PGC1 beta (PPARGC1B) (NM_001172698) Human Tagged ORF Clone
CAT#: RG230600
- TrueORF®
PPARGC1B (tGFP-tagged) - Human peroxisome proliferator-activated receptor gamma, coactivator 1 beta (PPARGC1B), transcript variant 2
ORF Plasmid: DDK
"NM_001172698" in other vectors (4)
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CNY 4,370.00
Specifications
Product Data | |
Type | Human Tagged ORF Clone |
Tag | TurboGFP |
Synonyms | ERRL1; PERC; PGC-1(beta); PGC1B |
Vector | pCMV6-AC-GFP |
E. coli Selection | Ampicillin (100 ug/mL) |
Mammalian Cell Selection | Neomycin |
Sequence Data |
>RG230600 representing NM_001172698
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGCGGGGAACGACTGCGGCGCGCTGCTGGACGAAGAGCTCTCCTCCTTCTTCCTCAACTATCTCGCTG ACACGCAGGGTGGAGGGTCCGGGGAGGAGCAACTCTATGCTGACTTTCCAGAACTTGACCTCTCCCAGCT GGATGCCAGCGACTTTGACTCGGCCACCTGCTTTGGGGAGCTGCAGTGGTGCCCAGAGAACTCAGAGACT GAACCCAACCAGTACAGCCCCGATGACTCCGAGCTCTTCCAGATTGACAGTGAGAATGAGGCCCTCCTGG CAGAGCTCACCAAGACCCTGGATGACATCCCTGAAGATGACGTGGGTCTGGCTGCCTTCCCAGCCCTGGA TGGTGGAGACGCTCTATCATGCACCTCAGCTTCGCCTGCCCCCTCATCTGCACCCCCCAGCCCTGCCCCG GAGAAGCCCTCGGCCCCAGCCCCTGAGGTGGACGAGCTCTCACTGGCGGACAGCACCCAAGACAAGAAGG CTCCCATGATGCAGTCTCAGAGCCGAAGTTGTACAGAACTACATAAGCACCTCACCTCGGCACAGTGCTG CCTGCAGGATCGGGGTCTGCAGCCACCATGCCTCCAGAGTCCCCGGCTCCCTGCCAAGGAGGACAAGGAG CCGGGTGAGGACTGCCCGAGCCCCCAGCCAGCTCCAGCCTCTCCCCGGGACTCCCTAGCTCTGGGCAGGG CAGACCCCGGTGCCCCGGTTTCCCAGGAAGACATGCAGGCGATGGTGCAACTCATACGCTACATGCACAC CTACTGCCTCCCCCAGAGGAAGCTGCCCCCACAGACCCCTGAGCCACTCCCCAAGGCCTGCAGCAACCCC TCCCAGCAGGTCAGATCCCGGCCCTGGTCCCGGCACCACTCCAAAGCCTCCTGGGCTGAGTTCTCCATTC TGAGGGAACTTCTGGCTCAAGACGTGCTCTGTGATGTCAGCAAACCCTACCGTCTGGCCACGCCTGTTTA TGCCTCCCTCACACCTCGGTCAAGGCCCAGGCCCCCCAAAGACAGTCAGGCCTCCCCTGGTCGCCCGTCC TCGGTGGAGGAGGTAAGGATCGCAGCTTCACCCAAGAGCACCGGGCCCAGACCAAGCCTGCGCCCACTGC GGCTGGAGGTGAAAAGGGAGGTCCGCCGGCCTGCCAGACTGCAGCAGCAGGAGGAGGAAGACGAGGAAGA AGAGGAGGAGGAAGAGGAAGAAGAAAAAGAGGAGGAGGAGGAGTGGGGCAGGAAAAGGCCAGGCCGAGGC CTGCCATGGACGAAGCTGGGGAGGAAGCTGGAGAGCTCTGTGTGCCCCGTGCGGCGTTCTCGGAGACTGA ACCCTGAGCTGGGCCCCTGGCTGACATTTGCAGATGAGCCGCTGGTCCCCTCGGAGCCCCAAGGTGCTCT GCCCTCACTGTGCCTGGCTCCCAAGGCCTACGACGTAGAGCGGGAGCTGGGCAGCCCCACGGACGAGGAC AGTGGCCAAGACCAGCAGCTCCTACGGGGACCCCAGATCCCTGCCCTGGAGAGCCCCTGTGAGAGTGGGT GTGGGGACATGGATGAGGACCCCAGCTGCCCGCAGCTCCCTCCCAGAGACTCTCCCAGGTGCCTCATGCT GGCCTTGTCACAAAGCGACCCAACTTTTGGCAAGAAGAGCTTTGAGCAGACCTTGACAGTGGAGCTCTGT GGCACAGCAGGACTCACCCCACCCACCACACCACCGTACAAGCCCACAGAGGAGGATCCCTTCAAACCAG ACATCAAGCATAGTCTAGGCAAAGAAATAGCTCTCAGCCTCCCCTCCCCTGAGGGCCTCTCACTCAAGGC CACCCCAGGGGCTGCCCACAAGCTGCCAAAGAAGCACCCAGAGCGAAGTGAGCTCCTGTCCCACCTGCGA CATGCCACAGCCCAGCCAGCCTCCCAGGCTGGCCAGAAGCGTCCCTTCTCCTGTTCCTTTGGAGACCATG ACTACTGCCAGGTGCTCCGACCAGAAGGCGTCCTGCAAAGGAAGGTGCTGAGGTCCTGGGAGCCGTCTGG GGTTCACCTTGAGGACTGGCCCCAGCAGGGTGCCCCTTGGGCTGAGGCACAGGCCCCTGGCAGGGAGGAA GACAGAAGCTGTGATGCTGGCGCCCCACCCAAGGACAGCACGCTGCTGAGAGACCATGAGATCCGTGCCA GCCTCACCAAACACTTTGGGCTGCTGGAGACCGCCCTGGAGGAGGAAGACCTGGCCTCCTGCAAGAGCCC TGAGTATGACACTGTCTTTGAAGACAGCAGCAGCAGCAGCGGCGAGAGCAGCTTCCTCCCAGAGGAGGAA GAGGAAGAAGGGGAGGAGGAGGAGGAGGACGATGAAGAAGAGGACTCAGGGGTCAGCCCCACTTGCTCTG ACCACTGCCCCTACCAGAGCCCACCAAGCAAGGCCAACCGGCAGCTCTGTTCCCGCAGCCGCTCAAGCTC TGGCTCTTCACCCTGCCACTCCTGGTCACCAGCCACTCGAAGGAACTTCAGATGTGAGAGCAGAGGGCCG TGTTCAGACAGAACGCCAAGCATCCGGCACGCCAGGAAGCGGCGGGAAAAGGCCATTGGGGAAGGCCGCG TGGTGTACATTCAAAATCTCTCCAGCGACATGAGCTCCCGAGAGCTGAAGAGGCGCTTTGAAGTGTTTGG TGAGATTGAGGAGTGCGAGGTGCTGACAAGAAATAGGAGAGGCGAGAAGTACGGCTTCATCACCTACCGG TGTTCTGAGCACGCGGCCCTCTCTTTGACAAAGGGCGCTGCCCTGAGGAAGCGCAACGAGCCCTCCTTCC AGCTGAGCTACGGAGGGCTCCGGCACTTCTGCTGGCCCAGATACACTGACTACGATTCCAATTCAGAAGA GGCCCTTCCTGCGTCAGGGAAAAGCAAGTATGAAGCCATGGATTTTGACAGCTTACTGAAAGAGGCCCAG CAGAGCCTGCAT ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA >RG230600 representing NM_001172698
Red=Cloning site Green=Tags(s) MAGNDCGALLDEELSSFFLNYLADTQGGGSGEEQLYADFPELDLSQLDASDFDSATCFGELQWCPENSET EPNQYSPDDSELFQIDSENEALLAELTKTLDDIPEDDVGLAAFPALDGGDALSCTSASPAPSSAPPSPAP EKPSAPAPEVDELSLADSTQDKKAPMMQSQSRSCTELHKHLTSAQCCLQDRGLQPPCLQSPRLPAKEDKE PGEDCPSPQPAPASPRDSLALGRADPGAPVSQEDMQAMVQLIRYMHTYCLPQRKLPPQTPEPLPKACSNP SQQVRSRPWSRHHSKASWAEFSILRELLAQDVLCDVSKPYRLATPVYASLTPRSRPRPPKDSQASPGRPS SVEEVRIAASPKSTGPRPSLRPLRLEVKREVRRPARLQQQEEEDEEEEEEEEEEEKEEEEEWGRKRPGRG LPWTKLGRKLESSVCPVRRSRRLNPELGPWLTFADEPLVPSEPQGALPSLCLAPKAYDVERELGSPTDED SGQDQQLLRGPQIPALESPCESGCGDMDEDPSCPQLPPRDSPRCLMLALSQSDPTFGKKSFEQTLTVELC GTAGLTPPTTPPYKPTEEDPFKPDIKHSLGKEIALSLPSPEGLSLKATPGAAHKLPKKHPERSELLSHLR HATAQPASQAGQKRPFSCSFGDHDYCQVLRPEGVLQRKVLRSWEPSGVHLEDWPQQGAPWAEAQAPGREE DRSCDAGAPPKDSTLLRDHEIRASLTKHFGLLETALEEEDLASCKSPEYDTVFEDSSSSSGESSFLPEEE EEEGEEEEEDDEEEDSGVSPTCSDHCPYQSPPSKANRQLCSRSRSSSGSSPCHSWSPATRRNFRCESRGP CSDRTPSIRHARKRREKAIGEGRVVYIQNLSSDMSSRELKRRFEVFGEIEECEVLTRNRRGEKYGFITYR CSEHAALSLTKGAALRKRNEPSFQLSYGGLRHFCWPRYTDYDSNSEEALPASGKSKYEAMDFDSLLKEAQ QSLH TRTRPLE - GFP Tag - V |
Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
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ACCN | NM_001172698 |
ORF Size | 2952 bp |
OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
Reference Data | |
RefSeq | NM_001172698.2 |
RefSeq Size | 10525 bp |
RefSeq ORF | 2955 bp |
Locus ID | 133522 |
UniProt ID | Q86YN6 |
Protein Families | Druggable Genome, Transcription Factors |
Gene Summary | The protein encoded by this gene stimulates the activity of several transcription factors and nuclear receptors, including estrogen receptor alpha, nuclear respiratory factor 1, and glucocorticoid receptor. The encoded protein may be involved in fat oxidation, non-oxidative glucose metabolism, and the regulation of energy expenditure. This protein is downregulated in prediabetic and type 2 diabetes mellitus patients. Certain allelic variations in this gene increase the risk of the development of obesity. Three transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Mar 2010] |
Documents
Product Manuals |
FAQs |
SDS |
Resources
Other Versions
SKU | Description | Size | Price |
---|---|---|---|
RC230600 | PPARGC1B (Myc-DDK-tagged)-Human peroxisome proliferator-activated receptor gamma, coactivator 1 beta (PPARGC1B), transcript variant 2 |
CNY 3,990.00 |
|
RC230600L3 | Lenti-ORF clone of PPARGC1B (Myc-DDK-tagged)-Human peroxisome proliferator-activated receptor gamma, coactivator 1 beta (PPARGC1B), transcript variant 2 |
CNY 5,890.00 |
|
RC230600L4 | Lenti-ORF clone of PPARGC1B (mGFP-tagged)-Human peroxisome proliferator-activated receptor gamma, coactivator 1 beta (PPARGC1B), transcript variant 2 |
CNY 5,890.00 |
|
SC329238 | PPARGC1B (untagged)-Human peroxisome proliferator-activated receptor gamma coactivator 1 beta (PPARGC1B) transcript variant 2 |
CNY 7,220.00 |
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