ZNF289 (ARFGAP2) (NM_001242832) Human Untagged Clone

Specifications

Product Data
Type	Human Untagged Clone
Tag	Tag Free
Synonyms	IRZ; NBLA10535; ZFP289; ZNF289
Vector	pCMV6-Entry
Sequence Data	Fully Sequenced ORF (showhide) >SC331890 representing NM_001242832. Blue=Insert sequence Red=Cloning site Green=Tag(s) ATGGCGGCGGAGCCGAACAAGACCGAAATCCAGACTCTTTTTAAGAGGCTTCGCGCAGTTCCAACCAAC AAGGCCTGTTTCGACTGCGGCGCCAAGAATCCGAGTTGGGCCAGCATCACGTACGGTGTTTTCTTGTGC ATTGACTGTTCCGGGGTGCACCGCTCCCTGGGCGTCCATCTGAGCTTCATCAGGTCCACAGAGTTGGAT TCCAACTGGAACTGGTTCCAGCTGAGGTGTATGCAGGTCGGCGGGAATGCCAATGCGACGGCTTTTTTT CGCCAACATGGATGCACAGCCAATGATGCCAACACCAAATATAATAGCCGAGCTGCCCAGATGTACCGG GAGAAGATCCGGCAGCTGGGGAGTGCGGCCCTGGCTAGGCATGGCACTGATCCCCCTGCCTGGGATGCG CCAGCCACTGAGCCTTCAGGGACCCAGCAGCCAGCCCCGTCTACAGAGAGCAGTGGCCTGGCACAGCCG GAGCATGGCCCCAACACAGACCTGCTTGGCACCTCACCCAAAGCCTCACTGGAACTGAAAAGCTCCATC ATTGGCAAGAAGAAGCCAGCAGCAGCTAAGAAAGGGCTGGGTGCCAAGAAAGGCCTAGGGGCCCAGAAG GTGAGCAGCCAGAGCTTCAGTGAGATTGAGCGGCAGGCTCAGGTGGCAGAGAAGCTCCGTGAGCAGCAG GCAGCCGATGCCAAGAAGCAGGCGGAGGAGTCCATGGTCGCCTCCATGCGTCTGGCCTACCAGGAGCTC CAGATTGATCGTAAGAAAGAGGAAAAGAAGCTACAGAATCTGGAAGGGAAGAAGCGAGAGCAGGCAGAA AGGTTGGGCATGGGCTTGGTATCCCGAAGCTCTGTCTCCCACTCCGTGCTGTCTGAGATGCAGGTGATT GAGCAGGAAACCCCAGTGAGTGCAAAATCCTCTCGCTCGCAGCTGGACTTGTTTGACGATGTTGGTACT TTCGCCTCTGGACCCCCAAAGTACAAGGACAATCCCTTTTCCTTAGGGGAAAGCTTTGGCTCCCGCTGG GATACAGATGCTGCCTGGGGTATGGACAGGGTAGAGGAGAAGGAGCCAGAAGTGACCATCTCAAGCATC CGGCCTATTTCAGAAAGAGCCACAAACCGGAGGGAAGTGGAGAGCCGGAGCTCAGGCCTCGAGTCTAGT GAGGCGCGTCAGAAATTCGCAGGAGCCAAAGCCATCTCATCTGACATGTTCTTTGGGCGGGAGGTGGAT GCGGAGTATGAGGCCAGGTCTCGGCTGCAGCAGCTCTCAGGCAGCAGTGCCATCAGCTCTTCAGACCTC TTTGGGGACATGGATGGAGCTCACGGAGCAGGAAGTGTATCTCTGGGGAACGTGCTGCCTACAGCGGAC ATTGCCCAGTTTAAGCAGGGTGTCAAGTCTGTGGCTGGGAAAATGGCTGTGCTGGCCAATGGTGTGATG AATTCCTTGCAGGATCGCTACGGTTCCTACTGA
Restriction Sites	SgfI-MluI
ACCN	NM_001242832
Insert Size	1482 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq	NM_001242832.1
RefSeq Size	2955 bp
RefSeq ORF	1482 bp
Locus ID	84364
UniProt ID	Q8N6H7
Protein Pathways	Endocytosis
MW	53.5 kDa
Gene Summary	GTPase-activating protein (GAP) for ADP ribosylation factor 1 (ARF1). Implicated in coatomer-mediated protein transport between the Golgi complex and the endoplasmic reticulum. Hydrolysis of ARF1-bound GTP may lead to dissociation of coatomer from Golgi-derived membranes to allow fusion with target membranes.[UniProtKB/Swiss-Prot Function] Transcript Variant: This variant (2) lacks an alternate in-frame exon in the 5' coding region, compared to variant 1, resulting in an isoform (2) that is shorter than isoform 1.

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