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ZNF195 (NM_001256825) Human Tagged ORF Clone
CAT＃: RG234437
- TrueORF®
ZNF195 (tGFP-tagged) - Homo sapiens zinc finger protein 195 (ZNF195), transcript variant 12
ORF Plasmid: DDK
"NM_001256825" in other vectors (2)
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CNY 5,040.00
Product images
Specifications
| Product Data | |
| Type | Human Tagged ORF Clone |
| Tag | TurboGFP |
| Synonyms | HRF1; ZNFP104 |
| Vector | pCMV6-AC-GFP |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>RG234437 representing NM_001256825
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGCTGGTGCTCAGACTCTGTTGACGTTCAGGGATGTGGCCATAGAATTCTCCCTGGAGGAGTGGAAAT GCCTGGACCTCGCTCAGCAGAATTTGTACAGGGATGTGATGTTGGAGAACTACAGAAACTTGTTCTCCGT TGGTCTCACTGTCTGTAAGCCAGGCCTGATCACCTGCCTGGAGCAACGAAAAGAGCCCTGGAATGTGAAG AGACAGGAGGCAGCAGACGGACATCCAGCTATGTCTTCTCATTTTACCCAAGACCTTCTGCCAGAGCAGG GCATACAAGATGCATTCCCAAAAAGAATACTGAGAGGATATGGAAATTGTGGCCTTGATAATTTATATTT AAGGAAAGACTGGGAAAGTTTAGATGAGTGTAAGTTGCAAAAAGATTATAATGGACTTAACCAATGTTCA TCAACTACCCATAGCAAAATCTTTCAATATAATAAATATGTTAAAATCTTTGATAACTTTTCAAATTTAC ATAGACGTAATATAAGTAATACTGGAGAGAAACCTTTCAAATGTCAAGAATGTGGCAAATCCTTTCAAAT GCTCTCATTCCTAACTGAACATCAGAAAATTCACACTGGAAAAAAATTCCAAAAATGTGGAGAATGTGGC AAAACCTTTATCCAGTGCTCACACTTTACTGAACCTGAGAACATTGACACTGGAGAGAAACCTTACAAGT GTCAAGAATGTAACAACGTCATTAAAACTTGCTCAGTCCTTACTAAAAATAGAATTTATGCCGGAGGGGA ACATTACAGATGTGAAGAATTTGGCAAAGTATTTAACCAGTGCTCCCACCTTACTGAACATGAGCATGGT ACTGAGGAAAAACCCTGCAAATATGAAGAGTGCAGCAGTGTCTTTATCTCTTGCTCAAGCCTTTCTAATC AACAGATGATTCTTGCTGGAGAGAAGCTCTCCAAATGTGAAACATGGTACAAAGGTTTTAACCACAGCCC AAATCCTTCCAAACACCAGAGAAATGAGATTGGAGGGAAACCTTTCAAATGTGAGGAATGTGACAGCATC TTCAAGTGGTTCTCAGACCTTACTAAACATAAGAGAATTCACACTGGTGAGAAACCATACAAATGTGACG AATGTGGGAAAGCCTATACACAGTCCTCACACCTCAGTGAACACAGGAGGATTCACACCGGAGAGAAACC CTACCAATGTGAAGAATGTGGGAAGGTCTTCAGAACTTGCTCAAGCCTTTCTAACCATAAGAGAACTCAT TCTGAAGAAAAACCCTACACGTGTGAAGAATGTGGCAACATCTTTAAGCAGTTATCAGACCTCACTAAGC ATAAGAAAACCCATACTGGAGAGAAGCCCTACAAATGTGACGAATGTGGAAAAAACTTTACCCAGTCCTC CAACCTTATTGTACATAAGAGAATTCATACTGGAGAGAAACCCTACAAGTGTGAAGAATGTGGCAGAGTC TTCATGTGGTTCTCAGACATTACCAAACATAAGAAAACCCATACTGGAGAGAAACCCTACAAATGTGACG AATGTGGAAAAAACTTTACCCAGTCCTCAAACCTTATTGTACATAAGAGAATTCATACTGGAGAGAAACC CTACAAGTGTGAAAAGTGTGGCAAAGCCTTCACCCAGTTCTCACACCTGACTGTACATGAAAGCATTCAT ACT ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA >RG234437 representing NM_001256825
Red=Cloning site Green=Tags(s) MAGAQTLLTFRDVAIEFSLEEWKCLDLAQQNLYRDVMLENYRNLFSVGLTVCKPGLITCLEQRKEPWNVK RQEAADGHPAMSSHFTQDLLPEQGIQDAFPKRILRGYGNCGLDNLYLRKDWESLDECKLQKDYNGLNQCS STTHSKIFQYNKYVKIFDNFSNLHRRNISNTGEKPFKCQECGKSFQMLSFLTEHQKIHTGKKFQKCGECG KTFIQCSHFTEPENIDTGEKPYKCQECNNVIKTCSVLTKNRIYAGGEHYRCEEFGKVFNQCSHLTEHEHG TEEKPCKYEECSSVFISCSSLSNQQMILAGEKLSKCETWYKGFNHSPNPSKHQRNEIGGKPFKCEECDSI FKWFSDLTKHKRIHTGEKPYKCDECGKAYTQSSHLSEHRRIHTGEKPYQCEECGKVFRTCSSLSNHKRTH SEEKPYTCEECGNIFKQLSDLTKHKKTHTGEKPYKCDECGKNFTQSSNLIVHKRIHTGEKPYKCEECGRV FMWFSDITKHKKTHTGEKPYKCDECGKNFTQSSNLIVHKRIHTGEKPYKCEKCGKAFTQFSHLTVHESIH T TRTRPLE - GFP Tag - V |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_001256825 |
| ORF Size | 1683 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_001256825.1, NP_001243754.1 |
| RefSeq Size | 3165 bp |
| RefSeq ORF | 1686 bp |
| Locus ID | 7748 |
| UniProt ID | O14628 |
| Protein Families | Transcription Factors |
| Gene Summary | This gene encodes a protein belonging to the Krueppel C2H2-type zinc-finger protein family. These family members are transcription factors that are implicated in a variety of cellular processes. This gene is located near the centromeric border of chromosome 11p15.5, next to an imprinted domain that is associated with maternal-specific loss of heterozygosity in Wilms' tumors. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Feb 2012] |
Documents
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| FAQs |
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