SEC23B (NM_001172746) Human Tagged ORF Clone

CAT＃: RG230476

TrueORF^®

SEC23B (tGFP-tagged) - Human Sec23 homolog B (S. cerevisiae) (SEC23B), transcript variant 5

ORF Plasmid: DDK tGFP

Datasheet

SDS

Plasmid Map

"NM_001172746" in other vectors (4)

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CNY 6,370.00

货期*

6周

规格

Product images

经常一起买 (5)

PS100010

pCMV6-AC-GFP, mammalian vector with C-terminal tGFP tag, 10ug

CNY 6,080.00

TA150041

Mouse monoclonal turboGFP antibody, clone OTI2H8

CNY 1,000.00

【特别福利】购一抗正装产品，免费领10ul抗体试用装

CC100003

DH5α Chemically Competent Cells (≥10^8 cfu/μg of pUC19 DNA)

CNY 1,280.00

PRIMER

Forward sequencing primer VP1.5, Reverse sequencing primer XL39, 100pmoles each

CNY 480.00

TA810159

SEC23B mouse monoclonal antibody,clone OTI8G4

CNY 1,999.00
CNY 2,700.00

【特别福利】购一抗正装产品，免费领10ul抗体试用装

Specifications

Product Data
Type	Human Tagged ORF Clone
Tag	TurboGFP
Synonyms	CDA-II; CDAII; CDAN2; CWS7; HEMPAS; hSec23B
Vector	pCMV6-AC-GFP
E. coli Selection	Ampicillin (100 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	ORF Nucleotide Sequence (showhide) >RG230476 representing NM_001172746 Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGCGACATACCTGGAGTTCATCCAGCAGAATGAAGAACGGGATGGTGTGCGTTTTAGTTGGAACGTGT GGCCTTCCAGCCGGCTGGAGGCTACAAGAATGGTTGTACCCCTGGCTTGTCTCCTTACTCCTTTGAAAGA ACGTCCAGACCTACCTCCTGTACAATATGAACCTGTGCTTTGCAGCAGGCCAACTTGTAAAGCTGTTCTC AACCCACTTTGTCAGGTTGATTATCGAGCAAAACTTTGGGCCTGTAATTTCTGTTTTCAAAGAAATCAGT TTCCTCCAGCTTATGGAGGCATATCTGAGGTGAATCAACCTGCCGAATTGATGCCCCAGTTTTCTACAAT TGAGTACGTGATACAGGAAGATGACCTTCAAGCACTCAAAGAGTCCCTGCAGATGTCCCTGAGTCTTCTT CCTCCAGATGCTCTGGTGGGTCTGATCACATTTGGAAGGATGGTGCAGGTTCATGAGCTAAGCTGTGAAG GAATCTCCAAAAGTTATGTCTTCCGAGGGACCAAGGATTTAACTGCAAAGCAAATACAGGATATGTTGGG CCTGACCAAGCCAGCCATGCCCATGCAGCAAGCACGACCTGCACAACCACAGGAGCACCCTTTTGCTTCA AGCAGATTTCTGCAGCCTGTTCACAAGATTGATATGAACCTCACTGATCTTCTTGGGGAGCTACAGAGGG ACCCATGGCCAGTAACTCAGGGGAAGAGACCTTTGCGATCCACTGGTGTGGCTTTGTCCATTGCTGTTGG CTTGCTGGAGGGCACTTTTCCAAACACAGGAGCCAGGATCATGCTGTTTACTGGAGGTCCCCCTACCCAA GGGCCTGGCATGGTGGTTGGAGATGAATTAAAGATTCCTATTCGTTCTTGGCATGATATTGAGAAAGATA ATGCACGATTCATGAAAAAGGCAACCAAGCACTATGAGATGCTTGCTAATCGAACAGCTGCAAATGGTCA CTGCATTGATATTTATGCTTGTGCCCTTGATCAAACTGGACTTTTGGAGATGAAGTGTTGTGCAAATCTT ACTGGAGGCTACATGGTAATGGGAGATTCTTTCAACACTTCTCTCTTCAAGCAGACATTCCAAAGAATCT TTACTAAAGATTTTAATGGAGATTTCCGAATGGCATTTGGTGCTACTTTGGACGTAAAGACCTCTCGGGA ACTGAAGATTGCAGGAGCCATTGGTCCATGCGTATCTCTGAATGTGAAAGGACCGTGTGTGTCAGAAAAT GAGCTTGGTGTTGGTGGCACGAGTCAGTGGAAAATCTGTGGCCTAGATCCTACATCTACACTTGGCATCT ATTTTGAAGTTGTCAATCAGCACAACACCCCGATCCCCCAAGGAGGCAGAGGAGCCATCCAGTTTGTCAC GCATTATCAGCACTCCAGCACCCAGAGACGCATCCGCGTGACCACCATCGCCCGAAATTGGGCAGATGTA CAGAGTCAGCTCAGGCACATAGAAGCAGCATTTGACCAGGAGGCTGCGGCAGTGTTGATGGCACGGCTTG GGGTGTTCCGAGCGGAGTCAGAGGAGGGGCCCGATGTGCTCCGGTGGCTGGACCGACAACTCATCCGACT GTGTCAAAAGTTTGGACAGTATAACAAAGAAGACCCCACTTCTTTTAGGTTATCAGATTCCTTTTCTCTA TATCCTCAGTTTATGTTCCATCTGAGAAGATCTCCATTTCTTCAAGTGTTTAACAACAGTCCTGATGAGT CGTCATATTACAGACATCATTTTGCCCGGCAGGACCTGACCCAGTCCCTCATCATGATCCAGCCCATTCT CTACTCTTACTCCTTTCATGGGCCACCAGAGCCAGTACTCTTGGATAGCAGCAGCATTCTAGCTGACAGA ATTTTGCTGATGGATACTTTCTTTCAAATTGTCATTTATCTTGGTGAGACCATAGCCCAGTGGCGTAAAG CTGGCTACCAGGACATGCCCGAGTATGAAAACTTCAAGCACCTTCTGCAGGCACCACTGGATGATGCTCA AGAAATTCTGCAAGCACGCTTCCCGATGCCACGTTACATCAACACGGAGCATGGAGGCAGTCAGGCTCGA TTCCTTTTGTCCAAAGTGAACCCATCTCAGACACACAATAACCTGTATGCTTGGGGACAGGAAACTGGAG CACCCATCCTAACTGATGATGTTAGCCTGCAGGTGTTCATGGACCATTTGAAGAAGCTGGCTGTCTCCAG TGCCTGT ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA Protein Sequence (showhide) >RG230476 representing NM_001172746 Red=Cloning site Green=Tags(s) MATYLEFIQQNEERDGVRFSWNVWPSSRLEATRMVVPLACLLTPLKERPDLPPVQYEPVLCSRPTCKAVL NPLCQVDYRAKLWACNFCFQRNQFPPAYGGISEVNQPAELMPQFSTIEYVIQEDDLQALKESLQMSLSLL PPDALVGLITFGRMVQVHELSCEGISKSYVFRGTKDLTAKQIQDMLGLTKPAMPMQQARPAQPQEHPFAS SRFLQPVHKIDMNLTDLLGELQRDPWPVTQGKRPLRSTGVALSIAVGLLEGTFPNTGARIMLFTGGPPTQ GPGMVVGDELKIPIRSWHDIEKDNARFMKKATKHYEMLANRTAANGHCIDIYACALDQTGLLEMKCCANL TGGYMVMGDSFNTSLFKQTFQRIFTKDFNGDFRMAFGATLDVKTSRELKIAGAIGPCVSLNVKGPCVSEN ELGVGGTSQWKICGLDPTSTLGIYFEVVNQHNTPIPQGGRGAIQFVTHYQHSSTQRRIRVTTIARNWADV QSQLRHIEAAFDQEAAAVLMARLGVFRAESEEGPDVLRWLDRQLIRLCQKFGQYNKEDPTSFRLSDSFSL YPQFMFHLRRSPFLQVFNNSPDESSYYRHHFARQDLTQSLIMIQPILYSYSFHGPPEPVLLDSSSILADR ILLMDTFFQIVIYLGETIAQWRKAGYQDMPEYENFKHLLQAPLDDAQEILQARFPMPRYINTEHGGSQAR FLLSKVNPSQTHNNLYAWGQETGAPILTDDVSLQVFMDHLKKLAVSSAC TRTRPLE - GFP Tag - V
Restriction Sites	SgfI-MluI Cloning Scheme for this gene Plasmid Map
ACCN	NM_001172746
ORF Size	2247 bp
OTI Disclaimer	The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation	This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq	NM_001172746.3
RefSeq Size	3364 bp
RefSeq ORF	2250 bp
Locus ID	10483
Gene Summary	The protein encoded by this gene is a member of the SEC23 subfamily of the SEC23/SEC24 family, which is involved in vesicle trafficking. The encoded protein has similarity to yeast Sec23p component of COPII. COPII is the coat protein complex responsible for vesicle budding from the ER. The function of this gene product has been implicated in cargo selection and concentration. Multiple alternatively spliced transcript variants have been identified in this gene. [provided by RefSeq, Feb 2010]

*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

Documents

Product Manuals
TrueORFs: Open Reading Frame Clones - 10 ug in PrecisionShuttle Vector System pLenti-ORF destination vector Transfection - TurboFectin 8.0 Transfection Reagent Plasmid Preparation - PowerPrep Plasmid Purification Kits
FAQs
TrueORF Clones/PrecisionShuttle Organelle Marker Transfection Reagent
SDS
TrueORF SDS

Resources

cDNA 克隆相关资源
Video Tutorial: Clone Selection Guide Product Video: What is TrueORF GOLD and why it is the most popular type of clone Video Tutorial: How to make Lentivirus particles Video Tutorial: Is the Lentivirus Safe Product Brochure: cDNA clones $98 Clones. Download the List of 15,000 Protocol: Lentivirus packaging

Other Versions

SKU	Description	Size	Price
RC230476	SEC23B (Myc-DDK-tagged)-Human Sec23 homolog B (S. cerevisiae) (SEC23B), transcript variant 5	10 ug 产品选项验证失败所有选项都是必需的	CNY 5,800.00
RC230476L3	Lenti-ORF clone of SEC23B (Myc-DDK-tagged)-Human Sec23 homolog B (S. cerevisiae) (SEC23B), transcript variant 5	10 ug 产品选项验证失败所有选项都是必需的	CNY 7,700.00
RC230476L4	Lenti-ORF clone of SEC23B (mGFP-tagged)-Human Sec23 homolog B (S. cerevisiae) (SEC23B), transcript variant 5	10 ug 产品选项验证失败所有选项都是必需的	CNY 7,700.00
SC329114	SEC23B (untagged)-Human Sec23 homolog B (S. cerevisiae) (SEC23B) transcript variant 5	10 ug 产品选项验证失败所有选项都是必需的	CNY 14,350.00

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