United States
Germany
Japan
United Kingdom
China
EIF4ENIF1 (NM_001164502) Human Tagged ORF Clone
CAT＃: RG229015
- TrueORF®
EIF4ENIF1 (tGFP-tagged) - Human eukaryotic translation initiation factor 4E nuclear import factor 1 (EIF4ENIF1), transcript variant 3
ORF Plasmid: DDK
"NM_001164502" in other vectors (4)
Need custom modification / cloning service?
Get a free quote
CNY 6,370.00
Product images
Specifications
| Product Data | |
| Type | Human Tagged ORF Clone |
| Tag | TurboGFP |
| Synonyms | 4E-T; Clast4 |
| Vector | pCMV6-AC-GFP |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>RG229015 representing NM_001164502
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGATAGGAGAAGTATGGGTGAAACAGAAAGTGGAGATGCTTTCCTTGACCTGAAGAAGCCTCCTGCCT CCAAATGCCCCCATCGCTATACAAAAGAAGAACTCTTGGATATAAAAGAACTCCCCCATTCCAAACAGAG GCCTTCATGCCTTTCTGAAAAATATGACAGTGATGGTGTCTGGGACCCTGAGAAGTGGCATGCCTCTCTC TACCCAGCTTCAGGGCGGAGCTCACCAGTGGAAAGTCTGAAGAAAGAGTTGGATACAGACCGGCCTTCCC TGGTGCGCAGGATAGTAGGTATAGTAGAGTGCAATGGAGGAGTGGCCGAAGAGGATGAAGTGGAGGTCAT CCTTGCACAGGAGCCTGCGGCTGATCAGGAAGTGCCAAGGGATGCTGTCTTGCCTGAGCAGTCCCCAGGA GACTTTGACTTTAATGAGTTCTTTAACCTTGATAAGGTGCCATGCTTGGCTTCGATGATAGAAGATGTTT TGGGAGAAGGGTCAGTCTCTGCCAGTCGGTTCAGTAGGTGGTTCTCTAACCCGAGCAGATCAGGAAGCCG ATCCAGCAGTCTTGGGTCAACACCACATGAAGAGCTAGAGAGACTTGCAGGTCTGGAGCAAGCCATCCTC TCTCCTGGACAGAACTCGGGGAATTACTTTGCTCCTATACCATTGGAAGACCATGCTGAAAATAAAGTGG ATATTTTAGAAATGCTACAGAAAGCCAAAGTGGATTTGAAACCTCTTCTTTCCAGCCTTTCTGCAAATAA AGAAAAACTTAAAGAAAGCTCACATTCAGGGGTTGTGCTTTCAGTGGAGGAGGTAGAAGCAGGTCTGAAG GGCTTGAAGGTTGACCAGCAAGTGAAGAATTCAACTCCCTTCATGGCAGAACACCTAGAAGAGACCTTGA GTGCCGTAACCAACAATCGACAACTGAAGAAAGACGGAGACATGACTGCGTTCAACAAGCTAGTGAGCAC AATGAAGCGAAACCTTGAAAGCCATTTGATGTCCCCTGCTGAGATTCCAGGCCAGCCTGTCCCTAAGAAC ATCCTGCAGGAACTTCTGGGTCAACCAGTTCAGAGACCTGCTTCTTCCAATCTTCTGAGTGGCCTTATGG GGAGCTTGGAGCCTACAACATCTTTACTGGGCCAAAGAGCACCCTCTCCTCCCTTGTCACAGGTGTTTCA AACTCGAGCAGCCTCAGCTGACTACCTTCGCCCAAGAATACCATCACCAATTGGTTTCACACCAGGACCA CAGCAGCTACTCGGAGATCCATTCCAAGGCATGCGCAAACCCATGAGCCCCATCACAGCCCAGCAGATGA GCCAGCTGGAGTTGCAACAGGCAGCTTTAGAAGGGCTGGCCTTGCCACATGACCTTGCTGTACAGGCAGC AAACTTCTACCAGCCTGGTTTTGGCAAACCACAGGTGGACAGAACCAGAGATGGATTCAGAAACAGGCAA CAGCGAGTGACCAAGTCACCAGCACCCGTGCATCGAGGGAATTCCTCTTCCCCTGCCCCTGCTGCCTCCA TCACAAGCATGCTTTCTCCTTCCTTTACCCCTACCTCAGTGATTCGTAAGATGTACGAGAGCAAAGAGAA AAGCAAGGAGGAGCCAGCATCTGGAAAAGCAGCTCTTGGTGACAGTAAAGAGGATACTCAGAAGGCCAGT GAAGAAAACCTCCTGTCATCCAGTTCTGTACCCAGTGCCGATCGAGACTCTTCTCCCACTACAAATTCCA AACTGTCAGCATTACAGAGGTCTTCGTGTTCCACCCCACTGTCCCAGGCCAACCGTTACACCAAAGAACA AGATTATCGACCTAAAGCAACTGGGAGAAAAACACCCACCTTGGCATCCCCAGTTCCTACAACACCTTTT CTCCGCCCTGTCCACCAAGTTCCCCTTGTCCCCCATGTCCCTATGGTTAGGCCTGCTCACCAGCTTCACC CAGGGTTGGTACAGAGGATGCTGGCCCAGGGAGTACATCCACAGCATCTTCCAAGTTTGCTCCAAACTGG TGTGCTTCCTCCTGGGATGGACTTGAGTCATTTACAGGGAATATCTGGCCCCATCCTGGGTCAGCCCTTT TACCCTTTACCTGCTGCTAGTCACCCTCTCTTAAACCCTCGTCCTGGAACACCTCTGCATCTGGCAATGG TGCAACAGCAGCTACAGCGCTCAGTTCTGCATCCTCCAGGCTCTGGTTCCCATGCAGCAGCTGTCAGCGT TCAGACAACCCCTCAGAACGTGCCCAGCCGGTCAGGCCTGCCCCACATGCACTCCCAGCTGGAGCATCGC CCCAGCCAGAGGAGCAGCTCCCCTGTGGGCCTTGCCAAATGGTTTGGCTCAGATGTGCTACAGCAACCCC TGCCCTCCATGCCCGCCAAAGTTATCAGTGTAGATGAATTGGAATACCGACAG ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA >RG229015 representing NM_001164502
Red=Cloning site Green=Tags(s) MDRRSMGETESGDAFLDLKKPPASKCPHRYTKEELLDIKELPHSKQRPSCLSEKYDSDGVWDPEKWHASL YPASGRSSPVESLKKELDTDRPSLVRRIVGIVECNGGVAEEDEVEVILAQEPAADQEVPRDAVLPEQSPG DFDFNEFFNLDKVPCLASMIEDVLGEGSVSASRFSRWFSNPSRSGSRSSSLGSTPHEELERLAGLEQAIL SPGQNSGNYFAPIPLEDHAENKVDILEMLQKAKVDLKPLLSSLSANKEKLKESSHSGVVLSVEEVEAGLK GLKVDQQVKNSTPFMAEHLEETLSAVTNNRQLKKDGDMTAFNKLVSTMKRNLESHLMSPAEIPGQPVPKN ILQELLGQPVQRPASSNLLSGLMGSLEPTTSLLGQRAPSPPLSQVFQTRAASADYLRPRIPSPIGFTPGP QQLLGDPFQGMRKPMSPITAQQMSQLELQQAALEGLALPHDLAVQAANFYQPGFGKPQVDRTRDGFRNRQ QRVTKSPAPVHRGNSSSPAPAASITSMLSPSFTPTSVIRKMYESKEKSKEEPASGKAALGDSKEDTQKAS EENLLSSSSVPSADRDSSPTTNSKLSALQRSSCSTPLSQANRYTKEQDYRPKATGRKTPTLASPVPTTPF LRPVHQVPLVPHVPMVRPAHQLHPGLVQRMLAQGVHPQHLPSLLQTGVLPPGMDLSHLQGISGPILGQPF YPLPAASHPLLNPRPGTPLHLAMVQQQLQRSVLHPPGSGSHAAAVSVQTTPQNVPSRSGLPHMHSQLEHR PSQRSSSPVGLAKWFGSDVLQQPLPSMPAKVISVDELEYRQ TRTRPLE - GFP Tag - V |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_001164502 |
| ORF Size | 2433 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_001164502.1, NP_001157974.1 |
| RefSeq Size | 3182 bp |
| RefSeq ORF | 2436 bp |
| Locus ID | 56478 |
| UniProt ID | Q9NRA8 |
| Gene Summary | The protein encoded by this gene is a nucleocytoplasmic shuttle protein for the translation initiation factor eIF4E. This shuttle protein interacts with the importin alpha-beta complex to mediate nuclear import of eIF4E. It is predominantly cytoplasmic; its own nuclear import is regulated by a nuclear localization signal and nuclear export signals. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Aug 2009] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC229015 | EIF4ENIF1 (Myc-DDK-tagged)-Human eukaryotic translation initiation factor 4E nuclear import factor 1 (EIF4ENIF1), transcript variant 3 |
CNY 5,800.00 |
|
| RC229015L3 | Lenti-ORF clone of EIF4ENIF1 (Myc-DDK-tagged)-Human eukaryotic translation initiation factor 4E nuclear import factor 1 (EIF4ENIF1), transcript variant 3 |
CNY 7,700.00 |
|
| RC229015L4 | Lenti-ORF clone of EIF4ENIF1 (mGFP-tagged)-Human eukaryotic translation initiation factor 4E nuclear import factor 1 (EIF4ENIF1), transcript variant 3 |
CNY 7,700.00 |
|
| SC327650 | EIF4ENIF1 (untagged)-Human eukaryotic translation initiation factor 4E nuclear import factor 1 (EIF4ENIF1) transcript variant 3 |
CNY 7,220.00 |
其它EIF4ENIF1产品
