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POU5F1B (NM_001159542) Human Tagged ORF Clone
CAT＃: RG228288
- TrueORF®
POU5F1B (tGFP-tagged) - Human POU class 5 homeobox 1B (POU5F1B)
ORF Plasmid: DDK
"NM_001159542" in other vectors (4)
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CNY 5,256.00
Product images
Specifications
| Product Data | |
| Type | Human Tagged ORF Clone |
| Tag | TurboGFP |
| Synonyms | OCT4-PG1; OCT4PG1; OTF3C; OTF3P1; POU5F1P1; POU5F1P4; POU5FLC8; POU5FLC20 |
| Vector | pCMV6-AC-GFP |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>RG228288 representing NM_001159542
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGCGGGACACCTGGCTTCGGATTTCGCCTTCTCGCCCCCTCCAGGCGGTGGGGGTGATGGGCCATGGG GGGCGGAGCCGGGCTGGGTTGATCCTCTGACCTGGCTAAGCTTCCAAGGCCCTCCTGGAGGGCCAGGAAT CGGGCCGGGGGTTGGGCCAGGCTCTGAGGTGTGGGGGATTCCCCCTTGCCCCCCGCCGTATGAGTTATGT GGGGGGATGGCGTACTGTGGGCCTCAGGTTGGAGTGGGGCTAGTGCCCCAAGGCGGCTTGGAGACCTCTC AGCCTGAGAGCGAAGCAGGAGTCGGGGTGGAGAGCAACTCCAATGGGGCCTCCCCGGAACCCTGCACCGT CCCCCCTGGTGCCGTGAAGCTGGAGAAGGAGAAGCTAGAGCAAAACCCGGAGAAGTCCCAGGACATCAAA GCTCTGCAGAAAGAACTCGAGCAATTTGCCAAGCTCCTGAAGCAGAAGAGGATCACCCTGGGATATACAC AGGCCGATGTGGGGCTCATCCTGGGGGTTCTATTTGGGAAGGTGTTCAGCCAAAAGACCATCTGCCGCTT TGAGGCTCTGCAGCTTAGCTTCAAGAACATGTGTAAGCTGCGGCCCTTGCTGCAGAAGTGGGTGGAGGAA GCTGACAACAATGAAAATCTTCAGGAGATATGCAAAGCAGAAACCCTCATGCAGGCCCGAAAGAGAAAGC GAACCAGTATCGAGAACCGAGTGAGAGGCAACCTGGAGAATTTGTTCCTGCAGTGCCCGAAACCCACACT GCAGATCAGCCACATCGCCCAGCAGCTTGGGCTCGAGAAGGATGTGGTCCGAGTGTGGTTCTGTAACCGG CGCCAGAAGGGCAAGCGATCAAGCAGCGACTATGCACAACGAGAGGATTTTGAGGCTGCTGGGTCTCCTT TCTCAGGGGGACCAGTGTCCTTTCCTCCGGCCCCAGGGCCCCATTTTGGTACCCCAGGCTATGGGAGCCC TCACTTCACTGCACTGTACTCCTCAGTCCCTTTCCCTGAGGGGGAAGTCTTTCCCCCAGTCTCCGTCATC ACTCTGGGCTCTCCCATGCATTCAAAC ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA >RG228288 representing NM_001159542
Red=Cloning site Green=Tags(s) MAGHLASDFAFSPPPGGGGDGPWGAEPGWVDPLTWLSFQGPPGGPGIGPGVGPGSEVWGIPPCPPPYELC GGMAYCGPQVGVGLVPQGGLETSQPESEAGVGVESNSNGASPEPCTVPPGAVKLEKEKLEQNPEKSQDIK ALQKELEQFAKLLKQKRITLGYTQADVGLILGVLFGKVFSQKTICRFEALQLSFKNMCKLRPLLQKWVEE ADNNENLQEICKAETLMQARKRKRTSIENRVRGNLENLFLQCPKPTLQISHIAQQLGLEKDVVRVWFCNR RQKGKRSSSDYAQREDFEAAGSPFSGGPVSFPPAPGPHFGTPGYGSPHFTALYSSVPFPEGEVFPPVSVI TLGSPMHSN TRTRPLE - GFP Tag - V |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_001159542 |
| ORF Size | 1077 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_001159542.2 |
| RefSeq Size | 1599 bp |
| RefSeq ORF | 1080 bp |
| Locus ID | 5462 |
| UniProt ID | Q06416 |
| Gene Summary | This intronless gene was thought to be a transcribed pseudogene of POU class 5 homeobox 1, however, it has been reported that this gene can encode a functional protein. The encoded protein is nearly the same length as and highly similar to the POU class 5 homeobox 1 transcription factor, has been shown to be a weak transcriptional activator and may play a role in carcinogenesis and eye development. [provided by RefSeq, Apr 2009] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC228288 | POU5F1B (Myc-DDK-tagged)-Human POU class 5 homeobox 1B (POU5F1B) |
CNY 3,656.00 |
|
| RC228288L3 | Lenti ORF clone of Human POU class 5 homeobox 1B (POU5F1B), Myc-DDK-tagged |
CNY 5,890.00 |
|
| RC228288L4 | Lenti ORF clone of Human POU class 5 homeobox 1B (POU5F1B), mGFP tagged |
CNY 5,890.00 |
|
| SC326923 | POU5F1B (untagged)-Human POU class 5 homeobox 1B (POU5F1B) |
CNY 3,656.00 |
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