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BCAR3 (NM_001261410) Human Tagged ORF Clone
CAT＃: RG234795
- TrueORF®
BCAR3 (tGFP-tagged) - Homo sapiens breast cancer anti-estrogen resistance 3 (BCAR3), transcript variant 4
ORF Plasmid: DDK
"NM_001261410" in other vectors (2)
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CNY 6,370.00
货期*
5周
规格
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经常一起买 (5)
Specifications
| Product Data | |
| Type | Human Tagged ORF Clone |
| Tag | TurboGFP |
| Synonyms | AND-34; MIG7; NSP2; SH2D3B |
| Vector | pCMV6-AC-GFP |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>RG234795 representing NM_001261410
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGCCTAAGGAATGCAGTGCTTTCCACGCCCTCTCAGCAGCTCTGTGCTGCTTCTATCACCGCAAGTCTT TCATTGGAGTCAAGTTCTCCAAGGAGAGGCACATCATGGACAGGACCCCCGAGAAACTGAAGAAGGAGCT GGAGGAGGAGCTGCTCCTGAGCAGCGAGGACCTGCGCAGCCATGCCTGGTACCACGGCCGCATCCCCCGA CAGGTGTCTGAAAACCTTGTGCAGCGAGATGGTGACTTCCTAGTTCGTGACTCTCTGTCCAGCCCTGGGA ACTTTGTCCTGACCTGTCAGTGGAAGAACCTCGCTCAGCACTTCAAAATCAACCGGACAGTTCTGCGACT CAGCGAGGCCTACAGCCGCGTGCAGTACCAGTTCGAGATGGAGAGCTTCGACTCCATCCCCGGCCTGGTG CGCTGCTACGTGGGCAACCGCCGGCCCATCTCCCAGCAGAGTGGCGCCATCATCTTCCAGCCCATCAACA GGACGGTGCCTCTGCGGTGCCTGGAGGAGCATTATGGCACCTCCCCAGGCCAGGCCCGGGAGGGCAGCCT CACCAAGGGAAGGCCGGATGTGGCCAAGAGGCTGAGCCTCACCATGGGTGGCGTCCAGGCCCGAGAGCAG AATTTGCCCAGGGGAAACCTCCTCAGAAACAAAGAAAAGAGTGGTAGCCAGCCCGCCTGCCTGGATCACA TGCAGGACAGAAGAGCCTTGTCCCTCAAAGCCCACCAGTCAGAGAGCTACCTGCCGATTGGCTGCAAGCT GCCACCTCAGTCCTCGGGTGTGGACACAAGCCCCTGCCCAAACTCACCTGTGTTCAGGACGGGAAGCGAG CCTGCCCTGAGCCCAGCAGTGGTTCGGAGGGTCTCCTCAGACGCCAGGGCTGGGGAGGCGCTGAGGGGAT CAGACAGTCAACTGTGCCCTAAGCCCCCGCCTAAGCCCTGCAAGGTGCCGTTCCTCAAGGTTCCCTCGTC TCCCTCTGCCTGGCTCAACTCAGAGGCCAACTACTGTGAACTGAACCCAGCGTTTGCCACAGGCTGCGGC AGGGGAGCAAAGCTACCCTCATGTGCCCAGGGAAGCCACACAGAACTGCTCACAGCCAAGCAGAATGAGG CGCCAGGTCCCCGGAACTCTGGCGTCAACTACTTGATCCTTGATGATGATGACAGGGAAAGACCTTGGGA ACCTGCGGCAGCTCAGATGGAGAAGGGGCAGTGGGACAAGGGCGAGTTTGTGACGCCCCTCCTGGAGACT GTCTCCTCCTTCAGGCCCAACGAGTTTGAGTCAAAGTTCCTTCCCCCTGAGAATAAGCCCCTGGAAACAG CAATGTTGAAACGTGCAAAAGAACTGTTCACCAACAACGACCCCAAGGTCATCGCCCAGCACGTACTGAG CATGGACTGCAGGGTTGCTAGGATACTTGGAGTCTCTGAAGAGATGAGGAGGAACATGGGGGTGAGCTCA GGCCTGGAACTCATTACCTTGCCTCACGGACACCAGCTGCGCCTGGACATAATTGAAAGACACAACACAA TGGCCATCGGCATTGCAGTGGACATTCTGGGATGCACGGGCACTTTGGAGGACCGAGCGGCCACTCTGAG TAAGATCATCCAGGTGGCGGTGGAACTGAAGGATTCCATGGGGGACCTCTATTCCTTCTCAGCTCTCATG AAAGCCCTGGAAATGCCACAGATCACAAGGTTAGAAAAGACGTGGACTGCTCTGCGGCACCAGTACACCC AAACTGCCATTCTCTATGAGAAACAGCTGAAGCCCTTCAGCAAACTCCTGCATGAAGGCAGAGAGTCCAC ATGTGTTCCCCCAAACAATGTATCAGTCCCACTGCTGATGCCGCTTGTGACGTTAATGGAGCGCCAGGCT GTGACTTTTGAAGGAACCGACATGTGGGAAAAAAACGACCAGAGCTGTGAAATCATGCTGAACCATTTGG CAACAGCGCGATTCATGGCCGAGGCTGCAGACAGCTACCGGATGAATGCTGAGAGGATCCTGGCAGGTTT TCAACCAGATGAAGAAATGAATGAAATCTGCAAGACTGAATTTCAAATGCGATTGCTATGGGGCAGCAAA GGTGCACAAGTCAATCAGACAGAGAGATATGAGAAATTCAACCAGATTTTAACTGCCCTCTCGCGTAAAT TGGAACCTCCTCCTGTAAAGCAGGCAGAGCTT ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA >RG234795 representing NM_001261410
Red=Cloning site Green=Tags(s) MPKECSAFHALSAALCCFYHRKSFIGVKFSKERHIMDRTPEKLKKELEEELLLSSEDLRSHAWYHGRIPR QVSENLVQRDGDFLVRDSLSSPGNFVLTCQWKNLAQHFKINRTVLRLSEAYSRVQYQFEMESFDSIPGLV RCYVGNRRPISQQSGAIIFQPINRTVPLRCLEEHYGTSPGQAREGSLTKGRPDVAKRLSLTMGGVQAREQ NLPRGNLLRNKEKSGSQPACLDHMQDRRALSLKAHQSESYLPIGCKLPPQSSGVDTSPCPNSPVFRTGSE PALSPAVVRRVSSDARAGEALRGSDSQLCPKPPPKPCKVPFLKVPSSPSAWLNSEANYCELNPAFATGCG RGAKLPSCAQGSHTELLTAKQNEAPGPRNSGVNYLILDDDDRERPWEPAAAQMEKGQWDKGEFVTPLLET VSSFRPNEFESKFLPPENKPLETAMLKRAKELFTNNDPKVIAQHVLSMDCRVARILGVSEEMRRNMGVSS GLELITLPHGHQLRLDIIERHNTMAIGIAVDILGCTGTLEDRAATLSKIIQVAVELKDSMGDLYSFSALM KALEMPQITRLEKTWTALRHQYTQTAILYEKQLKPFSKLLHEGRESTCVPPNNVSVPLLMPLVTLMERQA VTFEGTDMWEKNDQSCEIMLNHLATARFMAEAADSYRMNAERILAGFQPDEEMNEICKTEFQMRLLWGSK GAQVNQTERYEKFNQILTALSRKLEPPPVKQAEL TRTRPLE - GFP Tag - V |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_001261410 |
| ORF Size | 2202 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_001261410.2 |
| RefSeq Size | 2893 bp |
| RefSeq ORF | 2205 bp |
| Locus ID | 8412 |
| UniProt ID | O75815 |
| Protein Families | Druggable Genome |
| Gene Summary | Breast tumors are initially dependent on estrogens for growth and progression and can be inhibited by anti-estrogens such as tamoxifen. However, breast cancers progress to become anti-estrogen resistant. Breast cancer anti-estrogen resistance gene 3 was identified in the search for genes involved in the development of estrogen resistance. The gene encodes a component of intracellular signal transduction that causes estrogen-independent proliferation in human breast cancer cells. The protein contains a putative src homology 2 (SH2) domain, a hall mark of cellular tyrosine kinase signaling molecules, and is partly homologous to the cell division cycle protein CDC48. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, May 2012] |
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