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TCP1 delta (CCT4) (NM_001256721) Human Tagged ORF Clone
CAT＃: RG234317
- TrueORF®
CCT4 (tGFP-tagged) - Homo sapiens chaperonin containing TCP1, subunit 4 (delta) (CCT4), transcript variant 2
ORF Plasmid: DDK
"NM_001256721" in other vectors (2)
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CNY 5,040.00
Product images
CNY 1,999.00
CNY 2,700.00
Specifications
| Product Data | |
| Type | Human Tagged ORF Clone |
| Tag | TurboGFP |
| Synonyms | CCT-DELTA; Cctd; SRB |
| Vector | pCMV6-AC-GFP |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>RG234317 representing NM_001256721
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGCCCGAGAATGTGGCACCCCGGAGCGGGGCGACTGCCGGGGCTGCCGGCGGCCGCGGGAAAGGCGCCT ATCAGGACCGCGACAAGCCAGCCCAGATCCGCTTCAGCAACATTTCCGCCGCCAAAGCGGTTGCTGATGC TATTAGAACAAGCCTTGGACCAAAAGGAATGGATAAAATGCTGGTGGAGCTGTCTAAGGCTCAAGATATA GAAGCAGGAGATGGCACCACATCAGTAGTCATCATTGCTGGCTCCCTCTTAGATTCTTGTACCAAGCTTC TTCAGAAAGGGATTCATCCAACCATCATTTCTGAGTCATTCCAGAAGGCCCTGGAAAAGGGCATTGAAAT CTTGACTGACATGTCTCGACCTGTGGAACTGAGTGACAGAGAAACTTTGTTAAATAGTGCAACCACTTCA CTGAACTCAAAGGTGGTTTCTCAGTATTCAAGTCTGCTTTCTCCAATGAGTGTAAATGCAGTGATGAAAG TGATTGACCCAGCCACAGCCACCAGTGTAGATCTTAGAGATATTAAAATAGTTAAGAAGCTTGGTGGGAC AATTGATGACTGTGAGTTGGTGGAAGGGCTGGTTCTCACCCAAAAAGTGTCAAATTCTGGCATAACCAGA GTTGAAAAGGCCAAGATTGGGCTTATTCAGTTTTGCTTATCTGCTCCCAAAACAGACATGGATAATCAAA TAGTGGTTTCTGACTATGCCCAGATGGACCGAGTGCTGCGAGAAGAGAGAGCCTATATTTTAAATTTAGT GAAGCAAATTAAAAAAACAGGATGTAATGTCCTTCTCATACAGAAATCTATTCTAAGAGATGCTCTTAGT GATCTTGCATTACACTTTCTGAATAAAATGAAGATCATGGTGATTAAGGATATTGAAAGAGAAGACATTG AATTCATTTGTAAGACAATTGGAACCAAGCCAGTTGCTCATATTGACCAATTTACTGCTGACATGCTGGG TTCTGCTGAGTTAGCTGAGGAGGTCAATTTAAATGGTTCTGGCAAACTGCTCAAGATTACAGGCTGTGCC AGCCCTGGAAAAACAGTTACAATTGTTGTTCGTGGTTCTAACAAACTGGTGATTGAAGAAGCTGAGCGCT CCATTCATGATGCCCTATGTGTTATTCGTTGTTTAGTGAAGAAGAGGGCTCTTATTGCAGGAGGTGGTGC TCCAGAAATAGAGTTGGCCCTACGATTAACTGAATATTCACGAACACTGAGTGGTATGGAATCCTACTGC GTTCGTGCTTTTGCAGATGCTATGGAGGTCATTCCATCTACACTAGCTGAAAATGCCGGCCTGAATCCCA TTTCTACAGTAACAGAACTAAGAAACCGGCATGCCCAGGGAGAAAAAACTGCAGGCATTAATGTCCGAAA GGGTGGTATTTCCAACATTTTGGAGGAACTGGTTGTCCAGCCTCTGTTGGTATCAGTCAGTGCTCTGACT CTTGCAACTGAAACTGTTCGGAGCATTCTGAAAATAGATGATGTGGTAAACACTCGA ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA >RG234317 representing NM_001256721
Red=Cloning site Green=Tags(s) MPENVAPRSGATAGAAGGRGKGAYQDRDKPAQIRFSNISAAKAVADAIRTSLGPKGMDKMLVELSKAQDI EAGDGTTSVVIIAGSLLDSCTKLLQKGIHPTIISESFQKALEKGIEILTDMSRPVELSDRETLLNSATTS LNSKVVSQYSSLLSPMSVNAVMKVIDPATATSVDLRDIKIVKKLGGTIDDCELVEGLVLTQKVSNSGITR VEKAKIGLIQFCLSAPKTDMDNQIVVSDYAQMDRVLREERAYILNLVKQIKKTGCNVLLIQKSILRDALS DLALHFLNKMKIMVIKDIEREDIEFICKTIGTKPVAHIDQFTADMLGSAELAEEVNLNGSGKLLKITGCA SPGKTVTIVVRGSNKLVIEEAERSIHDALCVIRCLVKKRALIAGGGAPEIELALRLTEYSRTLSGMESYC VRAFADAMEVIPSTLAENAGLNPISTVTELRNRHAQGEKTAGINVRKGGISNILEELVVQPLLVSVSALT LATETVRSILKIDDVVNTR TRTRPLE - GFP Tag - V |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_001256721 |
| ORF Size | 1527 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_001256721.1, NP_001243650.1 |
| RefSeq Size | 2262 bp |
| RefSeq ORF | 1530 bp |
| Locus ID | 10575 |
| UniProt ID | P50991 |
| Protein Families | Druggable Genome |
| Gene Summary | The chaperonin containing TCP1 (MIM 186980) complex (CCT), also called the TCP1 ring complex, consists of 2 back-to-back rings, each containing 8 unique but homologous subunits, such as CCT4. CCT assists the folding of newly translated polypeptide substrates through multiple rounds of ATP-driven release and rebinding of partially folded intermediate forms. Substrates of CCT include the cytoskeletal proteins actin (see MIM 102560) and tubulin (see MIM 191130), as well as alpha-transducin (MIM 139330) (Won et al., 1998 [PubMed 9819444]).[supplied by OMIM, Mar 2008] |
Documents
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| SDS |
Resources
Other Versions
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