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SESN1 (NM_001199934) Human Tagged ORF Clone
CAT＃: RG232701
- TrueORF®
SESN1 (tGFP-tagged) - Homo sapiens sestrin 1 (SESN1), transcript variant 3
ORF Plasmid: DDK
"NM_001199934" in other vectors (2)
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CNY 4,370.00
Product images
Specifications
| Product Data | |
| Type | Human Tagged ORF Clone |
| Tag | TurboGFP |
| Synonyms | PA26; SEST1 |
| Vector | pCMV6-AC-GFP |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>RG232701 representing NM_001199934
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGCATGCTTTATTTGCAGATTCTTTTGCTGCTTTGGGCCGTTTGGATAACATTACGTTAGTGATGGTTT TCCACCCACAATATTTAGAAAGTTTCTTAAAAACTCAGCACTATCTACTGCAAATGGATGGGCCGTTACC CCTACATTATCGTCACTACATTGGAATAATGGCTGCGGCAAGACATCAGTGCTCCTACTTAGTGAACCTG CATGTAAATGATTTCCTTCATGTTGGTGGGGACCCCAAGTGGCTCAATGGTTTAGAGAATGCTCCTCAAA AACTACAGAATTTAGGAGAACTTAACAAAGTGTTAGCCCATAGACCTTGGCTTATTACCAAAGAACACAT TGAGGGACTTTTAAAAGCTGAAGAGCACAGCTGGTCCCTTGCGGAATTGGTACATGCAGTAGTTTTACTC ACACACTATCATTCTCTTGCCTCATTCACATTCGGCTGTGGAATCAGTCCAGAAATTCATTGTGATGGTG GCCACACATTCAGACCTCCTTCTGTTAGCAACTACTGCATCTGTGACATTACAAATGGCAATCACAGTGT GGATGAGATGCCGGTCAACTCAGCAGAAAATGTTTCTGTAAGTGATTCTTTCTTTGAGGTTGAAGCCCTC ATGGAAAAGATGAGGCAGTTACAGGAATGTCGAGATGAAGAAGAGGCAAGTCAGGAAGAGATGGCTTCAC GTTTTGAAATAGAAAAAAGAGAGAGTATGTTTGTCTTCTCTTCAGATGATGAAGAAGTTACACCAGCAAG AGCTGTATCTCGTCATTTTGAGGATACTAGTTATGGCTATAAAGATTTCTCTAGACATGGGATGCATGTT CCAACATTTCGTGTCCAGGACTATTGCTGGGAAGATCATGGTTATTCTTTGGTAAATCGCCTTTATCCAG ATGTGGGACAGTTGATTGATGAAAAATTTCACATTGCTTACAATCTTACTTATAATACAATGGCAATGCA CAAAGATGTTGATACCTCAATGCTTAGACGGGCAATTTGGAACTATATTCACTGCATGTTTGGAATAAGA TATGATGATTATGACTATGGTGAAATTAACCAGCTATTGGATCGTAGCTTTAAAGTTTATATCAAAACTG TTGTTTGCACTCCTGAAAAGGTTACCAAAAGAATGTATGATAGCTTCTGGAGGCAGTTCAAGCACTCTGA GAAGGTTCATGTTAATCTGCTTCTTATAGAAGCTAGGATGCAAGCAGAACTCCTTTATGCTCTGAGAGCC ATTACCCGCTATATGACC ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA >RG232701 representing NM_001199934
Red=Cloning site Green=Tags(s) MHALFADSFAALGRLDNITLVMVFHPQYLESFLKTQHYLLQMDGPLPLHYRHYIGIMAAARHQCSYLVNL HVNDFLHVGGDPKWLNGLENAPQKLQNLGELNKVLAHRPWLITKEHIEGLLKAEEHSWSLAELVHAVVLL THYHSLASFTFGCGISPEIHCDGGHTFRPPSVSNYCICDITNGNHSVDEMPVNSAENVSVSDSFFEVEAL MEKMRQLQECRDEEEASQEEMASRFEIEKRESMFVFSSDDEEVTPARAVSRHFEDTSYGYKDFSRHGMHV PTFRVQDYCWEDHGYSLVNRLYPDVGQLIDEKFHIAYNLTYNTMAMHKDVDTSMLRRAIWNYIHCMFGIR YDDYDYGEINQLLDRSFKVYIKTVVCTPEKVTKRMYDSFWRQFKHSEKVHVNLLLIEARMQAELLYALRA ITRYMT TRTRPLE - GFP Tag - V |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_001199934 |
| ORF Size | 1278 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_001199934.1, NP_001186863.1 |
| RefSeq Size | 2740 bp |
| RefSeq ORF | 1281 bp |
| Locus ID | 27244 |
| UniProt ID | Q9Y6P5 |
| Protein Pathways | p53 signaling pathway |
| Gene Summary | This gene encodes a member of the sestrin family. Sestrins are induced by the p53 tumor suppressor protein and play a role in the cellular response to DNA damage and oxidative stress. The encoded protein mediates p53 inhibition of cell growth by activating AMP-activated protein kinase, which results in the inhibition of the mammalian target of rapamycin protein. The encoded protein also plays a critical role in antioxidant defense by regenerating overoxidized peroxiredoxins, and the expression of this gene is a potential marker for exposure to radiation. Alternatively spliced transcript variants encoding multiple isoforms have been observed for this gene. [provided by RefSeq, Dec 2010] |
Documents
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