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SLC12A4 (NM_001145963) Human Tagged ORF Clone
CAT＃: RG228637
- TrueORF®
SLC12A4 (tGFP-tagged) - Human solute carrier family 12 (potassium/chloride transporters), member 4 (SLC12A4), transcript variant 4
ORF Plasmid: DDK
"NM_001145963" in other vectors (4)
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CNY 11,780.00
Product images
CNY 1,000.00
Specifications
| Product Data | |
| Type | Human Tagged ORF Clone |
| Tag | TurboGFP |
| Synonyms | CTC-479C5.17; hKCC1; KCC1 |
| Vector | pCMV6-AC-GFP |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>RG228637 representing NM_001145963
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGCAGCCGAAGGTGCTGTGTGCGGCTTCGTCTATCTGGAGGGCACTGCCTGGGCGGTCCCTGAGGACA CCGAACCCTTGGCATCCTGCACATTGGGACATGGCAACCACAGAGAGAGCAGCCCTTTTCTTTCCCCCTT GGAGGCTTCCAGAGGAATTGACTACTATGACAGGAACCTGGCACTGTTTGAGGAAGAGCTGGACATCCGC CCAAAGGTATCGTCTCTTCTGGGAAAGCTCGTCAGCTACACCAACCTCACCCAGGGCGCCAAAGAGCATG AGGAGGCCGAGAGTGGGGAGGGCACCCGCCGGAGGGCAGCCGAGGCACCCAGCATGGGCACCCTCATGGG GGTGTACCTGCCCTGCCTGCAGAATATCTTTGGGGTTATCCTCTTCCTGCGGCTGACCTGGATGGTGGGC ACAGCAGGTGTGCTACAGGCCCTCCTCATCGTGCTTATCTGCTGCTGTTGTACCCTGCTGACGGCCATCT CCATGAGTGCCATCGCCACCAACGGTGTGGTTCCAGCTGGGGGCTCCTATTTCATGATCTCTCGTTCACT GGGGCCAGAATTTGGAGGTGCTGTGGGCCTGTGCTTCTACCTGGGAACAACATTCGCAGCAGCCATGTAC ATCCTGGGGGCCATCGAGATCTTGCTGACCTACATTGCCCCACCAGCTGCCATTTTTTACCCATCGGGTG CTCATGACACGTCGAATGCCACTTTGAACAATATGCGTGTGTATGGGACCATTTTCCTGACCTTCATGAC CCTGGTGGTGTTTGTGGGGGTCAAGTATGTGAACAAATTTGCCTCGCTCTTCCTGGCCTGTGTGATCATC TCCATCCTCTCCATCTATGCTGGGGGCATAAAGTCTATATTTGACCCTCCCGTGTTTCCGGTATGCATGC TGGGCAACAGGACCCTGTCCCGGGACCAGTTTGACATCTGTGCCAAGACAGCTGTAGTGGACAATGAGAC AGTGGCCACCCAGCTATGGAGTTTCTTCTGCCACAGCCCCAACCTTACGACCGACTCCTGTGACCCCTAC TTCATGCTCAACAATGTGACCGAGATCCCTGGCATCCCCGGGGCAGCTGCTGGTGTGCTCCAGGAAAACC TGTGGAGCGCCTACCTGGAGAAGGGTGACATCGTGGAGAAGCATGGGCTGCCCTCCGCAGATGCCCCGAG CCTGAAGGAGAGCCTGCCTCTGTACGTGGTCGCTGACATCGCCACATCCTTCACCGTGCTGGTCGGCATC TTCTTCCCTTCTGTAACAGGCATCATGGCTGGCTCAAACCGCTCTGGGGACCTTCGTGACGCCCAGAAGT CTATCCCTGTGGGGACCATTCTGGCCATCATTACAACTTCCCTCGTGTACTTCAGCAGTGTGGTTCTCTT TGGTGCCTGCATTGAGGGTGTGGTTCTCCGGGACAAGTATGGCGATGGTGTCAGCAGGAACTTGGTGGTG GGCACACTGGCCTGGCCTTCACCCTGGGTCATCGTCATCGGCTCCTTCTTTTCAACGTGTGGCGCTGGCC TCCAGAGCCTCACAGGGGCACCACGCCTATTGCAGGCCATTGCCAAGGACAACATCATCCCCTTCCTCCG GGTGTTTGGCCACGGGAAGGTGAATGGTGAACCCACATGGGCACTCCTCCTGACGGCACTCATCGCCGAG CTGGGCATCCTCATCGCCTCCCTCGACATGGTGGCCCCCATCTTATCCATGTTCTTTCTGATGTGCTACC TGTTCGTGAACCTCGCCTGTGCGGTGCAGACACTCCTGAGGACCCCCAACTGGCGGCCCCGGTTCAAGTA CTATCACTGGGCGCTGTCCTTCCTGGGCATGAGTCTCTGCCTGGCCCTTATGTTTGTCTCCTCCTGGTAC TATGCCCTGGTGGCCATGCTCATCGCCGGCATGATCTACAAATACATCGAGTACCAAGGGGCTGAGAAGG AGTGGGGTGACGGGATCCGAGGCCTGTCCCTGAGCGCTGCCCGCTACGCGCTGTTGCGGCTGGAGGAGGG GCCTCCTCACACCAAGAACTGGCGGCCGCAGCTGCTGGTGCTGCTGAAGCTGGACGAGGACCTCCACGTG AAGTACCCGCGGCTCCTCACCTTCGCCTCCCAGCTCAAGGCTGGCAAGGGCCTGACCATTGTTGGTTCTG TCATCCAGGGGAGCTTCTTGGAGAGCTATGGCGAGGCTCAGGCCGCCGAGCAGACCATCAAGAACATGAT GGAAATTGAGAAGGTGAAGGGCTTCTGCCAGGTGGTGGTGGCCAGCAAGGTGCGGGAGGGGCTGGCCCAC CTCATCCAGTCCTGTGGCCTGGGAGGCATGCGGCATAACTCCGTGGTGCTGGGCTGGCCCTACGGCTGGC GACAGAGCGAGGACCCCCGTGCCTGGAAGACCTTCATTGACACCGTGCGCTGCACTACGGCTGCCCACCT GGCCCTGCTCGTGCCCAAGAACATCGCCTTCTACCCCAGCAACCACGAGCGCTACCTGGAGGGCCACATA GACGTGTGGTGGATCGTGCACGATGGTGGCATGCTCATGCTTCTGCCCTTCCTGCTGCGCCAGCATAAGG TCTGGAGGAAGTGCCGGATGCGCATCTTCACAGTGGCCCAGATGGATGACAACAGCATCCAGATGAAGAA GGACCTGGCTGTCTTTCTGTACCATCTGCGCCTTGAGGCCGAGGTGGAGGTGGTGGAGATGCATAACAGT GACATCTCTGCATACACCTACGAGCGGACGCTGATGATGGAGCAGCGGTCGCAGATGCTGCGGCAGATGA GACTGACCAAGACTGAGCGGGAGCGAGAAGCCCAGCTGGTCAAGGATCGGCACTCGGCCCTGCGGCTGGA GAGCCTGTACTCGGACGAGGAAGATGAGTCTGCAGTGGGGGCTGACAAGATCCAGATGACGTGGACCAGG GACAAGTACATGACTGAGACCTGGGACCCCAGCCATGCCCCTGACAATTTCCGGGAGCTGGTGCACATTA AGCCGGACCAATCCAATGTGCGGCGCATGCACACTGCTGTGAAGCTCAATGAAGTCATTGTCACGCGCTC CCACGACGCCCGCCTGGTTCTCCTAAACATGCCTGGCCCACCCAGGAACAGTGAGGGCGACGAGAACTAC ATGGAGTTCCTCGAGGTGCTGACCGAGGGCCTTGAGCGGGTGCTGTTGGTGCGCGGTGGTGGCCGTGAAG TCATCACCATCTACTCC ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA >RG228637 representing NM_001145963
Red=Cloning site Green=Tags(s) MAAEGAVCGFVYLEGTAWAVPEDTEPLASCTLGHGNHRESSPFLSPLEASRGIDYYDRNLALFEEELDIR PKVSSLLGKLVSYTNLTQGAKEHEEAESGEGTRRRAAEAPSMGTLMGVYLPCLQNIFGVILFLRLTWMVG TAGVLQALLIVLICCCCTLLTAISMSAIATNGVVPAGGSYFMISRSLGPEFGGAVGLCFYLGTTFAAAMY ILGAIEILLTYIAPPAAIFYPSGAHDTSNATLNNMRVYGTIFLTFMTLVVFVGVKYVNKFASLFLACVII SILSIYAGGIKSIFDPPVFPVCMLGNRTLSRDQFDICAKTAVVDNETVATQLWSFFCHSPNLTTDSCDPY FMLNNVTEIPGIPGAAAGVLQENLWSAYLEKGDIVEKHGLPSADAPSLKESLPLYVVADIATSFTVLVGI FFPSVTGIMAGSNRSGDLRDAQKSIPVGTILAIITTSLVYFSSVVLFGACIEGVVLRDKYGDGVSRNLVV GTLAWPSPWVIVIGSFFSTCGAGLQSLTGAPRLLQAIAKDNIIPFLRVFGHGKVNGEPTWALLLTALIAE LGILIASLDMVAPILSMFFLMCYLFVNLACAVQTLLRTPNWRPRFKYYHWALSFLGMSLCLALMFVSSWY YALVAMLIAGMIYKYIEYQGAEKEWGDGIRGLSLSAARYALLRLEEGPPHTKNWRPQLLVLLKLDEDLHV KYPRLLTFASQLKAGKGLTIVGSVIQGSFLESYGEAQAAEQTIKNMMEIEKVKGFCQVVVASKVREGLAH LIQSCGLGGMRHNSVVLGWPYGWRQSEDPRAWKTFIDTVRCTTAAHLALLVPKNIAFYPSNHERYLEGHI DVWWIVHDGGMLMLLPFLLRQHKVWRKCRMRIFTVAQMDDNSIQMKKDLAVFLYHLRLEAEVEVVEMHNS DISAYTYERTLMMEQRSQMLRQMRLTKTEREREAQLVKDRHSALRLESLYSDEEDESAVGADKIQMTWTR DKYMTETWDPSHAPDNFRELVHIKPDQSNVRRMHTAVKLNEVIVTRSHDARLVLLNMPGPPRNSEGDENY MEFLEVLTEGLERVLLVRGGGREVITIYS TRTRPLE - GFP Tag - V |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_001145963 |
| ORF Size | 3237 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq | NM_001145963.2 |
| RefSeq Size | 4652 bp |
| RefSeq ORF | 3240 bp |
| Locus ID | 6560 |
| UniProt ID | Q9UP95 |
| Protein Families | Transmembrane |
| Gene Summary | This gene encodes a member of the SLC12A transporter family. The encoded protein mediates the coupled movement of potassium and chloride ions across the plasma membrane. This gene is expressed ubiquitously. Multiple alternatively spliced transcript variants encoding distinct isoforms have been found for this gene. [provided by RefSeq, Jan 2013] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC228637 | SLC12A4 (Myc-DDK-tagged)-Human solute carrier family 12 (potassium/chloride transporters), member 4 (SLC12A4), transcript variant 4 |
CNY 10,740.00 |
|
| RC228637L3 | Lenti-ORF clone of SLC12A4 (Myc-DDK-tagged)-Human solute carrier family 12 (potassium/chloride transporters), member 4 (SLC12A4), transcript variant 4 |
CNY 12,640.00 |
|
| RC228637L4 | Lenti-ORF clone of SLC12A4 (mGFP-tagged)-Human solute carrier family 12 (potassium/chloride transporters), member 4 (SLC12A4), transcript variant 4 |
CNY 12,640.00 |
|
| SC327272 | SLC12A4 (untagged)-Human solute carrier family 12 (potassium/chloride transporters) member 4 (SLC12A4) transcript variant 4 |
CNY 20,710.00 |
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