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KRT25 (NM_181534) Human Tagged ORF Clone
CAT＃: RG223582
- TrueORF®
KRT25 (tGFP-tagged) - Human keratin 25 (KRT25)
ORF Plasmid: DDK
"NM_181534" in other vectors (4)
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CNY 4,370.00
Product images
CNY 1,000.00
Specifications
| Product Data | |
| Type | Human Tagged ORF Clone |
| Tag | TurboGFP |
| Synonyms | ARWH3; KRT24IRS1; KRT25A |
| Vector | pCMV6-AC-GFP |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>RG223582 representing NM_181534
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGTCTCTTCGACTTTCCAGTGCATCCAGGAGGTCCTGTCCTCGTCCCACCACTGGATCACTCAGACTCT ATGGTGGGGGAACCAGCTTTGGTACTGGAAATTCTTGTGGCATTTCAGGGATTGGAAGTGGCTTCTCTAG TGCCTTCGGAGGCAGCTCATCGGGAGGAAACACAGGGGGAGGTAATCCCTGTGCTGGCTTCACTGTGAAT GAGCGGGGGCTCCTTTCTGGCAATGAGAAGGTGACCATGCAGAACCTCAATGACCGCCTGGCATCCTACC TGGACAGTGTGCATGCTCTGGAGGAGGCCAACGCTGACCTGGAGCAGAAGATCAAGGGCTGGTATGAGAA ATTTGGGCCTGGCTCTTGCCGTGGTCTTGATCATGACTATAGCAGATATTTCCCAATAATTGATGACCTT AAAAATCAGATCATCGCATCCACCACCAGCAATGCTAATGCTGTTCTGCAGATCGATAATGCCAGGCTTA CAGCTGATGATTTCAGACTCAAGTATGAAAATGAGCTGGCTCTTCACCAGAGTGTAGAGGCTGATGTCAA TGGGTTACGAAGAGTTTTGGATGAAATAACCCTGTGCAGAACAGATCTGGAGATTCAGTATGAAACCCTG AGTGAGGAGATGACTTACCTCAAAAAGAACCATAAAGAGGAAATGCAAGTTCTGCAGTGCGCAGCTGGAG GCAACGTGAACGTGGAGATGAACGCAGCCCCCGGGGTGGACCTCACAGTTCTGCTGAACAACATGCGAGC TGAGTACGAAGCCCTTGCAGAGCAGAACCGCAGGGACGCGGAGGCCTGGTTCAACGAGAAGAGCGCCTCC CTGCAGCAGCAGATCTCTGAGGATGTCGGAGCCACAACCTCAGCCCGGAATGAGCTGACTGAAATGAAGC GCACTCTTCAAACCCTGGAAATTGAACTTCAGTCTCTCCTAGCCACGAAACACTCCCTGGAGTGCTCCTT GACAGAGACCGAGAGCAACTACTGTGCGCAGCTGGCGCAGATCCAGGCTCAGATCGGGGCCCTGGAGGAG CAGCTGCACCAGGTCAGAACCGAGACCGAGGGCCAGAAGCTGGAGTATGAGCAGCTCCTGGACATCAAGC TCCACCTGGAAAAAGAAATTGAGACCTACTGTCTCCTTATAGGAGGAGATGATGGAGCCTGTAAGTCTGG GGGTTACAAGTCTAAAGATTATGGATCTGGAAATGTGGGAAGTCAAGTCAAAGACCCAGCCAAAGCCATA GTGGTTAAGAAAGTTCTTGAGGAGGTAGACCAACGCAGCAAAATACTTACCACCAGGCTCCACTCCCTGG AAGAGAAATCTCAAAGCAAT AGCGGACCGACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA >RG223582 representing NM_181534
Red=Cloning site Green=Tags(s) MSLRLSSASRRSCPRPTTGSLRLYGGGTSFGTGNSCGISGIGSGFSSAFGGSSSGGNTGGGNPCAGFTVN ERGLLSGNEKVTMQNLNDRLASYLDSVHALEEANADLEQKIKGWYEKFGPGSCRGLDHDYSRYFPIIDDL KNQIIASTTSNANAVLQIDNARLTADDFRLKYENELALHQSVEADVNGLRRVLDEITLCRTDLEIQYETL SEEMTYLKKNHKEEMQVLQCAAGGNVNVEMNAAPGVDLTVLLNNMRAEYEALAEQNRRDAEAWFNEKSAS LQQQISEDVGATTSARNELTEMKRTLQTLEIELQSLLATKHSLECSLTETESNYCAQLAQIQAQIGALEE QLHQVRTETEGQKLEYEQLLDIKLHLEKEIETYCLLIGGDDGACKSGGYKSKDYGSGNVGSQVKDPAKAI VVKKVLEEVDQRSKILTTRLHSLEEKSQSN SGPTRTRRLE - GFP Tag - V |
| Restriction Sites |
SgfI-RsrII
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_181534 |
| ORF Size | 1350 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq | NM_181534.4 |
| RefSeq Size | 1684 bp |
| RefSeq ORF | 1353 bp |
| Locus ID | 147183 |
| UniProt ID | Q7Z3Z0 |
| Gene Summary | This gene encodes a member of the type I (acidic) keratin family, which belongs to the superfamily of intermediate filament (IF) proteins. Keratins are heteropolymeric structural proteins which form the intermediate filament. These filaments, along with actin microfilaments and microtubules, compose the cytoskeleton of epithelial cells. The type I keratin genes are clustered in a region of chromosome 17q12-q21. [provided by RefSeq, Jul 2009] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC223582 | KRT25 (Myc-DDK-tagged)-Human keratin 25 (KRT25) |
CNY 3,656.00 |
|
| RC223582L3 | Lenti ORF clone of Human keratin 25 (KRT25), Myc-DDK-tagged |
CNY 5,890.00 |
|
| RC223582L4 | Lenti ORF clone of Human keratin 25 (KRT25), mGFP tagged |
CNY 5,890.00 |
|
| SC307301 | KRT25 (untagged)-Human keratin 25 (KRT25) |
CNY 7,220.00 |
