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SLC4A10 (NM_001178016) Human Tagged ORF Clone
CAT＃: RG230649
- TrueORF®
SLC4A10 (tGFP-tagged) - Human solute carrier family 4, sodium bicarbonate transporter, member 10 (SLC4A10), transcript variant 3
ORF Plasmid: DDK
"NM_001178016" in other vectors (4)
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CNY 11,970.00
Product images
Specifications
| Product Data | |
| Type | Human Tagged ORF Clone |
| Tag | TurboGFP |
| Synonyms | NBCn2; NCBE |
| Vector | pCMV6-AC-GFP |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>RG230649 representing NM_001178016
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGCAGTCTTTAGGGGTTTCTGGAAATAGAAAGGTCATGCAGTCTGGAACCTGTGAGCCTTTTCAATCTC TAAGTCATCAGAGAAATGATGAAGAAGCAGTTGTGGATAGAGGTGGAACTCGTTCTATTCTCAAAACACA CTTTGAGAAAGAAGATTTAGAAGGTCATCGAACACTATTTATTGGAGTACATGTGCCCTTGGGAGGAAGA AAAAGCCATCGACGTCACAGGCATCGTGGTCATAAACACAGAAAGAGAGACAGAGAAAGAGATTCAGGAT TAGAGGATGGAAGGGAGTCACCTTCTTTTGACACCCCATCACAGAGGGTACAGTTTATTCTTGGAACCGA GGATGATGACGAGGAACACATTCCTCATGACCTTTTCACAGAACTGGATGAGATTTGTTGGCGTGAAGGT GAGGACGCTGAGTGGCGAGAAACAGCCAGGTGGTTGAAGTTTGAAGAAGATGTGGAAGATGGAGGAGAAA GGTGGAGCAAGCCTTATGTGGCTACTCTTTCATTGCACAGCTTGTTTGAATTGAGAAGTTGTATTCTGAA TGGAACTGTGTTGCTGGACATGCATGCCAACACTTTAGAAGAAATTGCAGATATGGTTCTTGACCAACAA GTGAGCTCAGGTCAGCTGAATGAAGATGTACGCCATAGGGTCCATGAGGCATTGATGAAACAGCATCATC ATCAGAATCAGAAAAAACTCACCAACAGGATTCCCATTGTTCGTTCCTTTGCTGATATTGGCAAGAAACA GTCAGAACCAAATTCCATGGACAAAAATGCAGGTCAGGTTGTTTCTCCTCAGTCTGCTCCAGCCTGTGTT GAAAATAAAAATGATGTTAGCAGAGAAAACAGCACTGTTGACTTTAGCAAGGTTGATCTGCATTTTATGA AAAAGATTCCTCCAGGTGCTGAAGCATCGAACATCTTAGTGGGAGAACTGGAGTTCTTGGATCGAACAGT AGTTGCGTTTGTCAGGTTGTCTCCAGCTGTATTGCTTCAAGGACTGGCTGAAGTCCCAATCCCAACCAGA TTTTTGTTCATTCTTCTGGGACCCCTGGGAAAGGGTCAACAGTACCATGAGATTGGCAGATCAATTGCAA CCCTAATGACAGATGAGGTATTTCATGATGTTGCCTATAAAGCTAAAGATCGTAATGACTTGGTATCAGG AATTGATGAGTTTCTGGATCAGGTTACTGTTCTCCCTCCTGGAGAATGGGATCCAAGCATTCGAATAGAG CCTCCCAAAAATGTTCCTTCCCAGGAGAAGAGGAAGATTCCTGCTGTACCAAATGGAACAGCAGCTCATG GGGAAGCAGAGCCCCACGGAGGACATAGTGGACCTGAACTCCAGCGAACTGGAAGGATTTTTGGGGGACT TATTTTAGATATCAAAAGAAAAGCTCCATACTTCTGGAGTGACTTCAGAGATGCTTTCAGCCTGCAGTGC TTAGCATCTTTTCTATTTCTCTACTGCGCGTGTATGTCTCCTGTCATCACGTTTGGAGGACTGCTGGGAG AAGCAACTGAAGGGCGTATAAGTGCAATTGAATCTCTCTTTGGAGCATCCATGACCGGGATAGCCTATTC TCTCTTTGGTGGACAGCCTCTTACCATATTAGGCAGTACAGGACCAGTTTTGGTGTTTGAAAAGATTTTG TTTAAATTTTGCAAAGAATATGGGCTGTCATACCTATCTTTAAGAGCTAGCATTGGACTTTGGACTGCAA CTCTATGTATCATACTTGTGGCCACAGATGCTAGTTCCCTTGTCTGCTACATCACTCGGTTTACTGAAGA AGCTTTTGCTTCCCTGATTTGCATCATTTTCATTTATGAGGCCCTGGAGAAGTTGTTTGAACTCAGTGAA GCATATCCAATCAACATGCATAATGATCTGGAACTGCTGACACAATACTCGTGTAACTGTGTGGAACCGC ATAATCCCAGCAATGGCACATTGAAGGAATGGAGGGAATCCAATATTTCTGCCTCTGACATAATTTGGGA GAACCTAACTGTGTCAGAATGCAAATCATTGCATGGAGAGTATGTTGGACGGGCCTGTGGCCATGATCAC CCATATGTTCCAGATGTTCTATTTTGGTCTGTGATCCTGTTCTTTTCCACAGTTACTCTGTCAGCCACCC TGAAGCAGTTCAAGACTAGCAGATATTTTCCAACCAAGGTTCGATCCATAGTGAGTGACTTTGCTGTCTT TCTTACAATTCTGTGTATGGTTTTAATTGACTATGCCATTGGGATCCCATCTCCAAAACTACAAGTACCA AGTGTTTTCAAGCCCACTAGAGATGATCGTGGCTGGTTTGTTACGCCTTTAGGTCCAAACCCATGGTGGA CAGTAATAGCTGCTATAATTCCAGCTCTGCTTTGTACTATTCTAATTTTTATGGACCAACAGATTACAGC TGTCATCATCAACAGGAAAGAGCATAAGCTAAAGAAAGGTTGTGGGTACCATCTGGACCTATTAATGGTG GCTGTCATGCTCGGTGTATGCTCCATCATGGGCCTGCCATGGTTTGTGGCTGCCACAGTCCTCTCCATCA CTCATGTCAATAGCCTAAAACTGGAATCAGAATGCTCAGCTCCAGGAGAACAACCCAAATTTCTCGGCAT TCGGGAGCAAAGGGTTACTGGGCTTATGATTTTTATTCTTATGGGTTCATCAGTCTTTATGACCAGTATT CTGAAGTTTATTCCCATGCCAGTGCTATATGGAGTGTTTCTTTATATGGGTGCTTCATCTCTAAAGGGAA TTCAGTTCTTTGATAGGATAAAGCTCTTCTGGATGCCGGCAAAACATCAACCAGATTTTATATACCTAAG GCACGTACCGCTTCGAAAAGTGCATCTCTTCACAATTATTCAGATGAGTTGCCTTGGCCTTTTGTGGATA ATAAAAGTTTCAAGAGCTGCTATTGTCTTTCCCATGATGGTGTTAGCCCTGGTATTTGTAAGAAAGTTGA TGGACTTGTTGTTCACGAAGCGGGAACTCAGCTGGTTGGATGATTTGATGCCCGAGAGTAAGAAAAAGAA ACTGGAAGATGCTGAAAAAGAAGAAGAACAAAGTATGCTAGCTATGGAAGATGAGGGCACAGTACAACTC CCATTGGAAGGGCACTATAGAGATGATCCATCTGTGATCAATATATCTGATGAAATGTCAAAGACTGCCT TGTGGAGGAACCTTCTGATTACTGCCGATAACTCAAAAGATAAGGAGTCAAGCTTTCCTTCCAAAAGCTC CCCTTCC ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA >RG230649 representing NM_001178016
Red=Cloning site Green=Tags(s) MQSLGVSGNRKVMQSGTCEPFQSLSHQRNDEEAVVDRGGTRSILKTHFEKEDLEGHRTLFIGVHVPLGGR KSHRRHRHRGHKHRKRDRERDSGLEDGRESPSFDTPSQRVQFILGTEDDDEEHIPHDLFTELDEICWREG EDAEWRETARWLKFEEDVEDGGERWSKPYVATLSLHSLFELRSCILNGTVLLDMHANTLEEIADMVLDQQ VSSGQLNEDVRHRVHEALMKQHHHQNQKKLTNRIPIVRSFADIGKKQSEPNSMDKNAGQVVSPQSAPACV ENKNDVSRENSTVDFSKVDLHFMKKIPPGAEASNILVGELEFLDRTVVAFVRLSPAVLLQGLAEVPIPTR FLFILLGPLGKGQQYHEIGRSIATLMTDEVFHDVAYKAKDRNDLVSGIDEFLDQVTVLPPGEWDPSIRIE PPKNVPSQEKRKIPAVPNGTAAHGEAEPHGGHSGPELQRTGRIFGGLILDIKRKAPYFWSDFRDAFSLQC LASFLFLYCACMSPVITFGGLLGEATEGRISAIESLFGASMTGIAYSLFGGQPLTILGSTGPVLVFEKIL FKFCKEYGLSYLSLRASIGLWTATLCIILVATDASSLVCYITRFTEEAFASLICIIFIYEALEKLFELSE AYPINMHNDLELLTQYSCNCVEPHNPSNGTLKEWRESNISASDIIWENLTVSECKSLHGEYVGRACGHDH PYVPDVLFWSVILFFSTVTLSATLKQFKTSRYFPTKVRSIVSDFAVFLTILCMVLIDYAIGIPSPKLQVP SVFKPTRDDRGWFVTPLGPNPWWTVIAAIIPALLCTILIFMDQQITAVIINRKEHKLKKGCGYHLDLLMV AVMLGVCSIMGLPWFVAATVLSITHVNSLKLESECSAPGEQPKFLGIREQRVTGLMIFILMGSSVFMTSI LKFIPMPVLYGVFLYMGASSLKGIQFFDRIKLFWMPAKHQPDFIYLRHVPLRKVHLFTIIQMSCLGLLWI IKVSRAAIVFPMMVLALVFVRKLMDLLFTKRELSWLDDLMPESKKKKLEDAEKEEEQSMLAMEDEGTVQL PLEGHYRDDPSVINISDEMSKTALWRNLLITADNSKDKESSFPSKSSPS TRTRPLE - GFP Tag - V |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_001178016 |
| ORF Size | 3297 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_001178016.1, NP_001171487.1 |
| RefSeq Size | 5717 bp |
| RefSeq ORF | 3300 bp |
| Locus ID | 57282 |
| UniProt ID | Q6U841 |
| Protein Families | Druggable Genome, Transmembrane |
| Gene Summary | This gene belongs to a small family of sodium-coupled bicarbonate transporters (NCBTs) that regulate the intracellular pH of neurons, the secretion of bicarbonate ions across the choroid plexus, and the pH of the brain extracellular fluid. The protein encoded by this gene was initially identified as a sodium-driven chloride bicarbonate exchanger (NCBE) though there is now evidence that its sodium/bicarbonate cotransport activity is independent of any chloride ion countertransport under physiological conditions. This gene is now classified as a member A10 of the SLC4 family of transmembrane solute carriers. Alternative splicing results in multiple transcript variants encoding distinct isoforms.[provided by RefSeq, May 2010] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC230649 | SLC4A10 (Myc-DDK-tagged)-Human solute carrier family 4, sodium bicarbonate transporter, member 10 (SLC4A10), transcript variant 3 |
CNY 10,930.00 |
|
| RC230649L3 | Lenti-ORF clone of SLC4A10 (Myc-DDK-tagged)-Human solute carrier family 4, sodium bicarbonate transporter, member 10 (SLC4A10), transcript variant 3 |
CNY 12,830.00 |
|
| RC230649L4 | Lenti-ORF clone of SLC4A10 (mGFP-tagged)-Human solute carrier family 4, sodium bicarbonate transporter, member 10 (SLC4A10), transcript variant 3 |
CNY 12,830.00 |
|
| SC329287 | SLC4A10 (untagged)-Human solute carrier family 4 sodium bicarbonate transporter member 10 (SLC4A10) transcript variant 3 |
CNY 21,280.00 |
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