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ZNF289 (ARFGAP2) (NM_001242832) Human Tagged ORF Clone
CAT＃: RG234276
- TrueORF®
ARFGAP2 (tGFP-tagged) - Homo sapiens ADP-ribosylation factor GTPase activating protein 2 (ARFGAP2), transcript variant 2
ORF Plasmid: DDK
"NM_001242832" in other vectors (2)
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CNY 4,370.00
Product images
Specifications
| Product Data | |
| Type | Human Tagged ORF Clone |
| Tag | TurboGFP |
| Synonyms | IRZ; NBLA10535; ZFP289; ZNF289 |
| Vector | pCMV6-AC-GFP |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>RG234276 representing NM_001242832
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGCGGCGGAGCCGAACAAGACCGAAATCCAGACTCTTTTTAAGAGGCTTCGCGCAGTTCCAACCAACA AGGCCTGTTTCGACTGCGGCGCCAAGAATCCGAGTTGGGCCAGCATCACGTACGGTGTTTTCTTGTGCAT TGACTGTTCCGGGGTGCACCGCTCCCTGGGCGTCCATCTGAGCTTCATCAGGTCCACAGAGTTGGATTCC AACTGGAACTGGTTCCAGCTGAGGTGTATGCAGGTCGGCGGGAATGCCAATGCGACGGCTTTTTTTCGCC AACATGGATGCACAGCCAATGATGCCAACACCAAATATAATAGCCGAGCTGCCCAGATGTACCGGGAGAA GATCCGGCAGCTGGGGAGTGCGGCCCTGGCTAGGCATGGCACTGATCCCCCTGCCTGGGATGCGCCAGCC ACTGAGCCTTCAGGGACCCAGCAGCCAGCCCCGTCTACAGAGAGCAGTGGCCTGGCACAGCCGGAGCATG GCCCCAACACAGACCTGCTTGGCACCTCACCCAAAGCCTCACTGGAACTGAAAAGCTCCATCATTGGCAA GAAGAAGCCAGCAGCAGCTAAGAAAGGGCTGGGTGCCAAGAAAGGCCTAGGGGCCCAGAAGGTGAGCAGC CAGAGCTTCAGTGAGATTGAGCGGCAGGCTCAGGTGGCAGAGAAGCTCCGTGAGCAGCAGGCAGCCGATG CCAAGAAGCAGGCGGAGGAGTCCATGGTCGCCTCCATGCGTCTGGCCTACCAGGAGCTCCAGATTGATCG TAAGAAAGAGGAAAAGAAGCTACAGAATCTGGAAGGGAAGAAGCGAGAGCAGGCAGAAAGGTTGGGCATG GGCTTGGTATCCCGAAGCTCTGTCTCCCACTCCGTGCTGTCTGAGATGCAGGTGATTGAGCAGGAAACCC CAGTGAGTGCAAAATCCTCTCGCTCGCAGCTGGACTTGTTTGACGATGTTGGTACTTTCGCCTCTGGACC CCCAAAGTACAAGGACAATCCCTTTTCCTTAGGGGAAAGCTTTGGCTCCCGCTGGGATACAGATGCTGCC TGGGGTATGGACAGGGTAGAGGAGAAGGAGCCAGAAGTGACCATCTCAAGCATCCGGCCTATTTCAGAAA GAGCCACAAACCGGAGGGAAGTGGAGAGCCGGAGCTCAGGCCTCGAGTCTAGTGAGGCGCGTCAGAAATT CGCAGGAGCCAAAGCCATCTCATCTGACATGTTCTTTGGGCGGGAGGTGGATGCGGAGTATGAGGCCAGG TCTCGGCTGCAGCAGCTCTCAGGCAGCAGTGCCATCAGCTCTTCAGACCTCTTTGGGGACATGGATGGAG CTCACGGAGCAGGAAGTGTATCTCTGGGGAACGTGCTGCCTACAGCGGACATTGCCCAGTTTAAGCAGGG TGTCAAGTCTGTGGCTGGGAAAATGGCTGTGCTGGCCAATGGTGTGATGAATTCCTTGCAGGATCGCTAC GGTTCCTAC ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA >RG234276 representing NM_001242832
Red=Cloning site Green=Tags(s) MAAEPNKTEIQTLFKRLRAVPTNKACFDCGAKNPSWASITYGVFLCIDCSGVHRSLGVHLSFIRSTELDS NWNWFQLRCMQVGGNANATAFFRQHGCTANDANTKYNSRAAQMYREKIRQLGSAALARHGTDPPAWDAPA TEPSGTQQPAPSTESSGLAQPEHGPNTDLLGTSPKASLELKSSIIGKKKPAAAKKGLGAKKGLGAQKVSS QSFSEIERQAQVAEKLREQQAADAKKQAEESMVASMRLAYQELQIDRKKEEKKLQNLEGKKREQAERLGM GLVSRSSVSHSVLSEMQVIEQETPVSAKSSRSQLDLFDDVGTFASGPPKYKDNPFSLGESFGSRWDTDAA WGMDRVEEKEPEVTISSIRPISERATNRREVESRSSGLESSEARQKFAGAKAISSDMFFGREVDAEYEAR SRLQQLSGSSAISSSDLFGDMDGAHGAGSVSLGNVLPTADIAQFKQGVKSVAGKMAVLANGVMNSLQDRY GSY TRTRPLE - GFP Tag - V |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_001242832 |
| ORF Size | 1479 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_001242832.1, NP_001229761.1 |
| RefSeq Size | 2955 bp |
| RefSeq ORF | 1482 bp |
| Locus ID | 84364 |
| UniProt ID | Q8N6H7 |
| Protein Pathways | Endocytosis |
| Gene Summary | GTPase-activating protein (GAP) for ADP ribosylation factor 1 (ARF1). Implicated in coatomer-mediated protein transport between the Golgi complex and the endoplasmic reticulum. Hydrolysis of ARF1-bound GTP may lead to dissociation of coatomer from Golgi-derived membranes to allow fusion with target membranes.[UniProtKB/Swiss-Prot Function] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC234276 | ARFGAP2 (Myc-DDK tagged) - Homo sapiens ADP-ribosylation factor GTPase activating protein 2 (ARFGAP2), transcript variant 2 |
CNY 3,990.00 |
|
| SC331890 | ARFGAP2 (untagged) - Homo sapiens ADP-ribosylation factor GTPase activating protein 2 (ARFGAP2), transcript variant 2 |
CNY 4,660.00 |
