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AKAP13 (NM_001270546) Human Tagged ORF Clone
CAT＃: RG235301
- TrueORF®
AKAP13 (tGFP-tagged) - Homo sapiens A kinase (PRKA) anchor protein 13 (AKAP13), transcript variant 4
ORF Plasmid: DDK
"NM_001270546" in other vectors (2)
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CNY 15,770.00
货期*
14周
规格
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经常一起买 (5)
Specifications
| Product Data | |
| Type | Human Tagged ORF Clone |
| Tag | TurboGFP |
| Synonyms | AKAP-13; AKAP-Lbc; ARHGEF13; BRX; c-lbc; HA-3; Ht31; LBC; p47; PRKA13; PROTO-LB; PROTO-LBC |
| Vector | pCMV6-AC-GFP |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>RG235301 representing NM_001270546
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGTATGAACGGCACAAGAGGCGCTACAGCCTCTGTGACATCTCCAAGGTGGACAGGACTGTGGACGTGG TATTGCTGAAGATAAACCGAGAAAACTGGTGTACAATAGAGCCATGCCCTGATGCAGCATCTCTTCTGGC TTCCAAGCAGAGCCCAGAATGTGAGAACTTCCTGGATGTTGGACTGGGCAGAGAGTGTACCTCAAAACAA GGTGTACTTAAAAGAGAATCTGGGAGTGATTCTGACCTCTTTCACTCACCCAGTGATGACATGGACAGCA TCATCTTCCCAAAGCCAGAGGAAGAGCATTTGGCCTGTGATATCACCGGATCCAGTTCATCCACCGATGA CACGGCTTCACTGGACCGACATTCTTCTCATGGCAGTGATGTGTCTCTCTCCCAGATTTTAAAGCCAAAC AGGTCAAGAGATCGGCAAAGCCTTGATGGATTCTACAGCCATGGGATGGGAGCTGAGGGTCGAGAAAGTG AGAGTGAGCCTGCTGACCCAGGCGACGTGGAGGAGGAGGAGATGGACAGTATCACTGAAGTGCCTGCAAA CTGCTCTGTCCTAAGGAGCTCCATGCGCTCTCTTTCTCCCTTCCGGAGGCACAGCTGGGGGCCTGGGAAA AATGCAGCCAGCGATGCAGAAATGAACCACCGGAGTTTCAGTCTAGAAGGCTTGACAGGAGGAGCTGGTG TCGGAAACAAGCCATCCTCATCTCTAGAAGTAAGCTCTGCAAATGCCGAAGAGCTCAGACACCCATTCAG TGGTGAGGAACGGGTTGACTCTTTGGTGTCACTTTCAGAAGAGGATCTGGAGTCAGACCAGAGAGAACAT AGGATGTTTGATCAGCAGATATGTCACAGATCTAAGCAGCAGGGATTTAATTACTGTACATCAGCCATTT CCTCTCCATTGACAAAATCCATCTCATTAATGACAATCAGCCATCCTGGATTGGACAATTCACGGCCCTT CCACAGTACCTTCCACAATACCAGTGCTAATCTGACTGAGAGTATAACAGAAGAGAACTATAATTTCCTG CCACATAGCCCCTCCAAGAAAGATTCTGAATGGAAGAGTGGAACAAAAGTCAGTCGTACATTCAGCTACA TCAAGAATAAAATGTCTAGCAGCAAGAAGAGCAAAGAAAAGGAAAAAGAAAAAGATAAGATTAAGGAGAA GGAGAAAGATTCTAAAGACAAGGAGAAAGATAAGAAGACTGTCAACGGGCACACTTTCAGTTCCATTCCT GTTGTGGGTCCCATCAGCTGTAGCCAGTGTATGAAGCCCTTCACCAACAAAGATGCCTATACTTGTGCAA ATTGCAGTGCTTTTGTCCACAAAGGCTGCCGAGAAAGTCTAGCCTCCTGTGCAAAGGTCAAAATGAAGCC CAAAGGGAGCCTTCAGGCACATGACACATCATCACTGCCCACGGTCATTATGAGAAACAAGCCCTCACAG CCCAAGGAGCGTCCTCGGTCCGCAGTCCTCCTGGTGGATGAAACCGCTACCACCCCAATATTTGCCAATA GACGATCCCAGCAGAGTGTCTCGCTCTCCAAAAGTGTCTCCATACAGAACATTACTGGAGTTGGCAATGA TGAGAACATGTCAAACACCTGGAAATTCCTGTCTCATTCAACAGACTCACTAAATAAAATCAGCAAGGTC AATGAGTCAACAGAATCACTTACTGATGAGGGAGTAGGTACAGACATGAATGAAGGACAACTACTGGGAG ACTTTGAGATTGAGTCCAAACAGCTGGAAGCAGAGTCTTGGAGTCGGATAATAGACAGCAAGTTTCTAAA ACAGCAAAAGAAAGATGTGGTCAAACGGCAAGAAGTAATATATGAGTTGATGCAGACAGAGTTTCATCAT GTCCGCACTCTCAAGATCATGAGTGGTGTGTACAGCCAGGGGATGATGGCGGATCTGCTTTTTGAGCAGC AGATGGTAGAAAAGCTGTTCCCCTGTTTGGATGAGCTGATCAGTATCCATAGCCAATTCTTCCAGAGGAT TCTGGAGCGGAAGAAGGAGTCTCTGGTGGATAAAAGTGAAAAGAACTTTCTCATCAAGAGGATAGGGGAT GTGCTTGTAAATCAGTTTTCAGGTGAGAATGCAGAACGTTTAAAGAAGACATATGGCAAGTTTTGTGGGC AACATAACCAGTCTGTAAACTACTTCAAAGACCTTTATGCCAAGGATAAGCGTTTTCAAGCCTTTGTAAA GAAGAAGATGAGCAGTTCAGTTGTTAGAAGGCTTGGAATTCCAGAGTGCATATTGCTTGTAACTCAGCGG ATTACCAAGTACCCAGTTTTATTCCAAAGAATATTGCAGTGTACCAAAGACAATGAAGTGGAGCAGGAAG ATCTAGCACAGTCCTTGAGCCTGGTGAAGGATGTGATTGGAGCTGTAGACAGCAAAGTGGCAAGTTATGA AAAGAAAGTGCGTCTCAATGAGATTTATACAAAGACAGATAGCAAGTCAATCATGAGGATGAAGAGTGGT CAGATGTTTGCCAAGGAAGATTTGAAACGGAAGAAGCTTGTACGTGATGGGAGTGTGTTTCTGAAGAATG CAGCAGGAAGGTTGAAAGAGGTTCAAGCAGTTCTTCTCACTGACATTTTAGTTTTCCTTCAAGAAAAAGA CCAGAAGTACATCTTTGCATCATTGGACCAGAAGTCAACAGTGATCTCTTTAAAGAAGCTGATTGTGAGA GAAGTGGCACATGAGGAGAAAGGTTTATTCCTGATCAGCATGGGGATGACAGATCCAGAGATGGTAGAAG TCCATGCCAGCTCCAAAGAGGAACGAAACAGCTGGATTCAGATCATTCAGGACACAATCAACACCCTGAA CAGAGATGAAGATGAAGGAATTCCTAGTGAGAATGAGGAAGAAAAGAAAATGTTGGACACCAGAGCCCGA GAATTAAAAGAACAACTTCACCAGAAGGACCAAAAAATCCTACTCTTGTTGGAAGAGAAGGAGATGATTT TCCGGGACATGGCTGAGTGCAGCACCCCTCTCCCAGAGGATTGCTCCCCAACACATAGCCCTAGAGTTCT CTTCCGCTCCAACACAGAAGAGGCTCTCAAAGGAGGACCTTTAATGAAAAGTGCAATAAATGAGGTGGAG ATCCTTCAGGGTTTGGTGAGTGGAAATCTGGGAGGCACACTTGGGCCGACTGTCAGCAGCCCCATTGAGC AAGATGTGGTCGGTCCCGTTTCCCTGCCCCGGAGAGCAGAGACCTTTGGAGGATTTGACAGCCATCAGAT GAATGCTTCAAAAGGAGGCGAGAAGGAAGAGGGAGATGATGGCCAAGATCTTAGGAGAACGGAATCAGAT AGTGGCCTAAAAAAGGGTGGAAATGCTAACCTGGTATTTATGCTTAAAAGAAACAGTGAGCAGGTTGTCC AGAGCGTTGTTCATCTCTACGAGCTCCTCAGCGCTCTGCAGGGTGTGGTGCTGCAGCAGGACAGCTACAT TGAGGACCAGAAACTGGTGCTGAGCGAGAGGGCGCTCACTCGCAGCTTGTCCCGCCCGAGCTCCCTCATT GAGCAGGAGAAGCAGCGCAGCCTGGAGAAGCAGCGCCAGGACCTGGCCAACCTGCAGAAGCAGCAGGCCC AGTACCTCGAGGAGAAGCGCAGGCGCGAGCGTGAGTGGGAAGCTCGTGAGAGGGAGCTGCGGGAGCGGGA GGCCCTCCTGGCCCAGCGCGAGGAGGAGGTGCAGCAGGGGCAGCAGGACCTGGAAAAGGAGCGGGAGGAG CTCCAGCAGAAGAAGGGCACATACCAGTATGACCTGGAGCGACTGCGTGCTGCCCAGAAACAGCTTGAGA GGGAACAGGAGCAGCTGCGCCGGGAGGCAGAGCGGCTCAGCCAGCGGCAGACAGAACGGGACCTGTGTCA GGTTTCCCATCCACATACCAAGCTGATGAGGATCCCATCGTTCTTCCCCAGTCCTGAGGAGCCCCCCTCG CCATCTGCACCTTCCATAGCCAAATCAGGGTCATTGGACTCAGAACTTTCAGTGTCCCCAAAAAGGAACA GCATCTCTCGGACACACAAAGATAAGGGGCCTTTTCACATACTGAGTTCAACCAGCCAGACAAACAAAGG ACCAGAAGGGCAGAGCCAGGCCCCTGCGTCCACCTCTGCCTCTACCCGCCTGTTTGGGTTAACAAAGCCA AAGGAAAAGAAGGAGAAAAAAAAGAAGAACAAAACCAGCCGCTCTCAGCCCGGTGATGGTCCCGCGTCAG AAGTATCAGCAGAGGGTGAAGAGATCTTCTGC ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA >RG235301 representing NM_001270546
Red=Cloning site Green=Tags(s) MYERHKRRYSLCDISKVDRTVDVVLLKINRENWCTIEPCPDAASLLASKQSPECENFLDVGLGRECTSKQ GVLKRESGSDSDLFHSPSDDMDSIIFPKPEEEHLACDITGSSSSTDDTASLDRHSSHGSDVSLSQILKPN RSRDRQSLDGFYSHGMGAEGRESESEPADPGDVEEEEMDSITEVPANCSVLRSSMRSLSPFRRHSWGPGK NAASDAEMNHRSFSLEGLTGGAGVGNKPSSSLEVSSANAEELRHPFSGEERVDSLVSLSEEDLESDQREH RMFDQQICHRSKQQGFNYCTSAISSPLTKSISLMTISHPGLDNSRPFHSTFHNTSANLTESITEENYNFL PHSPSKKDSEWKSGTKVSRTFSYIKNKMSSSKKSKEKEKEKDKIKEKEKDSKDKEKDKKTVNGHTFSSIP VVGPISCSQCMKPFTNKDAYTCANCSAFVHKGCRESLASCAKVKMKPKGSLQAHDTSSLPTVIMRNKPSQ PKERPRSAVLLVDETATTPIFANRRSQQSVSLSKSVSIQNITGVGNDENMSNTWKFLSHSTDSLNKISKV NESTESLTDEGVGTDMNEGQLLGDFEIESKQLEAESWSRIIDSKFLKQQKKDVVKRQEVIYELMQTEFHH VRTLKIMSGVYSQGMMADLLFEQQMVEKLFPCLDELISIHSQFFQRILERKKESLVDKSEKNFLIKRIGD VLVNQFSGENAERLKKTYGKFCGQHNQSVNYFKDLYAKDKRFQAFVKKKMSSSVVRRLGIPECILLVTQR ITKYPVLFQRILQCTKDNEVEQEDLAQSLSLVKDVIGAVDSKVASYEKKVRLNEIYTKTDSKSIMRMKSG QMFAKEDLKRKKLVRDGSVFLKNAAGRLKEVQAVLLTDILVFLQEKDQKYIFASLDQKSTVISLKKLIVR EVAHEEKGLFLISMGMTDPEMVEVHASSKEERNSWIQIIQDTINTLNRDEDEGIPSENEEEKKMLDTRAR ELKEQLHQKDQKILLLLEEKEMIFRDMAECSTPLPEDCSPTHSPRVLFRSNTEEALKGGPLMKSAINEVE ILQGLVSGNLGGTLGPTVSSPIEQDVVGPVSLPRRAETFGGFDSHQMNASKGGEKEEGDDGQDLRRTESD SGLKKGGNANLVFMLKRNSEQVVQSVVHLYELLSALQGVVLQQDSYIEDQKLVLSERALTRSLSRPSSLI EQEKQRSLEKQRQDLANLQKQQAQYLEEKRRREREWEARERELREREALLAQREEEVQQGQQDLEKEREE LQQKKGTYQYDLERLRAAQKQLEREQEQLRREAERLSQRQTERDLCQVSHPHTKLMRIPSFFPSPEEPPS PSAPSIAKSGSLDSELSVSPKRNSISRTHKDKGPFHILSSTSQTNKGPEGQSQAPASTSASTRLFGLTKP KEKKEKKKKNKTSRSQPGDGPASEVSAEGEEIFC TRTRPLE - GFP Tag - V |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_001270546 |
| ORF Size | 4302 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_001270546.1, NP_001257475.1 |
| RefSeq Size | 9146 bp |
| RefSeq ORF | 4305 bp |
| Locus ID | 11214 |
| UniProt ID | Q12802 |
| Protein Families | Druggable Genome |
| Gene Summary | The A-kinase anchor proteins (AKAPs) are a group of structurally diverse proteins which have the common function of binding to the regulatory subunit of protein kinase A (PKA) and confining the holoenzyme to discrete locations within the cell. This gene encodes a member of the AKAP family. Alternative splicing of this gene results in multiple transcript variants encoding different isoforms containing c-terminal dbl oncogene homology (DH) and pleckstrin homology (PH) domains. The DH domain is associated with guanine nucleotide exchange activation for the Rho/Rac family of small GTP binding proteins, resulting in the conversion of the inactive GTPase to the active form capable of transducing signals. The PH domain has multiple functions. Therefore, these isoforms function as scaffolding proteins to coordinate a Rho signaling pathway, function as protein kinase A-anchoring proteins and, in addition, enhance ligand-dependent activity of estrogen receptors alpha and beta. [provided by RefSeq, Jul 2012] |
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