TCP1 delta (CCT4) (NM_001256721) Human Untagged Clone

Specifications

Product Data
Type	Human Untagged Clone
Tag	Tag Free
Synonyms	CCT-DELTA; Cctd; SRB
Vector	pCMV6-Entry
Sequence Data	Fully Sequenced ORF (showhide) >SC332451 representing NM_001256721. Blue=Insert sequence Red=Cloning site Green=Tag(s) ATGCCCGAGAATGTGGCACCCCGGAGCGGGGCGACTGCCGGGGCTGCCGGCGGCCGCGGGAAAGGCGCC TATCAGGACCGCGACAAGCCAGCCCAGATCCGCTTCAGCAACATTTCCGCCGCCAAAGCGGTTGCTGAT GCTATTAGAACAAGCCTTGGACCAAAAGGAATGGATAAAATGCTGGTGGAGCTGTCTAAGGCTCAAGAT ATAGAAGCAGGAGATGGCACCACATCAGTAGTCATCATTGCTGGCTCCCTCTTAGATTCTTGTACCAAG CTTCTTCAGAAAGGGATTCATCCAACCATCATTTCTGAGTCATTCCAGAAGGCCCTGGAAAAGGGCATT GAAATCTTGACTGACATGTCTCGACCTGTGGAACTGAGTGACAGAGAAACTTTGTTAAATAGTGCAACC ACTTCACTGAACTCAAAGGTGGTTTCTCAGTATTCAAGTCTGCTTTCTCCAATGAGTGTAAATGCAGTG ATGAAAGTGATTGACCCAGCCACAGCCACCAGTGTAGATCTTAGAGATATTAAAATAGTTAAGAAGCTT GGTGGGACAATTGATGACTGTGAGTTGGTGGAAGGGCTGGTTCTCACCCAAAAAGTGTCAAATTCTGGC ATAACCAGAGTTGAAAAGGCCAAGATTGGGCTTATTCAGTTTTGCTTATCTGCTCCCAAAACAGACATG GATAATCAAATAGTGGTTTCTGACTATGCCCAGATGGACCGAGTGCTGCGAGAAGAGAGAGCCTATATT TTAAATTTAGTGAAGCAAATTAAAAAAACAGGATGTAATGTCCTTCTCATACAGAAATCTATTCTAAGA GATGCTCTTAGTGATCTTGCATTACACTTTCTGAATAAAATGAAGATCATGGTGATTAAGGATATTGAA AGAGAAGACATTGAATTCATTTGTAAGACAATTGGAACCAAGCCAGTTGCTCATATTGACCAATTTACT GCTGACATGCTGGGTTCTGCTGAGTTAGCTGAGGAGGTCAATTTAAATGGTTCTGGCAAACTGCTCAAG ATTACAGGCTGTGCCAGCCCTGGAAAAACAGTTACAATTGTTGTTCGTGGTTCTAACAAACTGGTGATT GAAGAAGCTGAGCGCTCCATTCATGATGCCCTATGTGTTATTCGTTGTTTAGTGAAGAAGAGGGCTCTT ATTGCAGGAGGTGGTGCTCCAGAAATAGAGTTGGCCCTACGATTAACTGAATATTCACGAACACTGAGT GGTATGGAATCCTACTGCGTTCGTGCTTTTGCAGATGCTATGGAGGTCATTCCATCTACACTAGCTGAA AATGCCGGCCTGAATCCCATTTCTACAGTAACAGAACTAAGAAACCGGCATGCCCAGGGAGAAAAAACT GCAGGCATTAATGTCCGAAAGGGTGGTATTTCCAACATTTTGGAGGAACTGGTTGTCCAGCCTCTGTTG GTATCAGTCAGTGCTCTGACTCTTGCAACTGAAACTGTTCGGAGCATTCTGAAAATAGATGATGTGGTA AACACTCGATAA
Restriction Sites	SgfI-MluI
ACCN	NM_001256721
Insert Size	1530 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	NM_001256721.1
RefSeq Size	2262 bp
RefSeq ORF	1530 bp
Locus ID	10575
UniProt ID	P50991
Protein Families	Druggable Genome
MW	54.7 kDa
Gene Summary	The chaperonin containing TCP1 (MIM 186980) complex (CCT), also called the TCP1 ring complex, consists of 2 back-to-back rings, each containing 8 unique but homologous subunits, such as CCT4. CCT assists the folding of newly translated polypeptide substrates through multiple rounds of ATP-driven release and rebinding of partially folded intermediate forms. Substrates of CCT include the cytoskeletal proteins actin (see MIM 102560) and tubulin (see MIM 191130), as well as alpha-transducin (MIM 139330) (Won et al., 1998 [PubMed 9819444]).[supplied by OMIM, Mar 2008] Transcript Variant: This variant (2) lacks an in-frame exon in the coding region, compared to variant. It encodes isoform b which is shorter than isoform a.

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