Dermokine (DMKN) (NM_001190349) Human Tagged ORF Clone
CAT#: RG231189
- TrueORF®
DMKN (tGFP-tagged) - Human dermokine (DMKN), transcript variant 10. Note: ORF is codon optimized
ORF Plasmid: DDK
"NM_001190349" in other vectors (3)
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CNY 4,370.00
Specifications
Product Data | |
Type | Human Tagged ORF Clone |
Tag | TurboGFP |
Synonyms | UNQ729; ZD52F10 |
Vector | pCMV6-AC-GFP |
E. coli Selection | Ampicillin (100 ug/mL) |
Mammalian Cell Selection | Neomycin |
Sequence Data |
>RG231189 ORF sequence, codon optimized.
Due to the complexity of NM_001190349, the ORF clone is codon optimized for mammalian Expression. The nucleotide sequence differs from the reference sequence, yet the amino acid sequence remains identical. Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGAAATTCCAAGGCCCCTTGGCCTGTCTGCTGCTTGCGCTTTGCCTTGGATCCGGGGAAGCGGGACCCT TGCAGTCCGGGGAGGAGAGCACAGGCACCAACATAGGCGAAGCTCTGGGGCACGGCTTGGGCGATGCACT GAGTGAGGGAGTCGGGAAAGCTATTGGAAAAGAAGCTGGCGGTGCCGCCGGCTCAAAAGTCTCCGAAGCC CTCGGTCAAGGGACAAGAGAGGCAGTTGGTACTGGGGTTCGCCAGGTCCCGGGGTTTGGGGTGGCAGACG CGCTGGGTAACAGAGTCGGGGAGGCTGCACACGCCCTCGGCAATACGGGGCACGAAATAGGGAGACAGGC CGAAGACGTTATTCGCCATGGCGCGGATGCCGTCAGAGGGAGCTGGCAAGGCGTCCCGGGGCACAATGGA GCCTGGGAGACATCAGGGGGCCATGGCATCTTCGGCAGTCAGGGGGGCCTTGGGGGACAGGGGCAGGGTA ATCCTGGGGGCCTCGGAACACCCTGGGTCCATGGATACCCGGGGAACTCTGCCGGGTCATTTGGTATGAA CCCGCAGGGCGCACCATGGGGCCAAGGCGGCAATGGAGGCCCTCCAAACTTCGGCACCAATACCCAGGGC GCCGTTGCCCAGCCCGGATACGGAAGCGTCAGAGCGTCCAACCAAAATGAGGGGTGCACAAACCCACCTC CATCAGGAAGCGGAGGCGGCTCATCCAACTCCGGCGGGGGGTCAGGGTCCCAATCTGGCTCCAGCGGCTC CGGATCTAACGGGGATAACAATAACGGGTCTAGCTCCGGCGGGAGTAGCTCCGGGTCTTCCTCAGGGGGG TCAAGCGGCGGCTCATCCGGCGGATCCTCTGGGAATAGTGGAGGCAGTAGGGGGGACTCTGGCTCCGAAA GCTCATGGGGCTCATCAACTGGAAGCAGCAGCGGAAATCACGGGGGCTCCGGTGGCGGCAACGGCCATAA GCCCGGAAACTCCGAAACATCCCCTGGCATGTTCAACTTCGACACCTTTTGGAAGAACTTTAAAAGCAAG CTTGGATTCATAAATTGGGACGCCATCAATAAGGATCAACGATCCTCTCGGATCCCA ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA >RG231189 representing NM_001190349
Red=Cloning site Green=Tags(s) MKFQGPLACLLLALCLGSGEAGPLQSGEESTGTNIGEALGHGLGDALSEGVGKAIGKEAGGAAGSKVSEA LGQGTREAVGTGVRQVPGFGVADALGNRVGEAAHALGNTGHEIGRQAEDVIRHGADAVRGSWQGVPGHNG AWETSGGHGIFGSQGGLGGQGQGNPGGLGTPWVHGYPGNSAGSFGMNPQGAPWGQGGNGGPPNFGTNTQG AVAQPGYGSVRASNQNEGCTNPPPSGSGGGSSNSGGGSGSQSGSSGSGSNGDNNNGSSSGGSSSGSSSGG SSGGSSGGSSGNSGGSRGDSGSESSWGSSTGSSSGNHGGSGGGNGHKPGNSETSPGMFNFDTFWKNFKSK LGFINWDAINKDQRSSRIP TRTRPLE - GFP Tag - V |
Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
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ACCN | NM_001190349 |
ORF Size | 1107 bp |
OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
Reference Data | |
RefSeq | NM_001190349.1, NP_001177278.1 |
RefSeq Size | 1420 bp |
RefSeq ORF | 1110 bp |
Locus ID | 93099 |
UniProt ID | Q6E0U4 |
Gene Summary | This gene is upregulated in inflammatory diseases, and it was first observed as expressed in the differentiated layers of skin. The most interesting aspect of this gene is the differential use of promoters and terminators to generate isoforms with unique cellular distributions and domain components. Alternatively spliced transcript variants encoding different isoforms have been identified for this gene. [provided by RefSeq, Jun 2010] |
Documents
Product Manuals |
FAQs |
SDS |
Resources
Other Versions
SKU | Description | Size | Price |
---|---|---|---|
RC231189 | DMKN (Myc-DDK-tagged)-Human dermokine (DMKN), transcript variant 10. Note: ORF is codon optimized |
CNY 3,656.00 |
|
RC231189L3 | Lenti-ORF clone of DMKN (Myc-DDK-tagged)-Human dermokine (DMKN), transcript variant 10. Note: ORF is codon optimized |
CNY 5,890.00 |
|
RC231189L4 | Lenti-ORF clone of DMKN (mGFP-tagged)-Human dermokine (DMKN), transcript variant 10. Note: ORF is codon optimized |
CNY 5,890.00 |
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