SPG3A (ATL1) (NM_001127713) Human Tagged ORF Clone
CAT#: RG225949
- TrueORF®
ATL1 (tGFP-tagged) - Human atlastin GTPase 1 (ATL1), transcript variant 3
ORF Plasmid: DDK
"NM_001127713" in other vectors (4)
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CNY 5,230.00
Specifications
Product Data | |
Type | Human Tagged ORF Clone |
Tag | TurboGFP |
Synonyms | AD-FSP; atlastin1; FSP1; GBP3; HSN1D; SPG3; SPG3A |
Vector | pCMV6-AC-GFP |
E. coli Selection | Ampicillin (100 ug/mL) |
Mammalian Cell Selection | Neomycin |
Sequence Data |
>RG225949 representing NM_001127713
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGCCAAGAACCGCAGGGACAGAAACAGTTGGGGTGGATTTTCGGAAAAGACATATGAATGGAGCTCAG AAGAGGAGGAGCCAGTGAAAAAGGCAGGACCAGTCCAAGTCCTCATTGTCAAAGATGACCATTCCTTTGA GTTAGATGAAACTGCATTAAATCGGATCCTTCTCTCGGAGGCTGTCAGAGACAAGGAGGTTGTTGCTGTA TCTGTTGCTGGAGCATTTAGAAAAGGAAAATCATTCCTGATGGACTTCATGTTGAGATACATGTACAACC AGGAATCAGTTGATTGGGTTGGAGACTACAATGAACCATTGACTGGTTTTTCATGGAGAGGTGGATCTGA GCGAGAGACCACAGGAATTCAGATATGGAGTGAAATCTTCCTTATCAATAAACCTGATGGTAAAAAGGTT GCAGTGTTATTGATGGATACTCAGGGAACCTTTGATAGTCAGTCAACTTTGAGAGATTCAGCCACAGTAT TTGCCCTTAGCACAATGATCAGCTCAATACAGGTATATAACTTATCCCAAAATGTCCAGGAGGATGATCT TCAGCACCTCCAGCTTTTCACTGAGTATGGCAGACTGGCAATGGAGGAAACATTCCTGAAGCCATTTCAG AGTCTGATATTTCTTGTTCGAGACTGGAGTTTCCCATACGAATTTTCATATGGAGCCGATGGTGGTGCCA AATTCTTGGAAAAACGCCTCAAGGTCTCAGGGAACCAGCATGAAGAACTACAGAACGTCAGAAAACACAT CCATTCCTGTTTCACCAACATTTCCTGTTTTCTGCTACCTCATCCTGGCTTAAAAGTAGCTACCAATCCA AACTTTGATGGAAAATTGAAAGAAATAGATGATGAATTCATCAAAAACTTGAAAATACTGATTCCTTGGC TACTTAGTCCCGAGAGCCTAGATATTAAAGAGATCAATGGGAATAAAATCACCTGCCGGGGTCTGGTGGA GTACTTCAAGGCTTATATAAAGATCTATCAAGGTGAAGAATTACCACATCCCAAATCCATGTTACAGGCC ACAGCAGAAGCTAACAATTTAGCAGCCGTGGCAACTGCCAAGGACACATACAACAAAAAAATGGAAGAGA TTTGTGGTGGTGACAAACCATTTCTGGCCCCAAATGACTTGCAGACCAAACACCTGCAACTTAAGGAAGA ATCTGTGAAGCTATTCCGAGGGGTGAAGAAGATGGGTGGGGAAGAATTTAGCCGGCGTTACCTGCAGCAG TTGGAGAGTGAAATAGATGAACTTTACATCCAATATATCAAGCACAATGATAGCAAAAATATCTTCCATG CAGCTCGTACCCCAGCCACACTGTTTGTAGTCATCTTTATCACATATGTGATTGCTGGTGTGACTGGATT CATTGGTTTGGACATCATAGCTAGCCTATGCAATATGATAATGGGACTGACCCTTATCACCCTGTGCACT TGGGCATATATCCGGTACTCTGGAGAATACCGAGAGCTGGGAGCTGTAATAGACCAGGTGGCTGCAGCTC TGTGGGACCAGGCTTTGTACAAGCTTTACAGTGCAGCAGCAACCCACAGACATCTGTATCATCAAGCTTT CCCTACACCAAAGTCGGAATCTACTGAACAATCAGAAAAGAAAAAAATG ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA >RG225949 representing NM_001127713
Red=Cloning site Green=Tags(s) MAKNRRDRNSWGGFSEKTYEWSSEEEEPVKKAGPVQVLIVKDDHSFELDETALNRILLSEAVRDKEVVAV SVAGAFRKGKSFLMDFMLRYMYNQESVDWVGDYNEPLTGFSWRGGSERETTGIQIWSEIFLINKPDGKKV AVLLMDTQGTFDSQSTLRDSATVFALSTMISSIQVYNLSQNVQEDDLQHLQLFTEYGRLAMEETFLKPFQ SLIFLVRDWSFPYEFSYGADGGAKFLEKRLKVSGNQHEELQNVRKHIHSCFTNISCFLLPHPGLKVATNP NFDGKLKEIDDEFIKNLKILIPWLLSPESLDIKEINGNKITCRGLVEYFKAYIKIYQGEELPHPKSMLQA TAEANNLAAVATAKDTYNKKMEEICGGDKPFLAPNDLQTKHLQLKEESVKLFRGVKKMGGEEFSRRYLQQ LESEIDELYIQYIKHNDSKNIFHAARTPATLFVVIFITYVIAGVTGFIGLDIIASLCNMIMGLTLITLCT WAYIRYSGEYRELGAVIDQVAAALWDQALYKLYSAAATHRHLYHQAFPTPKSESTEQSEKKKM TRTRPLE - GFP Tag - V |
Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
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ACCN | NM_001127713 |
ORF Size | 1659 bp |
OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
Reference Data | |
RefSeq | NM_001127713.1, NP_001121185.1 |
RefSeq Size | 2821 bp |
RefSeq ORF | 1662 bp |
Locus ID | 51062 |
UniProt ID | Q8WXF7 |
Protein Families | Druggable Genome, Transmembrane |
Gene Summary | The protein encoded by this gene is a GTPase and a Golgi body transmembrane protein. The encoded protein can form a homotetramer and has been shown to interact with spastin and with mitogen-activated protein kinase kinase kinase kinase 4. This protein may be involved in axonal maintenance as evidenced by the fact that defects in this gene are a cause of spastic paraplegia type 3. Three transcript variants encoding two different isoforms have been found for this gene. [provided by RefSeq, Jul 2008] |
Documents
Product Manuals |
FAQs |
SDS |
Resources
Other Versions
SKU | Description | Size | Price |
---|---|---|---|
RC225949 | ATL1 (Myc-DDK-tagged)-Human atlastin GTPase 1 (ATL1), transcript variant 3 |
CNY 4,120.00 |
|
RC225949L3 | Lenti-ORF clone of ATL1 (Myc-DDK-tagged)-Human atlastin GTPase 1 (ATL1), transcript variant 3 |
CNY 6,650.00 |
|
RC225949L4 | Lenti-ORF clone of ATL1 (mGFP-tagged)-Human atlastin GTPase 1 (ATL1), transcript variant 3 |
CNY 6,650.00 |
|
SC323135 | ATL1 (untagged)-Human atlastin GTPase 1 (ATL1), transcript variant 3 |
CNY 8,840.00 |