACG GCCACCCTGGAGAACCTGCTGTGCCACACAATCAGACTCAACAACTTATGCCTGGAGCTGTATCCTGCC CCGCGGGAGAGTTATGATGTTCGTGGTATCGTCTGCCGGAGCAGATTTGCCCAACTTGGGGCTGAGCTG ATGGGGAGAGTGAGGGCCTTAAGGGAGCCCGAGAGGATCTTGTTCTGTACCGACTACTGCCCTCAGTGT GGCAACAGGTCACTTTACGACCTGGAGGTAGATCGGTGTTGCTGTTGA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGAT TACAAGGATGACGACGATAAGGTTTAAACGGCCGGC |
| Restriction Sites | SgfI-MluI |
| ACCN | NM_001099852 |
| Insert Size | 1428 bp |
| OTI Disclaimer | Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP). |
| OTI Annotation | This TrueClone is provided through our Custom Cloning Process that includes sub-cloning into OriGene's pCMV6 vector and full sequencing to provide a non-variant match to the expected reference without frameshifts, and is delivered as lyophilized plasmid DNA. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq | NM_001099852.2 |
| RefSeq Size | 1597 bp |
| RefSeq ORF | 1428 bp |
| Locus ID | 645425 |
| UniProt ID | Q5VT98 |
| MW | 54.8 kDa |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC223125 | PRAMEF20 (Myc-DDK-tagged)-Human PRAME family member 20 (PRAMEF20) |
CNY 3,656.00 |
|
| RC223125L3 | Lenti ORF clone of Human PRAME family member 20 (PRAMEF20), Myc-DDK-tagged |
CNY 5,890.00 |
|
| RC223125L4 | Lenti ORF clone of Human PRAME family member 20 (PRAMEF20), mGFP tagged |
CNY 5,890.00 |
|
| RG223125 | PRAMEF20 (tGFP-tagged) - Human PRAME family member 20 (PRAMEF20) |
CNY 4,370.00 |
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