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EPLIN (LIMA1) (NM_001243775) Human Tagged ORF Clone
CAT＃: RG232779
- TrueORF®
LIMA1 (tGFP-tagged) - Homo sapiens LIM domain and actin binding 1 (LIMA1), transcript variant 4
ORF Plasmid: DDK
"NM_001243775" in other vectors (2)
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CNY 5,040.00
Product images
CNY 1,999.00
CNY 2,700.00
Specifications
| Product Data | |
| Type | Human Tagged ORF Clone |
| Tag | TurboGFP |
| Synonyms | EPLIN; LDLCQ8; SREBP3 |
| Vector | pCMV6-AC-GFP |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>RG232779 representing NM_001243775
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGAGCAAAAGGAGAATGTGCCCCCAGGTCCTGAGGTCTGCATCACCCATCAGGAAGGGGAAAAGATTT CTGCAAATGAGAATAGCCTGGCAGTCCGTTCCACCCCTGCCGAAGATGACTCCCGTGACTCCCAGGTTAA GAGTGAGGTTCAACAGCCTGTCCATCCCAAGCCACTAAGTCCAGATTCCAGAGCCTCCAGTCTTTCTGAA AGTTCTCCTCCCAAAGCAATGAAGAAGTTTCAGGCACCTGCAAGAGAGACCTGCGTGGAATGTCAGAAGA CAGTCTATCCAATGGAGCGTCTCTTGGCCAACCAGCAGGTGTTTCACATCAGCTGCTTCCGTTGCTCCTA TTGCAACAACAAACTCAGTCTAGGAACATATGCATCTTTACATGGAAGAATCTATTGTAAGCCTCACTTC AATCAACTCTTTAAATCTAAGGGCAACTATGATGAAGGCTTTGGGCACAGACCACACAAGGATCTATGGG CAAGCAAAAATGAAAACGAAGAGATTTTGGAGAGACCAGCCCAGCTTGCAAATGCAAGGGAGACCCCTCA CAGCCCAGGGGTAGAAGATGCCCCTATTGCTAAGGTGGGTGTCCTGGCTGCAAGTATGGAAGCCAAGGCC TCCTCTCAGCAGGAGAAGGAAGACAAGCCAGCTGAAACCAAGAAGCTGAGGATCGCCTGGCCACCCCCCA CTGAACTTGGAAGTTCAGGAAGTGCCTTGGAGGAAGGGATCAAAATGTCAAAGCCCAAATGGCCTCCTGA AGACGAAATCAGCAAGCCCGAAGTTCCTGAGGATGTCGATCTAGATCTGAAGAAGCTAAGACGATCTTCT TCACTGAAGGAAAGAAGCCGCCCATTCACTGTAGCAGCTTCATTTCAAAGCACCTCTGTCAAGAGCCCAA AAACTGTGTCCCCACCTATCAGGAAAGGCTGGAGCATGTCAGAGCAGAGTGAAGAGTCTGTGGGTGGAAG AGTTGCAGAAAGGAAACAAGTGGAAAATGCCAAGGCTTCTAAGAAGAATGGGAATGTGGGAAAAACAACC TGGCAAAACAAAGAATCTAAAGGAGAGACAGGGAAGAGAAGTAAGGAAGGTCATAGTTTGGAGATGGAGA ATGAGAATCTTGTAGAAAATGGTGCAGACTCCGATGAAGATGATAACAGCTTCCTCAAACAACAATCTCC ACAAGAACCCAAGTCTCTGAATTGGTCGAGTTTTGTAGACAACACCTTTGCTGAAGAATTCACTACTCAG AATCAGAAATCCCAGGATGTGGAACTCTGGGAGGGAGAAGTGGTCAAAGAGCTCTCTGTGGAAGAACAGA TAAAGAGAAATCGGTATTATGATGAGGATGAGGATGAAGAG ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA >RG232779 representing NM_001243775
Red=Cloning site Green=Tags(s) MEQKENVPPGPEVCITHQEGEKISANENSLAVRSTPAEDDSRDSQVKSEVQQPVHPKPLSPDSRASSLSE SSPPKAMKKFQAPARETCVECQKTVYPMERLLANQQVFHISCFRCSYCNNKLSLGTYASLHGRIYCKPHF NQLFKSKGNYDEGFGHRPHKDLWASKNENEEILERPAQLANARETPHSPGVEDAPIAKVGVLAASMEAKA SSQQEKEDKPAETKKLRIAWPPPTELGSSGSALEEGIKMSKPKWPPEDEISKPEVPEDVDLDLKKLRRSS SLKERSRPFTVAASFQSTSVKSPKTVSPPIRKGWSMSEQSEESVGGRVAERKQVENAKASKKNGNVGKTT WQNKESKGETGKRSKEGHSLEMENENLVENGADSDEDDNSFLKQQSPQEPKSLNWSSFVDNTFAEEFTTQ NQKSQDVELWEGEVVKELSVEEQIKRNRYYDEDEDEE TRTRPLE - GFP Tag - V |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_001243775 |
| ORF Size | 1371 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_001243775.1, NP_001230704.1 |
| RefSeq Size | 3356 bp |
| RefSeq ORF | 1374 bp |
| Locus ID | 51474 |
| UniProt ID | Q9UHB6 |
| Gene Summary | This gene encodes a cytoskeleton-associated protein that inhibits actin filament depolymerization and cross-links filaments in bundles. It is downregulated in some cancer cell lines. Alternatively spliced transcript variants encoding different isoforms have been described for this gene, and expression of some of the variants maybe independently regulated. [provided by RefSeq, Aug 2011] |
Documents
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