TAF1C (NM_001243160) Human Tagged ORF Clone

CAT#: RG232641

  • TrueORF®

TAF1C (tGFP-tagged) - Homo sapiens TATA box binding protein (TBP)-associated factor, RNA polymerase I, C, 110kDa (TAF1C), transcript variant 7

ORF Plasmid: DDK tGFP



  "NM_001243160" in other vectors (2)

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CNY 4,370.00


货期*
4周

规格
    • 10 ug

Product images

经常一起买 (5)
pCMV6-AC-GFP, mammalian vector with C-terminal tGFP tag, 10ug
    • 10 ug

CNY 6,080.00


Mouse monoclonal turboGFP antibody, clone OTI2H8
    • 100 ul

CNY 1,000.00


DH5α Chemically Competent Cells (≥10^8 cfu/μg of pUC19 DNA)
    • 5 x 200 ul

CNY 1,280.00


Forward sequencing primer VP1.5, Reverse sequencing primer XL39, 100pmoles each
    • 100 pmol

CNY 480.00


TAF1C Rabbit monoclonal Antibody
    • 100 ul

CNY 1,999.00
CNY 3,280.00

Specifications

Product Data
Type Human Tagged ORF Clone
Tag TurboGFP
Synonyms MGC:39976; SL1; TAFI95; TAFI110
Vector pCMV6-AC-GFP
E. coli Selection Ampicillin (100 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
>RG232641 representing NM_001243160
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGCTGAAGTGGAACCATGGCCTCCCCTCCCCGCTCCTGCTGGCCCGACTGCTGCCTCCGCCCCGGCCCA
GCTGCGTGCAGCCCCTGCTCCTCGGAGGCCAGGGTGGGCAGCTGCAGCTGCTGCACCTGGCAGAAGGGGC
GTCGGTGCCCCGCCTGGCAGGCCCCCCCCAGTCTCTTCCTTCCAGGATCGACTCCCTCCCTGCATTTCCT
CTGCTGGAGCCTAAGATCCAGTGGCGGCTGCAGGAGCGCCTGAAAGCACCGACCATAGGTCTGGCTGCCG
TCGTCCCGCCCTTGCCCTCAGCGCCCACACCAGGCCTGGTGCTCTTCCAGCTCTCGGCGGCGGGAGATGT
CTTCTACCAGCAGCTCCGCCCCCAGGTGGACTCCAGCCTCCGCAGAGATGCTGGGCCTCCTGGCGACACC
CAACCTGACTGCCATGCCCCCACAGCTTCCTGGACCTCCCAGGACACTGCCGGCTGCAGCCAGTGGCTGA
AGGCCCTGCTAAAAGTGCCCCTGGCTCCTCCTGTGTGGACAGCACCCACCTTCACCCACCGCCAGATGCT
GGGCAGCACAGAGCTGCGGAGGGAGGAAGAGGAAGGGCAGCGGCTGGGTGTGCTCCGCAAGGCCATGGCC
CGAGGGCAGCTCCTGCTGCAGAGAGACCTGGGCTCCCTCCCTGCGGCAGAGCCACCCCCTGCACCCGAGT
CAGGCCTAGAGGACAAGCTCAGTGAGCGCCTGGGGGAAGCCTGGGCAGGCCGAGGGGCTGCCTGGTGGGA
GAGGCAGCAGGGCAGGACCTCGGAGCCCGGGAGACAGACCAGGCGGCCCAAGCGCCGGACCCAGCTGTCC
AGCAGCTTTTCGCTCAGTGGCCATGTGGATCCCTCAGAGGACACCAGCTCCCCTCATAGCCCTGAGTGGC
CACCTGCTGATGCTCTGCCCCTGCCCCCCACGACCCCGCCCTCCCAGGAGTTGACTCCGGATGCATGCGC
CCAGGGCGTCCCATCAGAGCAGCGGCAGATGCTCCGTGACTACATGGCCAAGCTACCACCCCAGAGGGAC
ACCCCAGGCTGTGCCACCACACCTCCCCACTCCCAGGCCTCCAGCGTCCGGGCCACTCGCTCCCAGCAGC
ACACACCCGTCCTCTCTAGCTCTCAGCCCCTCCGGAAGAAGCCTCGAATGGGCTTC


ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
>RG232641 representing NM_001243160
Red=Cloning site Green=Tags(s)

MLKWNHGLPSPLLLARLLPPPRPSCVQPLLLGGQGGQLQLLHLAEGASVPRLAGPPQSLPSRIDSLPAFP
LLEPKIQWRLQERLKAPTIGLAAVVPPLPSAPTPGLVLFQLSAAGDVFYQQLRPQVDSSLRRDAGPPGDT
QPDCHAPTASWTSQDTAGCSQWLKALLKVPLAPPVWTAPTFTHRQMLGSTELRREEEEGQRLGVLRKAMA
RGQLLLQRDLGSLPAAEPPPAPESGLEDKLSERLGEAWAGRGAAWWERQQGRTSEPGRQTRRPKRRTQLS
SSFSLSGHVDPSEDTSSPHSPEWPPADALPLPPTTPPSQELTPDACAQGVPSEQRQMLRDYMAKLPPQRD
TPGCATTPPHSQASSVRATRSQQHTPVLSSSQPLRKKPRMGF

TRTRPLE - GFP Tag - V
Restriction Sites SgfI-MluI      Cloning Scheme for this gene      Plasmid Map     
ACCN NM_001243160
ORF Size 1176 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq NM_001243160.2
RefSeq Size 3696 bp
RefSeq ORF 1179 bp
Locus ID 9013
Protein Families Transcription Factors
Gene Summary Initiation of transcription by RNA polymerase I requires the formation of a complex composed of the TATA-binding protein (TBP) and three TBP-associated factors (TAFs) specific for RNA polymerase I. This complex, known as SL1, binds to the core promoter of ribosomal RNA genes to position the polymerase properly and acts as a channel for regulatory signals. This gene encodes the largest SL1-specific TAF. Multiple alternatively spliced transcript variants encoding different isoforms have been identified. [provided by RefSeq, Jul 2011]
*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

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