Bcl rambo (BCL2L13) (NM_001270730) Human Tagged ORF Clone
CAT#: RG233220
- TrueORF®
BCL2L13 (tGFP-tagged) - Homo sapiens BCL2-like 13 (apoptosis facilitator) (BCL2L13), transcript variant 6
ORF Plasmid: DDK
"NM_001270730" in other vectors (2)
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CNY 4,370.00
Specifications
Product Data | |
Type | Human Tagged ORF Clone |
Tag | TurboGFP |
Synonyms | BCL-RAMBO; Bcl2-L-13; MIL1 |
Vector | pCMV6-AC-GFP |
E. coli Selection | Ampicillin (100 ug/mL) |
Mammalian Cell Selection | Neomycin |
Sequence Data |
>RG233220 representing NM_001270730
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGCTTTTGGAATTGACAAGACGTGGTCAAGAACCTTTGAGCGCACTGCTGCAGTTTGGCGTGACATACC TGGAGGACTATTCGGCAGAGTACATCATTCAGCAAGGTGGCTGGGGCACTGTGTTTAGTCTTGAGTCAGA GGAGGAGGAATACCCTGGAATCACTGCAGAAGATAGCAATGACATTTACATCCTGCCCAGCGACAACTCT GGACAAGTCAGTCCCCCAGAGTCTCCAACTGTGACCACTTCCTGGCAGTCTGAGAGCTTACCTGTGTCAC TGTCAGCTAGCCAGAGTTGGCACACAGAAAGCCTGCCAGTGTCACTAGGCCCTGAGTCCTGGCAGCAGAT TGCAATGGATCCTGAAGAAGTGAAAAGCTTAGACAGCAACGGAGCTGGAGAGAAGAGTGAGAACAACTCC TCTAATTCTGACATTGTGCACGTGGAGAAAGAAGAGGTGCCCGAGGGCATGGAAGAGGCTGCTGTGGCTT CTGTGGTCTTGCCAGCGCGGGAGCTGCAAGAGGCACTTCCTGAAGCCCCAGCTCCCTTGCTTCCACATAT CACTGCCACCTCCCTGCTGGGGACAAGGGAACCTGACACAGAAGTGATCACAGTTGAGAAATCCAGCCCT GCTACATCTCTGTTTGTAGAACTTGATGAAGAAGAGGTGAAAGCAGCAACAACTGAACCTACTGAAGTGG AGGAGGTGGTCCCCGCACTGGAACCCACAGAAACGCTGCTGAGTGAGAAGGAGATAAACGCAAGGGAAGA GAGCCTTGTGGAAGAGCTGTCCCCTGCCAGCGAGAAGAAGCCCGTGCCGCCGTCTGAGGGCAAGTCTAGA CTGTCCCCCGCCGGTGAGATGAAGCCCATGCCGCTGTCTGAGGGCAAGTCTATACTGCTGTTTGGAGGGG CTGCTGCTGTTGCCATCCTGGCAGTGGCCATCGGGGTAGCCCTGGCTCTGAGAAAGAAA ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA >RG233220 representing NM_001270730
Red=Cloning site Green=Tags(s) MLLELTRRGQEPLSALLQFGVTYLEDYSAEYIIQQGGWGTVFSLESEEEEYPGITAEDSNDIYILPSDNS GQVSPPESPTVTTSWQSESLPVSLSASQSWHTESLPVSLGPESWQQIAMDPEEVKSLDSNGAGEKSENNS SNSDIVHVEKEEVPEGMEEAAVASVVLPARELQEALPEAPAPLLPHITATSLLGTREPDTEVITVEKSSP ATSLFVELDEEEVKAATTEPTEVEEVVPALEPTETLLSEKEINAREESLVEELSPASEKKPVPPSEGKSR LSPAGEMKPMPLSEGKSILLFGGAAAVAILAVAIGVALALRKK TRTRPLE - GFP Tag - V |
Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
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ACCN | NM_001270730 |
ORF Size | 969 bp |
OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
Reference Data | |
RefSeq | NM_001270730.1, NP_001257659.1 |
RefSeq Size | 4804 bp |
RefSeq ORF | 972 bp |
Locus ID | 23786 |
UniProt ID | Q9BXK5 |
Protein Families | Druggable Genome, Transmembrane |
Gene Summary | This gene encodes a mitochondrially-localized protein with conserved B-cell lymphoma 2 homology motifs. Overexpression of the encoded protein results in apoptosis. Alternatively spliced transcript variants have been observed for this gene. [provided by RefSeq, Jul 2012] |
Documents
Product Manuals |
FAQs |
SDS |
Resources
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