GPR176 (NM_001271854) Human Tagged ORF Clone
CAT#: RG234325
- TrueORF®
GPR176 (tGFP-tagged) - Homo sapiens G protein-coupled receptor 176 (GPR176), transcript variant 2
ORF Plasmid: DDK
"NM_001271854" in other vectors (2)
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CNY 5,040.00
Specifications
Product Data | |
Type | Human Tagged ORF Clone |
Tag | TurboGFP |
Synonyms | HB-954 |
Vector | pCMV6-AC-GFP |
E. coli Selection | Ampicillin (100 ug/mL) |
Mammalian Cell Selection | Neomycin |
Sequence Data |
>RG234325 representing NM_001271854
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGGACATAACGGGAGCTGGATCTCTCCAAATGCCAGCGAGCCGCACAACGCGTCCGGCGCCGAGGCTG CGGGTGTGAACCGCAGCGCGCTCGGGGAGTTCGGCGAGGCGCAGCTGTACCGCCAGTTCACCACCACCGT GCAGGTCGTCATCTTCATAGGCTCGCTGCTCGAGTTTGGCAACATGGAGGTCACTAGAAAACTTGATAAG AGCAGACTGCCTGGGATTAAGTTCATTAAAAACCTGGCCTGCTCGGGGATTTGTGCCAGCCTGGTCTGTG TGCCCTTCGACATCATCCTCAGCACCAGTCCTCACTGTTGCTGGTGGATCTACACCATGCTCTTCTGCAA GGTCGTCAAATTTTTGCACAAAGTATTCTGCTCTGTGACCATCCTCAGCTTCCCTGCTATTGCTTTGGAC AGGTACTACTCAGTCCTCTATCCACTGGAGAGGAAAATATCTGATGCCAAGTCCCGTGAACTGGTGATGT ACATCTGGGCCCATGCAGTGGTGGCCAGTGTCCCTGTGTTTGCAGTAACCAATGTGGCTGACATCTATGC CACGTCCACCTGCACGGAAGTCTGGAGCAACTCCTTGGGCCACCTGGTGTACGTTCTGGTGTATAACATC ACCACGGTCATTGTGCCTGTGGTGGTGGTGTTCCTCTTCTTGATACTGATCCGACGGGCCCTGAGTGCCA GCCAGAAGAAGAAGGTCATCATAGCAGCGCTCCGGACCCCACAGAACACCATCTCTATTCCCTATGCCTC CCAGCGGGAGGCCGAGCTGCACGCCACCCTGCTCTCCATGGTGATGGTCTTCATCTTGTGTAGCGTGCCC TATGCCACCCTGGTCGTCTACCAGACTGTGCTCAATGTCCCTGACACTTCCGTCTTCTTGCTGCTCACTG CTGTTTGGCTGCCCAAAGTCTCCCTGCTGGCAAACCCTGTTCTCTTTCTTACTGTGAACAAATCTGTCCG CAAGTGCTTGATAGGGACCCTGGTGCAACTACACCACCGGTACAGTCGCCGTAATGTGGTCAGTACAGGG AGTGGCATGGCTGAGGCCAGCCTGGAACCCAGCATACGCTCGGGTAGCCAGCTCCTGGAGATGTTCCACA TTGGGCAGCAGCAGATCTTTAAGCCCACAGAGGATGAGGAAGAGAGTGAGGCCAAGTACATTGGCTCAGC TGACTTCCAGGCCAAGGAGATATTTAGCACCTGCCTGGAGGGAGAGCAGGGGCCACAGTTTGCGCCCTCT GCCCCACCCCTGAGCACAGTGGACTCTGTATCCCAGGTGGCACCGGCAGCCCCTGTGGAACCTGAAACAT TCCCTGATAAGTATTCCCTGCAGTTTGGCTTTGGGCCTTTTGAGTTGCCTCCTCAGTGGCTCTCAGAGAC CCGAAACAGCAAGAAGCGGCTGCTTCCCCCCTTGGGCAACACCCCAGAAGAGCTGATCCAGACAAAGGTG CCCAAGGTAGGCAGGGTGGAGCGGAAGATGAGCAGAAACAATAAAGTGAGCATTTTTCCAAAGGTGGATT CC AGCGGACCGACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA >RG234325 representing NM_001271854
Red=Cloning site Green=Tags(s) MGHNGSWISPNASEPHNASGAEAAGVNRSALGEFGEAQLYRQFTTTVQVVIFIGSLLEFGNMEVTRKLDK SRLPGIKFIKNLACSGICASLVCVPFDIILSTSPHCCWWIYTMLFCKVVKFLHKVFCSVTILSFPAIALD RYYSVLYPLERKISDAKSRELVMYIWAHAVVASVPVFAVTNVADIYATSTCTEVWSNSLGHLVYVLVYNI TTVIVPVVVVFLFLILIRRALSASQKKKVIIAALRTPQNTISIPYASQREAELHATLLSMVMVFILCSVP YATLVVYQTVLNVPDTSVFLLLTAVWLPKVSLLANPVLFLTVNKSVRKCLIGTLVQLHHRYSRRNVVSTG SGMAEASLEPSIRSGSQLLEMFHIGQQQIFKPTEDEEESEAKYIGSADFQAKEIFSTCLEGEQGPQFAPS APPLSTVDSVSQVAPAAPVEPETFPDKYSLQFGFGPFELPPQWLSETRNSKKRLLPPLGNTPEELIQTKV PKVGRVERKMSRNNKVSIFPKVDS SGPTRTRRLE - GFP Tag - V |
Restriction Sites |
SgfI-RsrII
Cloning Scheme for this gene
Plasmid Map
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ACCN | NM_001271854 |
ORF Size | 1542 bp |
OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
Reference Data | |
RefSeq | NM_001271854.2 |
RefSeq Size | 4539 bp |
RefSeq ORF | 1545 bp |
Locus ID | 11245 |
UniProt ID | Q14439 |
Protein Families | Druggable Genome, GPCR, Transmembrane |
Gene Summary | Members of the G protein-coupled receptor family, such as GPR176, are cell surface receptors involved in responses to hormones, growth factors, and neurotransmitters (Hata et al., 1995 [PubMed 7893747]).[supplied by OMIM, Jul 2008] |
Documents
Product Manuals |
FAQs |
SDS |