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PGBD5 (NM_001258311) Human Tagged ORF Clone
CAT＃: RG234356
- TrueORF®
PGBD5 (tGFP-tagged) - Homo sapiens piggyBac transposable element derived 5 (PGBD5)
ORF Plasmid: DDK
"NM_001258311" in other vectors (2)
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CNY 5,700.00
Product images
Specifications
| Product Data | |
| Type | Human Tagged ORF Clone |
| Tag | TurboGFP |
| Vector | pCMV6-AC-GFP |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>RG234356 representing NM_001258311
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGCCGAGGGCGGCGGGGGCGCGCGGAGGAGGGCGCCGGCGCTGCTCGAGGCTGCCCGCGCGCGCTACG AGAGCCTGCACATCTCGGACGACGTGTTCGGCGAGTCCGGCCCGGACAGCGGCGGGAACCCCTTCTACAG CACCTCGGCCGCCTCGCGCTCCTCCTCGGCCGCCTCCTCGGACGACGAGCGCGAGCCCCCGGGACCCCCA GGGGCCGCCCCGCCACCGCCCCGCGCCCCGGACGCACAGGAGCCGGAGGAGGACGAGGCCGGCGCGGGCT GGAGCGCAGCGCTGCGGGACCGCCCGCCCCCGCGCTTCGAGGATACCGGCGGTCCCACCCGAAAGATGCC CCCCAGCGCCAGTGCCGTGGACTTCTTCCAGCTCTTTGTCCCAGACAACGTCCTCAAGAACATGGTGGTG CAGACAAACATGTATGCCAAGAAGTTCCAGGAGCGGTTTGGGAGCGACGGAGCCTGGGTGGAGGTGACGC TGACGGAGATGAAGGCGTTCCTGGGCTACATGATCTCCACCAGCATCTCCCACTGCGAGTCCGTCCTCAG CATCTGGAGCGGAGGCTTCTACAGCAACCGCAGCCTCGCCCTCGTCATGAGCCAGGCCCGCTTCGAGAAG ATCCTCAAGTACTTCCACGTCGTGGCCTTCCGCTCCAGCCAGACCACGCACGGGCTCTACAAGGTCCAGC CCTTCCTCGACTCCCTGCAGAACAGCTTCGACTCTGCCTTCAGGCCTTCCCAAACCCAGGTGCTACATGA ACCCCTGATCGATGAGGATCCTGTATTCATTGCCACGTGCACAGAGCGGGAGCTGCGAAAGAGGAAAAAG CGGAAATTCAGCCTCTGGGTCAGACAATGTTCTTCCACTGGCTTCATCATCCAGATTTATGTCCACCTGA AGGAAGGTGGGGGCCCAGATGGCCTGGATGCGCTGAAGAATAAGCCCCAGCTCCACAGCATGGTGGCCAG GAGCCTGTGCCGGAACGCGGCAGGCAAGAACTACATCATTTTCACGGGGCCCAGCATCACCAGCCTGACG CTGTTTGAAGAGTTTGAGAAGCAAGGGATTTACTGCTGCGGCTTGCTCCGCGCGCGGAAGAGTGACTGCA CCGGCCTCCCACTGTCCATGCTGACCAACCCAGCCACACCCCCGGCCCGGGGCCAGTACCAAATCAAGAT GAAGGGGAACATGTCCTTGATCTGCTGGTACAACAAAGGACACTTCCGCTTCCTGACCAACGCCTACTCC CCGGTGCAGCAGGGAGTCATCATCAAAAGGAAGAGTGGGGAGATCCCATGCCCCTTGGCCGTGGAGGCGT TTGCCGCTCACCTGAGCTACATCTGCAGATACGATGACAAATACAGCAAGTATTTCATTTCTCATAAACC AAACAAGACCTGGCAGCAGGTGTTCTGGTTCGCCATCAGCATCGCCATCAACAATGCCTACATCCTGTAC AAAATGTCAGACGCCTACCACGTGAAGAGGTACAGCCGGGCGCAGTTTGGAGAGAGACTCGTCAGAGAGC TGCTGGGCTTGGAGGATGCCTCTCCGACCCAC ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA >RG234356 representing NM_001258311
Red=Cloning site Green=Tags(s) MAEGGGGARRRAPALLEAARARYESLHISDDVFGESGPDSGGNPFYSTSAASRSSSAASSDDEREPPGPP GAAPPPPRAPDAQEPEEDEAGAGWSAALRDRPPPRFEDTGGPTRKMPPSASAVDFFQLFVPDNVLKNMVV QTNMYAKKFQERFGSDGAWVEVTLTEMKAFLGYMISTSISHCESVLSIWSGGFYSNRSLALVMSQARFEK ILKYFHVVAFRSSQTTHGLYKVQPFLDSLQNSFDSAFRPSQTQVLHEPLIDEDPVFIATCTERELRKRKK RKFSLWVRQCSSTGFIIQIYVHLKEGGGPDGLDALKNKPQLHSMVARSLCRNAAGKNYIIFTGPSITSLT LFEEFEKQGIYCCGLLRARKSDCTGLPLSMLTNPATPPARGQYQIKMKGNMSLICWYNKGHFRFLTNAYS PVQQGVIIKRKSGEIPCPLAVEAFAAHLSYICRYDDKYSKYFISHKPNKTWQQVFWFAISIAINNAYILY KMSDAYHVKRYSRAQFGERLVRELLGLEDASPTH TRTRPLE - GFP Tag - V |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_001258311 |
| ORF Size | 1572 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_001258311.2 |
| RefSeq Size | 3373 bp |
| RefSeq ORF | 1575 bp |
| Locus ID | 79605 |
| UniProt ID | Q8N414 |
| Gene Summary | The piggyBac family of proteins, found in diverse animals, are transposases related to the transposase of the canonical piggyBac transposon from the moth, Trichoplusia ni. This family also includes genes in several genomes, including human, that appear to have been derived from the piggyBac transposons. This gene belongs to the subfamily of piggyBac transposable element derived (PGBD) genes. The PGBD proteins appear to be novel, with no obvious relationship to other transposases, or other known protein families. [provided by RefSeq, May 2010] |
Documents
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| FAQs |
| SDS |
