MEPE (NM_001184695) Human Tagged ORF Clone
CAT#: RG230031
- TrueORF®
MEPE (tGFP-tagged) - Human matrix extracellular phosphoglycoprotein (MEPE), transcript variant 3
ORF Plasmid: DDK
"NM_001184695" in other vectors (4)
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CNY 4,370.00
Specifications
Product Data | |
Type | Human Tagged ORF Clone |
Tag | TurboGFP |
Synonyms | OF45 |
Vector | pCMV6-AC-GFP |
E. coli Selection | Ampicillin (100 ug/mL) |
Mammalian Cell Selection | Neomycin |
Sequence Data |
>RG230031 representing NM_001184695
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGTCAATTTATCCTAAGTCAACTGGGAATAAAGGGTTTGAGGATGGAGATGATGCTATCAGCAAACTAC ATGACCAAGAAGAATATGGCGCAGCTCTCATCAGAAATAACATGCAACATATAATGGGGCCAGTGACTGC GATTAAACTCCTGGGGGAAGAAAACAAAGAGAACACACCTAGGAATGTTCTAAACATAATCCCAGCAAGT ATGAATTATGCTAAAGCACACTCGAAGGATAAAAAGAAGCCTCAAAGAGATTCCCAAGCCCAGAAAAGTC CAGTAAAAAGCAAAAGCACCCATCGTATTCAACACAACATTGACTACCTAAAACATCTCTCAAAAGTCAA AAAAATCCCCAGTGATTTTGAAGGCAGCGGTTATACAGATCTTCAAGAGAGAGGGGACAATGATATATCT CCTTTCAGTGGGGACGGCCAACCTTTTAAGGACATTCCTGGTAAAGGAGAAGCTACTGGTCCTGACCTAG AAGGCAAAGATATTCAAACAGGGTTTGCAGGCCCAAGTGAAGCTGAGAGTACTCATCTTGACACAAAAAA GCCAGGTTATAATGAGATCCCAGAGAGAGAAGAAAATGGTGGAAATACCATTGGAACTAGGGATGAAACT GCGAAAGAGGCAGATGCTGTTGATGTCAGCCTTGTAGAGGGCAGCAACGATATCATGGGTAGTACCAATT TTAAGGAGCTCCCTGGAAGAGAAGGAAACAGAGTGGATGCTGGCAGCCAAAATGCTCACCAAGGGAAGGT TGAGTTTCATTACCCTCCTGCACCCTCAAAAGAGAAAAGAAAAGAAGGCAGTAGTGATGCAGCTGAAAGT ACCAACTATAATGAAATTCCTAAAAATGGCAAAGGCAGTACCAGAAAGGGTGTAGATCATTCTAATAGGA ACCAAGCAACCTTAAATGAAAAACAAAGGTTTCCTAGTAAGGGCAAAAGTCAGGGCCTGCCCATTCCTTC TCGTGGTCTTGATAATGAAATCAAAAACGAAATGGATTCCTTTAATGGCCCCAGTCATGAGAATATAATA ACACATGGCAGAAAATATCATTATGTACCCCACAGACAAAATAATTCTACACGGAATAAGGGTATGCCAC AAGGGAAAGGCTCCTGGGGTAGACAACCCCATTCCAACAGGAGGTTTAGTTCCCGTAGAAGGGATGACAG TAGTGAGTCATCTGACAGTGGCAGTTCAAGTGAGAGCGATGGTGAC ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA >RG230031 representing NM_001184695
Red=Cloning site Green=Tags(s) MSIYPKSTGNKGFEDGDDAISKLHDQEEYGAALIRNNMQHIMGPVTAIKLLGEENKENTPRNVLNIIPAS MNYAKAHSKDKKKPQRDSQAQKSPVKSKSTHRIQHNIDYLKHLSKVKKIPSDFEGSGYTDLQERGDNDIS PFSGDGQPFKDIPGKGEATGPDLEGKDIQTGFAGPSEAESTHLDTKKPGYNEIPEREENGGNTIGTRDET AKEADAVDVSLVEGSNDIMGSTNFKELPGREGNRVDAGSQNAHQGKVEFHYPPAPSKEKRKEGSSDAAES TNYNEIPKNGKGSTRKGVDHSNRNQATLNEKQRFPSKGKSQGLPIPSRGLDNEIKNEMDSFNGPSHENII THGRKYHYVPHRQNNSTRNKGMPQGKGSWGRQPHSNRRFSSRRRDDSSESSDSGSSSESDGD TRTRPLE - GFP Tag - V |
Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
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ACCN | NM_001184695 |
ORF Size | 1236 bp |
OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
Reference Data | |
RefSeq | NM_001184695.2 |
RefSeq Size | 2113 bp |
RefSeq ORF | 1239 bp |
Locus ID | 56955 |
UniProt ID | Q9NQ76 |
Protein Families | Secreted Protein |
Gene Summary | This gene encodes a secreted calcium-binding phosphoprotein that belongs to the small integrin-binding ligand, N-linked glycoprotein (SIBLING) family of proteins. Members of this family are components of the extracellular matrix of bone and dentin and regulate bone mineralization. Deficiency of a similar protein in mouse results in increased bone mass. Mice lacking this gene are resistant to aging-related trabecular bone loss. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Mar 2014] |
Documents
Product Manuals |
FAQs |
SDS |
Resources
Other Versions
SKU | Description | Size | Price |
---|---|---|---|
RC230031 | MEPE (Myc-DDK-tagged)-Human matrix extracellular phosphoglycoprotein (MEPE), transcript variant 3 |
CNY 3,990.00 |
|
RC230031L3 | Lenti-ORF clone of MEPE (Myc-DDK-tagged)-Human matrix extracellular phosphoglycoprotein (MEPE), transcript variant 3 |
CNY 5,890.00 |
|
RC230031L4 | Lenti-ORF clone of MEPE (mGFP-tagged)-Human matrix extracellular phosphoglycoprotein (MEPE), transcript variant 3 |
CNY 5,890.00 |
|
SC328669 | MEPE (untagged)-Human matrix extracellular phosphoglycoprotein (MEPE) transcript variant 3 |
CNY 7,220.00 |