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DEPDC5 (NM_001242896) Human Tagged ORF Clone
CAT＃: RG233214
- TrueORF®
DEPDC5 (tGFP-tagged) - Homo sapiens DEP domain containing 5 (DEPDC5), transcript variant 4
ORF Plasmid: DDK
"NM_001242896" in other vectors (2)
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CNY 17,580.00
货期*
7周
规格
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经常一起买 (4)
Specifications
| Product Data | |
| Type | Human Tagged ORF Clone |
| Tag | TurboGFP |
| Synonyms | DEP.5; FFEVF; FFEVF1 |
| Vector | pCMV6-AC-GFP |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>RG233214 representing NM_001242896
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGAGAACAACAAAGGTCTACAAACTCGTCATCCACAAGAAGGGCTTTGGGGGCAGTGATGATGAGCTAG TTGTGAACCCCAAAGTGTTCCCTCACATCAAGCTTGGAGACATTGTAGAGATTGCACACCCCAACGATGA ATACAGCCCTCTGCTTTTGCAGGTCAAGTCTCTTAAGGAAGATTTACAGAAGGAAACTATCAGTGTGGAC CAGACTGTGACTCAAGTGTTCCGGCTGAGACCTTATCAGGATGTCTATGTTAATGTCGTAGACCCTAAGG ATGTGACCCTTGACCTAGTGGAATTAACTTTTAAGGATCAGTATATTGGCCGTGGGGATATGTGGCGACT AAAGAAAAGTTTGGTCAGCACATGTGCCTATATCACCCAGAAGGTGGAGTTTGCTGGCATCAGAGCACAG GCTGGTGAACTGTGGGTTAAGAATGAGAAGGTCATGTGTGGCTACATCAGTGAAGATACCAGGGTGGTGT TTCGTTCTACGTCGGCTATGGTTTACATATTTATTCAGATGAGCTGTGAAATGTGGGATTTTGATATTTA TGGGGATTTGTATTTTGAGAAAGCTGTGAATGGTTTCCTTGCTGATCTATTTACCAAGTGGAAGGAGAAG AACTGTAGTCATGAAGTGACAGTGGTCCTGTTTTCTAGAACTTTCTATGATGCAAAATCTGTTGATGAAT TTCCTGAAATAAACCGAGCCTCAATTCGACAGGATCACAAGGGGAGATTCTATGAAGACTTTTACAAAGT GGTGGTGCAGAATGAGAGAAGAGAAGAATGGACTTCACTTCTCGTAACCATTAAAAAACTCTTCATCCAG TATCCAGTGTTGGTGCGACTGGAACAGGCAGAGGGCTTTCCTCAAGGAGATAATTCTACCTCAGCACAAG GAAACTACCTGGAGGCCATCAATCTGTCATTCAATGTGTTTGATAAGCACTACATCAACCGCAACTTTGA CCGAACTGGGCAGATGTCAGTGGTGATCACGCCCGGGGTGGGTGTCTTTGAAGTGGACCGCCTACTCATG ATCCTGACCAAGCAGCGGATGATAGATAATGGAATTGGTGTGGATTTGGTGTGCATGGGAGAGCAACCGT TACATGCTGTCCCATTGTTCAAGCTCCATAATCGGAGTGCTCCCCGTGATTCTCGTCTGGGCGATGACTA TAATATCCCTCACTGGATAAACCACAGTTTCTACACATCCAAAAGCCAGCTCTTTTGTAATAGTTTCACC CCACGAATAAAACTGGCAGGAAAGAAGCCCGCCTCTGAGAAAGCAAAAAATGGCCGTGATACATCTCTCG GGAGTCCAAAAGAATCTGAGAACGCCCTTCCCATCCAAGTAGATTATGACGCCTATGACGCTCAAGTGTT CAGGCTGCCCGGCCCATCCCGGGCCCAGTGCCTCACCACCTGCAGATCTGTGCGAGAGCGAGAGAGTCAC AGTCGAAAGAGTGCCAGCTCCTGTGATGTTTCATCCAGCCCTTCCCTACCAAGCCGCACACTGCCCACTG AGGAAGTGAGGAGCCAGGCTTCTGACGACAGCTCCCTAGGCAAGAGTGCCAACATCCTGATGATCCCACA CCCCCACCTGCACCAGTATGAAGTCAGCAGCTCCTTGGGATACACCAGCACTCGAGATGTCCTGGAGAAC ATGATGGAGCCACCACAGCGAGACTCCAGTGCACCAGGGAGGTTTCACGTTGGCAGTGCAGAATCCATGC TGCATGTTCGACCTGGTGGATACACGCCCCAGAGAGCACTGATTAACCCCTTCGCTCCCTCTCGGATGCC CATGAAGCTTACGTCCAACAGAAGGCGCTGGATGCACACTTTTCCTGTGGGGCCATCCGGAGAAGCCATC CAGATCCACCACCAGACCCGACAGAATATGGCGGAGCTACAAGGCAGCGGGCAGAGGGATCCAACTCACT CCTCTGCAGAGCTGCTGGAGTTAGCATATCATGAAGCTGCTGGAAGGCACAGCAATTCCCGCCAGCCTGG TGACGGCATGTCCTTCTTGAACTTCAGTGGAACAGAGGAGCTTTCTGTCGGCCTGCTTAGCAACAGTGGT GCAGGTATGAATCCTAGGACCCAGAATAAGGATTCTCTAGAGGACAGTGTTTCTACCTCTCCAGACCCAA TTCTGACACTGTCTGCTCCCCCTGTAGTGCCAGGCTTCTGTTGCACAGTTGGAGTGGACTGGAAGTCTCT CACTACTCCGGCGTGCCTCCCCCTTACCACCGACTACTTCCCTGACCGCCAGGGCCTGCAGAATGACTAC ACAGAGGGCTGTTATGATCTCCTTCCAGAAGCAGACATCGACAGGAGGGACGAAGATGGTGTGCAGATGA CAGCCCAGCAGGTATTTGAAGAGTTTATTTGCCAACGTCTCATGCAGGGCTACCAAATCATAGTGCAGCC CAAGACACAGAAACCCAATCCTGCTGTCCCGCCCCCGCTGAGCAGTAGCCCACTCTATAGCCGAGGCCTT GTGTCCCGAAACCGCCCTGAGGAGGAGGACCAGTATTGGCTGAGTATGGGCAGAACGTTCCACAAAGTGA CGCTGAAGGATAAGATGATCACAGTGACGCGATACCTTCCCAAGTATCCTTATGAATCTGCCCAGATCCA CTACACCTACAGCCTCTGTCCTTCCCACTCAGACTCAGAGTTCGTCTCCTGCTGGGTGGAATTCTCCCAC GAACGGCTGGAGGAGTACAAGTGGAATTACTTAGATCAGTATATCTGTTCTGCCGGCTCTGAAGACTTCA GCTTAATTGAGTCCCTGAAGTTCTGGAGGACCCGCTTCCTGCTGCTGCCAGCCTGTGTCACCGCCACCAA GCGCATCACGGAGGGGGAGGCCCACTGCGACATCTATGGGGACAGGCCCCGTGCAGACGAGGACGAGTGG CAACTCCTGGATGGTTTTGTCCGCTTTGTGGAGGGCTTGAATCGCATTCGCAGGCGGCATCGCTCGGATC GCATGATGCGGAAAGGGACCGCCATGAAAGGCTTGCAGATGACTGGGCCCATTTCCACGCATTCTCTGGA GTCAACTGCACCCCCAGTGGGGAAGAAGGGAACCTCAGCTCTCTCTGCCCTGTTGGAGATGGAGGCCAGT CAGAAGTGCCTGGGAGAACAGCAGGCAGCTGTGCATGGTGGGAAGAGCTCCGCCCAGTCAGCCGAGAGCA GCAGCGTTGCCATGACTCCCACCTACATGGACAGCCCACGAAAGGACGGGGCCTTCTTTATGGAGTTTGT CCGCAGCCCACGCACAGCATCGTCCGCCTTCTACCCTCAGGTATCTGTGGACCAAACAGCCACTCCTATG TTGGACGGCACCAGTTTGGGCATATGCACAGGCCAATCCATGGACAGAGGCAACAGCCAGACCTTTGGGA ACTCCCAGAACATAGGAGAACAGGGCTACTCCTCCACAAACTCCAGTGACAGCAGCTCTCAGCAGCTGGT GGCAAGCTCCTTGACCTCATCCTCTACCCTGACAGAGATCCTGGAAGCCATGAAGCACCCCTCGACAGGA GTCCAGCTGCTCTCTGAACAGAAGGGCCTCTCACCGTACTGCTTCATCAGCGCGGAGGTGGTACACTGGT TGGTGAACCACGTGGAGGGGATCCAGACACAGGCGATGGCCATTGACATCATGCAGAAAATGCTGGAAGA GCAGCTCATCACACATGCATCTGGCGAAGCCTGGCGGACCTTCATCTACGGCTTCTATTTCTACAAGATA GTAACGGACAAAGAGCCCGACCGAGTGGCCATGCAGCAGCCCGCCACCACCTGGCACACAGCAGGAGTGG ACGACTTCGCCAGCTTCCAGCGCAAGTGGTTTGAGGTGGCCTTTGTGGCAGAAGAGCTCGTGCACTCTGA GATTCCTGCCTTTCTCCTGCCCTGGCTGCCTAGCCGGCCAGCCTCCTATGCAAGTAGGCACAGCTCCTTT AGCCGAAGTTTTGGAGGACGGAGCCAGGCGGCAGCACTTTTAGCTGCCACTGTCCCAGAGCAGAGGACTG TGACCCTGGATGTTGACGTGAACAACCGCACAGACCGGCTGGAGTGGTGCAGCTGTTATTACCATGGCAA CTTTTCTCTGAATGCAGCCTTTGAGATCAAGCTGCACTGGATGGCGGTGACCGCAGCAGTACTCTTCGAG ATGGTCCAAGGTTGGCATCGGAAAGCCACCTCCTGTGGCTTCTTGTTAGTCCCAGTTTTGGAGGGGCCTT TTGCACTGCCCAGTTACCTGTATGGCGACCCCCTTCGTGCCCAGCTCTTCATCCCACTCAACATCAGCTG CTTGCTCAAGGAGGGCAGCGAGCACCTGTTTGATAGCTTTGAACCCGAAACGTACTGGGATCGAATGCAC CTCTTCCAGGAAGCCATTGCACACAGGTTTGGGTTTGTACAAGATAAATATTCTGCCTCTGCTTTTAACT TCCCTGCTGAGAACAAGCCTCAGTATATCCACGTTACAGGAACAGTGTTTCTGCAGCTGCCCTACTCCAA GCGCAAGTTCTCAGGGCAGCAGCGGCGGCGGCGGAACTCCACCAGCTCCACCAACCAGAACATGTTCTGC GAGGAGCGGGTCGGCTACAACTGGGCCTACAACACCATGCTCACCAAAACATGGCGCTCCAGCGCCACAG GGGATGAAAAGTTTGCTGATCGGCTGCTGAAGGACTTCACGGACTTCTGCATCAACCGTGACAACCGGCT GGTCACGTTCTGGACAAGTTGCCTGGAGAAGATGCATGCCAGTGCCCCG ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA >RG233214 representing NM_001242896
Red=Cloning site Green=Tags(s) MRTTKVYKLVIHKKGFGGSDDELVVNPKVFPHIKLGDIVEIAHPNDEYSPLLLQVKSLKEDLQKETISVD QTVTQVFRLRPYQDVYVNVVDPKDVTLDLVELTFKDQYIGRGDMWRLKKSLVSTCAYITQKVEFAGIRAQ AGELWVKNEKVMCGYISEDTRVVFRSTSAMVYIFIQMSCEMWDFDIYGDLYFEKAVNGFLADLFTKWKEK NCSHEVTVVLFSRTFYDAKSVDEFPEINRASIRQDHKGRFYEDFYKVVVQNERREEWTSLLVTIKKLFIQ YPVLVRLEQAEGFPQGDNSTSAQGNYLEAINLSFNVFDKHYINRNFDRTGQMSVVITPGVGVFEVDRLLM ILTKQRMIDNGIGVDLVCMGEQPLHAVPLFKLHNRSAPRDSRLGDDYNIPHWINHSFYTSKSQLFCNSFT PRIKLAGKKPASEKAKNGRDTSLGSPKESENALPIQVDYDAYDAQVFRLPGPSRAQCLTTCRSVRERESH SRKSASSCDVSSSPSLPSRTLPTEEVRSQASDDSSLGKSANILMIPHPHLHQYEVSSSLGYTSTRDVLEN MMEPPQRDSSAPGRFHVGSAESMLHVRPGGYTPQRALINPFAPSRMPMKLTSNRRRWMHTFPVGPSGEAI QIHHQTRQNMAELQGSGQRDPTHSSAELLELAYHEAAGRHSNSRQPGDGMSFLNFSGTEELSVGLLSNSG AGMNPRTQNKDSLEDSVSTSPDPILTLSAPPVVPGFCCTVGVDWKSLTTPACLPLTTDYFPDRQGLQNDY TEGCYDLLPEADIDRRDEDGVQMTAQQVFEEFICQRLMQGYQIIVQPKTQKPNPAVPPPLSSSPLYSRGL VSRNRPEEEDQYWLSMGRTFHKVTLKDKMITVTRYLPKYPYESAQIHYTYSLCPSHSDSEFVSCWVEFSH ERLEEYKWNYLDQYICSAGSEDFSLIESLKFWRTRFLLLPACVTATKRITEGEAHCDIYGDRPRADEDEW QLLDGFVRFVEGLNRIRRRHRSDRMMRKGTAMKGLQMTGPISTHSLESTAPPVGKKGTSALSALLEMEAS QKCLGEQQAAVHGGKSSAQSAESSSVAMTPTYMDSPRKDGAFFMEFVRSPRTASSAFYPQVSVDQTATPM LDGTSLGICTGQSMDRGNSQTFGNSQNIGEQGYSSTNSSDSSSQQLVASSLTSSSTLTEILEAMKHPSTG VQLLSEQKGLSPYCFISAEVVHWLVNHVEGIQTQAMAIDIMQKMLEEQLITHASGEAWRTFIYGFYFYKI VTDKEPDRVAMQQPATTWHTAGVDDFASFQRKWFEVAFVAEELVHSEIPAFLLPWLPSRPASYASRHSSF SRSFGGRSQAAALLAATVPEQRTVTLDVDVNNRTDRLEWCSCYYHGNFSLNAAFEIKLHWMAVTAAVLFE MVQGWHRKATSCGFLLVPVLEGPFALPSYLYGDPLRAQLFIPLNISCLLKEGSEHLFDSFEPETYWDRMH LFQEAIAHRFGFVQDKYSASAFNFPAENKPQYIHVTGTVFLQLPYSKRKFSGQQRRRRNSTSSTNQNMFC EERVGYNWAYNTMLTKTWRSSATGDEKFADRLLKDFTDFCINRDNRLVTFWTSCLEKMHASAP TRTRPLE - GFP Tag - V |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_001242896 |
| ORF Size | 4809 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_001242896.3 |
| RefSeq Size | 5551 bp |
| RefSeq ORF | 4812 bp |
| Locus ID | 9681 |
| UniProt ID | O75140 |
| Gene Summary | This gene encodes a member of the IML1 family of proteins involved in G-protein signaling pathways. The mechanistic target of rapamycin complex 1 (mTORC1) pathway regulates cell growth by sensing the availability of nutrients. The protein encoded by this gene is a component of the GATOR1 (GAP activity toward Rags) complex which inhibits the amino acid-sensing branch of the mTORC1 pathway. Mutations in this gene are associated with autosomal dominant familial focal epilepsy with variable foci. A single nucleotide polymorphism in an intron of this gene has been associated with an increased risk of hepatocellular carcinoma in individuals with chronic hepatitis C virus infection. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Mar 2014] |
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