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ILF3 (NM_017620) Human Tagged ORF Clone
CAT＃: RG226907
- TrueORF®
ILF3 (tGFP-tagged) - Human interleukin enhancer binding factor 3, 90kDa (ILF3), transcript variant 4
ORF Plasmid: DDK
"NM_017620" in other vectors (4)
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CNY 9,790.00
Product images
热销推荐Specifications
| Product Data | |
| Type | Human Tagged ORF Clone |
| Tag | TurboGFP |
| Synonyms | CBTF; DRBF; DRBP76; MMP4; MPHOSPH4; MPP4; MPP4110; NF-AT-90; NF90; NF90a; NF90b; NF90c; NF90ctv; NF110; NF110b; NFAR; NFAR-1; NFAR-2; NFAR2; NFAR90; NFAR110; TCP80; TCP110 |
| Vector | pCMV6-AC-GFP |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>RG226907 representing NM_017620
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGCGTCCAATGCGAATTTTTGTGAATGATGACCGCCATGTGATGGCAAAGCATTCTTCCGTTTATCCAA CACAAGAGGAGCTGGAGGCAGTCCAGAACATGGTGTCCCACACGGAGCGGGCGCTCAAAGCTGTGTCCGA CTGGATAGACGAGCAGGAAAAGGGTAGCAGCGAGCAGGCAGAGTCCGATAACATGGATGTGCCCCCAGAG GACGACAGTAAAGAAGGGGCTGGGGAACAGAAGACGGAGCACATGACCAGAACCCTGCGGGGAGTGATGC GGGTGGGCCTGGTGGCAAAGGGCCTCCTACTCAAGGGGGACTTGGATCTGGAGCTGGTGCTGCTGTGTAA GGAGAAGCCCACAACCGCCCTCCTGGACAAGGTGGCCGACAACCTGGCCATCCAGCTTGCTGCTGTAACA GAAGACAAGTACGAAATACTGCAATCTGTCGACGATGCTGCGATTGTGATAAAAAACACAAAAGAGCCTC CATTGTCCCTGACCATCCACCTGACATCCCCTGTTGTCAGAGAAGAAATGGAGAAAGTATTAGCTGGAGA AACGCTATCAGTCAACGACCCCCCGGACGTTCTGGACAGGCAGAAATGCCTTGCTGCCTTGGCGTCCCTC CGACACGCCAAGTGGTTCCAGGCCAGAGCCAACGGGCTGAAGTCTTGTGTCATTGTGATCCGGGTCTTGA GGGACCTGTGCACTCGCGTGCCCACCTGGGGTCCCCTCCGAGGCTGGCCTCTCGAGCTCCTGTGTGAGAA ATCCATTGGCACGGCCAACAGACCGATGGGTGCTGGCGAGGCCCTGCGGAGAGTGCTGGAGTGCCTGGCG TCGGGCATCGTGATGCCAGATGGTTCTGGCATTTATGACCCTTGTGAAAAAGAAGCCACTGATGCTATTG GGCATCTAGACAGACAGCAACGGGAAGATATCACACAGAGTGCGCAGCACGCACTGCGGCTCGCTGCCTT CGGCCAGCTCCATAAAGTCCTAGGCATGGACCCTCTGCCTTCCAAGATGCCCAAGAAACCAAAGAATGAA AACCCAGTGGACTACACCGTTCAGATCCCACCAAGCACCACCTATGCCATTACGCCCATGAAACGCCCAA TGGAGGAGGACGGGGAGGAGAAGTCGCCCAGCAAAAAGAAGAAGAAGATTCAGAAGAAAGAGGAGAAGGC AGAGCCCCCCCAGGCTATGAATGCCCTGATGCGGTTGAACCAGCTGAAGCCAGGGCTGCAGTACAAGCTG GTGTCCCAGACTGGGCCCGTCCATGCCCCCATCTTTACCATGTCTGTGGAGGTTGATGGCAATTCATTCG AGGCCTCTGGGCCCTCCAAAAAGACGGCCAAGCTGCACGTGGCCGTTAAGGTGTTACAGGACATGGGCTT GCCGACGGGTGCTGAAGGCAGGGACTCGAGCAAGGGGGAGGACTCGGCTGAGGAGACCGAGGCGAAGCCA GCAGTGGTGGCCCCTGCCCCAGTGGTAGAAGCTGTCTCCACCCCTAGTGCGGCCTTTCCCTCAGATGCCA CTGCCGAGAACGTAAAACAGCAGGGGCCGATCCTGACAAAGCACGGCAAGAACCCAGTCATGGAGCTGAA CGAGAAGAGGCGTGGGCTCAAGTACGAGCTCATCTCCGAGACCGGGGGCAGCCACGACAAGCGCTTCGTC ATGGAGGTCGAAGTGGATGGACAGAAGTTCCAAGGTGCTGGTTCCAACAAAAAGGTGGCGAAGGCCTACG CTGCTCTTGCTGCCCTAGAAAAGCTTTTCCCTGACACCCCTCTCGCCCTTGATGCCAACAAAAAGAAGAG AGCCCCAGTACCCGTCAGAGGGGGACCGAAATTTGCTGCTAAGCCACATAACCCTGGCTTCGGCATGGGA GGCCCCATGCACAACGAAGTGCCCCCACCCCCCAACCTTCGAGGGCGGGGAAGAGGCGGGAGCATCCGGG GACGAGGGCGCGGGCGAGGATTTGGTGGCGCCAACCATGGAGGCTACATGAATGCCGGTGCTGGGTATGG AAGCTATGGGTACGGAGGCAACTCGGCGACAGCAGGCTACAGTCAGTTCTACAGCAACGGAGGGCATTCT GGGAATGCCAGTGGCGGTGGCGGCGGGGGCGGTGGTGGCTCCTCCGGCTATGGCTCCTACTACCAAGGTG ACAACTACAACTCACCGGTGCCCCCAAAACACGCTGGGAAGAAGCAGCCGCACGGGGGCCAGCAGAAGCC CTCCTACGGCTCGGGCTACCAGTCCCACCAGGGCCAGCAGCAGTCCTACAACCAGAGCCCCTACAGCAAC TATGGCCCTCCACAGGGCAAGCAGAAAGGCTATAACCATGGACAAGGCAGCTACTCCTACTCGAACTCCT ACAACTCTCCCGGGGGCGGGGGCGGATCCGACTACAACTACGAGAGCAAATTCAACTACAGTGGTAGTGG AGGCCGAAGCGGCGGGAACAGCTACGGCTCAGGCGGGGCATCCTACAACCCAGGGTCACACGGGGGCTAC GGCGGAGGTTCTGGGGGCGGCTCCTCATACCAAGGCAAACAAGGAGGCTACTCACAGTCGAACTACAACT CCCCGGGGTCCGGCCAGAACTACAGTGGCCCTCCCAGCTCCTACCAGTCCTCACAAGGCGGCTATGGCAG AAACGCAGACCACAGCATGAACTACCAGTACAGA ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA >RG226907 representing NM_017620
Red=Cloning site Green=Tags(s) MRPMRIFVNDDRHVMAKHSSVYPTQEELEAVQNMVSHTERALKAVSDWIDEQEKGSSEQAESDNMDVPPE DDSKEGAGEQKTEHMTRTLRGVMRVGLVAKGLLLKGDLDLELVLLCKEKPTTALLDKVADNLAIQLAAVT EDKYEILQSVDDAAIVIKNTKEPPLSLTIHLTSPVVREEMEKVLAGETLSVNDPPDVLDRQKCLAALASL RHAKWFQARANGLKSCVIVIRVLRDLCTRVPTWGPLRGWPLELLCEKSIGTANRPMGAGEALRRVLECLA SGIVMPDGSGIYDPCEKEATDAIGHLDRQQREDITQSAQHALRLAAFGQLHKVLGMDPLPSKMPKKPKNE NPVDYTVQIPPSTTYAITPMKRPMEEDGEEKSPSKKKKKIQKKEEKAEPPQAMNALMRLNQLKPGLQYKL VSQTGPVHAPIFTMSVEVDGNSFEASGPSKKTAKLHVAVKVLQDMGLPTGAEGRDSSKGEDSAEETEAKP AVVAPAPVVEAVSTPSAAFPSDATAENVKQQGPILTKHGKNPVMELNEKRRGLKYELISETGGSHDKRFV MEVEVDGQKFQGAGSNKKVAKAYAALAALEKLFPDTPLALDANKKKRAPVPVRGGPKFAAKPHNPGFGMG GPMHNEVPPPPNLRGRGRGGSIRGRGRGRGFGGANHGGYMNAGAGYGSYGYGGNSATAGYSQFYSNGGHS GNASGGGGGGGGGSSGYGSYYQGDNYNSPVPPKHAGKKQPHGGQQKPSYGSGYQSHQGQQQSYNQSPYSN YGPPQGKQKGYNHGQGSYSYSNSYNSPGGGGGSDYNYESKFNYSGSGGRSGGNSYGSGGASYNPGSHGGY GGGSGGGSSYQGKQGGYSQSNYNSPGSGQNYSGPPSSYQSSQGGYGRNADHSMNYQYR TRTRPLE - GFP Tag - V |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_017620 |
| ORF Size | 2694 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_017620.2, NP_060090.2 |
| RefSeq Size | 6135 bp |
| RefSeq ORF | 2697 bp |
| Locus ID | 3609 |
| UniProt ID | Q12906 |
| Protein Families | Druggable Genome, Transcription Factors |
| Gene Summary | This gene encodes a double-stranded RNA (dsRNA) binding protein that complexes with other proteins, dsRNAs, small noncoding RNAs, and mRNAs to regulate gene expression and stabilize mRNAs. This protein (NF90, ILF3) forms a heterodimer with a 45 kDa transcription factor (NF45, ILF2) required for T-cell expression of interleukin 2. This complex has been shown to affect the redistribution of nuclear mRNA to the cytoplasm. Knockdown of NF45 or NF90 protein retards cell growth, possibly by inhibition of mRNA stabilization. In contrast, an isoform (NF110) of this gene that is predominantly restricted to the nucleus has only minor effects on cell growth when its levels are reduced. Alternative splicing results in multiple transcript variants encoding distinct isoforms.[provided by RefSeq, Dec 2014] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC226907 | ILF3 (Myc-DDK-tagged)-Human interleukin enhancer binding factor 3, 90kDa (ILF3), transcript variant 4 |
CNY 8,930.00 |
|
| RC226907L3 | Lenti-ORF clone of ILF3 (Myc-DDK-tagged)-Human interleukin enhancer binding factor 3, 90kDa (ILF3), transcript variant 4 |
CNY 10,830.00 |
|
| RC226907L4 | Lenti-ORF clone of ILF3 (mGFP-tagged)-Human interleukin enhancer binding factor 3, 90kDa (ILF3), transcript variant 4 |
CNY 10,830.00 |
|
| SC326279 | ILF3 (untagged)-Human interleukin enhancer binding factor 3, 90kDa (ILF3), transcript variant 4 |
CNY 17,290.00 |
