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ARHGAP25 (NM_001007231) Human Tagged ORF Clone
CAT＃: RG229321
- TrueORF®
ARHGAP25 (tGFP-tagged) - Human Rho GTPase activating protein 25 (ARHGAP25), transcript variant 1
ORF Plasmid: DDK
"NM_001007231" in other vectors (5)
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CNY 7,220.00
Product images
CNY 1,999.00
CNY 2,700.00
Specifications
| Product Data | |
| Type | Human Tagged ORF Clone |
| Tag | TurboGFP |
| Synonyms | HEL-S-308; KAIA0053 |
| Vector | pCMV6-AC-GFP |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>RG229321 representing NM_001007231
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGTCCCTAAAATTGCCAAGGAACTGGGATTTCAACCTGAAAGTGGAGGCTGCGAAAATAGCTCGGTCAA GGAGTGTGATGACTGGCGAGCAGATGGCTGCCTTCCATCCATCGTCCACCCCCAACCCGCTGGAGAGGCC CATCAAGATGGGCTGGCTGAAGAAGCAGAGGTCCATCGTGAAGAACTGGCAGCAGAGGTACTTTGTGCTG AGGGCGCAGCAGCTCTACTACTACAAGGATGAAGAGGACACGAAGCCCCAGGGCTGCATGTATCTACCAG GATGTACAATCAAGGAGATCGCCACAAACCCAGAAGAAGCTGGGAAGTTTGTCTTTGAAATCATTCCAGC CTCATGGGACCAGAATCGCATGGGACAGGACTCCTATGTCCTCATGGCCAGCTCTCAGGCGGAGATGGAG GAGTGGGTTAAATTCCTCAGGAGAGTTGCTGGCACACCCTGTGGAGCAGTGTTTGGCCAGCGCTTGGATG AGACTGTGGCCTATGAACAGAAATTCGGCCCCCATCTGGTGCCCATCCTGGTGGAGAAATGTGCAGAGTT CATCCTGGAGCACGGCCGGAATGAAGAGGGCATCTTCCGTCTGCCTGGGCAGGACAACCTGGTGAAGCAG CTGAGAGACGCTTTTGATGCTGGGGAGCGGCCCTCCTTTGACAGAGACACAGATGTGCACACTGTGGCTT CCCTGTTAAAGCTCTACCTCCGAGACCTCCCAGAGCCCGTGGTTCCCTGGAGCCAGTACGAAGGGTTCCT GCTCTGTGGGCAGCTCACGAATGCGGATGAGGCAAAGGCTCAGCAGGAGTTGATGAAGCAGCTCTCCATC CTTCCTCGTGACAACTATAGTCTCCTGAGCTACATCTGCAGGTTCCTACATGAAATACAGCTGAACTGTG CTGTTAACAAGATGAGTGTGGACAACCTGGCTACTGTGATTGGTGTGAATCTCATCAGGTCGAAGGTCGA AGACCCTGCCGTGATCATGAGAGGGACTCCTCAGATCCAAAGAGTGATGACTATGATGATCAGAGACCAT GAAGTCCTCTTCCCCAAGTCCAAGGATATACCCCTGTCACCCCCTGCCCAGAAAAATGACCCCAAGAAAG CTCCAGTGGCCCGAAGCTCTGTAGGCTGGGATGCCACTGAAGACCTCCGAATTTCTAGGACAGACAGCTT CAGTAGCATGACAAGCGACTCTGATACAACCAGCCCCACCGGACAGCAGCCGAGCGATGCGTTTCCGGAG GACAGCAGCAAAGTACCCAGGGAAAAGCCAGGAGACTGGAAAATGCAATCTCGTAAAAGGACTCAAACAC TCCCTAACCGGAAATGTTTCTTGACATCAGCTTTTCAGGGTGCCAACAGCAGCAAAATGGAGATCTTTAA AAATGAATTCTGGTCGCCTTCCTCAGAGGCTAAGGCAGGGGAAGGGCACAGGAGAACGATGTCTCAAGAC TTGCGCCAACTTTCTGACTCCCAACGGACTTCCACCTACGATAACGTCCCTTCCCTGCCAGGGTCCCCTG GGGAGGAAGCCAGTGCACTCTCTTCCCAAGCCTGTGACTCCAAGGGAGATACTCTTGCCAGTCCAAACTC TGAAACTGGGCCTGGAAAAAAGAACTCTGGAGAAGAGGAAATTGATTCTTTGCAGAGGATGGTCCAAGAG CTACGAAAGGAAATAGAAACACAGAAGCAAATGTATGAGGAACAGATTAAAAACCTTGAGAAGGAAAATT ATGACGTTTGGGCTAAAGTGGTGAGGCTCAATGAAGAACTGGAGAAGGAAAAGAAGAAGTCTGCAGCCCT AGAGATCAGCCTCCGCAACATGGAGCGCTCCCGGGAGGATGTTGAGAAGAGGAACAAGGCCTTGGAAGAA GAAGTCAAGGAATTTGTCAAATCCATGAAGGAACCCAAGACCGAGGCT ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA >RG229321 representing NM_001007231
Red=Cloning site Green=Tags(s) MSLKLPRNWDFNLKVEAAKIARSRSVMTGEQMAAFHPSSTPNPLERPIKMGWLKKQRSIVKNWQQRYFVL RAQQLYYYKDEEDTKPQGCMYLPGCTIKEIATNPEEAGKFVFEIIPASWDQNRMGQDSYVLMASSQAEME EWVKFLRRVAGTPCGAVFGQRLDETVAYEQKFGPHLVPILVEKCAEFILEHGRNEEGIFRLPGQDNLVKQ LRDAFDAGERPSFDRDTDVHTVASLLKLYLRDLPEPVVPWSQYEGFLLCGQLTNADEAKAQQELMKQLSI LPRDNYSLLSYICRFLHEIQLNCAVNKMSVDNLATVIGVNLIRSKVEDPAVIMRGTPQIQRVMTMMIRDH EVLFPKSKDIPLSPPAQKNDPKKAPVARSSVGWDATEDLRISRTDSFSSMTSDSDTTSPTGQQPSDAFPE DSSKVPREKPGDWKMQSRKRTQTLPNRKCFLTSAFQGANSSKMEIFKNEFWSPSSEAKAGEGHRRTMSQD LRQLSDSQRTSTYDNVPSLPGSPGEEASALSSQACDSKGDTLASPNSETGPGKKNSGEEEIDSLQRMVQE LRKEIETQKQMYEEQIKNLEKENYDVWAKVVRLNEELEKEKKKSAALEISLRNMERSREDVEKRNKALEE EVKEFVKSMKEPKTEA TRTRPLE - GFP Tag - V |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_001007231 |
| ORF Size | 1938 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_001007231.3 |
| RefSeq Size | 3020 bp |
| RefSeq ORF | 1941 bp |
| Locus ID | 9938 |
| UniProt ID | P42331 |
| Gene Summary | ARHGAPs, such as ARHGAP25, encode negative regulators of Rho GTPases (see ARHA; MIM 165390), which are implicated in actin remodeling, cell polarity, and cell migration (Katoh and Katoh, 2004 [PubMed 15254788]).[supplied by OMIM, Mar 2008] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC229321 | ARHGAP25 (Myc-DDK-tagged)-Human Rho GTPase activating protein 25 (ARHGAP25), transcript variant 1 |
CNY 4,808.00 |
|
| RC229321L3 | Lenti-ORF clone of ARHGAP25 (Myc-DDK-tagged)-Human Rho GTPase activating protein 25 (ARHGAP25), transcript variant 1 |
CNY 8,460.00 |
|
| RC229321L4 | Lenti-ORF clone of ARHGAP25 (mGFP-tagged)-Human Rho GTPase activating protein 25 (ARHGAP25), transcript variant 1 |
CNY 8,460.00 |
|
| SC301173 | ARHGAP25 (untagged)-Human Rho GTPase activating protein 25 (ARHGAP25), transcript variant 1 |
CNY 10,360.00 |
|
| SC327956 | ARHGAP25 (untagged)-Human Rho GTPase activating protein 25 (ARHGAP25) transcript variant 1 |
CNY 10,360.00 |
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