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TORC1 (CRTC1) (NM_001098482) Human Tagged ORF Clone
CAT＃: RG224693
- TrueORF®
CRTC1 (tGFP-tagged) - Human CREB regulated transcription coactivator 1 (CRTC1), transcript variant 3
ORF Plasmid: DDK
"NM_001098482" in other vectors (4)
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CNY 6,270.00
Product images
Specifications
| Product Data | |
| Type | Human Tagged ORF Clone |
| Tag | TurboGFP |
| Synonyms | Mam-2; MAML2; MECT1; TORC-1; TORC1; WAMTP1 |
| Vector | pCMV6-AC-GFP |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>RG224693 representing NM_001098482
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGCGACTTCGAACAATCCGCGGAAATTCAGCGAGAAGATCGCGCTGCACAATCAGAAGCAGGCGGAGG AGACGGCGGCCTTCGAGGAGGTCATGAAGGACCTGAGCCTGACGCGGGCCGCGCGGCTCCAGCTCCAGAA ATCCCAGTACCTGCAACTGGGCCCCAGCCGAGGCCAGTACTATGGCGGGTCCCTGCCCAACGTGAACCAG ATCGGGAGTGGCACCATGGACCTGCCCTTCCAGCCCAGCGGATTTCTGGGGGAGGCCCTGGCAGCGGCTC CTGTCTCTCTGACCCCCTTCCAATCCTCGGGCCTGGACACCAGCCGGACCACCCGGCACCATGGGCTGGT GGACAGGGTGTACCGGGAGCGTGGCCGGCTCGGCTCCCCACACCGCCGGCCCCTGTCAGTGGACAAACAC GGACGGCAGGCCGACAGCTGCCCCTATGGCACCATGTACCTCTCACCACCCGCGGACACCAGCTGGAGAA GGACCAATTCTGACTCCGCCCTGCACCAGAGCACAATGACGCCCACGCAGCCAGAATCCTTTAGCAGTGG GTCCCAGGACGTGCACCAGAAAAGAGTCTTACTGTTAACAGTCCCAGGAATGGAAGAGACCACATCAGAG GCAGACAAAAACCTTTCCAAGCAAGCATGGGACACCAAGAAGACGGGGTCCAGGCCCAAGTCCTGTGAGG TCCCCGGAATCAACATCTTCCCGTCTGCCGACCAGGAAAACACTACAGCCCTGATCCCCGCCACCCACAA CACAGGGGGGTCCCTGCCCGACCTGACCAACATCCACTTCCCCTCCCCGCTCCCGACCCCGCTGGACCCC GAGGAGCCCACCTTCCCTGCACTGAGCAGCTCCAGCAGCACCGGCAACCTCGCGGCCAACCTGACGCACC TGGGCATCGGTGGCGCCGGCCAGGGAATGAGCACACCTGGCTCCTCTCCACAGCACCGCCCAGCTGGCGT CAGCCCCCTGTCCCTGAGCACAGAGGCAAGGCGTCAGCAGGCATCGCCCACCCTGTCCCCGCTGTCACCC ATCACTCAGGCTGTAGCCATGGACGCCCTGTCTCTGGAGCAGCAGCTGCCCTACGCCTTCTTCACCCAGG CGGGCTCCCAGCAGCCACCGCCGCAGCCCCAGCCCCCGCCGCCTCCTCCACCCGCGTCCCAGCAGCCACC ACCCCCGCCACCCCCACAGGCGCCCGTCCGCCTGCCCCCTGGTGGCCCCCTGTTGCCCAGCGCCAGCCTG ACTCGTGGGCCACAGCCGCCCCCGCTTGCAGTCACGGTACCGTCCTCTCTCCCCCAGTCCCCCCCAGAGA ACCCTGGCCAGCCATCGATGGGGATCGACATCGCCTCGGCGCCGGCTCTGCAGCAGTACCGCACTAGCGC CGGCTCCCCGGCCAACCAGTCTCCCACCTCGCCAGTCTCCAATCAAGGCTTCTCCCCAGGGAGCTCCCCG CAACACACTTCCACCCTGGGCAGCGTGTTTGGGGACGCGTACTATGAGCAGCAGATGGCGGCCAGGCAGG CCAATGCTCTGTCCCACCAGCTGGAGCAGTTCAACATGATGGAGAACGCCATCAGCTCCAGCAGCCTGTA CAGCCCGGGCTCCACACTCAACTACTCGCAGGCGGCCATGATGGGCCTCACGGGCAGCCACGGGAGCCTG CCGGACTCGCAGCAACTGGGATACGCCAGCCACAGTGGCATCCCCAACATCATCCTCACAGTGACAGGAG AGTCCCCCCCCAGCCTCTCTAAAGAACTGACCAGCTCTCTGGCCGGGGTCGGCGACGTCAGCTTCGACTC CGACAGCCAGTTTCCCCTGGACGAACTCAAGATCGACCCCCTGACCCTCGACGGACTGCACATGCTCAAC GACCCCGACATGGTTCTGGCCGACCCAGCCACCGAGGACACCTTCCGGATGGACCGCCTG AGCGGACCGACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA >RG224693 representing NM_001098482
Red=Cloning site Green=Tags(s) MATSNNPRKFSEKIALHNQKQAEETAAFEEVMKDLSLTRAARLQLQKSQYLQLGPSRGQYYGGSLPNVNQ IGSGTMDLPFQPSGFLGEALAAAPVSLTPFQSSGLDTSRTTRHHGLVDRVYRERGRLGSPHRRPLSVDKH GRQADSCPYGTMYLSPPADTSWRRTNSDSALHQSTMTPTQPESFSSGSQDVHQKRVLLLTVPGMEETTSE ADKNLSKQAWDTKKTGSRPKSCEVPGINIFPSADQENTTALIPATHNTGGSLPDLTNIHFPSPLPTPLDP EEPTFPALSSSSSTGNLAANLTHLGIGGAGQGMSTPGSSPQHRPAGVSPLSLSTEARRQQASPTLSPLSP ITQAVAMDALSLEQQLPYAFFTQAGSQQPPPQPQPPPPPPPASQQPPPPPPPQAPVRLPPGGPLLPSASL TRGPQPPPLAVTVPSSLPQSPPENPGQPSMGIDIASAPALQQYRTSAGSPANQSPTSPVSNQGFSPGSSP QHTSTLGSVFGDAYYEQQMAARQANALSHQLEQFNMMENAISSSSLYSPGSTLNYSQAAMMGLTGSHGSL PDSQQLGYASHSGIPNIILTVTGESPPSLSKELTSSLAGVGDVSFDSDSQFPLDELKIDPLTLDGLHMLN DPDMVLADPATEDTFRMDRL SGPTRTRRLE - GFP Tag - V |
| Restriction Sites |
SgfI-RsrII
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_001098482 |
| ORF Size | 1950 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_001098482.2 |
| RefSeq Size | 7009 bp |
| RefSeq ORF | 1953 bp |
| Locus ID | 23373 |
| UniProt ID | Q6UUV9 |
| Protein Families | Transcription Factors |
| Gene Summary | Transcriptional coactivator for CREB1 which activates transcription through both consensus and variant cAMP response element (CRE) sites. Acts as a coactivator, in the SIK/TORC signaling pathway, being active when dephosphorylated and acts independently of CREB1 'Ser-133' phosphorylation. Enhances the interaction of CREB1 with TAF4. Regulates the expression of specific CREB-activated genes such as the steroidogenic gene, StAR. Potent coactivator of PGC1alpha and inducer of mitochondrial biogenesis in muscle cells. In the hippocampus, involved in late-phase long-term potentiation (L-LTP) maintenance at the Schaffer collateral-CA1 synapses. May be required for dendritic growth of developing cortical neurons (By similarity). In concert with SIK1, regulates the light-induced entrainment of the circadian clock. In response to light stimulus, coactivates the CREB-mediated transcription of PER1 which plays an important role in the photic entrainment of the circadian clock.[UniProtKB/Swiss-Prot Function] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC224693 | CRTC1 (Myc-DDK-tagged)-Human CREB regulated transcription coactivator 1 (CRTC1), transcript variant 3 |
CNY 4,840.00 |
|
| RC224693L3 | Lenti ORF clone of Human CREB regulated transcription coactivator 1 (CRTC1), transcript variant 3, Myc-DDK-tagged |
CNY 7,600.00 |
|
| RC224693L4 | Lenti ORF clone of Human CREB regulated transcription coactivator 1 (CRTC1), transcript variant 3, mGFP tagged |
CNY 7,600.00 |
|
| SC316412 | CRTC1 (untagged)-Human CREB regulated transcription coactivator 1 (CRTC1), transcript variant 3 |
CNY 10,450.00 |
