PUF60 (NM_078480) Human Tagged ORF Clone
CAT#: RG223492
- TrueORF®
PUF60 (tGFP-tagged) - Human poly-U binding splicing factor 60KDa (PUF60), transcript variant 1
ORF Plasmid: DDK
"NM_078480" in other vectors (4)
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CNY 5,040.00
Specifications
Product Data | |
Type | Human Tagged ORF Clone |
Tag | TurboGFP |
Synonyms | FIR; RoBPI; SIAHBP1; VRJS |
Vector | pCMV6-AC-GFP |
E. coli Selection | Ampicillin (100 ug/mL) |
Mammalian Cell Selection | Neomycin |
Sequence Data |
>RG223492 representing NM_078480
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGCGACGGCGACCATAGCTCTCCAGGTCAATGGCCAGCAAGGAGGGGGGTCCGAGCCGGCGGCGGCGG CGGCAGTGGTGGCAGCGGGAGACAAATGGAAACCTCCACAGGGCACAGACTCCATCAAGATGGAGAACGG GCAGAGCACAGCCGCCAAGCTGGGGCTGCCTCCCCTGACGCCCGAGCAGCAGGAGGCCCTTCAGAAGGCC AAGAAGTACGCCATGGAGCAGAGCATCAAGAGTGTGCTGGTGAAGCAGACCATCGCGCACCAGCAGCAGC AGCTCACCAACCTGCAGATGGCAGCAGTGACAATGGGCTTTGGAGATCCTCTCTCACCTTTGCAATCGAT GGCGGCTCAGCGGCAGCGGGCGCTGGCCATCATGTGCCGCGTCTACGTGGGCTCTATCTACTATGAGCTG GGGGAGGACACCATCCGCCAGGCCTTTGCCCCCTTTGGCCCCATCAAGAGCATCGACATGTCCTGGGACT CCGTCACCATGAAGCACAAGGGCTTTGCCTTCGTGGAGTATGAGGTCCCCGAAGCTGCACAGCTGGCCTT GGAGCAGATGAACTCGGTGATGCTGGGGGGCAGGAACATCAAGGTGGGCAGACCCAGCAACATAGGGCAG GCCCAGCCCATCATAGACCAGTTGGCTGAGGAGGCACGGGCCTTCAACCGCATCTACGTGGCCTCTGTGC ACCAGGACCTCTCAGACGATGACATCAAGAGCGTGTTTGAGGCCTTTGGCAAGATCAAGTCCTGCACACT GGCCCGGGACCCCACAACTGGCAAGCACAAGGGCTACGGCTTCATTGAGTACGAGAAGGCCCAGTCGTCC CAAGATGCTGTGTCTTCCATGAACCTCTTTGACCTGGGTGGCCAGTACTTGCGGGTGGGCAAGGCTGTCA CACCGCCCATGCCCCTACTCACACCAGCCACGCCTGGAGGCCTCCCACCTGCCGCTGCTGTGGCAGCTGC TGCAGCCACTGCCAAGATCACAGCTCAGGAAGCAGTGGCCGGAGCAGCGGTGCTGGGTACCCTGGGCACA CCTGGACTGGTGTCCCCAGCACTGACCCTGGCCCAGCCCCTGGGCACTTTGCCCCAGGCTGTCATGGCTG CCCAGGCACCTGGAGTCATCACAGGTGTGACCCCAGCCCGTCCTCCTATCCCGGTCACCATCCCCTCGGT GGGAGTGGTGAACCCCATCCTGGCCAGCCCTCCAACGCTGGGTCTCCTGGAGCCCAAGAAGGAGAAGGAA GAAGAGGAGCTGTTTCCCGAGTCAGAGCGGCCAGAGATGCTGAGCGAGCAGGAGCACATGAGCATCTCGG GCAGTAGCGCCCGACACATGGTGATGCAGAAGCTGCTCCGCAAGCAGGAGTCTACAGTGATGGTTCTGCG CAACATGGTGGACCCCAAGGACATCGATGATGACCTGGAAGGGGAGGTGACAGAGGAGTGTGGCAAGTTC GGGGCCGTGAACCGCGTCATCATCTACCAAGAGAAACAAGGCGAGGAGGAGGATGCAGAAATCATTGTCA AGATCTTTGTGGAGTTTTCCATAGCCTCTGAGACTCATAAGGCCATCCAGGCCCTCAATGGCCGCTGGTT TGCTGGCCGCAAGGTGGTGGCTGAAGTGTACGACCAGGAGCGTTTTGATAACAGTGACCTCTCTGCG ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA >RG223492 representing NM_078480
Red=Cloning site Green=Tags(s) MATATIALQVNGQQGGGSEPAAAAAVVAAGDKWKPPQGTDSIKMENGQSTAAKLGLPPLTPEQQEALQKA KKYAMEQSIKSVLVKQTIAHQQQQLTNLQMAAVTMGFGDPLSPLQSMAAQRQRALAIMCRVYVGSIYYEL GEDTIRQAFAPFGPIKSIDMSWDSVTMKHKGFAFVEYEVPEAAQLALEQMNSVMLGGRNIKVGRPSNIGQ AQPIIDQLAEEARAFNRIYVASVHQDLSDDDIKSVFEAFGKIKSCTLARDPTTGKHKGYGFIEYEKAQSS QDAVSSMNLFDLGGQYLRVGKAVTPPMPLLTPATPGGLPPAAAVAAAAATAKITAQEAVAGAAVLGTLGT PGLVSPALTLAQPLGTLPQAVMAAQAPGVITGVTPARPPIPVTIPSVGVVNPILASPPTLGLLEPKKEKE EEELFPESERPEMLSEQEHMSISGSSARHMVMQKLLRKQESTVMVLRNMVDPKDIDDDLEGEVTEECGKF GAVNRVIIYQEKQGEEEDAEIIVKIFVEFSIASETHKAIQALNGRWFAGRKVVAEVYDQERFDNSDLSA TRTRPLE - GFP Tag - V |
Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
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ACCN | NM_078480 |
ORF Size | 1677 bp |
OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
Reference Data | |
RefSeq | NM_078480.3 |
RefSeq Size | 1946 bp |
RefSeq ORF | 1680 bp |
Locus ID | 22827 |
UniProt ID | Q9UHX1 |
Domains | RRM, RRM_1 |
Protein Pathways | Spliceosome |
Gene Summary | This gene encodes a nucleic acid-binding protein that plays a role in a variety of nuclear processes, including pre-mRNA splicing and transcriptional regulation. The encoded protein forms a complex with the far upstream DNA element (FUSE) and FUSE-binding protein at the myelocytomatosis oncogene (MYC) promoter. This complex represses MYC transcription through the core-TFIIH basal transcription factor. Alternatively spliced transcript variants encoding multiple isoforms have been observed for this gene. [provided by RefSeq, Aug 2012] |
Documents
Product Manuals |
FAQs |
SDS |
Resources
Other Versions
SKU | Description | Size | Price |
---|---|---|---|
RC223492 | PUF60 (Myc-DDK-tagged)-Human poly-U binding splicing factor 60KDa (PUF60), transcript variant 1 |
CNY 4,152.00 |
|
RC223492L3 | Lenti ORF clone of Human poly-U binding splicing factor 60KDa (PUF60), transcript variant 1, Myc-DDK-tagged |
CNY 6,460.00 |
|
RC223492L4 | Lenti ORF clone of Human poly-U binding splicing factor 60KDa (PUF60), transcript variant 1, mGFP tagged |
CNY 6,460.00 |
|
SC110270 | PUF60 (untagged)-Human poly-U binding splicing factor 60KDa (PUF60), transcript variant 1 |
CNY 4,168.00 |